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Protein

Polygalacturonase

Gene
N/A
Organism
Actinidia deliciosa (Kiwi)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Acts in concert with the pectinesterase, in the ripening process. Is involved in cell wall metabolism, specifically in polyuronide degradation.

Catalytic activityi

Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei283Proton donorPROSITE-ProRule annotation1
Active sitei306PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCell wall biogenesis/degradation, Fruit ripening

Protein family/group databases

CAZyiGH28 Glycoside Hydrolase Family 28

Names & Taxonomyi

Protein namesi
Recommended name:
Polygalacturonase (EC:3.2.1.15)
Short name:
PG
Alternative name(s):
Pectinase
OrganismiActinidia deliciosa (Kiwi)
Taxonomic identifieri3627 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridsEricalesActinidiaceaeActinidia

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_000002479828 – 467PolygalacturonaseAdd BLAST440

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi290N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiP35336

Expressioni

Developmental stagei

In ripening fruit.

Structurei

3D structure databases

ProteinModelPortaliP35336
SMRiP35336
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 28 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.160.20.10, 1 hit
InterProiView protein in InterPro
IPR000743 Glyco_hydro_28
IPR006626 PbH1
IPR012334 Pectin_lyas_fold
IPR011050 Pectin_lyase_fold/virulence
PfamiView protein in Pfam
PF00295 Glyco_hydro_28, 1 hit
SMARTiView protein in SMART
SM00710 PbH1, 4 hits
SUPFAMiSSF51126 SSF51126, 1 hit
PROSITEiView protein in PROSITE
PS00502 POLYGALACTURONASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35336-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MALQRRFFQF VIITLLIPSF ILGYTSAVHE DPPHDYHLEE YGYDFKAYPS
60 70 80 90 100
YITTIGDNDF GSSMSHENGI FGLRKVDYGM DRVLDASKTV NVDDFGAKGD
110 120 130 140 150
GRDDTKAFEK AWKAACSSTS SAVLLVPKKN YLVRPISFSG PCKSGLTMQI
160 170 180 190 200
YGTIEASDDR SDYRKDGRHW LVFDSVQNLR VEGGGTINGN GKIWWQNSCK
210 220 230 240 250
TNKALPCKDA PTALTFYKSK HVIVKNLKIE NAQQIHVSFD NCVNVQASNL
260 270 280 290 300
MVTAPENSPN TDGIHVTGTQ NIHISSCVIG TGDDCISIVN GSRKVRVNDI
310 320 330 340 350
TCGPGHGISI GSLGYGNSEA HVSDVVVNGA KLCGTTNGVR IKTWQGGSGS
360 370 380 390 400
ASNIKFQNVE MHNVENPIII DQNYCDQDKP CQEQSSAVQV KNVVYQNIKG
410 420 430 440 450
TCASNVAITF DCSKRFPCQG IVLEDVDLEI EGGAAAKALC NNVELSETGV
460
VSPHCQEEGG EEEEEAS
Length:467
Mass (Da):50,776
Last modified:June 1, 1994 - v1
Checksum:i5A9A61483C028B7A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12019 Genomic DNA Translation: AAC14453.1

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12019 Genomic DNA Translation: AAC14453.1

3D structure databases

ProteinModelPortaliP35336
SMRiP35336
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH28 Glycoside Hydrolase Family 28

Proteomic databases

PRIDEiP35336

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.160.20.10, 1 hit
InterProiView protein in InterPro
IPR000743 Glyco_hydro_28
IPR006626 PbH1
IPR012334 Pectin_lyas_fold
IPR011050 Pectin_lyase_fold/virulence
PfamiView protein in Pfam
PF00295 Glyco_hydro_28, 1 hit
SMARTiView protein in SMART
SM00710 PbH1, 4 hits
SUPFAMiSSF51126 SSF51126, 1 hit
PROSITEiView protein in PROSITE
PS00502 POLYGALACTURONASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPGLR_ACTDE
AccessioniPrimary (citable) accession number: P35336
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: May 23, 2018
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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