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Protein

Thiamine pyrophosphokinase

Gene

THI80

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential protein, it is the only enzyme in yeast capable of synthesizing thiamine pyrophosphate (TPP).

Miscellaneous

Present with 3320 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + thiamine = AMP + thiamine diphosphate.

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes thiamine diphosphate from thiamine.
Proteins known to be involved in this subpathway in this organism are:
  1. Thiamine pyrophosphokinase (THI80)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes thiamine diphosphate from thiamine, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • kinase activity Source: UniProtKB-KW
  • thiamine binding Source: InterPro
  • thiamine diphosphokinase activity Source: SGD

GO - Biological processi

  • thiamine diphosphate biosynthetic process Source: SGD

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:YOR143C-MONOMER
YEAST:YOR143C-MONOMER
ReactomeiR-SCE-196819 Vitamin B1 (thiamin) metabolism
UniPathwayiUPA00060; UER00597

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine pyrophosphokinase (EC:2.7.6.2)
Short name:
TPK
Short name:
Thiamine kinase
Gene namesi
Name:THI80
Ordered Locus Names:YOR143C
ORF Names:YOR3373C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR143C
SGDiS000005669 THI80

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000725132 – 319Thiamine pyrophosphokinaseAdd BLAST318

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP35202
PaxDbiP35202
PRIDEiP35202

PTM databases

iPTMnetiP35202

Expressioni

Inductioni

Expression requires the positive regulatory factors THI2 and THI3. Incompletely repressed by exogenous thiamine pyrophosphokinase.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi34540, 152 interactors
DIPiDIP-5324N
IntActiP35202, 6 interactors
STRINGi4932.YOR143C

Structurei

Secondary structure

1319
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 7Combined sources4
Beta strandi10 – 12Combined sources3
Beta strandi23 – 27Combined sources5
Helixi28 – 30Combined sources3
Beta strandi37 – 43Combined sources7
Helixi52 – 61Combined sources10
Beta strandi63 – 68Combined sources6
Helixi71 – 78Combined sources8
Helixi83 – 86Combined sources4
Beta strandi92 – 96Combined sources5
Helixi103 – 111Combined sources9
Beta strandi115 – 118Combined sources4
Beta strandi122 – 124Combined sources3
Helixi126 – 139Combined sources14
Helixi141 – 147Combined sources7
Turni153 – 155Combined sources3
Helixi156 – 158Combined sources3
Turni161 – 163Combined sources3
Helixi164 – 173Combined sources10
Helixi178 – 180Combined sources3
Beta strandi184 – 195Combined sources12
Helixi196 – 212Combined sources17
Beta strandi216 – 221Combined sources6
Beta strandi223 – 230Combined sources8
Beta strandi235 – 238Combined sources4
Helixi241 – 247Combined sources7
Beta strandi248 – 254Combined sources7
Beta strandi260 – 278Combined sources19
Turni279 – 282Combined sources4
Beta strandi285 – 288Combined sources4
Beta strandi292 – 302Combined sources11
Beta strandi304 – 310Combined sources7
Helixi312 – 315Combined sources4
Helixi316 – 318Combined sources3

3D structure databases

ProteinModelPortaliP35202
SMRiP35202
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP35202

Family & Domainsi

Sequence similaritiesi

Belongs to the thiamine pyrophosphokinase family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000016016
HOGENOMiHOG000248374
InParanoidiP35202
KOiK00949
OMAiHEIRIER
OrthoDBiEOG092C5171

Family and domain databases

CDDicd07995 TPK, 1 hit
Gene3Di3.40.50.10240, 1 hit
InterProiView protein in InterPro
IPR016966 Thiamin_pyrophosphokinase_euk
IPR007373 Thiamin_PyroPKinase_B1-bd
IPR036371 TPK_B1-bd_sf
IPR007371 TPK_catalytic
IPR036759 TPK_catalytic_sf
PfamiView protein in Pfam
PF04265 TPK_B1_binding, 1 hit
PF04263 TPK_catalytic, 1 hit
PIRSFiPIRSF031057 Thiamin_pyrophosphokinase, 1 hit
SMARTiView protein in SMART
SM00983 TPK_B1_binding, 1 hit
SUPFAMiSSF63862 SSF63862, 1 hit
SSF63999 SSF63999, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35202-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEECIENPE RIKIGTDLIN IRNKMNLKEL IHPNEDENST LLILNQKIDI
60 70 80 90 100
PRPLFYKIWK LHDLKVCADG AANRLYDYLD DDETLRIKYL PNYIIGDLDS
110 120 130 140 150
LSEKVYKYYR KNKVTIIKQT TQYSTDFTKC VNLISLHFNS PEFRSLISNK
160 170 180 190 200
DNLQSNHGIE LEKGIHTLYN TMTESLVFSK VTPISLLALG GIGGRFDQTV
210 220 230 240 250
HSITQLYTLS ENASYFKLCY MTPTDLIFLI KKNGTLIEYD PQFRNTCIGN
260 270 280 290 300
CGLLPIGEAT LVKETRGLKW DVKNWPTSVV TGRVSSSNRF VGDNCCFIDT
310
KDDIILNVEI FVDKLIDFL
Length:319
Mass (Da):36,616
Last modified:February 1, 1994 - v1
Checksum:iB82609E195D4D53F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14417 Genomic DNA Translation: BAA03312.1
X94335 Genomic DNA Translation: CAA64061.1
Z75051 Genomic DNA Translation: CAA99346.1
BK006948 Genomic DNA Translation: DAA10917.1
PIRiA47499
RefSeqiNP_014786.1, NM_001183562.1

Genome annotation databases

EnsemblFungiiYOR143C; YOR143C; YOR143C
GeneIDi854314
KEGGisce:YOR143C

Similar proteinsi

Entry informationi

Entry nameiTHI80_YEAST
AccessioniPrimary (citable) accession number: P35202
Secondary accession number(s): D6W2K1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: July 18, 2018
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

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