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UniProtKB - P35269 (T2FA_HUMAN)

Entry version 211 (23 Feb 2022)
Sequence version 2 (13 Apr 2004)
Previous versions | rss
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Protein

General transcription factor IIF subunit 1

Gene

GTF2F1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.

1 Publication

Caution

Was reported to have a protein kinase activity and to autophosphorylates on Ser-385 and Thr-389.1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

Pathway Commons web resource for biological pathway data

More...PathwayCommonsi		P35269

Reactome - a knowledgebase of biological pathways and processes

More...Reactomei		R-HSA-112382, Formation of RNA Pol II elongation complex
R-HSA-113418, Formation of the Early Elongation Complex
R-HSA-167152, Formation of HIV elongation complex in the absence of HIV Tat
R-HSA-167158, Formation of the HIV-1 Early Elongation Complex
R-HSA-167160, RNA Pol II CTD phosphorylation and interaction with CE during HIV infection
R-HSA-167161, HIV Transcription Initiation
R-HSA-167162, RNA Polymerase II HIV Promoter Escape
R-HSA-167172, Transcription of the HIV genome
R-HSA-167200, Formation of HIV-1 elongation complex containing HIV-1 Tat
R-HSA-167238, Pausing and recovery of Tat-mediated HIV elongation
R-HSA-167242, Abortive elongation of HIV-1 transcript in the absence of Tat
R-HSA-167243, Tat-mediated HIV elongation arrest and recovery
R-HSA-167246, Tat-mediated elongation of the HIV-1 transcript
R-HSA-167287, HIV elongation arrest and recovery
R-HSA-167290, Pausing and recovery of HIV elongation
R-HSA-168325, Viral Messenger RNA Synthesis
R-HSA-674695, RNA Polymerase II Pre-transcription Events
R-HSA-6796648, TP53 Regulates Transcription of DNA Repair Genes
R-HSA-6803529, FGFR2 alternative splicing
R-HSA-6807505, RNA polymerase II transcribes snRNA genes
R-HSA-72086, mRNA Capping
R-HSA-72163, mRNA Splicing - Major Pathway
R-HSA-72165, mRNA Splicing - Minor Pathway
R-HSA-72203, Processing of Capped Intron-Containing Pre-mRNA
R-HSA-73776, RNA Polymerase II Promoter Escape
R-HSA-73779, RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-HSA-75953, RNA Polymerase II Transcription Initiation
R-HSA-75955, RNA Polymerase II Transcription Elongation
R-HSA-76042, RNA Polymerase II Transcription Initiation And Promoter Clearance
R-HSA-77075, RNA Pol II CTD phosphorylation and interaction with CE
R-HSA-8851708, Signaling by FGFR2 IIIa TM
R-HSA-9018519, Estrogen-dependent gene expression

SignaLink: a signaling pathway resource with multi-layered regulatory networks

More...SignaLinki		P35269

SIGNOR Signaling Network Open Resource

More...SIGNORi		P35269

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
General transcription factor IIF subunit 1
Alternative name(s):
General transcription factor IIF 74 kDa subunit
Transcription initiation factor IIF subunit alpha
Short name:
TFIIF-alpha
Transcription initiation factor RAP74
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:GTF2F1
Synonyms:RAP74
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 19

Organism-specific databases

Human Gene Nomenclature Database

More...HGNCi		HGNC:4652, GTF2F1

Online Mendelian Inheritance in Man (OMIM)

More...MIMi		189968, gene

neXtProt; the human protein knowledge platform

More...neXtProti		NX_P35269

Eukaryotic Pathogen, Vector and Host Database Resources

More...VEuPathDBi		HostDB:ENSG00000125651

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi385S → A: Eliminates putative kinase activity; when associated with A-389. 1 Publication1
Mutagenesisi389T → A: Eliminates putative kinase activity; when associated with A-385. 1 Publication1

Organism-specific databases

DisGeNET

More...DisGeNETi		2962

Open Targets

More...OpenTargetsi		ENSG00000125651

The Pharmacogenetics and Pharmacogenomics Knowledge Base

More...PharmGKBi		PA29038

Miscellaneous databases

Pharos NIH Druggable Genome Knowledgebase

More...Pharosi		P35269, Tbio

Genetic variation databases

BioMuta curated single-nucleotide variation and disease association database

More...BioMutai		GTF2F1

Domain mapping of disease mutations (DMDM)

More...DMDMi		46397744

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM / Processing</a> section indicates that the initiator methionine is cleaved from the mature protein.<p><a href='/help/init_met' target='_top'>More...</a></p>Initiator methionineiRemovedCombined sources
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002112312 – 517General transcription factor IIF subunit 1Add BLAST516

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei2N-acetylalanineCombined sources1
Modified residuei156PhosphothreonineCombined sources1
Modified residuei217PhosphoserineCombined sources1
Modified residuei218PhosphoserineCombined sources1
Modified residuei221PhosphoserineCombined sources1
Modified residuei224PhosphoserineCombined sources1
Modified residuei331PhosphothreonineCombined sources1
Modified residuei377PhosphoserineCombined sources1
Modified residuei380PhosphoserineCombined sources1
Modified residuei381PhosphoserineCombined sources1
Modified residuei385PhosphoserineCombined sources1 Publication1
Modified residuei389PhosphothreonineCombined sources1 Publication1
Modified residuei391PhosphoserineCombined sources1
Modified residuei407N6-acetyllysineCombined sources1
Modified residuei431PhosphoserineCombined sources1
Modified residuei433PhosphoserineCombined sources1
Modified residuei436PhosphoserineCombined sources1
Modified residuei437PhosphothreonineCombined sources1
Modified residuei446PhosphothreonineCombined sources1
Modified residuei449PhosphoserineCombined sources1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Phosphorylated on Ser and other residues by TAF1 and casein kinase II-like kinases.2 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

Encyclopedia of Proteome Dynamics

More...EPDi		P35269

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		P35269

MassIVE - Mass Spectrometry Interactive Virtual Environment

More...MassIVEi		P35269

MaxQB - The MaxQuant DataBase

More...MaxQBi		P35269

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P35269

PeptideAtlas

More...PeptideAtlasi		P35269

PRoteomics IDEntifications database

More...PRIDEi		P35269

ProteomicsDB: a multi-organism proteome resource

More...ProteomicsDBi		55019

Consortium for Top Down Proteomics

More...TopDownProteomicsi		P35269

PTM databases

GlyGen: Computational and Informatics Resources for Glycoscience

More...GlyGeni		P35269, 1 site, 1 O-linked glycan (1 site)

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		P35269

MetOSite database of methionine sulfoxide sites

More...MetOSitei		P35269

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...PhosphoSitePlusi		P35269

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

Up-regulated in response to enterovirus 71 (EV71) infection.1 Publication

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...Bgeei		ENSG00000125651, Expressed in testis and 124 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...ExpressionAtlasi		P35269, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...Genevisiblei		P35269, HS

Organism-specific databases

Human Protein Atlas

More...HPAi		ENSG00000125651, Low tissue specificity

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Heterodimer of an alpha and a beta subunit.

Interacts with GTF2F2, CTDP1, TAF6/TAFII80 and URI1.

Interacts with GTF2B (via C-terminus and preferentially via acetylated form); this interaction prevents binding of GTF2B to GTF2F2 (PubMed:8662660, PubMed:12931194).

6 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Hide details

GO - Molecular functioni

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		109217, 156 interactors

ComplexPortal: manually curated resource of macromolecular complexes

More...ComplexPortali		CPX-79, Transcription factor TFIIF complex

CORUM comprehensive resource of mammalian protein complexes

More...CORUMi		P35269

Database of interacting proteins

More...DIPi		DIP-677N

Protein interaction database and analysis system

More...IntActi		P35269, 59 interactors

Molecular INTeraction database

More...MINTi		P35269

STRING: functional protein association networks

More...STRINGi		9606.ENSP00000377969

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		P35269, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1517
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

Biological Magnetic Resonance Data Bank

More...BMRBi		P35269

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P35269

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...EvolutionaryTracei		P35269

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni178 – 466DisorderedSequence analysisAdd BLAST289

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular type of amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi179 – 214Basic and acidic residuesSequence analysisAdd BLAST36
Compositional biasi273 – 287Polar residuesSequence analysisAdd BLAST15
Compositional biasi288 – 305Basic and acidic residuesSequence analysisAdd BLAST18
Compositional biasi306 – 320Acidic residuesSequence analysisAdd BLAST15
Compositional biasi321 – 345Basic and acidic residuesSequence analysisAdd BLAST25
Compositional biasi380 – 405Polar residuesSequence analysisAdd BLAST26
Compositional biasi429 – 453Polar residuesSequence analysisAdd BLAST25

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the TFIIF alpha subunit family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		KOG2393, Eukaryota

Ensembl GeneTree

More...GeneTreei		ENSGT00440000038032

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_027572_2_0_1

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P35269

Identification of Orthologs from Complete Genome Data

More...OMAi		AFYVFTH

Database of Orthologous Groups

More...OrthoDBi		893557at2759

Database for complete collections of gene phylogenies

More...PhylomeDBi		P35269

TreeFam database of animal gene trees

More...TreeFami		TF313850

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		1.10.10.10, 1 hit

Intrinsically Disordered proteins with Extensive Annotations and Literature

More...IDEALi		IID00369

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR008851, TFIIF-alpha
IPR011039, TFIIF_interaction
IPR036388, WH-like_DNA-bd_sf
IPR036390, WH_DNA-bd_sf

The PANTHER Classification System

More...PANTHERi		PTHR13011, PTHR13011, 1 hit

Pfam protein domain database

More...Pfami		View protein in Pfam
PF05793, TFIIF_alpha, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF46785, SSF46785, 1 hit
SSF50916, SSF50916, 2 hits

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequence (1+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

P35269-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAALGPSSQN VTEYVVRVPK NTTKKYNIMA FNAADKVNFA TWNQARLERD 
        60         70         80         90        100
LSNKKIYQEE EMPESGAGSE FNRKLREEAR RKKYGIVLKE FRPEDQPWLL 
       110        120        130        140        150
RVNGKSGRKF KGIKKGGVTE NTSYYIFTQC PDGAFEAFPV HNWYNFTPLA 
       160        170        180        190        200
RHRTLTAEEA EEEWERRNKV LNHFSIMQQR RLKDQDQDED EEEKEKRGRR 
       210        220        230        240        250
KASELRIHDL EDDLEMSSDA SDASGEEGGR VPKAKKKAPL AKGGRKKKKK 
       260        270        280        290        300
KGSDDEAFED SDDGDFEGQE VDYMSDGSSS SQEEPESKAK APQQEEGPKG 
       310        320        330        340        350
VDEQSDSSEE SEEEKPPEED KEEEEEKKAP TPQEKKRRKD SSEESDSSEE 
       360        370        380        390        400
SDIDSEASSA LFMAKKKTPP KRERKPSGGS SRGNSRPGTP SAEGGSTSST 
       410        420        430        440        450
LRAAASKLEQ GKRVSEMPAA KRLRLDTGPQ SLSGKSTPQP PSGKTTPNSG 
       460        470        480        490        500
DVQVTEDAVR RYLTRKPMTT KDLLKKFQTK KTGLSSEQTV NVLAQILKRL 
       510 
NPERKMINDK MHFSLKE
Length:517
Mass (Da):58,240
Last modified:April 13, 2004 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i1032B04A36BDC24F
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
M0QXD6M0QXD6_HUMAN
Transcription initiation factor IIF...
GTF2F1
433Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
M0R0R9M0R0R9_HUMAN
Transcription initiation factor IIF...
GTF2F1
381Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
M0R0Z3M0R0Z3_HUMAN
Transcription initiation factor IIF...
GTF2F1
127Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti231V → I in CAA45404 (PubMed:1734284).Curated1
Sequence conflicti361L → F in CAA45408 (PubMed:1734283).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_0390043A → V. Corresponds to variant dbSNP:rs34826931Ensembl.1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
X64037 mRNA Translation: CAA45408.1
X64002 mRNA Translation: CAA45404.1
BT007097 mRNA Translation: AAP35761.1
AK315240 mRNA Translation: BAG37667.1
CH471139 Genomic DNA Translation: EAW69098.1
BC000120 mRNA Translation: AAH00120.1
BC013007 mRNA Translation: AAH13007.1

The Consensus CDS (CCDS) project

More...CCDSi		CCDS12165.1

Protein sequence database of the Protein Information Resource

More...PIRi		S20248

NCBI Reference Sequences

More...RefSeqi		NP_002087.2, NM_002096.2

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...Ensembli		ENST00000394456; ENSP00000377969; ENSG00000125651

Database of genes from NCBI RefSeq genomes

More...GeneIDi		2962

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		hsa:2962

Matched Annotation from NCBI and EMBL-EBI (MANE) - Phase one

More...MANE-Selecti		ENST00000394456.10; ENSP00000377969.3; NM_002096.3; NP_002087.2

UCSC genome browser

More...UCSCi		uc002meq.3, human

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64037 mRNA Translation: CAA45408.1
X64002 mRNA Translation: CAA45404.1
BT007097 mRNA Translation: AAP35761.1
AK315240 mRNA Translation: BAG37667.1
CH471139 Genomic DNA Translation: EAW69098.1
BC000120 mRNA Translation: AAH00120.1
BC013007 mRNA Translation: AAH13007.1
CCDSiCCDS12165.1
PIRiS20248
RefSeqiNP_002087.2, NM_002096.2

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F3UX-ray1.70B/D/F/H2-172[»]
1I27X-ray1.02A449-517[»]
1J2XX-ray2.00A449-517[»]
1NHANMR-A436-517[»]
1ONVNMR-A436-517[»]
2K7LNMR-A451-517[»]
5IY6electron microscopy7.20S1-517[»]
5IY7electron microscopy8.60S1-517[»]
5IY8electron microscopy7.90S1-517[»]
5IY9electron microscopy6.30S1-517[»]
5IYAelectron microscopy5.40S1-517[»]
5IYBelectron microscopy3.90S1-517[»]
5IYCelectron microscopy3.90S1-517[»]
5IYDelectron microscopy3.90S1-517[»]
6O9Lelectron microscopy7.20S1-517[»]
7EDXelectron microscopy4.50S1-517[»]
7EG7electron microscopy6.20S1-517[»]
7EG8electron microscopy7.40S1-517[»]
7EG9electron microscopy3.70S1-517[»]
7EGAelectron microscopy4.10S1-517[»]
7EGBelectron microscopy3.30S1-517[»]
7EGCelectron microscopy3.90S1-517[»]
7ENAelectron microscopy4.07FA1-517[»]
7ENCelectron microscopy4.13FA1-517[»]
7LBMelectron microscopy4.80S1-517[»]
7NVRelectron microscopy4.50Q1-517[»]
7NVSelectron microscopy2.80Q1-517[»]
7NVTelectron microscopy2.90Q1-517[»]
7NVUelectron microscopy2.50Q1-517[»]
7NVYelectron microscopy7.30Q1-517[»]
7NVZelectron microscopy7.20Q1-517[»]
7NW0electron microscopy6.60Q1-517[»]
BMRBiP35269
SMRiP35269
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi109217, 156 interactors
ComplexPortaliCPX-79, Transcription factor TFIIF complex
CORUMiP35269
DIPiDIP-677N
IntActiP35269, 59 interactors
MINTiP35269
STRINGi9606.ENSP00000377969

PTM databases

GlyGeniP35269, 1 site, 1 O-linked glycan (1 site)
iPTMnetiP35269
MetOSiteiP35269
PhosphoSitePlusiP35269

Genetic variation databases

BioMutaiGTF2F1
DMDMi46397744

Proteomic databases

EPDiP35269
jPOSTiP35269
MassIVEiP35269
MaxQBiP35269
PaxDbiP35269
PeptideAtlasiP35269
PRIDEiP35269
ProteomicsDBi55019
TopDownProteomicsiP35269

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...Antibodypediai		11855, 294 antibodies from 27 providers

The DNASU plasmid repository

More...DNASUi		2962

Genome annotation databases

EnsembliENST00000394456; ENSP00000377969; ENSG00000125651
GeneIDi2962
KEGGihsa:2962
MANE-SelectiENST00000394456.10; ENSP00000377969.3; NM_002096.3; NP_002087.2
UCSCiuc002meq.3, human

Organism-specific databases

Comparative Toxicogenomics Database

More...CTDi		2962
DisGeNETi2962

GeneCards: human genes, protein and diseases

More...GeneCardsi		GTF2F1
HGNCiHGNC:4652, GTF2F1
HPAiENSG00000125651, Low tissue specificity
MIMi189968, gene
neXtProtiNX_P35269
OpenTargetsiENSG00000125651
PharmGKBiPA29038
VEuPathDBiHostDB:ENSG00000125651

GenAtlas: human gene database

More...GenAtlasi		Search...

Phylogenomic databases

eggNOGiKOG2393, Eukaryota
GeneTreeiENSGT00440000038032
HOGENOMiCLU_027572_2_0_1
InParanoidiP35269
OMAiAFYVFTH
OrthoDBi893557at2759
PhylomeDBiP35269
TreeFamiTF313850

Enzyme and pathway databases

PathwayCommonsiP35269
ReactomeiR-HSA-112382, Formation of RNA Pol II elongation complex
R-HSA-113418, Formation of the Early Elongation Complex
R-HSA-167152, Formation of HIV elongation complex in the absence of HIV Tat
R-HSA-167158, Formation of the HIV-1 Early Elongation Complex
R-HSA-167160, RNA Pol II CTD phosphorylation and interaction with CE during HIV infection
R-HSA-167161, HIV Transcription Initiation
R-HSA-167162, RNA Polymerase II HIV Promoter Escape
R-HSA-167172, Transcription of the HIV genome
R-HSA-167200, Formation of HIV-1 elongation complex containing HIV-1 Tat
R-HSA-167238, Pausing and recovery of Tat-mediated HIV elongation
R-HSA-167242, Abortive elongation of HIV-1 transcript in the absence of Tat
R-HSA-167243, Tat-mediated HIV elongation arrest and recovery
R-HSA-167246, Tat-mediated elongation of the HIV-1 transcript
R-HSA-167287, HIV elongation arrest and recovery
R-HSA-167290, Pausing and recovery of HIV elongation
R-HSA-168325, Viral Messenger RNA Synthesis
R-HSA-674695, RNA Polymerase II Pre-transcription Events
R-HSA-6796648, TP53 Regulates Transcription of DNA Repair Genes
R-HSA-6803529, FGFR2 alternative splicing
R-HSA-6807505, RNA polymerase II transcribes snRNA genes
R-HSA-72086, mRNA Capping
R-HSA-72163, mRNA Splicing - Major Pathway
R-HSA-72165, mRNA Splicing - Minor Pathway
R-HSA-72203, Processing of Capped Intron-Containing Pre-mRNA
R-HSA-73776, RNA Polymerase II Promoter Escape
R-HSA-73779, RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-HSA-75953, RNA Polymerase II Transcription Initiation
R-HSA-75955, RNA Polymerase II Transcription Elongation
R-HSA-76042, RNA Polymerase II Transcription Initiation And Promoter Clearance
R-HSA-77075, RNA Pol II CTD phosphorylation and interaction with CE
R-HSA-8851708, Signaling by FGFR2 IIIa TM
R-HSA-9018519, Estrogen-dependent gene expression
SignaLinkiP35269
SIGNORiP35269

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...BioGRID-ORCSi		2962, 356 hits in 1055 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...ChiTaRSi		GTF2F1, human
EvolutionaryTraceiP35269

The Gene Wiki collection of pages on human genes and proteins

More...GeneWikii		GTF2F1

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...GenomeRNAii		2962
PharosiP35269, Tbio

Protein Ontology

More...PROi		PR:P35269
RNActiP35269, protein

The Stanford Online Universal Resource for Clones and ESTs

More...SOURCEi		Search...

Gene expression databases

BgeeiENSG00000125651, Expressed in testis and 124 other tissues
ExpressionAtlasiP35269, baseline and differential
GenevisibleiP35269, HS

Family and domain databases

Gene3Di1.10.10.10, 1 hit
IDEALiIID00369
InterProiView protein in InterPro
IPR008851, TFIIF-alpha
IPR011039, TFIIF_interaction
IPR036388, WH-like_DNA-bd_sf
IPR036390, WH_DNA-bd_sf
PANTHERiPTHR13011, PTHR13011, 1 hit
PfamiView protein in Pfam
PF05793, TFIIF_alpha, 1 hit
SUPFAMiSSF46785, SSF46785, 1 hit
SSF50916, SSF50916, 2 hits

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiT2FA_HUMAN
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P35269
Secondary accession number(s): B2RCS0, Q9BWN0
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: April 13, 2004
Last modified: February 23, 2022
This is version 211 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with genetic variants
    List of human entries with genetic variants
  3. Human variants curated from literature reports
    Index of human variants curated from literature reports
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families
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