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Protein

Prostaglandin E2 receptor EP4 subtype

Gene

PTGER4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.

Caution

Was originally designated as the EP2 subtype.Curated

GO - Molecular functioni

  • prostaglandin E receptor activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-HSA-391908 Prostanoid ligand receptors
R-HSA-418555 G alpha (s) signalling events
SIGNORiP35408

Names & Taxonomyi

Protein namesi
Recommended name:
Prostaglandin E2 receptor EP4 subtype
Short name:
PGE receptor EP4 subtype
Short name:
PGE2 receptor EP4 subtype
Alternative name(s):
Prostanoid EP4 receptor
Gene namesi
Name:PTGER4
Synonyms:PTGER2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000171522.5
HGNCiHGNC:9596 PTGER4
MIMi601586 gene
neXtProtiNX_P35408

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 19ExtracellularSequence analysisAdd BLAST19
Transmembranei20 – 43Helical; Name=1Sequence analysisAdd BLAST24
Topological domaini44 – 55CytoplasmicSequence analysisAdd BLAST12
Transmembranei56 – 79Helical; Name=2Sequence analysisAdd BLAST24
Topological domaini80 – 96ExtracellularSequence analysisAdd BLAST17
Transmembranei97 – 115Helical; Name=3Sequence analysisAdd BLAST19
Topological domaini116 – 135CytoplasmicSequence analysisAdd BLAST20
Transmembranei136 – 160Helical; Name=4Sequence analysisAdd BLAST25
Topological domaini161 – 184ExtracellularSequence analysisAdd BLAST24
Transmembranei185 – 211Helical; Name=5Sequence analysisAdd BLAST27
Topological domaini212 – 267CytoplasmicSequence analysisAdd BLAST56
Transmembranei268 – 295Helical; Name=6Sequence analysisAdd BLAST28
Topological domaini296 – 312ExtracellularSequence analysisAdd BLAST17
Transmembranei313 – 332Helical; Name=7Sequence analysisAdd BLAST20
Topological domaini333 – 488CytoplasmicSequence analysisAdd BLAST156

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi5734
OpenTargetsiENSG00000171522
PharmGKBiPA289

Chemistry databases

ChEMBLiCHEMBL1836
DrugBankiDB00917 Dinoprostone
DB00929 Misoprostol
GuidetoPHARMACOLOGYi343

Polymorphism and mutation databases

BioMutaiPTGER4
DMDMi548476

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000700641 – 488Prostaglandin E2 receptor EP4 subtypeAdd BLAST488

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi7N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi92 ↔ 170PROSITE-ProRule annotation
Modified residuei374Phosphoserine1 Publication1
Modified residuei377Phosphoserine1 Publication1
Modified residuei379Phosphoserine1 Publication1
Modified residuei382Phosphoserine1 Publication1

Post-translational modificationi

Phosphorylation mediates agonist-mediated desensitization by promoting cytoplasmic retention.2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP35408
PaxDbiP35408
PeptideAtlasiP35408
PRIDEiP35408
ProteomicsDBi55059

PTM databases

iPTMnetiP35408
PhosphoSitePlusiP35408

Expressioni

Tissue specificityi

High in intestine and in peripheral blood mononuclear cells; low in lung, kidney, thymus, uterus, vasculature and brain. Not found in liver, heart, retina oe skeletal muscle.

Gene expression databases

BgeeiENSG00000171522 Expressed in 211 organ(s), highest expression level in metanephric glomerulus
CleanExiHS_PTGER2
HS_PTGER4
GenevisibleiP35408 HS

Organism-specific databases

HPAiHPA011226
HPA012756

Interactioni

Subunit structurei

Interacts with FEM1A.1 Publication

Protein-protein interaction databases

BioGridi111706, 5 interactors
DIPiDIP-61099N
IntActiP35408, 2 interactors
STRINGi9606.ENSP00000302846

Chemistry databases

BindingDBiP35408

Structurei

3D structure databases

ProteinModelPortaliP35408
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi230 – 234Poly-Ala5

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656 Eukaryota
ENOG410XRW9 LUCA
GeneTreeiENSGT00760000119188
HOGENOMiHOG000251595
HOVERGENiHBG053556
InParanoidiP35408
KOiK04261
OMAiHSVSVTM
OrthoDBiEOG091G060C
PhylomeDBiP35408
TreeFamiTF324982

Family and domain databases

CDDicd15142 7tmA_PGE2_EP4, 1 hit
InterProiView protein in InterPro
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
IPR001758 Prost_EP4_rcpt
IPR008365 Prostanoid_rcpt
IPR001244 Prostglndn_DP_rcpt
PANTHERiPTHR11866 PTHR11866, 1 hit
PTHR11866:SF6 PTHR11866:SF6, 1 hit
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PRINTSiPR00237 GPCRRHODOPSN
PR00428 PROSTAGLNDNR
PR01788 PROSTANOIDR
PR00586 PRSTNOIDEP4R
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit

Sequencei

Sequence statusi: Complete.

P35408-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSTPGVNSSA SLSPDRLNSP VTIPAVMFIF GVVGNLVAIV VLCKSRKEQK
60 70 80 90 100
ETTFYTLVCG LAVTDLLGTL LVSPVTIATY MKGQWPGGQP LCEYSTFILL
110 120 130 140 150
FFSLSGLSII CAMSVERYLA INHAYFYSHY VDKRLAGLTL FAVYASNVLF
160 170 180 190 200
CALPNMGLGS SRLQYPDTWC FIDWTTNVTA HAAYSYMYAG FSSFLILATV
210 220 230 240 250
LCNVLVCGAL LRMHRQFMRR TSLGTEQHHA AAAASVASRG HPAASPALPR
260 270 280 290 300
LSDFRRRRSF RRIAGAEIQM VILLIATSLV VLICSIPLVV RVFVNQLYQP
310 320 330 340 350
SLEREVSKNP DLQAIRIASV NPILDPWIYI LLRKTVLSKA IEKIKCLFCR
360 370 380 390 400
IGGSRRERSG QHCSDSQRTS SAMSGHSRSF ISRELKEISS TSQTLLPDLS
410 420 430 440 450
LPDLSENGLG GRNLLPGVPG MGLAQEDTTS LRTLRISETS DSSQGQDSES
460 470 480
VLLVDEAGGS GRAGPAPKGS SLQVTFPSET LNLSEKCI
Length:488
Mass (Da):53,119
Last modified:June 1, 1994 - v1
Checksum:iD028478CD72C85EE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti464 – 466GPA → WAC in AAA36438 (PubMed:8250933).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L28175 mRNA Translation: AAA36434.1
L25124 mRNA Translation: AAA36438.1
X97873, X97874 Genomic DNA Translation: CAA66463.1
D28472 mRNA Translation: BAA05834.1
AY429109 mRNA Translation: AAR07904.1
DQ400918 Genomic DNA Translation: ABD48960.1
BC101534 mRNA Translation: AAI01535.1
BC113523 mRNA Translation: AAI13524.1
CCDSiCCDS3930.1
PIRiA53572
RefSeqiNP_000949.1, NM_000958.2
UniGeneiHs.199248

Genome annotation databases

EnsembliENST00000302472; ENSP00000302846; ENSG00000171522
GeneIDi5734
KEGGihsa:5734
UCSCiuc003jlz.5 human

Similar proteinsi

Cross-referencesi

Web resourcesi

SeattleSNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L28175 mRNA Translation: AAA36434.1
L25124 mRNA Translation: AAA36438.1
X97873, X97874 Genomic DNA Translation: CAA66463.1
D28472 mRNA Translation: BAA05834.1
AY429109 mRNA Translation: AAR07904.1
DQ400918 Genomic DNA Translation: ABD48960.1
BC101534 mRNA Translation: AAI01535.1
BC113523 mRNA Translation: AAI13524.1
CCDSiCCDS3930.1
PIRiA53572
RefSeqiNP_000949.1, NM_000958.2
UniGeneiHs.199248

3D structure databases

ProteinModelPortaliP35408
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111706, 5 interactors
DIPiDIP-61099N
IntActiP35408, 2 interactors
STRINGi9606.ENSP00000302846

Chemistry databases

BindingDBiP35408
ChEMBLiCHEMBL1836
DrugBankiDB00917 Dinoprostone
DB00929 Misoprostol
GuidetoPHARMACOLOGYi343

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiP35408
PhosphoSitePlusiP35408

Polymorphism and mutation databases

BioMutaiPTGER4
DMDMi548476

Proteomic databases

MaxQBiP35408
PaxDbiP35408
PeptideAtlasiP35408
PRIDEiP35408
ProteomicsDBi55059

Protocols and materials databases

DNASUi5734
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000302472; ENSP00000302846; ENSG00000171522
GeneIDi5734
KEGGihsa:5734
UCSCiuc003jlz.5 human

Organism-specific databases

CTDi5734
DisGeNETi5734
EuPathDBiHostDB:ENSG00000171522.5
GeneCardsiPTGER4
H-InvDBiHIX0032033
HGNCiHGNC:9596 PTGER4
HPAiHPA011226
HPA012756
MIMi601586 gene
neXtProtiNX_P35408
OpenTargetsiENSG00000171522
PharmGKBiPA289
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3656 Eukaryota
ENOG410XRW9 LUCA
GeneTreeiENSGT00760000119188
HOGENOMiHOG000251595
HOVERGENiHBG053556
InParanoidiP35408
KOiK04261
OMAiHSVSVTM
OrthoDBiEOG091G060C
PhylomeDBiP35408
TreeFamiTF324982

Enzyme and pathway databases

ReactomeiR-HSA-391908 Prostanoid ligand receptors
R-HSA-418555 G alpha (s) signalling events
SIGNORiP35408

Miscellaneous databases

GeneWikiiEP4_receptor
GenomeRNAii5734
PROiPR:P35408
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000171522 Expressed in 211 organ(s), highest expression level in metanephric glomerulus
CleanExiHS_PTGER2
HS_PTGER4
GenevisibleiP35408 HS

Family and domain databases

CDDicd15142 7tmA_PGE2_EP4, 1 hit
InterProiView protein in InterPro
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
IPR001758 Prost_EP4_rcpt
IPR008365 Prostanoid_rcpt
IPR001244 Prostglndn_DP_rcpt
PANTHERiPTHR11866 PTHR11866, 1 hit
PTHR11866:SF6 PTHR11866:SF6, 1 hit
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PRINTSiPR00237 GPCRRHODOPSN
PR00428 PROSTAGLNDNR
PR01788 PROSTANOIDR
PR00586 PRSTNOIDEP4R
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPE2R4_HUMAN
AccessioniPrimary (citable) accession number: P35408
Secondary accession number(s): Q3MJ87
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: September 12, 2018
This is version 170 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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