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Protein

Replication factor C subunit 1

Gene

RFC1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA.1 Publication
Interacts with C-terminus of PCNA. 5' phosphate residue is required for binding of the N-terminal DNA-binding domain to duplex DNA, suggesting a role in recognition of non-primer template DNA structures during replication and/or repair.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi650 – 657ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: ProtInc
  • DNA binding Source: GO_Central
  • DNA clamp loader activity Source: InterPro
  • DNA clamp unloader activity Source: UniProtKB
  • double-stranded DNA binding Source: Ensembl
  • enzyme activator activity Source: ProtInc
  • protein domain specific binding Source: Ensembl
  • sequence-specific DNA binding Source: Ensembl

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processDNA replication, Transcription, Transcription regulation
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-110312 Translesion synthesis by REV1
R-HSA-110314 Recognition of DNA damage by PCNA-containing replication complex
R-HSA-110320 Translesion Synthesis by POLH
R-HSA-174411 Polymerase switching on the C-strand of the telomere
R-HSA-5651801 PCNA-Dependent Long Patch Base Excision Repair
R-HSA-5655862 Translesion synthesis by POLK
R-HSA-5656121 Translesion synthesis by POLI
R-HSA-5656169 Termination of translesion DNA synthesis
R-HSA-5685942 HDR through Homologous Recombination (HRR)
R-HSA-5696397 Gap-filling DNA repair synthesis and ligation in GG-NER
R-HSA-5696400 Dual Incision in GG-NER
R-HSA-6782135 Dual incision in TC-NER
R-HSA-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-HSA-69091 Polymerase switching
SignaLinkiP35251

Names & Taxonomyi

Protein namesi
Recommended name:
Replication factor C subunit 1
Alternative name(s):
Activator 1 140 kDa subunit
Short name:
A1 140 kDa subunit
Activator 1 large subunit
Activator 1 subunit 1
DNA-binding protein PO-GA
Replication factor C 140 kDa subunit
Short name:
RF-C 140 kDa subunit
Short name:
RFC140
Replication factor C large subunit
Gene namesi
Name:RFC1
Synonyms:RFC140
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

EuPathDBiHostDB:ENSG00000035928.14
HGNCiHGNC:9969 RFC1
MIMi102579 gene
neXtProtiNX_P35251

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi5981
OpenTargetsiENSG00000035928
PharmGKBiPA34338

Polymorphism and mutation databases

BioMutaiRFC1
DMDMi56757608

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001217721 – 1148Replication factor C subunit 1Add BLAST1148

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki50Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei67PhosphotyrosineCombined sources1
Modified residuei69PhosphoserineCombined sources1
Modified residuei71PhosphoserineCombined sources1
Modified residuei73PhosphoserineCombined sources1
Modified residuei108PhosphoserineCombined sources1
Modified residuei110PhosphothreonineCombined sources1
Modified residuei156PhosphoserineCombined sources1
Modified residuei161PhosphothreonineCombined sources1
Modified residuei163PhosphothreonineCombined sources1
Modified residuei164PhosphoserineCombined sources1
Modified residuei173PhosphoserineCombined sources1
Modified residuei190PhosphoserineCombined sources1
Modified residuei253PhosphoserineBy similarity1
Modified residuei281PhosphoserineCombined sources1
Modified residuei283PhosphoserineCombined sources1
Modified residuei312PhosphoserineCombined sources1
Modified residuei368PhosphoserineCombined sources1
Modified residuei537PhosphoserineBy similarity1
Modified residuei1104PhosphoserineCombined sources1
Modified residuei1106PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP35251
MaxQBiP35251
PaxDbiP35251
PeptideAtlasiP35251
PRIDEiP35251
ProteomicsDBi55016
55017 [P35251-2]

PTM databases

iPTMnetiP35251
PhosphoSitePlusiP35251

Miscellaneous databases

PMAP-CutDBiP35251

Expressioni

Tissue specificityi

Wide tissue distribution. Undetectable in placental tissue.

Gene expression databases

BgeeiENSG00000035928 Expressed in 216 organ(s), highest expression level in corpus callosum
CleanExiHS_RFC1
ExpressionAtlasiP35251 baseline and differential
GenevisibleiP35251 HS

Organism-specific databases

HPAiCAB009520
HPA046116
HPA069306

Interactioni

Subunit structurei

Heterotetramer of subunits RFC2, RFC3, RFC4 and RFC5 that can form a complex either with RFC1 or with RAD17. The former interacts with PCNA in the presence of ATP, while the latter has ATPase activity but is not stimulated by PCNA.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
RFC2P352504EBI-476616,EBI-476409

GO - Molecular functioni

Protein-protein interaction databases

BioGridi111913, 78 interactors
ComplexPortaliCPX-415 DNA replication factor C complex
CORUMiP35251
IntActiP35251, 34 interactors
MINTiP35251
STRINGi9606.ENSP00000371321

Structurei

Secondary structure

11148
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP35251
SMRiP35251
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP35251

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini402 – 492BRCTPROSITE-ProRule annotationAdd BLAST91

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1120 – 1124Nuclear localization signalSequence analysis5

Sequence similaritiesi

Belongs to the activator 1 large subunit family.Curated

Phylogenomic databases

eggNOGiENOG410IPR8 Eukaryota
COG5275 LUCA
GeneTreeiENSGT00730000111066
HOGENOMiHOG000013116
HOVERGENiHBG004167
InParanoidiP35251
KOiK10754
OMAiRWLQNWH
OrthoDBiEOG091G04EX
PhylomeDBiP35251
TreeFamiTF105722

Family and domain databases

CDDicd00027 BRCT, 1 hit
Gene3Di3.40.50.10190, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR003959 ATPase_AAA_core
IPR001357 BRCT_dom
IPR036420 BRCT_dom_sf
IPR008921 DNA_pol3_clamp-load_cplx_C
IPR013725 DNA_replication_fac_RFC1_C
IPR027417 P-loop_NTPase
IPR012178 RFC1
PfamiView protein in Pfam
PF00004 AAA, 1 hit
PF00533 BRCT, 1 hit
PF08519 RFC1, 1 hit
PIRSFiPIRSF036578 RFC1, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SM00292 BRCT, 1 hit
SUPFAMiSSF48019 SSF48019, 1 hit
SSF52113 SSF52113, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS50172 BRCT, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P35251-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDIRKFFGVI PSGKKLVSET VKKNEKTKSD EETLKAKKGI KEIKVNSSRK
60 70 80 90 100
EDDFKQKQPS KKKRIIYDSD SESEETLQVK NAKKPPEKLP VSSKPGKISR
110 120 130 140 150
QDPVTYISET DEEDDFMCKK AASKSKENGR STNSHLGTSN MKKNEENTKT
160 170 180 190 200
KNKPLSPIKL TPTSVLDYFG TGSVQRSNKK MVASKRKELS QNTDESGLND
210 220 230 240 250
EAIAKQLQLD EDAELERQLH EDEEFARTLA MLDEEPKTKK ARKDTEAGET
260 270 280 290 300
FSSVQANLSK AEKHKYPHKV KTAQVSDERK SYSPRKQSKY ESSKESQQHS
310 320 330 340 350
KSSADKIGEV SSPKASSKLA IMKRKEESSY KEIEPVASKR KENAIKLKGE
360 370 380 390 400
TKTPKKTKSS PAKKESVSPE DSEKKRTNYQ AYRSYLNREG PKALGSKEIP
410 420 430 440 450
KGAENCLEGL IFVITGVLES IERDEAKSLI ERYGGKVTGN VSKKTNYLVM
460 470 480 490 500
GRDSGQSKSD KAAALGTKII DEDGLLNLIR TMPGKKSKYE IAVETEMKKE
510 520 530 540 550
SKLERTPQKN VQGKRKISPS KKESESKKSR PTSKRDSLAK TIKKETDVFW
560 570 580 590 600
KSLDFKEQVA EETSGDSKAR NLADDSSENK VENLLWVDKY KPTSLKTIIG
610 620 630 640 650
QQGDQSCANK LLRWLRNWQK SSSEDKKHAA KFGKFSGKDD GSSFKAALLS
660 670 680 690 700
GPPGVGKTTT ASLVCQELGY SYVELNASDT RSKSSLKAIV AESLNNTSIK
710 720 730 740 750
GFYSNGAASS VSTKHALIMD EVDGMAGNED RGGIQELIGL IKHTKIPIIC
760 770 780 790 800
MCNDRNHPKI RSLVHYCFDL RFQRPRVEQI KGAMMSIAFK EGLKIPPPAM
810 820 830 840 850
NEIILGANQD IRQVLHNLSM WCARSKALTY DQAKADSHRA KKDIKMGPFD
860 870 880 890 900
VARKVFAAGE ETAHMSLVDK SDLFFHDYSI APLFVQENYI HVKPVAAGGD
910 920 930 940 950
MKKHLMLLSR AADSICDGDL VDSQIRSKQN WSLLPAQAIY ASVLPGELMR
960 970 980 990 1000
GYMTQFPTFP SWLGKHSSTG KHDRIVQDLA LHMSLRTYSS KRTVNMDYLS
1010 1020 1030 1040 1050
LLRDALVQPL TSQGVDGVQD VVALMDTYYL MKEDFENIME ISSWGGKPSP
1060 1070 1080 1090 1100
FSKLDPKVKA AFTRAYNKEA HLTPYSLQAI KASRHSTSPS LDSEYNEELN
1110 1120 1130 1140
EDDSQSDEKD QDAIETDAMI KKKTKSSKPS KPEKDKEPRK GKGKSSKK
Length:1,148
Mass (Da):128,255
Last modified:December 21, 2004 - v4
Checksum:i485F0332FB56819B
GO
Isoform 2 (identifier: P35251-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     630-630: Missing.

Note: Alternative use of an acceptor site.
Show »
Length:1,147
Mass (Da):128,183
Checksum:iD40A9F6F5CEB61AF
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H0Y9P8H0Y9P8_HUMAN
Replication factor C subunit 1
RFC1
101Annotation score:
H0Y8U4H0Y8U4_HUMAN
Replication factor C subunit 1
RFC1
187Annotation score:
E0CX09E0CX09_HUMAN
Replication factor C subunit 1
RFC1
130Annotation score:
D6RAD2D6RAD2_HUMAN
Replication factor C subunit 1
RFC1
132Annotation score:
A0A1W2PP02A0A1W2PP02_HUMAN
Replication factor C subunit 1
RFC1
289Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti326E → K in AAA16121 (PubMed:8248204).Curated1
Sequence conflicti567S → N in AAH51786 (PubMed:15489334).Curated1
Sequence conflicti629A → S (PubMed:8512577).Curated1
Sequence conflicti629A → S (PubMed:7914507).Curated1
Sequence conflicti629A → S (Ref. 4) Curated1
Sequence conflicti641G → N (PubMed:8512577).Curated1
Sequence conflicti641G → N (PubMed:7914507).Curated1
Sequence conflicti641G → N (Ref. 4) Curated1
Sequence conflicti677A → R (PubMed:8512577).Curated1
Sequence conflicti677A → R (PubMed:7914507).Curated1
Sequence conflicti677A → R (Ref. 4) Curated1
Sequence conflicti1076S → A (PubMed:8512577).Curated1
Sequence conflicti1076S → A (PubMed:7914507).Curated1
Sequence conflicti1076S → A (Ref. 4) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_014860598I → V1 PublicationCorresponds to variant dbSNP:rs2066791Ensembl.1
Natural variantiVAR_016986613R → L3 PublicationsCorresponds to variant dbSNP:rs1057747Ensembl.1
Natural variantiVAR_020657692E → D1 PublicationCorresponds to variant dbSNP:rs11932767Ensembl.1
Natural variantiVAR_020658955Q → K1 PublicationCorresponds to variant dbSNP:rs17335452Ensembl.1
Natural variantiVAR_0206591146S → L1 PublicationCorresponds to variant dbSNP:rs17288828Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_008443630Missing in isoform 2. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L23320 mRNA Translation: AAA16121.1
Z22642 mRNA Translation: CAA80355.1
AF040250 mRNA Translation: AAB99788.1
AY600371 Genomic DNA Translation: AAS94325.1
AK291612 mRNA Translation: BAF84301.1
CH471069 Genomic DNA Translation: EAW92923.1
BC035297 mRNA Translation: AAH35297.1
BC051751 mRNA Translation: AAH51751.1
BC051786 mRNA Translation: AAH51786.1
CCDSiCCDS3450.1 [P35251-2]
CCDS56329.1 [P35251-1]
PIRiA49651
JN0599
RefSeqiNP_001191676.1, NM_001204747.1 [P35251-1]
NP_002904.3, NM_002913.4 [P35251-2]
UniGeneiHs.507475

Genome annotation databases

EnsembliENST00000349703; ENSP00000261424; ENSG00000035928 [P35251-2]
ENST00000381897; ENSP00000371321; ENSG00000035928 [P35251-1]
GeneIDi5981
KEGGihsa:5981
UCSCiuc003gtx.3 human [P35251-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L23320 mRNA Translation: AAA16121.1
Z22642 mRNA Translation: CAA80355.1
AF040250 mRNA Translation: AAB99788.1
AY600371 Genomic DNA Translation: AAS94325.1
AK291612 mRNA Translation: BAF84301.1
CH471069 Genomic DNA Translation: EAW92923.1
BC035297 mRNA Translation: AAH35297.1
BC051751 mRNA Translation: AAH51751.1
BC051786 mRNA Translation: AAH51786.1
CCDSiCCDS3450.1 [P35251-2]
CCDS56329.1 [P35251-1]
PIRiA49651
JN0599
RefSeqiNP_001191676.1, NM_001204747.1 [P35251-1]
NP_002904.3, NM_002913.4 [P35251-2]
UniGeneiHs.507475

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EBUNMR-A392-496[»]
2K6GNMR-A375-480[»]
2K7FNMR-A375-480[»]
ProteinModelPortaliP35251
SMRiP35251
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111913, 78 interactors
ComplexPortaliCPX-415 DNA replication factor C complex
CORUMiP35251
IntActiP35251, 34 interactors
MINTiP35251
STRINGi9606.ENSP00000371321

PTM databases

iPTMnetiP35251
PhosphoSitePlusiP35251

Polymorphism and mutation databases

BioMutaiRFC1
DMDMi56757608

Proteomic databases

EPDiP35251
MaxQBiP35251
PaxDbiP35251
PeptideAtlasiP35251
PRIDEiP35251
ProteomicsDBi55016
55017 [P35251-2]

Protocols and materials databases

DNASUi5981
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000349703; ENSP00000261424; ENSG00000035928 [P35251-2]
ENST00000381897; ENSP00000371321; ENSG00000035928 [P35251-1]
GeneIDi5981
KEGGihsa:5981
UCSCiuc003gtx.3 human [P35251-1]

Organism-specific databases

CTDi5981
DisGeNETi5981
EuPathDBiHostDB:ENSG00000035928.14
GeneCardsiRFC1
HGNCiHGNC:9969 RFC1
HPAiCAB009520
HPA046116
HPA069306
MIMi102579 gene
neXtProtiNX_P35251
OpenTargetsiENSG00000035928
PharmGKBiPA34338
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IPR8 Eukaryota
COG5275 LUCA
GeneTreeiENSGT00730000111066
HOGENOMiHOG000013116
HOVERGENiHBG004167
InParanoidiP35251
KOiK10754
OMAiRWLQNWH
OrthoDBiEOG091G04EX
PhylomeDBiP35251
TreeFamiTF105722

Enzyme and pathway databases

ReactomeiR-HSA-110312 Translesion synthesis by REV1
R-HSA-110314 Recognition of DNA damage by PCNA-containing replication complex
R-HSA-110320 Translesion Synthesis by POLH
R-HSA-174411 Polymerase switching on the C-strand of the telomere
R-HSA-5651801 PCNA-Dependent Long Patch Base Excision Repair
R-HSA-5655862 Translesion synthesis by POLK
R-HSA-5656121 Translesion synthesis by POLI
R-HSA-5656169 Termination of translesion DNA synthesis
R-HSA-5685942 HDR through Homologous Recombination (HRR)
R-HSA-5696397 Gap-filling DNA repair synthesis and ligation in GG-NER
R-HSA-5696400 Dual Incision in GG-NER
R-HSA-6782135 Dual incision in TC-NER
R-HSA-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-HSA-69091 Polymerase switching
SignaLinkiP35251

Miscellaneous databases

ChiTaRSiRFC1 human
EvolutionaryTraceiP35251
GeneWikiiRFC1
GenomeRNAii5981
PMAP-CutDBiP35251
PROiPR:P35251
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000035928 Expressed in 216 organ(s), highest expression level in corpus callosum
CleanExiHS_RFC1
ExpressionAtlasiP35251 baseline and differential
GenevisibleiP35251 HS

Family and domain databases

CDDicd00027 BRCT, 1 hit
Gene3Di3.40.50.10190, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR003959 ATPase_AAA_core
IPR001357 BRCT_dom
IPR036420 BRCT_dom_sf
IPR008921 DNA_pol3_clamp-load_cplx_C
IPR013725 DNA_replication_fac_RFC1_C
IPR027417 P-loop_NTPase
IPR012178 RFC1
PfamiView protein in Pfam
PF00004 AAA, 1 hit
PF00533 BRCT, 1 hit
PF08519 RFC1, 1 hit
PIRSFiPIRSF036578 RFC1, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SM00292 BRCT, 1 hit
SUPFAMiSSF48019 SSF48019, 1 hit
SSF52113 SSF52113, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS50172 BRCT, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRFC1_HUMAN
AccessioniPrimary (citable) accession number: P35251
Secondary accession number(s): A8K6E7
, Q5XKF5, Q6PKU0, Q86V41, Q86V46
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: December 21, 2004
Last modified: November 7, 2018
This is version 201 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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