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Protein

Cartilage oligomeric matrix protein

Gene

COMP

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7 (By similarity).By similarity

Cofactori

Ca2+By similarityNote: Binds 11-14 calcium ions per subunit.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHeparin-binding
Biological processApoptosis, Cell adhesion
LigandCalcium

Names & Taxonomyi

Protein namesi
Recommended name:
Cartilage oligomeric matrix protein
Short name:
COMP
Gene namesi
Name:COMP
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000018646021 – 756Cartilage oligomeric matrix proteinAdd BLAST736

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi69InterchainPROSITE-ProRule annotation
Disulfide bondi72InterchainPROSITE-ProRule annotation
Disulfide bondi90 ↔ 101PROSITE-ProRule annotation
Disulfide bondi95 ↔ 110PROSITE-ProRule annotation
Disulfide bondi113 ↔ 124PROSITE-ProRule annotation
Glycosylationi120N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi130 ↔ 141PROSITE-ProRule annotation
Disulfide bondi135 ↔ 150PROSITE-ProRule annotation
Disulfide bondi183 ↔ 196PROSITE-ProRule annotation
Disulfide bondi190 ↔ 205PROSITE-ProRule annotation
Disulfide bondi228 ↔ 242PROSITE-ProRule annotation
Disulfide bondi236 ↔ 252PROSITE-ProRule annotation
Disulfide bondi254 ↔ 265PROSITE-ProRule annotation
Glycosylationi741N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PeptideAtlasiP35445
PRIDEiP35445

Interactioni

Subunit structurei

Pentamer; disulfide-linked. Exists in a more compact conformation in the presence of calcium and shows a more extended conformation in the absence of calcium. Interacts with ITGB3, ITGA5 and FN1. Binding to FN1 requires the presence of divalent cations (Ca2+, Mg2+ or Mn2+). The greatest amount of binding is seen in the presence of Mn2+. Interacts with MATN3, MATN4 and ACAN. Binds heparin, heparan sulfate and chondroitin sulfate. EDTA dimishes significantly its binding to ACAN and abolishes its binding to MATN3, MATN4 and chondroitin sulfate. Interacts with collagen I, II and IX and interaction with these collagens is dependent on the presence of zinc ions (By similarity). Interacts with MATN1. Interacts with ADAMTS12. Interacts with ITGA7 (By similarity).By similarity

Protein-protein interaction databases

IntActiP35445, 2 interactors
MINTiP35445

Structurei

3D structure databases

ProteinModelPortaliP35445
SMRiP35445
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini86 – 125EGF-like 1PROSITE-ProRule annotationAdd BLAST40
Domaini126 – 178EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd BLAST53
Domaini179 – 218EGF-like 3; calcium-bindingPROSITE-ProRule annotationAdd BLAST40
Domaini224 – 266EGF-like 4PROSITE-ProRule annotationAdd BLAST43
Repeati267 – 299TSP type-3 1Add BLAST33
Repeati300 – 335TSP type-3 2Add BLAST36
Repeati336 – 358TSP type-3 3Add BLAST23
Repeati359 – 394TSP type-3 4Add BLAST36
Repeati395 – 417TSP type-3 5Add BLAST23
Repeati418 – 455TSP type-3 6Add BLAST38
Repeati456 – 491TSP type-3 7Add BLAST36
Repeati492 – 527TSP type-3 8Add BLAST36
Domaini531 – 745TSP C-terminalPROSITE-ProRule annotationAdd BLAST215

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni22 – 85COMP N-terminalAdd BLAST64
Regioni526 – 756Mediates cell survival and induction of the IAP family of survival proteinsBy similarityAdd BLAST231

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi366 – 368Cell attachment siteSequence analysis3

Domaini

The cell attachment motif mediates the attachment to chondrocytes. It mediates the induction of both the IAP family of survival proteins and the antiapoptotic response (By similarity).By similarity
The TSP C-terminal domain mediates interaction with FN1 and ACAN.By similarity
Each of the eight TSP type-3 repeats binds two calcium ions. The TSP C-terminal domain binds three calcium ions (By similarity).By similarity

Sequence similaritiesi

Belongs to the thrombospondin family.Curated

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

HOGENOMiHOG000007542
HOVERGENiHBG000636
InParanoidiP35445

Family and domain databases

CDDicd16077 TSP-5cc, 1 hit
Gene3Di4.10.1080.10, 3 hits
InterProiView protein in InterPro
IPR013320 ConA-like_dom_sf
IPR001881 EGF-like_Ca-bd_dom
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR018097 EGF_Ca-bd_CS
IPR024665 Thbs/COMP_coiled-coil
IPR037349 Thrombospondin
IPR003367 Thrombospondin_3-like_rpt
IPR017897 Thrombospondin_3_rpt
IPR008859 Thrombospondin_C
IPR028492 TSP-5
IPR039081 TSP-5_cc
IPR028974 TSP_type-3_rpt
PANTHERiPTHR10199 PTHR10199, 1 hit
PTHR10199:SF88 PTHR10199:SF88, 1 hit
PfamiView protein in Pfam
PF11598 COMP, 1 hit
PF07645 EGF_CA, 2 hits
PF02412 TSP_3, 6 hits
PF05735 TSP_C, 1 hit
SMARTiView protein in SMART
SM00181 EGF, 4 hits
SM00179 EGF_CA, 2 hits
SUPFAMiSSF103647 SSF103647, 3 hits
SSF49899 SSF49899, 1 hit
PROSITEiView protein in PROSITE
PS01186 EGF_2, 1 hit
PS50026 EGF_3, 3 hits
PS01187 EGF_CA, 2 hits
PS51234 TSP3, 8 hits
PS51236 TSP_CTER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35445-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLAAARVLL LTLAALGASG QGQMPLGGDL GPQMLRELQE TNAALQDVRD
60 70 80 90 100
LLRQQVKEIT FLKNTVMECD ACGMQPARTP KLTVRPLSQC SPGFCFPGVA
110 120 130 140 150
CTETANGARC GPCPEGFTGN GSHCADVNEC TAHPCFPRVR CINTSPGFRC
160 170 180 190 200
EACPPGFSGP THEGVGLAFA KANKQVCTDI NECETGQHNC VPNSVCVNTV
210 220 230 240 250
GSFQCGPCQP GFVGDQASGC RRRPQRFCPD GTPSPCHEKA DCVLERDGSR
260 270 280 290 300
SCVCAVGWAG NGLICGRDTD LDGFPDEKLR CSERQCRKDN CVTVPNSGQE
310 320 330 340 350
DVDQDGIGDA CDPDADGDGV LNEKDNCPLV RNPDQRNTDG DKWGDACDNC
360 370 380 390 400
RSQKNDDQKD TDKDGRGDAC DDDIDGDRIR NPVDNCPKVP NSDQKDTDGD
410 420 430 440 450
GVGDACDNCP QKSNADQRDV DHDFVGDACD SDQDQDGDGH QDSKDNCPTV
460 470 480 490 500
PNSAQQDSDH DGQGDACDDD DDNDGVPDSR DNCRLVPNPG QEDMDRDGVG
510 520 530 540 550
DACQGDFDAD KVVDKIDVCP ENAEVTLTDF RAFQTVVLDP EGDAQIDPNW
560 570 580 590 600
VVLNQGMEIV QTMNSDPGLA VGYTAFNGVD FEGTFHVNTA TDDDYAGFIF
610 620 630 640 650
GYQDSSSFYV VMWKQMEQTY WQANPFRAVA EPGIQLKAVK SSTGPGEQLR
660 670 680 690 700
NALWHTGDTA SQVRLLWKDP RNVGWKDKTS YRWFLQHRPQ VGYIRVRFYE
710 720 730 740 750
GPELVADSNV ILDTTMRGGR LGVFCFSQEN IIWANLRYRC NDTIPEDYEA

QRLLQA
Length:756
Mass (Da):82,362
Last modified:April 17, 2007 - v2
Checksum:iDC2D84FE1C18FCA2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti570A → C in CAA52374 (PubMed:1429587).Curated1
Sequence conflicti584T → P in CAA52374 (PubMed:1429587).Curated1
Sequence conflicti603 – 604QD → HH in CAA52374 (PubMed:1429587).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74326 mRNA Translation: CAA52374.1
PIRiB44315
UniGeneiBt.3034

Similar proteinsi

Entry informationi

Entry nameiCOMP_BOVIN
AccessioniPrimary (citable) accession number: P35445
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: April 17, 2007
Last modified: July 18, 2018
This is version 129 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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