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UniProtKB - P35285 (RB22A_MOUSE)
Protein
Ras-related protein Rab-22A
Gene
Rab22a
Organism
Mus musculus (Mouse)
Status
Functioni
Plays a role in endocytosis and intracellular protein transport (PubMed:19759177, PubMed:27718357).
Mediates trafficking of TF from early endosomes to recycling endosomes. Required for NGF-mediated endocytosis of NTRK1, and subsequent neurite outgrowth. Binds GTP and GDP and has low GTPase activity. Alternates between a GTP-bound active form and a GDP-bound inactive form (By similarity).
By similarity2 PublicationsRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 12 – 20 | GTP | 9 | |
Nucleotide bindingi | 60 – 64 | GTP | 5 | |
Nucleotide bindingi | 118 – 121 | GTP | 4 | |
Nucleotide bindingi | 148 – 150 | GTP | 3 |
GO - Molecular functioni
- GDP binding Source: UniProtKB
- GTPase activity Source: UniProtKB
- GTP binding Source: UniProtKB
GO - Biological processi
- endocytosis Source: MGI
- endosome organization Source: Ensembl
- intracellular protein transport Source: GO_Central
- regulation of vesicle size Source: MGI
Keywordsi
Biological process | Endocytosis, Protein transport, Transport |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-8873719, RAB geranylgeranylation |
Names & Taxonomyi
Protein namesi | Recommended name: Ras-related protein Rab-22AShort name: Rab-22 Alternative name(s): Rab-14 |
Gene namesi | Name:Rab22a Synonyms:Rab22 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:105072, Rab22a |
VEuPathDBi | HostDB:ENSMUSG00000027519 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Lipid-anchor Curated
Endosome
- Endosome membrane 1 Publication; Lipid-anchor Curated
- Early endosome 2 Publications
- Late endosome By similarity
Other locations
- ruffle By similarity
- Cytoplasmic vesicle 1 Publication
- phagosome By similarity
- phagosome membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated
Note: Recruited to phagosomes containing S.aureus or M.tuberculosis.By similarity
Cytoskeleton
- actin cytoskeleton Source: MGI
Endosome
- early endosome Source: MGI
- endosome membrane Source: UniProtKB-SubCell
- late endosome Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- endomembrane system Source: GO_Central
- phagocytic vesicle Source: UniProtKB
- phagocytic vesicle membrane Source: UniProtKB-SubCell
- ruffle Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell membrane, Cell projection, Cytoplasmic vesicle, Endosome, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 64 | Q → L: Constitutively active. Disrupts general recycling of receptors in embryonic fibroblasts. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000121210 | 1 – 194 | Ras-related protein Rab-22AAdd BLAST | 194 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 193 | S-geranylgeranyl cysteineBy similarity | 1 | |
Lipidationi | 194 | S-geranylgeranyl cysteineBy similarity | 1 |
Keywords - PTMi
Lipoprotein, PrenylationProteomic databases
EPDi | P35285 |
PaxDbi | P35285 |
PeptideAtlasi | P35285 |
PRIDEi | P35285 |
ProteomicsDBi | 300246 |
PTM databases
iPTMneti | P35285 |
PhosphoSitePlusi | P35285 |
Expressioni
Tissue specificityi
Detected in brain and heart, and at lower levels in lung and spleen.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000027519, Expressed in humerus cartilage element and 299 other tissues |
ExpressionAtlasi | P35285, baseline and differential |
Genevisiblei | P35285, MM |
Interactioni
Subunit structurei
Protein-protein interaction databases
BioGRIDi | 202539, 1 interactor |
DIPi | DIP-60516N |
IntActi | P35285, 33 interactors |
STRINGi | 10090.ENSMUSP00000029024 |
Miscellaneous databases
RNActi | P35285, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P35285 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P35285 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 170 – 194 | DisorderedSequence analysisAdd BLAST | 25 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 34 – 42 | Effector regionBy similarity | 9 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0092, Eukaryota |
GeneTreei | ENSGT00940000157009 |
HOGENOMi | CLU_041217_10_2_1 |
InParanoidi | P35285 |
OMAi | DGIDCAE |
OrthoDBi | 1340129at2759 |
PhylomeDBi | P35285 |
TreeFami | TF331262 |
Family and domain databases
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SMARTi | View protein in SMART SM00174, RHO, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51419, RAB, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
P35285-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MALRELKVCL LGDTGVGKSS IVWRFVEDSF DPNINPTIGA SFMTKTVQYQ
60 70 80 90 100
NELHKFLIWD TAGQERFRAL APMYYRGSAA AIIVYDITKE ETFSTLKNWV
110 120 130 140 150
RELRQHGPPS IVVAIAGNKC DLTDVREVME RDAKDYADSI HAIFVETSAK
160 170 180 190
NAININELFI EISRRIPSTD ANPASGGKGF KLRRQPSEPK RSCC
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA2ARZ7 | A2ARZ7_MOUSE | Ras-related protein Rab-22A | Rab22a | 187 | Annotation score: | ||
D6RFT6 | D6RFT6_MOUSE | Ras-related protein Rab-22A | Rab22a | 68 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 54 | H → R in AAK14828 (PubMed:1555775).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ311124 mRNA Translation: CAC41378.1 AK031667 mRNA Translation: BAC27501.1 AK146594 mRNA Translation: BAE27289.1 AK150617 mRNA Translation: BAE29707.1 BC006596 mRNA Translation: AAH06596.1 M79304 mRNA Translation: AAK14828.1 |
CCDSi | CCDS38352.1 |
PIRi | JH0644 |
RefSeqi | NP_077756.2, NM_024436.3 |
Genome annotation databases
Ensembli | ENSMUST00000029024; ENSMUSP00000029024; ENSMUSG00000027519 |
GeneIDi | 19334 |
KEGGi | mmu:19334 |
UCSCi | uc008oed.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ311124 mRNA Translation: CAC41378.1 AK031667 mRNA Translation: BAC27501.1 AK146594 mRNA Translation: BAE27289.1 AK150617 mRNA Translation: BAE29707.1 BC006596 mRNA Translation: AAH06596.1 M79304 mRNA Translation: AAK14828.1 |
CCDSi | CCDS38352.1 |
PIRi | JH0644 |
RefSeqi | NP_077756.2, NM_024436.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1YVD | X-ray | 1.93 | A | 3-169 | [»] | |
1Z0J | X-ray | 1.32 | A | 2-169 | [»] | |
SMRi | P35285 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 202539, 1 interactor |
DIPi | DIP-60516N |
IntActi | P35285, 33 interactors |
STRINGi | 10090.ENSMUSP00000029024 |
PTM databases
iPTMneti | P35285 |
PhosphoSitePlusi | P35285 |
Proteomic databases
EPDi | P35285 |
PaxDbi | P35285 |
PeptideAtlasi | P35285 |
PRIDEi | P35285 |
ProteomicsDBi | 300246 |
Protocols and materials databases
Antibodypediai | 29093, 159 antibodies from 30 providers |
DNASUi | 19334 |
Genome annotation databases
Ensembli | ENSMUST00000029024; ENSMUSP00000029024; ENSMUSG00000027519 |
GeneIDi | 19334 |
KEGGi | mmu:19334 |
UCSCi | uc008oed.1, mouse |
Organism-specific databases
CTDi | 57403 |
MGIi | MGI:105072, Rab22a |
VEuPathDBi | HostDB:ENSMUSG00000027519 |
Phylogenomic databases
eggNOGi | KOG0092, Eukaryota |
GeneTreei | ENSGT00940000157009 |
HOGENOMi | CLU_041217_10_2_1 |
InParanoidi | P35285 |
OMAi | DGIDCAE |
OrthoDBi | 1340129at2759 |
PhylomeDBi | P35285 |
TreeFami | TF331262 |
Enzyme and pathway databases
Reactomei | R-MMU-8873719, RAB geranylgeranylation |
Miscellaneous databases
BioGRID-ORCSi | 19334, 1 hit in 64 CRISPR screens |
ChiTaRSi | Rab22a, mouse |
EvolutionaryTracei | P35285 |
PROi | PR:P35285 |
RNActi | P35285, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000027519, Expressed in humerus cartilage element and 299 other tissues |
ExpressionAtlasi | P35285, baseline and differential |
Genevisiblei | P35285, MM |
Family and domain databases
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SMARTi | View protein in SMART SM00174, RHO, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51419, RAB, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RB22A_MOUSE | |
Accessioni | P35285Primary (citable) accession number: P35285 Secondary accession number(s): Q3UCA7, Q91VD4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | April 26, 2004 | |
Last modified: | February 23, 2022 | |
This is version 179 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families