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Protein

General transcription factor IIF subunit 1

Gene

GTF2F1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.1 Publication

Caution

Was reported to have a protein kinase activity and to autophosphorylates on Ser-385 and Thr-389.1 Publication

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • phosphatase activator activity Source: UniProtKB
  • promoter-specific chromatin binding Source: UniProtKB
  • protein domain specific binding Source: CAFA
  • protein phosphatase binding Source: CAFA
  • RNA binding Source: UniProtKB
  • transcription coactivator activity Source: ProtInc
  • transcription factor binding Source: BHF-UCL

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-112382 Formation of RNA Pol II elongation complex
R-HSA-113418 Formation of the Early Elongation Complex
R-HSA-167152 Formation of HIV elongation complex in the absence of HIV Tat
R-HSA-167158 Formation of the HIV-1 Early Elongation Complex
R-HSA-167160 RNA Pol II CTD phosphorylation and interaction with CE during HIV infection
R-HSA-167161 HIV Transcription Initiation
R-HSA-167162 RNA Polymerase II HIV Promoter Escape
R-HSA-167172 Transcription of the HIV genome
R-HSA-167200 Formation of HIV-1 elongation complex containing HIV-1 Tat
R-HSA-167238 Pausing and recovery of Tat-mediated HIV elongation
R-HSA-167242 Abortive elongation of HIV-1 transcript in the absence of Tat
R-HSA-167243 Tat-mediated HIV elongation arrest and recovery
R-HSA-167246 Tat-mediated elongation of the HIV-1 transcript
R-HSA-167287 HIV elongation arrest and recovery
R-HSA-167290 Pausing and recovery of HIV elongation
R-HSA-168325 Viral Messenger RNA Synthesis
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-HSA-6803529 FGFR2 alternative splicing
R-HSA-6807505 RNA polymerase II transcribes snRNA genes
R-HSA-72086 mRNA Capping
R-HSA-72163 mRNA Splicing - Major Pathway
R-HSA-72165 mRNA Splicing - Minor Pathway
R-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA
R-HSA-73776 RNA Polymerase II Promoter Escape
R-HSA-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-HSA-75953 RNA Polymerase II Transcription Initiation
R-HSA-75955 RNA Polymerase II Transcription Elongation
R-HSA-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-HSA-77075 RNA Pol II CTD phosphorylation and interaction with CE
R-HSA-8851708 Signaling by FGFR2 IIIa TM
R-HSA-9018519 Estrogen-dependent gene expression
SIGNORiP35269

Names & Taxonomyi

Protein namesi
Recommended name:
General transcription factor IIF subunit 1
Alternative name(s):
General transcription factor IIF 74 kDa subunit
Transcription initiation factor IIF subunit alpha
Short name:
TFIIF-alpha
Transcription initiation factor RAP74
Gene namesi
Name:GTF2F1
Synonyms:RAP74
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000125651.13
HGNCiHGNC:4652 GTF2F1
MIMi189968 gene
neXtProtiNX_P35269

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi385S → A: Eliminates putative kinase activity; when associated with A-389. 1 Publication1
Mutagenesisi389T → A: Eliminates putative kinase activity; when associated with A-385. 1 Publication1

Organism-specific databases

DisGeNETi2962
OpenTargetsiENSG00000125651
PharmGKBiPA29038

Polymorphism and mutation databases

BioMutaiGTF2F1
DMDMi46397744

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002112312 – 517General transcription factor IIF subunit 1Add BLAST516

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei156PhosphothreonineCombined sources1
Modified residuei217PhosphoserineCombined sources1
Modified residuei218PhosphoserineCombined sources1
Modified residuei221PhosphoserineCombined sources1
Modified residuei224PhosphoserineCombined sources1
Modified residuei331PhosphothreonineCombined sources1
Modified residuei377PhosphoserineCombined sources1
Modified residuei380PhosphoserineCombined sources1
Modified residuei381PhosphoserineCombined sources1
Modified residuei385PhosphoserineCombined sources1 Publication1
Modified residuei389PhosphothreonineCombined sources1 Publication1
Modified residuei391PhosphoserineCombined sources1
Modified residuei407N6-acetyllysineCombined sources1
Modified residuei431PhosphoserineCombined sources1
Modified residuei433PhosphoserineCombined sources1
Modified residuei436PhosphoserineCombined sources1
Modified residuei437PhosphothreonineCombined sources1
Modified residuei446PhosphothreonineCombined sources1
Modified residuei449PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated on Ser and other residues by TAF1 and casein kinase II-like kinases.2 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP35269
MaxQBiP35269
PaxDbiP35269
PeptideAtlasiP35269
PRIDEiP35269
ProteomicsDBi55019
TopDownProteomicsiP35269

PTM databases

iPTMnetiP35269
PhosphoSitePlusiP35269

Miscellaneous databases

PMAP-CutDBiP35269

Expressioni

Inductioni

Up-regulated in response to enterovirus 71 (EV71) infection.1 Publication

Gene expression databases

BgeeiENSG00000125651 Expressed in 98 organ(s), highest expression level in testis
CleanExiHS_GTF2F1
ExpressionAtlasiP35269 baseline and differential
GenevisibleiP35269 HS

Organism-specific databases

HPAiHPA022793
HPA028707
HPA070752

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit. Interacts with GTF2F2, CTDP1, TAF6/TAFII80 and URI1. Interacts with GTF2B (via C-terminus and preferentially via acetylated form); this interaction prevents binding of GTF2B to GTF2F2 (PubMed:8662660, PubMed:12931194).6 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi109217, 109 interactors
ComplexPortaliCPX-79 Transcription factor TFIIF complex
CORUMiP35269
DIPiDIP-677N
IntActiP35269, 29 interactors
MINTiP35269
STRINGi9606.ENSP00000377969

Structurei

Secondary structure

1517
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP35269
SMRiP35269
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP35269

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi283 – 350Glu-richAdd BLAST68

Sequence similaritiesi

Belongs to the TFIIF alpha subunit family.Curated

Phylogenomic databases

eggNOGiKOG2393 Eukaryota
ENOG410XSRW LUCA
GeneTreeiENSGT00440000038032
HOGENOMiHOG000059644
HOVERGENiHBG000729
InParanoidiP35269
KOiK03138
OMAiGAIEAYP
OrthoDBiEOG091G0N3J
PhylomeDBiP35269
TreeFamiTF313850

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR008851 TFIIF-alpha
IPR011039 TFIIF_interaction
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR13011 PTHR13011, 1 hit
PfamiView protein in Pfam
PF05793 TFIIF_alpha, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF50916 SSF50916, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

P35269-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAALGPSSQN VTEYVVRVPK NTTKKYNIMA FNAADKVNFA TWNQARLERD
60 70 80 90 100
LSNKKIYQEE EMPESGAGSE FNRKLREEAR RKKYGIVLKE FRPEDQPWLL
110 120 130 140 150
RVNGKSGRKF KGIKKGGVTE NTSYYIFTQC PDGAFEAFPV HNWYNFTPLA
160 170 180 190 200
RHRTLTAEEA EEEWERRNKV LNHFSIMQQR RLKDQDQDED EEEKEKRGRR
210 220 230 240 250
KASELRIHDL EDDLEMSSDA SDASGEEGGR VPKAKKKAPL AKGGRKKKKK
260 270 280 290 300
KGSDDEAFED SDDGDFEGQE VDYMSDGSSS SQEEPESKAK APQQEEGPKG
310 320 330 340 350
VDEQSDSSEE SEEEKPPEED KEEEEEKKAP TPQEKKRRKD SSEESDSSEE
360 370 380 390 400
SDIDSEASSA LFMAKKKTPP KRERKPSGGS SRGNSRPGTP SAEGGSTSST
410 420 430 440 450
LRAAASKLEQ GKRVSEMPAA KRLRLDTGPQ SLSGKSTPQP PSGKTTPNSG
460 470 480 490 500
DVQVTEDAVR RYLTRKPMTT KDLLKKFQTK KTGLSSEQTV NVLAQILKRL
510
NPERKMINDK MHFSLKE
Length:517
Mass (Da):58,240
Last modified:April 13, 2004 - v2
Checksum:i1032B04A36BDC24F
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
M0QXD6M0QXD6_HUMAN
General transcription factor IIF su...
GTF2F1
433Annotation score:
M0R0R9M0R0R9_HUMAN
General transcription factor IIF su...
GTF2F1
381Annotation score:
M0R0Z3M0R0Z3_HUMAN
General transcription factor IIF su...
GTF2F1
127Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti231V → I in CAA45404 (PubMed:1734284).Curated1
Sequence conflicti361L → F in CAA45408 (PubMed:1734283).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0390043A → V. Corresponds to variant dbSNP:rs34826931Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64037 mRNA Translation: CAA45408.1
X64002 mRNA Translation: CAA45404.1
BT007097 mRNA Translation: AAP35761.1
AK315240 mRNA Translation: BAG37667.1
CH471139 Genomic DNA Translation: EAW69098.1
BC000120 mRNA Translation: AAH00120.1
BC013007 mRNA Translation: AAH13007.1
CCDSiCCDS12165.1
PIRiS20248
RefSeqiNP_002087.2, NM_002096.2
UniGeneiHs.68257

Genome annotation databases

EnsembliENST00000394456; ENSP00000377969; ENSG00000125651
GeneIDi2962
KEGGihsa:2962
UCSCiuc002meq.3 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64037 mRNA Translation: CAA45408.1
X64002 mRNA Translation: CAA45404.1
BT007097 mRNA Translation: AAP35761.1
AK315240 mRNA Translation: BAG37667.1
CH471139 Genomic DNA Translation: EAW69098.1
BC000120 mRNA Translation: AAH00120.1
BC013007 mRNA Translation: AAH13007.1
CCDSiCCDS12165.1
PIRiS20248
RefSeqiNP_002087.2, NM_002096.2
UniGeneiHs.68257

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F3UX-ray1.70B/D/F/H2-172[»]
1I27X-ray1.02A449-517[»]
1J2XX-ray2.00A449-517[»]
1NHANMR-A436-517[»]
1ONVNMR-A436-517[»]
2K7LNMR-A451-517[»]
5IY6electron microscopy7.20S1-517[»]
5IY7electron microscopy8.60S1-517[»]
5IY8electron microscopy7.90S1-517[»]
5IY9electron microscopy6.30S1-517[»]
5IYAelectron microscopy5.40S1-517[»]
5IYBelectron microscopy3.90S1-517[»]
5IYCelectron microscopy3.90S1-517[»]
5IYDelectron microscopy3.90S1-517[»]
ProteinModelPortaliP35269
SMRiP35269
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109217, 109 interactors
ComplexPortaliCPX-79 Transcription factor TFIIF complex
CORUMiP35269
DIPiDIP-677N
IntActiP35269, 29 interactors
MINTiP35269
STRINGi9606.ENSP00000377969

PTM databases

iPTMnetiP35269
PhosphoSitePlusiP35269

Polymorphism and mutation databases

BioMutaiGTF2F1
DMDMi46397744

Proteomic databases

EPDiP35269
MaxQBiP35269
PaxDbiP35269
PeptideAtlasiP35269
PRIDEiP35269
ProteomicsDBi55019
TopDownProteomicsiP35269

Protocols and materials databases

DNASUi2962
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000394456; ENSP00000377969; ENSG00000125651
GeneIDi2962
KEGGihsa:2962
UCSCiuc002meq.3 human

Organism-specific databases

CTDi2962
DisGeNETi2962
EuPathDBiHostDB:ENSG00000125651.13
GeneCardsiGTF2F1
HGNCiHGNC:4652 GTF2F1
HPAiHPA022793
HPA028707
HPA070752
MIMi189968 gene
neXtProtiNX_P35269
OpenTargetsiENSG00000125651
PharmGKBiPA29038
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2393 Eukaryota
ENOG410XSRW LUCA
GeneTreeiENSGT00440000038032
HOGENOMiHOG000059644
HOVERGENiHBG000729
InParanoidiP35269
KOiK03138
OMAiGAIEAYP
OrthoDBiEOG091G0N3J
PhylomeDBiP35269
TreeFamiTF313850

Enzyme and pathway databases

ReactomeiR-HSA-112382 Formation of RNA Pol II elongation complex
R-HSA-113418 Formation of the Early Elongation Complex
R-HSA-167152 Formation of HIV elongation complex in the absence of HIV Tat
R-HSA-167158 Formation of the HIV-1 Early Elongation Complex
R-HSA-167160 RNA Pol II CTD phosphorylation and interaction with CE during HIV infection
R-HSA-167161 HIV Transcription Initiation
R-HSA-167162 RNA Polymerase II HIV Promoter Escape
R-HSA-167172 Transcription of the HIV genome
R-HSA-167200 Formation of HIV-1 elongation complex containing HIV-1 Tat
R-HSA-167238 Pausing and recovery of Tat-mediated HIV elongation
R-HSA-167242 Abortive elongation of HIV-1 transcript in the absence of Tat
R-HSA-167243 Tat-mediated HIV elongation arrest and recovery
R-HSA-167246 Tat-mediated elongation of the HIV-1 transcript
R-HSA-167287 HIV elongation arrest and recovery
R-HSA-167290 Pausing and recovery of HIV elongation
R-HSA-168325 Viral Messenger RNA Synthesis
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-HSA-6803529 FGFR2 alternative splicing
R-HSA-6807505 RNA polymerase II transcribes snRNA genes
R-HSA-72086 mRNA Capping
R-HSA-72163 mRNA Splicing - Major Pathway
R-HSA-72165 mRNA Splicing - Minor Pathway
R-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA
R-HSA-73776 RNA Polymerase II Promoter Escape
R-HSA-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-HSA-75953 RNA Polymerase II Transcription Initiation
R-HSA-75955 RNA Polymerase II Transcription Elongation
R-HSA-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-HSA-77075 RNA Pol II CTD phosphorylation and interaction with CE
R-HSA-8851708 Signaling by FGFR2 IIIa TM
R-HSA-9018519 Estrogen-dependent gene expression
SIGNORiP35269

Miscellaneous databases

ChiTaRSiGTF2F1 human
EvolutionaryTraceiP35269
GeneWikiiGTF2F1
GenomeRNAii2962
PMAP-CutDBiP35269
PROiPR:P35269
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000125651 Expressed in 98 organ(s), highest expression level in testis
CleanExiHS_GTF2F1
ExpressionAtlasiP35269 baseline and differential
GenevisibleiP35269 HS

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR008851 TFIIF-alpha
IPR011039 TFIIF_interaction
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR13011 PTHR13011, 1 hit
PfamiView protein in Pfam
PF05793 TFIIF_alpha, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF50916 SSF50916, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiT2FA_HUMAN
AccessioniPrimary (citable) accession number: P35269
Secondary accession number(s): B2RCS0, Q9BWN0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: April 13, 2004
Last modified: October 10, 2018
This is version 191 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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