UniProtKB - P35421 (PUR4_DROME)
Protein
Phosphoribosylformylglycinamidine synthase
Gene
Pfas
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway (PubMed:3086869). Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate (PubMed:3086869). Because of its role in metabolisms, is involved in sleep regulation (PubMed:3086869).1 Publication
Catalytic activityi
- ATP + H2O + L-glutamine + N2-formyl-N1-(5-phospho-β-D-ribosyl)glycinamide = 2-formamido-N1-(5-O-phospho-β-D-ribosyl)acetamidine + ADP + H+ + L-glutamate + phosphate1 PublicationEC:6.3.5.31 Publication
: IMP biosynthesis via de novo pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.1 PublicationProteins known to be involved in the 2 steps of the subpathway in this organism are:
- Phosphoribosylformylglycinamidine synthase (Pfas)
- Trifunctional purine biosynthetic protein adenosine-3 (ade3-RA), AIR synthase (gart), Trifunctional purine biosynthetic protein adenosine-3 (Gart), Trifunctional purine biosynthetic protein adenosine-3 (Gart)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 714 | ATP; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 715 | MagnesiumBy similarity | 1 | |
Metal bindingi | 754 | MagnesiumBy similarity | 1 | |
Metal bindingi | 758 | MagnesiumBy similarity | 1 | |
Metal bindingi | 918 | MagnesiumBy similarity | 1 | |
Binding sitei | 920 | ATPBy similarity | 1 | |
Active sitei | 1180 | NucleophileBy similarity | 1 | |
Active sitei | 1310 | By similarity | 1 | |
Active sitei | 1312 | By similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 327 – 338 | ATPSequence analysisAdd BLAST | 12 | |
Nucleotide bindingi | 407 – 409 | ATPBy similarity | 3 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- phosphoribosylformylglycinamidine synthase activity Source: UniProtKB
GO - Biological processi
- 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
- glutamine metabolic process Source: UniProtKB-KW
- purine nucleobase biosynthetic process Source: UniProtKB
- purine nucleotide biosynthetic process Source: FlyBase
Keywordsi
Molecular function | Ligase |
Biological process | Purine biosynthesis |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-DME-73817, Purine ribonucleoside monophosphate biosynthesis |
UniPathwayi | UPA00074;UER00128 |
Names & Taxonomyi
Protein namesi | Recommended name: Phosphoribosylformylglycinamidine synthaseImported (EC:6.3.5.31 Publication)Short name: FGAM synthase Short name: FGAMS Alternative name(s): Formylglycinamide ribonucleotide amidotransferase Short name: FGAR amidotransferase Short name: FGAR-AT Formylglycinamide ribotide amidotransferase Protein adenosine-2 |
Gene namesi | ORF Names:CG9127Imported |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0000052, Pfas |
Subcellular locationi
Other locations
- cytoplasm Source: FlyBase
Pathology & Biotechi
Disruption phenotypei
Pupal lethal (PubMed:21441212). RNAi-mediated knockdown in the fat body reduces energy storage of both triglyceride and free glucose, increases waking activity during the day and the night, reduces sleep bout length, with total sleep bout number reduced during the day and increased during the night (PubMed:30249751).2 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000100403 | 1 – 1354 | Phosphoribosylformylglycinamidine synthaseAdd BLAST | 1354 |
Proteomic databases
PaxDbi | P35421 |
PRIDEi | P35421 |
Expressioni
Gene expression databases
Bgeei | FBgn0000052, Expressed in arthropod fat body and 52 other tissues |
Genevisiblei | P35421, DM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 60012, 4 interactors |
DIPi | DIP-22769N |
IntActi | P35421, 2 interactors |
STRINGi | 7227.FBpp0078850 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1087 – 1337 | Glutamine amidotransferase type-1Add BLAST | 251 |
Sequence similaritiesi
In the N-terminal section; belongs to the FGAMS family.Curated
Keywords - Domaini
Glutamine amidotransferasePhylogenomic databases
eggNOGi | KOG1907, Eukaryota |
GeneTreei | ENSGT00390000007600 |
HOGENOMi | CLU_001031_0_0_1 |
InParanoidi | P35421 |
OMAi | LSANWMW |
OrthoDBi | 38224at2759 |
PhylomeDBi | P35421 |
Family and domain databases
Gene3Di | 3.30.1330.10, 2 hits 3.40.50.880, 1 hit 3.90.650.10, 2 hits |
HAMAPi | MF_00419, PurL_1, 1 hit |
InterProi | View protein in InterPro IPR029062, Class_I_gatase-like IPR040707, FGAR-AT_N IPR017926, GATASE IPR010073, PurL_large IPR041609, PurL_linker IPR010918, PurM-like_C_dom IPR036676, PurM-like_C_sf IPR016188, PurM-like_N IPR036921, PurM-like_N_sf IPR036604, PurS-like_sf |
Pfami | View protein in Pfam PF00586, AIRS, 1 hit PF02769, AIRS_C, 2 hits PF18072, FGAR-AT_linker, 1 hit PF18076, FGAR-AT_N, 1 hit |
SUPFAMi | SSF52317, SSF52317, 1 hit SSF55326, SSF55326, 2 hits SSF56042, SSF56042, 2 hits SSF82697, SSF82697, 1 hit |
TIGRFAMsi | TIGR01735, FGAM_synt, 1 hit |
PROSITEi | View protein in PROSITE PS51273, GATASE_TYPE_1, 1 hit |
i Sequence
Sequence statusi: Complete.
P35421-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVILRYYDVQ AHSAAEEESV LRRLREEDGA VVSVRMERCY HLEYSAQAEH
60 70 80 90 100
SLALDELLVW LVKQPLSKGQ SLSRQPALQS TGSSQLLLEI GPRFNFSTPY
110 120 130 140 150
STNCVNIFQN LGYSEVRRME TSTRYLVTFG EGSKAPEAAR FVPLLGDRMT
160 170 180 190 200
QCLYTEENTP KASFDEQLPE RQANWHFVPV LEEGRAALER INQELGLAFN
210 220 230 240 250
DYDLDYYHDL FAKELGRNPT TVELFDCAQS NSEHSRHWFF RGRMVIDGVE
260 270 280 290 300
QPKSLIRMIM DTQAHTNPNN TIKFSDNSSA MVGFDHQTIV PSSVVAPGAV
310 320 330 340 350
RLQSVQSDLI FTAETHNMPT AVAPFSGATT GTGGRLRDVQ GVGRGGVPIA
360 370 380 390 400
GTAGYCVGAL HIPGYKQPYE PLDFKYPATF APPLQVLIEA SNGASDYGNK
410 420 430 440 450
FGEPVISGFA LSYGLNSAAD ASQRDEYVKP IMFSGGLGTM PATMREKLPP
460 470 480 490 500
ARGQLLAKIG GPVYRIGVGG GAASSVEIQG SGDAELDFNA VQRGDAEMEN
510 520 530 540 550
KLNRVVRACL DLGEQNPILA IHDQGAGGNG NVLKELVEPG FAGAVIFSKE
560 570 580 590 600
FQLGDPTITA LELWGAEYQE NNAILCNADQ RELLEKICRR ERCPISFVGV
610 620 630 640 650
VTGDGRVTLL EKPAPKDLEQ ALNASNRSEV SPFDLELKYV LGDMPKRTYD
660 670 680 690 700
LKREQTPLKE LSLPKGLLLD EALERVLSLV AVGSKRFLTN KVDRCVGGLI
710 720 730 740 750
AQQQCVGPLQ APLADYALTT VSHFSHSGIA TSIGTQPLKG LLDPAAMARM
760 770 780 790 800
CVAEALSNLV FVKISELADV KCSGNWMWAA KLPGEGARMF DACKELCQIL
810 820 830 840 850
EELHIAIDGG KDSLSMAAKV GGETIKSPGT LVISTYAPCP DVRLKVTPDL
860 870 880 890 900
KGPGAGSKTS LLWINLENSA RLGGSALAQA YAQQGKDTPN LTRSDVLGKA
910 920 930 940 950
FAVTQSLLGD GLIQAGHDVS DGGLLVCVLE MAIGGLSGLR VDLSEPLAKL
960 970 980 990 1000
KNFDKSVEKL NRPELAVLFA EECGWVVEVL DTDLERVRST YEKAGVPNYY
1010 1020 1030 1040 1050
LGVTEGFGLD SRVVLKNGKS ELLDQPLRVL YKKWERTSYE LEKLQANPEC
1060 1070 1080 1090 1100
AEAEYNSLEY RQAPQYRGPQ NVQAELTLKR SSAPVRVAVL REEGVNSERE
1110 1120 1130 1140 1150
MMACLLRANF EVHDVTMSDL LQGTASVSQY RGLIFPGGFS YADTLGSAKG
1160 1170 1180 1190 1200
WAANILHNPR LLPQFEAFKR RQDVFSLGIC NGCQLMTLIG FVGSAKSEVG
1210 1220 1230 1240 1250
ADPDVALLHN KSQRFECRWA TVKIPSNRSI MLGSMKDLVL GCWVAHGEGR
1260 1270 1280 1290 1300
FAFRDEKLIS HLQSEQLVTL QYVDDVGKPT ELYPLNPNGS PQGIAGLCSS
1310 1320 1330 1340 1350
DGRHLALMPH PERCSSMYQW PYVPSSFEVS PTQSESPWQI MFNNAYNWCV
KSNQ
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 558 | I → Y in AAC46468 (PubMed:8270203).Curated | 1 | |
Sequence conflicti | 625 | S → F in AAC46468 (PubMed:8270203).Curated | 1 | |
Sequence conflicti | 766 | E → V in AAR82811 (Ref. 4) Curated | 1 | |
Sequence conflicti | 910 | D → E in AAR82811 (Ref. 4) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00683 Unassigned DNA Translation: AAC46468.1 AE014134 Genomic DNA Translation: AAF52329.1 AE014134 Genomic DNA Translation: AAN10573.1 AE014134 Genomic DNA Translation: AAN10574.1 BT011143 mRNA Translation: AAR82811.1 |
PIRi | T13363 |
RefSeqi | NP_477212.1, NM_057864.4 NP_723146.1, NM_164675.2 NP_723147.1, NM_164676.2 |
Genome annotation databases
EnsemblMetazoai | FBtr0079219; FBpp0078850; FBgn0000052 FBtr0079220; FBpp0078851; FBgn0000052 FBtr0079221; FBpp0078852; FBgn0000052 |
GeneIDi | 33847 |
KEGGi | dme:Dmel_CG9127 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00683 Unassigned DNA Translation: AAC46468.1 AE014134 Genomic DNA Translation: AAF52329.1 AE014134 Genomic DNA Translation: AAN10573.1 AE014134 Genomic DNA Translation: AAN10574.1 BT011143 mRNA Translation: AAR82811.1 |
PIRi | T13363 |
RefSeqi | NP_477212.1, NM_057864.4 NP_723146.1, NM_164675.2 NP_723147.1, NM_164676.2 |
3D structure databases
SMRi | P35421 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 60012, 4 interactors |
DIPi | DIP-22769N |
IntActi | P35421, 2 interactors |
STRINGi | 7227.FBpp0078850 |
Proteomic databases
PaxDbi | P35421 |
PRIDEi | P35421 |
Genome annotation databases
EnsemblMetazoai | FBtr0079219; FBpp0078850; FBgn0000052 FBtr0079220; FBpp0078851; FBgn0000052 FBtr0079221; FBpp0078852; FBgn0000052 |
GeneIDi | 33847 |
KEGGi | dme:Dmel_CG9127 |
Organism-specific databases
CTDi | 5198 |
FlyBasei | FBgn0000052, Pfas |
Phylogenomic databases
eggNOGi | KOG1907, Eukaryota |
GeneTreei | ENSGT00390000007600 |
HOGENOMi | CLU_001031_0_0_1 |
InParanoidi | P35421 |
OMAi | LSANWMW |
OrthoDBi | 38224at2759 |
PhylomeDBi | P35421 |
Enzyme and pathway databases
UniPathwayi | UPA00074;UER00128 |
Reactomei | R-DME-73817, Purine ribonucleoside monophosphate biosynthesis |
Miscellaneous databases
BioGRID-ORCSi | 33847, 0 hits in 1 CRISPR screen |
GenomeRNAii | 33847 |
PROi | PR:P35421 |
Gene expression databases
Bgeei | FBgn0000052, Expressed in arthropod fat body and 52 other tissues |
Genevisiblei | P35421, DM |
Family and domain databases
Gene3Di | 3.30.1330.10, 2 hits 3.40.50.880, 1 hit 3.90.650.10, 2 hits |
HAMAPi | MF_00419, PurL_1, 1 hit |
InterProi | View protein in InterPro IPR029062, Class_I_gatase-like IPR040707, FGAR-AT_N IPR017926, GATASE IPR010073, PurL_large IPR041609, PurL_linker IPR010918, PurM-like_C_dom IPR036676, PurM-like_C_sf IPR016188, PurM-like_N IPR036921, PurM-like_N_sf IPR036604, PurS-like_sf |
Pfami | View protein in Pfam PF00586, AIRS, 1 hit PF02769, AIRS_C, 2 hits PF18072, FGAR-AT_linker, 1 hit PF18076, FGAR-AT_N, 1 hit |
SUPFAMi | SSF52317, SSF52317, 1 hit SSF55326, SSF55326, 2 hits SSF56042, SSF56042, 2 hits SSF82697, SSF82697, 1 hit |
TIGRFAMsi | TIGR01735, FGAM_synt, 1 hit |
PROSITEi | View protein in PROSITE PS51273, GATASE_TYPE_1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PUR4_DROME | |
Accessioni | P35421Primary (citable) accession number: P35421 Secondary accession number(s): A4V099, Q6NNY9, Q9VMI7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | January 11, 2001 | |
Last modified: | December 2, 2020 | |
This is version 167 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families