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Protein

Thrombospondin-2

Gene

THBS2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Ligand for CD36 mediating antiangiogenic properties.1 Publication

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • heparin binding Source: ProtInc

GO - Biological processi

Keywordsi

Molecular functionHeparin-binding
Biological processCell adhesion
LigandCalcium

Enzyme and pathway databases

ReactomeiR-HSA-186797 Signaling by PDGF
R-HSA-5083635 Defective B3GALTL causes Peters-plus syndrome (PpS)
R-HSA-5173214 O-glycosylation of TSR domain-containing proteins
SIGNORiP35442

Names & Taxonomyi

Protein namesi
Recommended name:
Thrombospondin-2
Gene namesi
Name:THBS2
Synonyms:TSP2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000186340.14
HGNCiHGNC:11786 THBS2
MIMi188061 gene
neXtProtiNX_P35442

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Involvement in diseasei

Intervertebral disc disease (IDD)1 Publication
Disease susceptibility is associated with variations affecting the gene represented in this entry.
Disease descriptionA common musculo-skeletal disorder caused by degeneration of intervertebral disks of the lumbar spine. It results in low-back pain and unilateral leg pain.
See also OMIM:603932

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi702N → S: Alters protein stability. 1 Publication1

Organism-specific databases

DisGeNETi7058
MalaCardsiTHBS2
MIMi603932 phenotype
OpenTargetsiENSG00000186340
PharmGKBiPA36498

Polymorphism and mutation databases

BioMutaiTHBS2
DMDMi215273908

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000003584619 – 1172Thrombospondin-2Add BLAST1154

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi151N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi266InterchainCurated
Disulfide bondi270InterchainCurated
Glycosylationi316N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi330N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi393 ↔ 425By similarity
Disulfide bondi397 ↔ 430By similarity
Disulfide bondi408 ↔ 415By similarity
Disulfide bondi449 ↔ 486By similarity
Disulfide bondi453 ↔ 491By similarity
Glycosylationi457N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi464 ↔ 476By similarity
Disulfide bondi506 ↔ 543By similarity
Disulfide bondi510 ↔ 548By similarity
Disulfide bondi521 ↔ 533By similarity
Disulfide bondi553 ↔ 564Combined sources2 Publications
Disulfide bondi558 ↔ 574Combined sources2 Publications
Disulfide bondi577 ↔ 588Combined sources2 Publications
Glycosylationi584N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi594 ↔ 610Combined sources2 Publications
Disulfide bondi601 ↔ 619Combined sources2 Publications
Disulfide bondi622 ↔ 646Combined sources2 Publications
Disulfide bondi652 ↔ 665Combined sources2 Publications
Disulfide bondi659 ↔ 678Combined sources2 Publications
Disulfide bondi680 ↔ 691Combined sources2 Publications
Disulfide bondi707 ↔ 715Combined sources2 Publications
Glycosylationi710N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi720 ↔ 740Combined sources2 Publications
Disulfide bondi756 ↔ 776Combined sources2 Publications
Disulfide bondi779 ↔ 799Combined sources2 Publications
Disulfide bondi815 ↔ 835Combined sources2 Publications
Disulfide bondi838 ↔ 858Combined sources2 Publications
Disulfide bondi876 ↔ 896Combined sources2 Publications
Disulfide bondi912 ↔ 932Combined sources2 Publications
Disulfide bondi948 ↔ 1169Combined sources2 Publications
Glycosylationi1069N-linked (GlcNAc...) asparagine1 Publication1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiP35442
MaxQBiP35442
PaxDbiP35442
PeptideAtlasiP35442
PRIDEiP35442
ProteomicsDBi55062

PTM databases

iPTMnetiP35442
PhosphoSitePlusiP35442

Miscellaneous databases

PMAP-CutDBiQ5RI52

Expressioni

Tissue specificityi

High expression in invertebral disk tissue.1 Publication

Gene expression databases

BgeeiENSG00000186340
CleanExiHS_THBS2
ExpressionAtlasiP35442 baseline and differential
GenevisibleiP35442 HS

Organism-specific databases

HPAiCAB017716
HPA044304

Interactioni

Subunit structurei

Homotrimer; disulfide-linked. Interacts (via the TSP type I repeats) with CD36; the interaction conveys an antiangiogenic effect. Interacts (via the TSP type I repeats) with HRG; the interaction blocks the antiangiogenic effect of THBS2 with CD36 (By similarity). Can bind to fibrinogen, fibronectin, laminin and type V collagen.By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Cacna2d1P542902EBI-2466249,EBI-2466294From Rattus norvegicus.

Protein-protein interaction databases

BioGridi112916, 13 interactors
ComplexPortaliCPX-1788 Thrombospondin 2 complex
CORUMiP35442
IntActiP35442, 1 interactor
STRINGi9606.ENSP00000355751

Structurei

Secondary structure

11172
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi552 – 555Combined sources4
Beta strandi564 – 566Combined sources3
Beta strandi572 – 574Combined sources3
Beta strandi585 – 587Combined sources3
Turni593 – 596Combined sources4
Beta strandi605 – 607Combined sources3
Beta strandi610 – 612Combined sources3
Beta strandi614 – 619Combined sources6
Beta strandi626 – 628Combined sources3
Beta strandi632 – 636Combined sources5
Helixi637 – 640Combined sources4
Beta strandi646 – 648Combined sources3
Turni651 – 655Combined sources5
Beta strandi663 – 667Combined sources5
Beta strandi673 – 680Combined sources8
Beta strandi684 – 693Combined sources10
Beta strandi697 – 700Combined sources4
Turni706 – 710Combined sources5
Beta strandi712 – 714Combined sources3
Beta strandi734 – 736Combined sources3
Turni738 – 740Combined sources3
Beta strandi746 – 749Combined sources4
Turni751 – 753Combined sources3
Turni755 – 758Combined sources4
Beta strandi767 – 772Combined sources6
Helixi774 – 776Combined sources3
Beta strandi793 – 795Combined sources3
Helixi797 – 799Combined sources3
Beta strandi805 – 808Combined sources4
Turni810 – 812Combined sources3
Beta strandi829 – 831Combined sources3
Helixi833 – 835Combined sources3
Beta strandi851 – 854Combined sources4
Turni856 – 858Combined sources3
Beta strandi866 – 869Combined sources4
Helixi871 – 873Combined sources3
Beta strandi890 – 892Combined sources3
Turni894 – 896Combined sources3
Beta strandi903 – 905Combined sources3
Helixi907 – 909Combined sources3
Turni911 – 914Combined sources4
Beta strandi926 – 928Combined sources3
Helixi930 – 932Combined sources3
Beta strandi939 – 941Combined sources3
Helixi943 – 945Combined sources3
Beta strandi960 – 968Combined sources9
Beta strandi971 – 973Combined sources3
Beta strandi979 – 982Combined sources4
Helixi983 – 985Combined sources3
Beta strandi987 – 990Combined sources4
Beta strandi995 – 1016Combined sources22
Beta strandi1024 – 1033Combined sources10
Beta strandi1036 – 1045Combined sources10
Beta strandi1053 – 1055Combined sources3
Beta strandi1061 – 1069Combined sources9
Helixi1076 – 1083Combined sources8
Beta strandi1084 – 1086Combined sources3
Turni1089 – 1091Combined sources3
Beta strandi1092 – 1097Combined sources6
Beta strandi1110 – 1116Combined sources7
Turni1118 – 1120Combined sources3
Beta strandi1122 – 1129Combined sources8
Beta strandi1132 – 1136Combined sources5
Beta strandi1139 – 1141Combined sources3
Beta strandi1148 – 1156Combined sources9
Beta strandi1159 – 1169Combined sources11

3D structure databases

ProteinModelPortaliP35442
SMRiP35442
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP35442

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini19 – 215Laminin G-likeAdd BLAST197
Domaini318 – 375VWFCPROSITE-ProRule annotationAdd BLAST58
Domaini381 – 431TSP type-1 1PROSITE-ProRule annotationAdd BLAST51
Domaini437 – 492TSP type-1 2PROSITE-ProRule annotationAdd BLAST56
Domaini494 – 549TSP type-1 3PROSITE-ProRule annotationAdd BLAST56
Domaini549 – 589EGF-like 1PROSITE-ProRule annotationAdd BLAST41
Domaini648 – 692EGF-like 2PROSITE-ProRule annotationAdd BLAST45
Repeati693 – 728TSP type-3 1Add BLAST36
Repeati729 – 764TSP type-3 2Add BLAST36
Repeati765 – 787TSP type-3 3Add BLAST23
Repeati788 – 823TSP type-3 4Add BLAST36
Repeati824 – 846TSP type-3 5Add BLAST23
Repeati847 – 884TSP type-3 6Add BLAST38
Repeati885 – 920TSP type-3 7Add BLAST36
Repeati921 – 956TSP type-3 8Add BLAST36
Domaini960 – 1172TSP C-terminalPROSITE-ProRule annotationAdd BLAST213

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni19 – 232Heparin-bindingSequence analysisAdd BLAST214

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi928 – 930Cell attachment siteSequence analysis3

Sequence similaritiesi

Belongs to the thrombospondin family.Curated

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IFQQ Eukaryota
ENOG410XQKE LUCA
GeneTreeiENSGT00850000132273
HOGENOMiHOG000007542
HOVERGENiHBG018006
InParanoidiP35442
KOiK04659
OMAiTEKQVCE
OrthoDBiEOG091G00TV
PhylomeDBiP35442
TreeFamiTF324917

Family and domain databases

Gene3Di2.20.100.10, 3 hits
4.10.1080.10, 2 hits
InterProiView protein in InterPro
IPR013320 ConA-like_dom_sf
IPR001881 EGF-like_Ca-bd_dom
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR024731 EGF_dom
IPR001791 Laminin_G
IPR037349 Thrombospondin
IPR003367 Thrombospondin_3-like_rpt
IPR017897 Thrombospondin_3_rpt
IPR008859 Thrombospondin_C
IPR000884 TSP1_rpt
IPR036383 TSP1_rpt_sf
IPR015455 TSP2
IPR028974 TSP_type-3_rpt
IPR001007 VWF_dom
PANTHERiPTHR10199 PTHR10199, 2 hits
PTHR10199:SF10 PTHR10199:SF10, 2 hits
PfamiView protein in Pfam
PF12947 EGF_3, 1 hit
PF07645 EGF_CA, 1 hit
PF00090 TSP_1, 3 hits
PF02412 TSP_3, 7 hits
PF05735 TSP_C, 1 hit
PF00093 VWC, 1 hit
SMARTiView protein in SMART
SM00181 EGF, 3 hits
SM00179 EGF_CA, 2 hits
SM00209 TSP1, 3 hits
SM00210 TSPN, 1 hit
SM00214 VWC, 1 hit
SUPFAMiSSF103647 SSF103647, 3 hits
SSF49899 SSF49899, 2 hits
SSF82895 SSF82895, 3 hits
PROSITEiView protein in PROSITE
PS01186 EGF_2, 1 hit
PS50026 EGF_3, 2 hits
PS50092 TSP1, 3 hits
PS51234 TSP3, 8 hits
PS51236 TSP_CTER, 1 hit
PS01208 VWFC_1, 1 hit
PS50184 VWFC_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35442-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVWRLVLLAL WVWPSTQAGH QDKDTTFDLF SISNINRKTI GAKQFRGPDP
60 70 80 90 100
GVPAYRFVRF DYIPPVNADD LSKITKIMRQ KEGFFLTAQL KQDGKSRGTL
110 120 130 140 150
LALEGPGLSQ RQFEIVSNGP ADTLDLTYWI DGTRHVVSLE DVGLADSQWK
160 170 180 190 200
NVTVQVAGET YSLHVGCDLI DSFALDEPFY EHLQAEKSRM YVAKGSARES
210 220 230 240 250
HFRGLLQNVH LVFENSVEDI LSKKGCQQGQ GAEINAISEN TETLRLGPHV
260 270 280 290 300
TTEYVGPSSE RRPEVCERSC EELGNMVQEL SGLHVLVNQL SENLKRVSND
310 320 330 340 350
NQFLWELIGG PPKTRNMSAC WQDGRFFAEN ETWVVDSCTT CTCKKFKTIC
360 370 380 390 400
HQITCPPATC ASPSFVEGEC CPSCLHSVDG EEGWSPWAEW TQCSVTCGSG
410 420 430 440 450
TQQRGRSCDV TSNTCLGPSI QTRACSLSKC DTRIRQDGGW SHWSPWSSCS
460 470 480 490 500
VTCGVGNITR IRLCNSPVPQ MGGKNCKGSG RETKACQGAP CPIDGRWSPW
510 520 530 540 550
SPWSACTVTC AGGIRERTRV CNSPEPQYGG KACVGDVQER QMCNKRSCPV
560 570 580 590 600
DGCLSNPCFP GAQCSSFPDG SWSCGSCPVG FLGNGTHCED LDECALVPDI
610 620 630 640 650
CFSTSKVPRC VNTQPGFHCL PCPPRYRGNQ PVGVGLEAAK TEKQVCEPEN
660 670 680 690 700
PCKDKTHNCH KHAECIYLGH FSDPMYKCEC QTGYAGDGLI CGEDSDLDGW
710 720 730 740 750
PNLNLVCATN ATYHCIKDNC PHLPNSGQED FDKDGIGDAC DDDDDNDGVT
760 770 780 790 800
DEKDNCQLLF NPRQADYDKD EVGDRCDNCP YVHNPAQIDT DNNGEGDACS
810 820 830 840 850
VDIDGDDVFN ERDNCPYVYN TDQRDTDGDG VGDHCDNCPL VHNPDQTDVD
860 870 880 890 900
NDLVGDQCDN NEDIDDDGHQ NNQDNCPYIS NANQADHDRD GQGDACDPDD
910 920 930 940 950
DNDGVPDDRD NCRLVFNPDQ EDLDGDGRGD ICKDDFDNDN IPDIDDVCPE
960 970 980 990 1000
NNAISETDFR NFQMVPLDPK GTTQIDPNWV IRHQGKELVQ TANSDPGIAV
1010 1020 1030 1040 1050
GFDEFGSVDF SGTFYVNTDR DDDYAGFVFG YQSSSRFYVV MWKQVTQTYW
1060 1070 1080 1090 1100
EDQPTRAYGY SGVSLKVVNS TTGTGEHLRN ALWHTGNTPG QVRTLWHDPR
1110 1120 1130 1140 1150
NIGWKDYTAY RWHLTHRPKT GYIRVLVHEG KQVMADSGPI YDQTYAGGRL
1160 1170
GLFVFSQEMV YFSDLKYECR DI
Length:1,172
Mass (Da):129,991
Last modified:November 25, 2008 - v2
Checksum:i415BF376F4669F6A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2V → A in AAI50176 (PubMed:15489334).Curated1
Sequence conflicti171 – 173DSF → GPV in AAA03703 (PubMed:8406456).Curated3
Sequence conflicti576S → F in AAA03703 (PubMed:8406456).Curated1
Sequence conflicti1111R → G in AAI46677 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_045842133T → S. Corresponds to variant dbSNP:rs36088849Ensembl.1
Natural variantiVAR_045843375L → F. Corresponds to variant dbSNP:rs35404985Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12350 mRNA Translation: AAA03703.1
M81339 mRNA No translation available.
AK292429 mRNA Translation: BAF85118.1
BX322234 Genomic DNA No translation available.
CH471051 Genomic DNA Translation: EAW47455.1
BC146676 mRNA Translation: AAI46677.1
BC150175 mRNA Translation: AAI50176.1
CCDSiCCDS34574.1
PIRiA47379 TSHUP2
RefSeqiNP_003238.2, NM_003247.3
UniGeneiHs.371147

Genome annotation databases

EnsembliENST00000366787; ENSP00000355751; ENSG00000186340
ENST00000617924; ENSP00000482784; ENSG00000186340
GeneIDi7058
KEGGihsa:7058
UCSCiuc003qwt.5 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiTSP2_HUMAN
AccessioniPrimary (citable) accession number: P35442
Secondary accession number(s): A6H8N1, A7E232, Q5RI52
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: November 25, 2008
Last modified: July 18, 2018
This is version 184 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

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