UniProtKB - P35226 (BMI1_HUMAN)
Polycomb complex protein BMI-1
BMI1
Functioni
Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed:15386022, PubMed:16359901, PubMed:26151332, PubMed:16714294, PubMed:21772249, PubMed:25355358, PubMed:27827373).
The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A (PubMed:21772249, PubMed:25355358).
In the PRC1-like complex, regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2 (PubMed:15386022, PubMed:26151332, PubMed:21772249).
8 PublicationsMiscellaneous
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 18 – 57 | RING-typePROSITE-ProRule annotationAdd BLAST | 40 |
GO - Molecular functioni
- promoter-specific chromatin binding Source: UniProtKB
- RING-like zinc finger domain binding Source: UniProtKB
- zinc ion binding Source: UniProtKB
GO - Biological processi
- hemopoiesis Source: UniProtKB
- histone H2A-K119 monoubiquitination Source: UniProtKB
- negative regulation of gene expression, epigenetic Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: UniProtKB
- positive regulation of fibroblast proliferation Source: BHF-UCL
- positive regulation of ubiquitin-protein transferase activity Source: UniProtKB
- regulation of gene expression Source: BHF-UCL
- segment specification Source: ProtInc
Keywordsi
Molecular function | Chromatin regulator, Repressor |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | P35226 |
Reactomei | R-HSA-2559580, Oxidative Stress Induced Senescence R-HSA-3108214, SUMOylation of DNA damage response and repair proteins R-HSA-3899300, SUMOylation of transcription cofactors R-HSA-4551638, SUMOylation of chromatin organization proteins R-HSA-4570464, SUMOylation of RNA binding proteins R-HSA-4655427, SUMOylation of DNA methylation proteins R-HSA-8939243, RUNX1 interacts with co-factors whose precise effect on RUNX1 targets is not known R-HSA-8943724, Regulation of PTEN gene transcription R-HSA-8953750, Transcriptional Regulation by E2F6 |
SignaLinki | P35226 |
SIGNORi | P35226 |
Names & Taxonomyi
Protein namesi | Recommended name: Polycomb complex protein BMI-1Alternative name(s): Polycomb group RING finger protein 4 RING finger protein 51 |
Gene namesi | Name:BMI1 Synonyms:PCGF4, RNF51 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:1066, BMI1 |
MIMi | 164831, gene |
neXtProti | NX_P35226 |
VEuPathDBi | HostDB:ENSG00000168283 |
Subcellular locationi
Cytosol
- cytosol Source: HPA
Nucleus
- nuclear body Source: HPA
- nucleoplasm Source: Reactome
- nucleus Source: UniProtKB
- PcG protein complex Source: UniProtKB
- PRC1 complex Source: UniProtKB
Other locations
- ubiquitin ligase complex Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 62 | K → A: Strongly decreases histone H2A ubiquitination; when associated with A-64. 1 Publication | 1 | |
Mutagenesisi | 64 | R → A: Mildly decreases histone H2A ubiquitination. Strongly decreases histone H2A ubiquitination; when associated with A-62. 2 Publications | 1 | |
Mutagenesisi | 73 | K → N: Increases stimulation of RNF2 ubiquitin ligase activity; when associated with E-77. 1 Publication | 1 | |
Mutagenesisi | 77 | D → E: Increases stimulation of RNF2 ubiquitin ligase activity; when associated with N-73. 1 Publication | 1 | |
Mutagenesisi | 165 | R → E: Decreases affinity for PHC2. Abolishes interaction with PHC2; when associated with E-174. 1 Publication | 1 | |
Mutagenesisi | 170 | M → E: Strongly decreases affinity for PHC2. 1 Publication | 1 | |
Mutagenesisi | 174 | H → E: Strongly decreases affinity for PHC2. Abolishes interaction with PHC2; when associated with E-165. 1 Publication | 1 |
Keywords - Diseasei
Proto-oncogeneOrganism-specific databases
DisGeNETi | 100532731 648 |
OpenTargetsi | ENSG00000168283 |
PharmGKBi | PA25376 |
Miscellaneous databases
Pharosi | P35226, Tbio |
Chemistry databases
ChEMBLi | CHEMBL4295749 |
Genetic variation databases
BioMutai | BMI1 |
DMDMi | 22258801 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000055987 | 1 – 326 | Polycomb complex protein BMI-1Add BLAST | 326 |
Post-translational modificationi
Keywords - PTMi
Ubl conjugationProteomic databases
EPDi | P35226 |
jPOSTi | P35226 |
MassIVEi | P35226 |
MaxQBi | P35226 |
PaxDbi | P35226 |
PeptideAtlasi | P35226 |
PRIDEi | P35226 |
ProteomicsDBi | 54991 |
PTM databases
GlyGeni | P35226, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | P35226 |
PhosphoSitePlusi | P35226 |
Expressioni
Inductioni
Gene expression databases
Bgeei | ENSG00000168283, Expressed in brain and 247 other tissues |
ExpressionAtlasi | P35226, baseline and differential |
Genevisiblei | P35226, HS |
Organism-specific databases
HPAi | ENSG00000168283, Low tissue specificity |
Interactioni
Subunit structurei
Component of a PRC1-like complex (PubMed:12167701, PubMed:15386022, PubMed:19636380, PubMed:21282530, PubMed:26151332, PubMed:21772249, PubMed:25355358).
Identified in a PRC1-like HPRC-H complex with CBX2, CBX4, CBX8, PHC1, PHC2, PHC3 RING1 and RNF2 (PubMed:12167701).
Interacts with RNF2/RING2 (PubMed:16714294, PubMed:21772249, PubMed:25355358).
Interacts with RING1 (By similarity).
Part of a complex that contains RNF2, UB2D3 and BMI1, where RNF2 and BMI1 form a tight heterodimer, and UB2D3 interacts only with RNF2 (PubMed:21772249, PubMed:25355358). The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A (PubMed:21772249, PubMed:25355358).
Interacts with CBX7 and CBX8 (PubMed:19636380).
Interacts with SPOP (PubMed:15897469).
Part of a complex consisting of BMI1, CUL3 and SPOP (PubMed:15897469).
Interacts with E4F1 (PubMed:16882984).
Interacts with PHC2 (PubMed:9121482, PubMed:9199346, PubMed:27827373).
Interacts with zinc finger protein ZNF277 (By similarity). May be part of a complex including at least ZNF277, BMI1 and RNF2/RING2 (By similarity).
By similarity13 PublicationsBinary interactionsi
P35226
GO - Molecular functioni
- RING-like zinc finger domain binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 107116, 477 interactors 1529410, 16 interactors |
CORUMi | P35226 |
DIPi | DIP-41879N |
IntActi | P35226, 961 interactors |
MINTi | P35226 |
STRINGi | 9606.ENSP00000365851 |
Miscellaneous databases
RNActi | P35226, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P35226 |
SMRi | P35226 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P35226 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 162 – 182 | Interaction with PHC21 PublicationAdd BLAST | 21 | |
Regioni | 164 – 228 | Interaction with E4F11 PublicationAdd BLAST | 65 | |
Regioni | 236 – 326 | DisorderedSequence analysisAdd BLAST | 91 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 81 – 95 | Nuclear localization signalSequence analysisAdd BLAST | 15 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 244 – 276 | Polar residuesSequence analysisAdd BLAST | 33 | |
Compositional biasi | 283 – 326 | Polar residuesSequence analysisAdd BLAST | 44 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 18 – 57 | RING-typePROSITE-ProRule annotationAdd BLAST | 40 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG2660, Eukaryota |
GeneTreei | ENSGT00940000156042 |
HOGENOMi | CLU_046427_0_0_1 |
InParanoidi | P35226 |
OMAi | DIDARYL |
OrthoDBi | 1344247at2759 |
PhylomeDBi | P35226 |
TreeFami | TF324206 |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
IDEALi | IID00450 |
InterProi | View protein in InterPro IPR032443, RAWUL IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
Pfami | View protein in Pfam PF16207, RAWUL, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MHRTTRIKIT ELNPHLMCVL CGGYFIDATT IIECLHSFCK TCIVRYLETS
60 70 80 90 100
KYCPICDVQV HKTRPLLNIR SDKTLQDIVY KLVPGLFKNE MKRRRDFYAA
110 120 130 140 150
HPSADAANGS NEDRGEVADE DKRIITDDEI ISLSIEFFDQ NRLDRKVNKD
160 170 180 190 200
KEKSKEEVND KRYLRCPAAM TVMHLRKFLR SKMDIPNTFQ IDVMYEEEPL
210 220 230 240 250
KDYYTLMDIA YIYTWRRNGP LPLKYRVRPT CKRMKISHQR DGLTNAGELE
260 270 280 290 300
SDSGSDKANS PAGGIPSTSS CLPSPSTPVQ SPHPQFPHIS STMNGTSNSP
310 320
SGNHQSSFAN RPRKSSVNGS SATSSG
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ5T8Z2 | Q5T8Z2_HUMAN | Polycomb complex protein BMI-1 | BMI1 | 184 | Annotation score: | ||
Q5T8Z4 | Q5T8Z4_HUMAN | Polycomb complex protein BMI-1 | BMI1 | 150 | Annotation score: | ||
Q5T8Z6 | Q5T8Z6_HUMAN | Polycomb complex protein BMI-1 | BMI1 | 105 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 109 | G → S in AAB27059 (PubMed:8390036).Curated | 1 | |
Sequence conflicti | 265 | I → V in AAA19873 (PubMed:8268912).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_052087 | 18 | C → Y. Corresponds to variant dbSNP:rs1042059Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L13689 mRNA Translation: AAA19873.1 AK313235 mRNA Translation: BAG36046.1 AL158211 Genomic DNA No translation available. CH471072 Genomic DNA Translation: EAW86148.1 CH471072 Genomic DNA Translation: EAW86150.1 CH471072 Genomic DNA Translation: EAW86151.1 CH471072 Genomic DNA Translation: EAW86154.1 BC011652 mRNA Translation: AAH11652.1 AH004292 mRNA Translation: AAB27059.1 |
CCDSi | CCDS7138.1 |
PIRi | I54339 |
RefSeqi | NP_001190991.1, NM_001204062.1 NP_005171.4, NM_005180.8 |
Genome annotation databases
Ensembli | ENST00000376663.8; ENSP00000365851.3; ENSG00000168283.14 |
GeneIDi | 100532731 648 |
KEGGi | hsa:100532731 hsa:648 |
MANE-Selecti | ENST00000376663.8; ENSP00000365851.3; NM_005180.9; NP_005171.4 |
UCSCi | uc001irh.4, human |
Similar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L13689 mRNA Translation: AAA19873.1 AK313235 mRNA Translation: BAG36046.1 AL158211 Genomic DNA No translation available. CH471072 Genomic DNA Translation: EAW86148.1 CH471072 Genomic DNA Translation: EAW86150.1 CH471072 Genomic DNA Translation: EAW86151.1 CH471072 Genomic DNA Translation: EAW86154.1 BC011652 mRNA Translation: AAH11652.1 AH004292 mRNA Translation: AAB27059.1 |
CCDSi | CCDS7138.1 |
PIRi | I54339 |
RefSeqi | NP_001190991.1, NM_001204062.1 NP_005171.4, NM_005180.8 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2H0D | X-ray | 2.50 | A | 5-101 | [»] | |
2NA1 | NMR | - | A | 121-235 | [»] | |
3RPG | X-ray | 2.65 | B | 1-109 | [»] | |
4R8P | X-ray | 3.28 | K/M | 2-109 | [»] | |
5FR6 | X-ray | 2.51 | A | 121-235 | [»] | |
6WI7 | X-ray | 1.70 | A | 1-104 | [»] | |
6WI8 | X-ray | 3.09 | A/B | 1-104 | [»] | |
7ND1 | NMR | - | H | 4-104 | [»] | |
AlphaFoldDBi | P35226 | |||||
SMRi | P35226 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 107116, 477 interactors 1529410, 16 interactors |
CORUMi | P35226 |
DIPi | DIP-41879N |
IntActi | P35226, 961 interactors |
MINTi | P35226 |
STRINGi | 9606.ENSP00000365851 |
Chemistry databases
ChEMBLi | CHEMBL4295749 |
PTM databases
GlyGeni | P35226, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | P35226 |
PhosphoSitePlusi | P35226 |
Genetic variation databases
BioMutai | BMI1 |
DMDMi | 22258801 |
Proteomic databases
EPDi | P35226 |
jPOSTi | P35226 |
MassIVEi | P35226 |
MaxQBi | P35226 |
PaxDbi | P35226 |
PeptideAtlasi | P35226 |
PRIDEi | P35226 |
ProteomicsDBi | 54991 |
Protocols and materials databases
Antibodypediai | 4554, 942 antibodies from 46 providers |
DNASUi | 648 |
Genome annotation databases
Ensembli | ENST00000376663.8; ENSP00000365851.3; ENSG00000168283.14 |
GeneIDi | 100532731 648 |
KEGGi | hsa:100532731 hsa:648 |
MANE-Selecti | ENST00000376663.8; ENSP00000365851.3; NM_005180.9; NP_005171.4 |
UCSCi | uc001irh.4, human |
Organism-specific databases
CTDi | 100532731 648 |
DisGeNETi | 100532731 648 |
GeneCardsi | BMI1 |
HGNCi | HGNC:1066, BMI1 |
HPAi | ENSG00000168283, Low tissue specificity |
MIMi | 164831, gene |
neXtProti | NX_P35226 |
OpenTargetsi | ENSG00000168283 |
PharmGKBi | PA25376 |
VEuPathDBi | HostDB:ENSG00000168283 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2660, Eukaryota |
GeneTreei | ENSGT00940000156042 |
HOGENOMi | CLU_046427_0_0_1 |
InParanoidi | P35226 |
OMAi | DIDARYL |
OrthoDBi | 1344247at2759 |
PhylomeDBi | P35226 |
TreeFami | TF324206 |
Enzyme and pathway databases
PathwayCommonsi | P35226 |
Reactomei | R-HSA-2559580, Oxidative Stress Induced Senescence R-HSA-3108214, SUMOylation of DNA damage response and repair proteins R-HSA-3899300, SUMOylation of transcription cofactors R-HSA-4551638, SUMOylation of chromatin organization proteins R-HSA-4570464, SUMOylation of RNA binding proteins R-HSA-4655427, SUMOylation of DNA methylation proteins R-HSA-8939243, RUNX1 interacts with co-factors whose precise effect on RUNX1 targets is not known R-HSA-8943724, Regulation of PTEN gene transcription R-HSA-8953750, Transcriptional Regulation by E2F6 |
SignaLinki | P35226 |
SIGNORi | P35226 |
Miscellaneous databases
BioGRID-ORCSi | 100532731, 17 hits in 606 CRISPR screens 648, 44 hits in 1113 CRISPR screens |
EvolutionaryTracei | P35226 |
GeneWikii | BMI1 |
Pharosi | P35226, Tbio |
PROi | PR:P35226 |
RNActi | P35226, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000168283, Expressed in brain and 247 other tissues |
ExpressionAtlasi | P35226, baseline and differential |
Genevisiblei | P35226, HS |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
IDEALi | IID00450 |
InterProi | View protein in InterPro IPR032443, RAWUL IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
Pfami | View protein in Pfam PF16207, RAWUL, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | BMI1_HUMAN | |
Accessioni | P35226Primary (citable) accession number: P35226 Secondary accession number(s): Q16030, Q5T8Z3, Q96F37 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | August 13, 2002 | |
Last modified: | May 25, 2022 | |
This is version 216 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 10
Human chromosome 10: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references