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Protein

Phosphoribosylformylglycinamidine synthase

Gene

ade2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate (By similarity).By similarity

Catalytic activityi

ATP + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine + L-glutamate.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Phosphoribosylformylglycinamidine synthase (ade2)
  2. Trifunctional purine biosynthetic protein adenosine-3 (ade3), Trifunctional purine biosynthetic protein adenosine-3 (Gart), Trifunctional purine biosynthetic protein adenosine-3 (ade3-RA)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei714ATP; via carbonyl oxygenBy similarity1
Metal bindingi715MagnesiumBy similarity1
Metal bindingi754MagnesiumBy similarity1
Metal bindingi758MagnesiumBy similarity1
Metal bindingi918MagnesiumBy similarity1
Binding sitei920ATPBy similarity1
Active sitei1180NucleophileBy similarity1
Active sitei1310By similarity1
Active sitei1312By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi327 – 338ATPSequence analysisAdd BLAST12
Nucleotide bindingi407 – 409ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processPurine biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-73817 Purine ribonucleoside monophosphate biosynthesis
UniPathwayiUPA00074; UER00128

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylformylglycinamidine synthase (EC:6.3.5.3)
Short name:
FGAM synthase
Short name:
FGAMS
Alternative name(s):
Formylglycinamide ribonucleotide amidotransferase
Short name:
FGAR amidotransferase
Short name:
FGAR-AT
Formylglycinamide ribotide amidotransferase
Protein adenosine-2
Gene namesi
Name:ade2
ORF Names:CG9127
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0000052 ade2

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001004031 – 1354Phosphoribosylformylglycinamidine synthaseAdd BLAST1354

Proteomic databases

PaxDbiP35421
PRIDEiP35421

Expressioni

Gene expression databases

BgeeiFBgn0000052
GenevisibleiP35421 DM

Interactioni

Protein-protein interaction databases

BioGridi60012, 4 interactors
DIPiDIP-22769N
IntActiP35421, 2 interactors
STRINGi7227.FBpp0078850

Structurei

3D structure databases

ProteinModelPortaliP35421
SMRiP35421
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1087 – 1337Glutamine amidotransferase type-1Add BLAST251

Sequence similaritiesi

In the N-terminal section; belongs to the FGAMS family.Curated

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiKOG1907 Eukaryota
COG0046 LUCA
COG0047 LUCA
GeneTreeiENSGT00390000007600
InParanoidiP35421
KOiK01952
OMAiSLSANWM
OrthoDBiEOG091G00SI
PhylomeDBiP35421

Family and domain databases

Gene3Di3.30.1330.10, 2 hits
3.40.50.880, 1 hit
3.90.650.10, 2 hits
HAMAPiMF_00419 PurL_1, 1 hit
InterProiView protein in InterPro
IPR029062 Class_I_gatase-like
IPR017926 GATASE
IPR010073 PRibForGlyAmidine_synth
IPR010918 PurM-like_C_dom
IPR036676 PurM-like_C_sf
IPR016188 PurM-like_N
IPR036921 PurM-like_N_sf
IPR036604 PurS-like_sf
PfamiView protein in Pfam
PF00586 AIRS, 1 hit
PF02769 AIRS_C, 2 hits
SUPFAMiSSF52317 SSF52317, 1 hit
SSF55326 SSF55326, 2 hits
SSF56042 SSF56042, 2 hits
SSF82697 SSF82697, 1 hit
TIGRFAMsiTIGR01735 FGAM_synt, 1 hit
PROSITEiView protein in PROSITE
PS51273 GATASE_TYPE_1, 1 hit

Sequencei

Sequence statusi: Complete.

P35421-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVILRYYDVQ AHSAAEEESV LRRLREEDGA VVSVRMERCY HLEYSAQAEH
60 70 80 90 100
SLALDELLVW LVKQPLSKGQ SLSRQPALQS TGSSQLLLEI GPRFNFSTPY
110 120 130 140 150
STNCVNIFQN LGYSEVRRME TSTRYLVTFG EGSKAPEAAR FVPLLGDRMT
160 170 180 190 200
QCLYTEENTP KASFDEQLPE RQANWHFVPV LEEGRAALER INQELGLAFN
210 220 230 240 250
DYDLDYYHDL FAKELGRNPT TVELFDCAQS NSEHSRHWFF RGRMVIDGVE
260 270 280 290 300
QPKSLIRMIM DTQAHTNPNN TIKFSDNSSA MVGFDHQTIV PSSVVAPGAV
310 320 330 340 350
RLQSVQSDLI FTAETHNMPT AVAPFSGATT GTGGRLRDVQ GVGRGGVPIA
360 370 380 390 400
GTAGYCVGAL HIPGYKQPYE PLDFKYPATF APPLQVLIEA SNGASDYGNK
410 420 430 440 450
FGEPVISGFA LSYGLNSAAD ASQRDEYVKP IMFSGGLGTM PATMREKLPP
460 470 480 490 500
ARGQLLAKIG GPVYRIGVGG GAASSVEIQG SGDAELDFNA VQRGDAEMEN
510 520 530 540 550
KLNRVVRACL DLGEQNPILA IHDQGAGGNG NVLKELVEPG FAGAVIFSKE
560 570 580 590 600
FQLGDPTITA LELWGAEYQE NNAILCNADQ RELLEKICRR ERCPISFVGV
610 620 630 640 650
VTGDGRVTLL EKPAPKDLEQ ALNASNRSEV SPFDLELKYV LGDMPKRTYD
660 670 680 690 700
LKREQTPLKE LSLPKGLLLD EALERVLSLV AVGSKRFLTN KVDRCVGGLI
710 720 730 740 750
AQQQCVGPLQ APLADYALTT VSHFSHSGIA TSIGTQPLKG LLDPAAMARM
760 770 780 790 800
CVAEALSNLV FVKISELADV KCSGNWMWAA KLPGEGARMF DACKELCQIL
810 820 830 840 850
EELHIAIDGG KDSLSMAAKV GGETIKSPGT LVISTYAPCP DVRLKVTPDL
860 870 880 890 900
KGPGAGSKTS LLWINLENSA RLGGSALAQA YAQQGKDTPN LTRSDVLGKA
910 920 930 940 950
FAVTQSLLGD GLIQAGHDVS DGGLLVCVLE MAIGGLSGLR VDLSEPLAKL
960 970 980 990 1000
KNFDKSVEKL NRPELAVLFA EECGWVVEVL DTDLERVRST YEKAGVPNYY
1010 1020 1030 1040 1050
LGVTEGFGLD SRVVLKNGKS ELLDQPLRVL YKKWERTSYE LEKLQANPEC
1060 1070 1080 1090 1100
AEAEYNSLEY RQAPQYRGPQ NVQAELTLKR SSAPVRVAVL REEGVNSERE
1110 1120 1130 1140 1150
MMACLLRANF EVHDVTMSDL LQGTASVSQY RGLIFPGGFS YADTLGSAKG
1160 1170 1180 1190 1200
WAANILHNPR LLPQFEAFKR RQDVFSLGIC NGCQLMTLIG FVGSAKSEVG
1210 1220 1230 1240 1250
ADPDVALLHN KSQRFECRWA TVKIPSNRSI MLGSMKDLVL GCWVAHGEGR
1260 1270 1280 1290 1300
FAFRDEKLIS HLQSEQLVTL QYVDDVGKPT ELYPLNPNGS PQGIAGLCSS
1310 1320 1330 1340 1350
DGRHLALMPH PERCSSMYQW PYVPSSFEVS PTQSESPWQI MFNNAYNWCV

KSNQ
Length:1,354
Mass (Da):148,085
Last modified:January 11, 2001 - v2
Checksum:i8AA167C2D2920B2C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti558I → Y in AAC46468 (PubMed:8270203).Curated1
Sequence conflicti625S → F in AAC46468 (PubMed:8270203).Curated1
Sequence conflicti766E → V in AAR82811 (Ref. 4) Curated1
Sequence conflicti910D → E in AAR82811 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00683 Unassigned DNA Translation: AAC46468.1
AE014134 Genomic DNA Translation: AAF52329.1
AE014134 Genomic DNA Translation: AAN10573.1
AE014134 Genomic DNA Translation: AAN10574.1
BT011143 mRNA Translation: AAR82811.1
PIRiT13363
RefSeqiNP_477212.1, NM_057864.4
NP_723146.1, NM_164675.2
NP_723147.1, NM_164676.2

Genome annotation databases

EnsemblMetazoaiFBtr0079219; FBpp0078850; FBgn0000052
FBtr0079220; FBpp0078851; FBgn0000052
FBtr0079221; FBpp0078852; FBgn0000052
GeneIDi33847
KEGGidme:Dmel_CG9127

Similar proteinsi

Entry informationi

Entry nameiPUR4_DROME
AccessioniPrimary (citable) accession number: P35421
Secondary accession number(s): A4V099, Q6NNY9, Q9VMI7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 11, 2001
Last modified: May 23, 2018
This is version 153 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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