UniProtKB - P35232 (PHB_HUMAN)
Prohibitin
PHB
Functioni
GO - Molecular functioni
- complement component C3a binding Source: UniProtKB
- complement component C3b binding Source: UniProtKB
- enzyme binding Source: BHF-UCL
- histone deacetylase binding Source: BHF-UCL
- proteinase activated receptor binding Source: UniProtKB
- protein C-terminus binding Source: UniProtKB
- protein heterodimerization activity Source: UniProtKB
- transcription corepressor activity Source: BHF-UCL
GO - Biological processi
- activation of phospholipase C activity Source: UniProtKB
- activation of protein kinase C activity Source: UniProtKB
- antiviral innate immune response Source: UniProtKB
- B cell activation Source: UniProtKB
- CD40 signaling pathway Source: UniProtKB
- cellular response to interleukin-6 Source: BHF-UCL
- DNA biosynthetic process Source: UniProtKB-KW
- histone deacetylation Source: UniProtKB
- immunoglobulin production Source: UniProtKB
- interleukin-17 production Source: UniProtKB
- mitochondrial calcium ion transmembrane transport Source: Reactome
- mitochondrion organization Source: UniProtKB
- modulation by host of viral RNA genome replication Source: UniProtKB
- negative regulation of androgen receptor signaling pathway Source: BHF-UCL
- negative regulation of cell growth Source: BHF-UCL
- negative regulation of cell population proliferation Source: BHF-UCL
- negative regulation of ERK1 and ERK2 cascade Source: UniProtKB
- negative regulation of glucocorticoid receptor signaling pathway Source: BHF-UCL
- negative regulation of protein catabolic process Source: UniProtKB
- negative regulation of transcription, DNA-templated Source: UniProtKB
- negative regulation of transcription by competitive promoter binding Source: BHF-UCL
- negative regulation of transcription by RNA polymerase II Source: BHF-UCL
- osteoblast differentiation Source: UniProtKB
- positive regulation of cell death Source: ParkinsonsUK-UCL
- positive regulation of complement activation Source: UniProtKB
- positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
- positive regulation of gene expression Source: ParkinsonsUK-UCL
- positive regulation of G protein-coupled receptor signaling pathway Source: UniProtKB
- positive regulation of NIK/NF-kappaB signaling Source: UniProtKB
- positive regulation of protein kinase B signaling Source: UniProtKB
- positive regulation of smooth muscle cell proliferation Source: UniProtKB
- positive regulation of transcription, DNA-templated Source: UniProtKB
- progesterone receptor signaling pathway Source: BHF-UCL
- protein stabilization Source: UniProtKB
- regulation of apoptotic process Source: UniProtKB
- regulation of transcription, DNA-templated Source: UniProtKB
- RIG-I signaling pathway Source: UniProtKB
- signal transduction Source: UniProtKB
- T-helper 17 type immune response Source: UniProtKB
- viral entry into host cell Source: UniProtKB
Keywordsi
Biological process | DNA synthesis |
Enzyme and pathway databases
PathwayCommonsi | P35232 |
Reactomei | R-HSA-5673000, RAF activation R-HSA-6802946, Signaling by moderate kinase activity BRAF mutants R-HSA-6802955, Paradoxical activation of RAF signaling by kinase inactive BRAF R-HSA-8949664, Processing of SMDT1 R-HSA-9649948, Signaling downstream of RAS mutants |
Names & Taxonomyi
Protein namesi | Recommended name: ProhibitinCurated |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:8912, PHB |
MIMi | 176705, gene |
neXtProti | NX_P35232 |
VEuPathDBi | HostDB:ENSG00000167085.11 |
Subcellular locationi
Plasma membrane
- Cell membrane 2 Publications
Nucleus
- Nucleus 3 Publications
Mitochondrion
- Mitochondrion inner membrane 2 Publications
Other locations
- Cytoplasm 2 Publications
Endosome
- early endosome Source: UniProtKB
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Mitochondrion
- mitochondrial inner membrane Source: UniProtKB
- mitochondrial prohibitin complex Source: UniProtKB
- mitochondrion Source: UniProtKB
Nucleus
- nucleoplasm Source: UniProtKB
- nucleus Source: UniProtKB
Plasma Membrane
- integral component of plasma membrane Source: HGNC-UCL
- plasma membrane Source: UniProtKB
Other locations
- cell surface Source: UniProtKB
- cytoplasm Source: UniProtKB
- membrane Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Membrane, Mitochondrion, Mitochondrion inner membrane, NucleusPathology & Biotechi
Keywords - Diseasei
Proto-oncogeneOrganism-specific databases
DisGeNETi | 5245 |
MalaCardsi | PHB |
OpenTargetsi | ENSG00000167085 |
PharmGKBi | PA33251 |
Miscellaneous databases
Pharosi | P35232, Tbio |
Chemistry databases
ChEMBLi | CHEMBL4295750 |
DrugBanki | DB15496, Didesmethylrocaglamide DB15495, Rocaglamide |
Genetic variation databases
BioMutai | PHB |
DMDMi | 464371 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000213878 | 2 – 272 | ProhibitinAdd BLAST | 271 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 | |
Modified residuei | 91 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 128 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 186 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 202 | N6-acetyllysine; alternateCombined sources | 1 | |
Modified residuei | 202 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 249 | PhosphotyrosineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | P35232 |
jPOSTi | P35232 |
MassIVEi | P35232 |
MaxQBi | P35232 |
PaxDbi | P35232 |
PeptideAtlasi | P35232 |
PRIDEi | P35232 |
ProteomicsDBi | 5497 54995 [P35232-1] |
TopDownProteomicsi | P35232-1 [P35232-1] |
2D gel databases
DOSAC-COBS-2DPAGEi | P35232 |
OGPi | P35232 |
REPRODUCTION-2DPAGEi | IPI00017334 P35232 |
SWISS-2DPAGEi | P35232 |
UCD-2DPAGEi | P35232 |
PTM databases
iPTMneti | P35232 |
PhosphoSitePlusi | P35232 |
SwissPalmi | P35232 |
Expressioni
Tissue specificityi
Developmental stagei
Inductioni
Gene expression databases
Bgeei | ENSG00000167085, Expressed in adult mammalian kidney and 245 other tissues |
ExpressionAtlasi | P35232, baseline and differential |
Genevisiblei | P35232, HS |
Organism-specific databases
HPAi | ENSG00000167085, Low tissue specificity |
Interactioni
Subunit structurei
The mitochondrial prohibitin complex consists of two subunits (PHB and PHB2), assembled into a membrane-associated ring-shaped supercomplex of approximately 1 mDa (PubMed:11302691, PubMed:20959514, PubMed:28017329, PubMed:31522117).
Interacts with STOML2 (PubMed:21746876).
Interacts with MAP1LC3B (membrane-bound form LC3-II); the interaction requires PHB2 and takes place upon Parkin-mediated mitochondrial damage (PubMed:28017329).
Interacts with STAT3 (unphosphorylated or phosphorylated at 'Ser-727') (PubMed:31899195).
Interacts with CLPB (PubMed:31522117).
Interacts with CD86 (via cytoplasmic domain); the interactions increases after priming with CD40 (By similarity).
By similarity6 Publications(Microbial infection) Interacts with SARS coronavirus/SARS-CoV nsp2 protein.
1 Publication(Microbial infection) Interacts with chikungunya virus spike glycoprotein E2.
1 Publication(Microbial infection) Interaction with human immunodeficiency virus type 1/HIV-1 envelope glycoprotein GP160.
1 Publication(Microbial infection) Interacts with human enterovirus 71/EV-71 capsid protein VP0, protein 3CD and protease 3C.
By similarityBinary interactionsi
Hide detailsP35232
GO - Molecular functioni
- complement component C3a binding Source: UniProtKB
- complement component C3b binding Source: UniProtKB
- enzyme binding Source: BHF-UCL
- histone deacetylase binding Source: BHF-UCL
- proteinase activated receptor binding Source: UniProtKB
- protein C-terminus binding Source: UniProtKB
- protein heterodimerization activity Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 111264, 974 interactors |
ComplexPortali | CPX-5741, Prohibitin complex |
CORUMi | P35232 |
DIPi | DIP-24248N |
IntActi | P35232, 143 interactors |
MINTi | P35232 |
STRINGi | 9606.ENSP00000479488 |
Miscellaneous databases
RNActi | P35232, protein |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 177 – 211 | Sequence analysisAdd BLAST | 35 |
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG3083, Eukaryota |
GeneTreei | ENSGT00950000183070 |
HOGENOMi | CLU_047969_0_0_1 |
InParanoidi | P35232 |
OMAi | RTRIRDN |
PhylomeDBi | P35232 |
TreeFami | TF300095 |
Family and domain databases
CDDi | cd03401, SPFH_prohibitin, 1 hit |
InterProi | View protein in InterPro IPR001107, Band_7 IPR036013, Band_7/SPFH_dom_sf IPR000163, Prohibitin |
PANTHERi | PTHR23222, PTHR23222, 1 hit |
Pfami | View protein in Pfam PF01145, Band_7, 1 hit |
PRINTSi | PR00679, PROHIBITIN |
SMARTi | View protein in SMART SM00244, PHB, 1 hit |
SUPFAMi | SSF117892, SSF117892, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAAKVFESIG KFGLALAVAG GVVNSALYNV DAGHRAVIFD RFRGVQDIVV
60 70 80 90 100
GEGTHFLIPW VQKPIIFDCR SRPRNVPVIT GSKDLQNVNI TLRILFRPVA
110 120 130 140 150
SQLPRIFTSI GEDYDERVLP SITTEILKSV VARFDAGELI TQRELVSRQV
160 170 180 190 200
SDDLTERAAT FGLILDDVSL THLTFGKEFT EAVEAKQVAQ QEAERARFVV
210 220 230 240 250
EKAEQQKKAA IISAEGDSKA AELIANSLAT AGDGLIELRK LEAAEDIAYQ
260 270
LSRSRNITYL PAGQSVLLQL PQ
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9JW96 | C9JW96_HUMAN | Prohibitin | PHB | 246 | Annotation score: | ||
E9PCW0 | E9PCW0_HUMAN | Prohibitin | PHB | 216 | Annotation score: | ||
C9JZ20 | C9JZ20_HUMAN | Prohibitin | PHB | 201 | Annotation score: | ||
E7ESE2 | E7ESE2_HUMAN | Prohibitin | PHB | 202 | Annotation score: | ||
D6RBK0 | D6RBK0_HUMAN | Prohibitin | PHB | 124 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_006479 | 88 | V → A in a breast cancer sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs121918373Ensembl. | 1 | |
Natural variantiVAR_006480 | 105 | R → H in a breast cancer sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs121918372Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_054922 | 140 – 256 | Missing in isoform 2. 1 PublicationAdd BLAST | 117 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S85655 mRNA Translation: AAB21614.1 L14272 Genomic DNA Translation: AAO18340.1 BT007411 mRNA Translation: AAP36079.1 AK302258 mRNA Translation: BAG63609.1 AC091180 Genomic DNA No translation available. BC013401 mRNA Translation: AAH13401.1 BC095460 mRNA Translation: AAH95460.1 |
CCDSi | CCDS11548.1 [P35232-1] CCDS62244.1 [P35232-2] |
PIRi | I52690 |
RefSeqi | NP_001268425.1, NM_001281496.1 [P35232-1] NP_001268426.1, NM_001281497.1 [P35232-2] NP_001268644.1, NM_001281715.1 [P35232-1] NP_002625.1, NM_002634.3 [P35232-1] XP_016880252.1, XM_017024763.1 [P35232-1] |
Genome annotation databases
Ensembli | ENST00000300408; ENSP00000300408; ENSG00000167085 [P35232-1] ENST00000511832; ENSP00000425035; ENSG00000167085 [P35232-2] ENST00000614445; ENSP00000479488; ENSG00000167085 [P35232-1] ENST00000617874; ENSP00000484113; ENSG00000167085 [P35232-1] |
GeneIDi | 5245 |
KEGGi | hsa:5245 |
UCSCi | uc002iox.3, human [P35232-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S85655 mRNA Translation: AAB21614.1 L14272 Genomic DNA Translation: AAO18340.1 BT007411 mRNA Translation: AAP36079.1 AK302258 mRNA Translation: BAG63609.1 AC091180 Genomic DNA No translation available. BC013401 mRNA Translation: AAH13401.1 BC095460 mRNA Translation: AAH95460.1 |
CCDSi | CCDS11548.1 [P35232-1] CCDS62244.1 [P35232-2] |
PIRi | I52690 |
RefSeqi | NP_001268425.1, NM_001281496.1 [P35232-1] NP_001268426.1, NM_001281497.1 [P35232-2] NP_001268644.1, NM_001281715.1 [P35232-1] NP_002625.1, NM_002634.3 [P35232-1] XP_016880252.1, XM_017024763.1 [P35232-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1LU7 | model | - | A | 1-272 | [»] | |
SMRi | P35232 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 111264, 974 interactors |
ComplexPortali | CPX-5741, Prohibitin complex |
CORUMi | P35232 |
DIPi | DIP-24248N |
IntActi | P35232, 143 interactors |
MINTi | P35232 |
STRINGi | 9606.ENSP00000479488 |
Chemistry databases
ChEMBLi | CHEMBL4295750 |
DrugBanki | DB15496, Didesmethylrocaglamide DB15495, Rocaglamide |
PTM databases
iPTMneti | P35232 |
PhosphoSitePlusi | P35232 |
SwissPalmi | P35232 |
Genetic variation databases
BioMutai | PHB |
DMDMi | 464371 |
2D gel databases
DOSAC-COBS-2DPAGEi | P35232 |
OGPi | P35232 |
REPRODUCTION-2DPAGEi | IPI00017334 P35232 |
SWISS-2DPAGEi | P35232 |
UCD-2DPAGEi | P35232 |
Proteomic databases
EPDi | P35232 |
jPOSTi | P35232 |
MassIVEi | P35232 |
MaxQBi | P35232 |
PaxDbi | P35232 |
PeptideAtlasi | P35232 |
PRIDEi | P35232 |
ProteomicsDBi | 5497 54995 [P35232-1] |
TopDownProteomicsi | P35232-1 [P35232-1] |
Protocols and materials databases
Antibodypediai | 1078, 696 antibodies |
DNASUi | 5245 |
Genome annotation databases
Ensembli | ENST00000300408; ENSP00000300408; ENSG00000167085 [P35232-1] ENST00000511832; ENSP00000425035; ENSG00000167085 [P35232-2] ENST00000614445; ENSP00000479488; ENSG00000167085 [P35232-1] ENST00000617874; ENSP00000484113; ENSG00000167085 [P35232-1] |
GeneIDi | 5245 |
KEGGi | hsa:5245 |
UCSCi | uc002iox.3, human [P35232-1] |
Organism-specific databases
CTDi | 5245 |
DisGeNETi | 5245 |
GeneCardsi | PHB |
HGNCi | HGNC:8912, PHB |
HPAi | ENSG00000167085, Low tissue specificity |
MalaCardsi | PHB |
MIMi | 176705, gene |
neXtProti | NX_P35232 |
OpenTargetsi | ENSG00000167085 |
PharmGKBi | PA33251 |
VEuPathDBi | HostDB:ENSG00000167085.11 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3083, Eukaryota |
GeneTreei | ENSGT00950000183070 |
HOGENOMi | CLU_047969_0_0_1 |
InParanoidi | P35232 |
OMAi | RTRIRDN |
PhylomeDBi | P35232 |
TreeFami | TF300095 |
Enzyme and pathway databases
PathwayCommonsi | P35232 |
Reactomei | R-HSA-5673000, RAF activation R-HSA-6802946, Signaling by moderate kinase activity BRAF mutants R-HSA-6802955, Paradoxical activation of RAF signaling by kinase inactive BRAF R-HSA-8949664, Processing of SMDT1 R-HSA-9649948, Signaling downstream of RAS mutants |
Miscellaneous databases
BioGRID-ORCSi | 5245, 768 hits in 884 CRISPR screens |
ChiTaRSi | PHB, human |
GeneWikii | Prohibitin |
GenomeRNAii | 5245 |
Pharosi | P35232, Tbio |
PROi | PR:P35232 |
RNActi | P35232, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000167085, Expressed in adult mammalian kidney and 245 other tissues |
ExpressionAtlasi | P35232, baseline and differential |
Genevisiblei | P35232, HS |
Family and domain databases
CDDi | cd03401, SPFH_prohibitin, 1 hit |
InterProi | View protein in InterPro IPR001107, Band_7 IPR036013, Band_7/SPFH_dom_sf IPR000163, Prohibitin |
PANTHERi | PTHR23222, PTHR23222, 1 hit |
Pfami | View protein in Pfam PF01145, Band_7, 1 hit |
PRINTSi | PR00679, PROHIBITIN |
SMARTi | View protein in SMART SM00244, PHB, 1 hit |
SUPFAMi | SSF117892, SSF117892, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PHB_HUMAN | |
Accessioni | P35232Primary (citable) accession number: P35232 Secondary accession number(s): B4DY47, Q4VBQ0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | February 1, 1994 | |
Last modified: | February 10, 2021 | |
This is version 203 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 17
Human chromosome 17: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families