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Protein

Ras-related protein Rab-12

Gene

Rab12

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab may play a role in protein transport from recycling endosomes to lysosomes regulating, for instance, the degradation of the transferrin receptor. Involved in autophagy (By similarity).By similarity

Activity regulationi

Rab activation is generally mediated by a guanine exchange factor (GEF), while inactivation through hydrolysis of bound GTP is catalyzed by a GTPase activating protein (GAP). That Rab is activated by DENND3, a guanine exchange factor (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi48 – 56GTPBy similarity9
Nucleotide bindingi96 – 100GTPBy similarity5
Nucleotide bindingi154 – 158GTPBy similarity5
Nucleotide bindingi186 – 187GTPBy similarity2

GO - Molecular functioni

  • GDP binding Source: UniProtKB
  • GTPase activity Source: GO_Central
  • GTP binding Source: RGD

GO - Biological processi

Keywordsi

Biological processAutophagy, Protein transport, Transport
LigandGTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-12
Gene namesi
Name:Rab12
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3527 Rab12

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasmic vesicle, Endosome, Golgi apparatus, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001211811 – 243Ras-related protein Rab-12Add BLAST243

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei20PhosphoserineCombined sources1
Modified residuei24PhosphoserineBy similarity1
Modified residuei105PhosphoserineBy similarity1
Lipidationi242S-geranylgeranyl cysteineBy similarity1
Lipidationi243S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

Acetylation, Lipoprotein, Phosphoprotein, Prenylation

Proteomic databases

PaxDbiP35284
PRIDEiP35284

PTM databases

iPTMnetiP35284
PhosphoSitePlusiP35284

Expressioni

Tissue specificityi

Highest levels in skeletal and cardiac muscle. Also found in comparable amounts in brain, spinal cord and lung. Also detected in testis where it is expressed by Sertoli cells of the seminiferous tubules (at protein level).1 Publication

Interactioni

Subunit structurei

Interacts with RABIF and OPTN.By similarity

Protein-protein interaction databases

DIPiDIP-36918N
IntActiP35284, 2 interactors
STRINGi10116.ENSRNOP00000059602

Structurei

3D structure databases

SMRiP35284
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi70 – 78Effector regionBy similarity9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0078 Eukaryota
ENOG410XPUI LUCA
HOVERGENiHBG009351
InParanoidiP35284
KOiK07907

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51419 RAB, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P35284-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDPSAALHRR PAGGGLGAVS PALSGGQARR RKQPPRPADF KLQVIIIGSR
60 70 80 90 100
GVGKTSLMER FTDDTFCEAC KSTVGVDFKI KTVELRGKKI RLQIWDTAGQ
110 120 130 140 150
ERFNSITSAY YRSAKGIILV YDITKKETFD DLPKWMKMID KYASEDAELL
160 170 180 190 200
LVGNKLDCET DREISRQQGE KFAQQITGMR FCEASAKDNF NVDEIFLKLV
210 220 230 240
DDILKKMPLD VLRSELSNSI LSLQPEPEIP PELPPPRPHV RCC
Length:243
Mass (Da):27,271
Last modified:February 6, 2013 - v2
Checksum:i237A9421E6784584
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D4A376D4A376_RAT
Ras-related protein Rab-12
Rab12 LOC100362985
245Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti152V → A in AAA41992 (PubMed:1313420).Curated1
Sequence conflicti181F → L in AAA41992 (PubMed:1313420).Curated1
Sequence conflicti182C → S in AAA41992 (PubMed:1313420).Curated1
Sequence conflicti184A → P in AAA41992 (PubMed:1313420).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M83676 mRNA Translation: AAA41992.1
PIRiC42148
RefSeqiNP_037149.1, NM_013017.1
UniGeneiRn.11021

Genome annotation databases

GeneIDi25530
KEGGirno:25530

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M83676 mRNA Translation: AAA41992.1
PIRiC42148
RefSeqiNP_037149.1, NM_013017.1
UniGeneiRn.11021

3D structure databases

SMRiP35284
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-36918N
IntActiP35284, 2 interactors
STRINGi10116.ENSRNOP00000059602

PTM databases

iPTMnetiP35284
PhosphoSitePlusiP35284

Proteomic databases

PaxDbiP35284
PRIDEiP35284

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi25530
KEGGirno:25530

Organism-specific databases

CTDi201475
RGDi3527 Rab12

Phylogenomic databases

eggNOGiKOG0078 Eukaryota
ENOG410XPUI LUCA
HOVERGENiHBG009351
InParanoidiP35284
KOiK07907

Miscellaneous databases

PROiPR:P35284

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51419 RAB, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRAB12_RAT
AccessioniPrimary (citable) accession number: P35284
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 6, 2013
Last modified: November 7, 2018
This is version 135 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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