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Protein

Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial

Gene

Pdha2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2, and thereby links the glycolytic pathway to the tricarboxylic cycle.By similarity

Catalytic activityi

Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.

Cofactori

Enzyme regulationi

Pyruvate dehydrogenase activity is inhibited by phosphorylation of PDHA2; it is reactivated by dephosphorylation.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processCarbohydrate metabolism, Glucose metabolism, Tricarboxylic acid cycle
LigandPyruvate, Thiamine pyrophosphate

Enzyme and pathway databases

ReactomeiR-MMU-204174 Regulation of pyruvate dehydrogenase (PDH) complex
R-MMU-389661 Glyoxylate metabolism and glycine degradation
R-MMU-5362517 Signaling by Retinoic Acid
R-MMU-70268 Pyruvate metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial (EC:1.2.4.1)
Alternative name(s):
PDHE1-A type II
Gene namesi
Name:Pdha2
Synonyms:Pdha-2, Pdhal
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:97533 Pdha2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 30MitochondrionBy similarityAdd BLAST30
ChainiPRO_000002044831 – 391Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrialAdd BLAST361

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei294Phosphoserine; by PDK1, PDK2, PDK3 and PDK4By similarity1
Modified residuei301Phosphoserine; by PDK3By similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP35487
PaxDbiP35487
PeptideAtlasiP35487
PRIDEiP35487

2D gel databases

REPRODUCTION-2DPAGEiP35487

PTM databases

iPTMnetiP35487
PhosphoSitePlusiP35487

Expressioni

Tissue specificityi

Testis.

Gene expression databases

BgeeiENSMUSG00000047674
CleanExiMM_PDHA2
GenevisibleiP35487 MM

Interactioni

Subunit structurei

Heterotetramer of two PDHA2 and two PDHB subunits. The heterotetramer interacts with DLAT, and is part of the multimeric pyruvate dehydrogenase complex that contains multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3). These subunits are bound to an inner core composed of about 48 DLAT and 12 PDHX molecules (By similarity).By similarity

Protein-protein interaction databases

BioGridi202091, 1 interactor
STRINGi10090.ENSMUSP00000060774

Structurei

3D structure databases

ProteinModelPortaliP35487
SMRiP35487
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0225 Eukaryota
COG1071 LUCA
GeneTreeiENSGT00530000063174
HOGENOMiHOG000281336
HOVERGENiHBG001863
InParanoidiP35487
KOiK00161
OMAiDANAQRM
OrthoDBiEOG091G0966
PhylomeDBiP35487
TreeFamiTF300742

Family and domain databases

InterProiView protein in InterPro
IPR001017 DH_E1
IPR017597 Pyrv_DH_E1_asu_subgrp-y
IPR029061 THDP-binding
PfamiView protein in Pfam
PF00676 E1_dh, 1 hit
SUPFAMiSSF52518 SSF52518, 1 hit
TIGRFAMsiTIGR03182 PDH_E1_alph_y, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35487-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKMLTAVLS HVFSGMVQKP ALRGLLSSLK FSNDATCDIK KCDLYRLEEG
60 70 80 90 100
PPTSTVLTRA EALKYYRTMQ VIRRMELKAD QLYKQKFIRG FCHLCDGQEA
110 120 130 140 150
CCVGLEAGIN PTDHVITSYR AHGFCYTRGL SVKSILAELT GRKGGCAKGK
160 170 180 190 200
GGSMHMYGKN FYGGNGIVGA QVPLGAGVAF ACKYLKNGQV CLALYGDGAA
210 220 230 240 250
NQGQVFEAYN MSALWKLPCV FICENNLYGM GTSNERSAAS TDYHKKGFII
260 270 280 290 300
PGLRVNGMDI LCVREATKFA ADHCRSGKGP IVMELQTYRY HGHSMSDPGI
310 320 330 340 350
SYRSREEVHN VRSKSDPIML LRERIISNNL SNIEELKEID ADVKKEVEDA
360 370 380 390
AQFATTDPEP AVEDIANYLY HQDPPFEVRG AHKWLKYKSH S
Length:391
Mass (Da):43,413
Last modified:June 1, 1994 - v1
Checksum:iA2DE823362485977
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M76728 mRNA Translation: AAA53047.1
AK076791 mRNA Translation: BAC36482.1
BC100460 mRNA Translation: AAI00461.1
CCDSiCCDS17872.1
PIRiS23507
RefSeqiNP_032837.1, NM_008811.2
UniGeneiMm.4223

Genome annotation databases

EnsembliENSMUST00000057860; ENSMUSP00000060774; ENSMUSG00000047674
GeneIDi18598
KEGGimmu:18598
UCSCiuc008rob.1 mouse

Similar proteinsi

Entry informationi

Entry nameiODPAT_MOUSE
AccessioniPrimary (citable) accession number: P35487
Secondary accession number(s): Q497M8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: June 20, 2018
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

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