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Protein

Prohibitin

Gene

PHB

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processDNA synthesis

Enzyme and pathway databases

ReactomeiR-HSA-5673000 RAF activation
R-HSA-6802946 Signaling by moderate kinase activity BRAF mutants
R-HSA-6802949 Signaling by RAS mutants
R-HSA-6802955 Paradoxical activation of RAF signaling by kinase inactive BRAF
R-HSA-8949664 Processing of SMDT1

Names & Taxonomyi

Protein namesi
Recommended name:
Prohibitin
Gene namesi
Name:PHB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000167085.11
HGNCiHGNC:8912 PHB
MIMi176705 gene
neXtProtiNX_P35232

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

Organism-specific databases

DisGeNETi5245
MalaCardsiPHB
OpenTargetsiENSG00000167085
PharmGKBiPA33251

Polymorphism and mutation databases

DMDMi464371

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002138782 – 272ProhibitinAdd BLAST271

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei91PhosphothreonineCombined sources1
Modified residuei128N6-acetyllysineBy similarity1
Modified residuei186N6-acetyllysineBy similarity1
Modified residuei202N6-acetyllysine; alternateCombined sources1
Modified residuei202N6-succinyllysine; alternateBy similarity1
Modified residuei249PhosphotyrosineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP35232
MaxQBiP35232
PaxDbiP35232
PeptideAtlasiP35232
PRIDEiP35232
ProteomicsDBi54995
TopDownProteomicsiP35232-1 [P35232-1]

2D gel databases

DOSAC-COBS-2DPAGEiP35232
OGPiP35232
REPRODUCTION-2DPAGEiIPI00017334
P35232
SWISS-2DPAGEiP35232
UCD-2DPAGEiP35232

PTM databases

iPTMnetiP35232
PhosphoSitePlusiP35232
SwissPalmiP35232

Expressioni

Tissue specificityi

Widely expressed in different tissues.

Developmental stagei

Levels of expression in fibroblasts decrease heterogeneously during cellular aging.1 Publication

Inductioni

Expression increases approximately 3-fold upon entry into G1 phase compared with other phases of the cell cycle. Also induced following inhibition of mitochondrial protein synthesis by thiamphenicol.1 Publication

Gene expression databases

BgeeiENSG00000167085 Expressed in 231 organ(s), highest expression level in mucosa of transverse colon
CleanExiHS_PHB
ExpressionAtlasiP35232 baseline and differential
GenevisibleiP35232 HS

Organism-specific databases

HPAiHPA003280

Interactioni

Subunit structurei

Interacts with PHB2. Interacts with STOML2.2 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi111264, 158 interactors
CORUMiP35232
DIPiDIP-24248N
IntActiP35232, 105 interactors
MINTiP35232
STRINGi9606.ENSP00000300408

Structurei

3D structure databases

ProteinModelPortaliP35232
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili177 – 211Sequence analysisAdd BLAST35

Sequence similaritiesi

Belongs to the prohibitin family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3083 Eukaryota
COG0330 LUCA
GeneTreeiENSGT00550000074770
HOGENOMiHOG000205692
HOVERGENiHBG004457
InParanoidiP35232
KOiK17080
OMAiRTRIRDN
OrthoDBiEOG091G0IHW
PhylomeDBiP35232
TreeFamiTF300095

Family and domain databases

CDDicd03401 SPFH_prohibitin, 1 hit
InterProiView protein in InterPro
IPR001107 Band_7
IPR036013 Band_7/SPFH_dom_sf
IPR000163 Prohibitin
PANTHERiPTHR23222 PTHR23222, 1 hit
PfamiView protein in Pfam
PF01145 Band_7, 1 hit
PRINTSiPR00679 PROHIBITIN
SMARTiView protein in SMART
SM00244 PHB, 1 hit
SUPFAMiSSF117892 SSF117892, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P35232-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAAKVFESIG KFGLALAVAG GVVNSALYNV DAGHRAVIFD RFRGVQDIVV
60 70 80 90 100
GEGTHFLIPW VQKPIIFDCR SRPRNVPVIT GSKDLQNVNI TLRILFRPVA
110 120 130 140 150
SQLPRIFTSI GEDYDERVLP SITTEILKSV VARFDAGELI TQRELVSRQV
160 170 180 190 200
SDDLTERAAT FGLILDDVSL THLTFGKEFT EAVEAKQVAQ QEAERARFVV
210 220 230 240 250
EKAEQQKKAA IISAEGDSKA AELIANSLAT AGDGLIELRK LEAAEDIAYQ
260 270
LSRSRNITYL PAGQSVLLQL PQ
Length:272
Mass (Da):29,804
Last modified:February 1, 1994 - v1
Checksum:i915494273E342C76
GO
Isoform 2 (identifier: P35232-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     140-256: Missing.

Note: No experimental confirmation available.
Show »
Length:155
Mass (Da):17,018
Checksum:iFDFDE03209523798
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9JW96C9JW96_HUMAN
Prohibitin
PHB
246Annotation score:
E9PCW0E9PCW0_HUMAN
Prohibitin
PHB
216Annotation score:
C9JZ20C9JZ20_HUMAN
Prohibitin
PHB
201Annotation score:
E7ESE2E7ESE2_HUMAN
Prohibitin
PHB
202Annotation score:
D6RBK0D6RBK0_HUMAN
Prohibitin
PHB
124Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_00647988V → A in a breast cancer sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs121918373Ensembl.1
Natural variantiVAR_006480105R → H in a breast cancer sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs121918372Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_054922140 – 256Missing in isoform 2. 1 PublicationAdd BLAST117

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S85655 mRNA Translation: AAB21614.1
L14272 Genomic DNA Translation: AAO18340.1
BT007411 mRNA Translation: AAP36079.1
AK302258 mRNA Translation: BAG63609.1
AC091180 Genomic DNA No translation available.
BC013401 mRNA Translation: AAH13401.1
BC095460 mRNA Translation: AAH95460.1
CCDSiCCDS11548.1 [P35232-1]
CCDS62244.1 [P35232-2]
PIRiI52690
RefSeqiNP_001268425.1, NM_001281496.1 [P35232-1]
NP_001268426.1, NM_001281497.1 [P35232-2]
NP_001268644.1, NM_001281715.1 [P35232-1]
NP_002625.1, NM_002634.3 [P35232-1]
XP_016880252.1, XM_017024763.1 [P35232-1]
UniGeneiHs.514303

Genome annotation databases

EnsembliENST00000300408; ENSP00000300408; ENSG00000167085 [P35232-1]
ENST00000511832; ENSP00000425035; ENSG00000167085 [P35232-2]
ENST00000614445; ENSP00000479488; ENSG00000167085 [P35232-1]
ENST00000617874; ENSP00000484113; ENSG00000167085 [P35232-1]
GeneIDi5245
KEGGihsa:5245
UCSCiuc002iox.3 human [P35232-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S85655 mRNA Translation: AAB21614.1
L14272 Genomic DNA Translation: AAO18340.1
BT007411 mRNA Translation: AAP36079.1
AK302258 mRNA Translation: BAG63609.1
AC091180 Genomic DNA No translation available.
BC013401 mRNA Translation: AAH13401.1
BC095460 mRNA Translation: AAH95460.1
CCDSiCCDS11548.1 [P35232-1]
CCDS62244.1 [P35232-2]
PIRiI52690
RefSeqiNP_001268425.1, NM_001281496.1 [P35232-1]
NP_001268426.1, NM_001281497.1 [P35232-2]
NP_001268644.1, NM_001281715.1 [P35232-1]
NP_002625.1, NM_002634.3 [P35232-1]
XP_016880252.1, XM_017024763.1 [P35232-1]
UniGeneiHs.514303

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LU7model-A1-272[»]
ProteinModelPortaliP35232
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111264, 158 interactors
CORUMiP35232
DIPiDIP-24248N
IntActiP35232, 105 interactors
MINTiP35232
STRINGi9606.ENSP00000300408

PTM databases

iPTMnetiP35232
PhosphoSitePlusiP35232
SwissPalmiP35232

Polymorphism and mutation databases

DMDMi464371

2D gel databases

DOSAC-COBS-2DPAGEiP35232
OGPiP35232
REPRODUCTION-2DPAGEiIPI00017334
P35232
SWISS-2DPAGEiP35232
UCD-2DPAGEiP35232

Proteomic databases

EPDiP35232
MaxQBiP35232
PaxDbiP35232
PeptideAtlasiP35232
PRIDEiP35232
ProteomicsDBi54995
TopDownProteomicsiP35232-1 [P35232-1]

Protocols and materials databases

DNASUi5245
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000300408; ENSP00000300408; ENSG00000167085 [P35232-1]
ENST00000511832; ENSP00000425035; ENSG00000167085 [P35232-2]
ENST00000614445; ENSP00000479488; ENSG00000167085 [P35232-1]
ENST00000617874; ENSP00000484113; ENSG00000167085 [P35232-1]
GeneIDi5245
KEGGihsa:5245
UCSCiuc002iox.3 human [P35232-1]

Organism-specific databases

CTDi5245
DisGeNETi5245
EuPathDBiHostDB:ENSG00000167085.11
GeneCardsiPHB
HGNCiHGNC:8912 PHB
HPAiHPA003280
MalaCardsiPHB
MIMi176705 gene
neXtProtiNX_P35232
OpenTargetsiENSG00000167085
PharmGKBiPA33251
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3083 Eukaryota
COG0330 LUCA
GeneTreeiENSGT00550000074770
HOGENOMiHOG000205692
HOVERGENiHBG004457
InParanoidiP35232
KOiK17080
OMAiRTRIRDN
OrthoDBiEOG091G0IHW
PhylomeDBiP35232
TreeFamiTF300095

Enzyme and pathway databases

ReactomeiR-HSA-5673000 RAF activation
R-HSA-6802946 Signaling by moderate kinase activity BRAF mutants
R-HSA-6802949 Signaling by RAS mutants
R-HSA-6802955 Paradoxical activation of RAF signaling by kinase inactive BRAF
R-HSA-8949664 Processing of SMDT1

Miscellaneous databases

GeneWikiiProhibitin
GenomeRNAii5245
PROiPR:P35232
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000167085 Expressed in 231 organ(s), highest expression level in mucosa of transverse colon
CleanExiHS_PHB
ExpressionAtlasiP35232 baseline and differential
GenevisibleiP35232 HS

Family and domain databases

CDDicd03401 SPFH_prohibitin, 1 hit
InterProiView protein in InterPro
IPR001107 Band_7
IPR036013 Band_7/SPFH_dom_sf
IPR000163 Prohibitin
PANTHERiPTHR23222 PTHR23222, 1 hit
PfamiView protein in Pfam
PF01145 Band_7, 1 hit
PRINTSiPR00679 PROHIBITIN
SMARTiView protein in SMART
SM00244 PHB, 1 hit
SUPFAMiSSF117892 SSF117892, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPHB_HUMAN
AccessioniPrimary (citable) accession number: P35232
Secondary accession number(s): B4DY47, Q4VBQ0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 7, 2018
This is version 184 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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