UniProtKB - P35201 (CENPC_YEAST)
Protein
Inner kinetochore subunit MIF2
Gene
MIF2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly (PubMed:8408221, PubMed:7579695, PubMed:22561346).3 Publications
Miscellaneous
Present with 465 molecules/cell in log phase SD medium.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 356 – 364 | A.T hook | 9 |
GO - Molecular functioni
- centromeric DNA binding Source: SGD
- protein-containing complex binding Source: SGD
GO - Biological processi
- attachment of mitotic spindle microtubules to kinetochore Source: GO_Central
- cell division Source: UniProtKB-KW
- chromosome segregation Source: SGD
- kinetochore assembly Source: SGD
- mitotic spindle organization Source: SGD
- monopolar spindle attachment to meiosis I kinetochore Source: GO_Central
Keywordsi
Molecular function | DNA-binding |
Biological process | Cell cycle, Cell division, Mitosis |
Names & Taxonomyi
Protein namesi | Recommended name: Inner kinetochore subunit MIF2CuratedAlternative name(s): CENP-C homolog1 Publication Constitutive centromere-associated network protein MIF2Curated Mitotic fidelity of chromosome transmission protein 21 Publication |
Gene namesi | Name:MIF2 Ordered Locus Names:YKL089W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YKL089W |
SGDi | S000001572, MIF2 |
Subcellular locationi
Nucleus
Other locations
- centromere
- kinetochore 1 Publication
Nucleus
- condensed nuclear chromosome kinetochore Source: GO_Central
- condensed nuclear chromosome, centromeric region Source: SGD
Keywords - Cellular componenti
Centromere, Chromosome, Kinetochore, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000096486 | 1 – 549 | Inner kinetochore subunit MIF2Add BLAST | 549 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 325 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P35201 |
PaxDbi | P35201 |
PRIDEi | P35201 |
PTM databases
iPTMneti | P35201 |
Interactioni
Subunit structurei
Component of the inner kinetochore constitutive centromere-associated network (CCAN) (also known as central kinetochore CTF19 complex in yeast), which is composed of at least AME1, CHL4, CNN1, CTF3, CTF19, IML3, MCM16, MCM21, MCM22, MHF1, MHF2, MIF2, NKP1, NKP2, OKP1 and WIP1 (PubMed:22561346).
Interacts with CBF1 (PubMed:7579695).
2 PublicationsProtein-protein interaction databases
BioGRIDi | 34044, 289 interactors |
DIPi | DIP-7297N |
IntActi | P35201, 4 interactors |
MINTi | P35201 |
STRINGi | 4932.YKL089W |
Miscellaneous databases
RNActi | P35201, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P35201 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P35201 |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 178 – 263 | Asp/Glu-rich (acidic)Add BLAST | 86 |
Sequence similaritiesi
Belongs to the CENP-C/MIF2 family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | ENOG502S47H, Eukaryota |
GeneTreei | ENSGT00390000016737 |
HOGENOMi | CLU_038087_0_0_1 |
InParanoidi | P35201 |
OMAi | FWRNERI |
Family and domain databases
Gene3Di | 2.60.120.10, 1 hit |
InterProi | View protein in InterPro IPR028386, CENP-C/Mif2/cnp3 IPR025974, Mif2/CENP-C_cupin IPR028929, Mif2/cnp3_N IPR014710, RmlC-like_jellyroll |
PANTHERi | PTHR16684, PTHR16684, 1 hit |
Pfami | View protein in Pfam PF11699, CENP-C_C, 1 hit PF15624, Mif2_N, 1 hit |
i Sequence
Sequence statusi: Complete.
P35201-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDYMKLGLKS RKTGIDVKQD IPKDEYSMEN IDDFFKDDET SLISMRRKSR
60 70 80 90 100
RKSSLFLPST LNGDTKNVLP PFLQSYKSQD DEVVQSPSGK GDGSRRSSLL
110 120 130 140 150
SHQSNFLSPA NDFEPIEEEP EQEENDIRGN DFATPITQKL SKPTYKRKYS
160 170 180 190 200
TRYSLDTSES PSVRLTPDRI TNKNVYSDVP DLVADEDDDD RVNTSLNTSD
210 220 230 240 250
NALLEDELED DGFIPESEED GDYIESDSSL DSGSDSASDS DGDNTYQEVE
260 270 280 290 300
EEAEVNTNDN EDDYIRRQAS DVVRTDSIID RNGLRKSTRV KVAPLQYWRN
310 320 330 340 350
EKIVYKRKSN KPVLDIDKIV TYDESEDEEE ILAAQRRKKQ KKKPTPTRPY
360 370 380 390 400
NYVPTGRPRG RPKKDPNAKE NLIPEDPNED IIERIESGGI ENGEWLKHGI
410 420 430 440 450
LEANVKISDT KEETKDEIIA FAPNLSQTEQ VKDTKDENFA LEIMFDKHKE
460 470 480 490 500
YFASGILKLP AISGQKKLSN SFRTYITFHV IQGIVEVTVC KNKFLSVKGS
510 520 530 540
TFQIPAFNEY AIANRGNDEA KMFFVQVTVS EDANDDNDKE LDSTFDTFG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z18294 Genomic DNA Translation: CAA79163.1 Z28089 Genomic DNA Translation: CAA81927.1 BK006944 Genomic DNA Translation: DAA09069.1 |
PIRi | S37914 |
RefSeqi | NP_012834.1, NM_001179655.1 |
Genome annotation databases
EnsemblFungii | YKL089W_mRNA; YKL089W; YKL089W |
GeneIDi | 853773 |
KEGGi | sce:YKL089W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z18294 Genomic DNA Translation: CAA79163.1 Z28089 Genomic DNA Translation: CAA81927.1 BK006944 Genomic DNA Translation: DAA09069.1 |
PIRi | S37914 |
RefSeqi | NP_012834.1, NM_001179655.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2VPV | X-ray | 2.70 | A/B | 365-530 | [»] | |
6QLD | electron microscopy | 4.15 | C | 284-305 | [»] | |
SMRi | P35201 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 34044, 289 interactors |
DIPi | DIP-7297N |
IntActi | P35201, 4 interactors |
MINTi | P35201 |
STRINGi | 4932.YKL089W |
PTM databases
iPTMneti | P35201 |
Proteomic databases
MaxQBi | P35201 |
PaxDbi | P35201 |
PRIDEi | P35201 |
Genome annotation databases
EnsemblFungii | YKL089W_mRNA; YKL089W; YKL089W |
GeneIDi | 853773 |
KEGGi | sce:YKL089W |
Organism-specific databases
EuPathDBi | FungiDB:YKL089W |
SGDi | S000001572, MIF2 |
Phylogenomic databases
eggNOGi | ENOG502S47H, Eukaryota |
GeneTreei | ENSGT00390000016737 |
HOGENOMi | CLU_038087_0_0_1 |
InParanoidi | P35201 |
OMAi | FWRNERI |
Miscellaneous databases
EvolutionaryTracei | P35201 |
PROi | PR:P35201 |
RNActi | P35201, protein |
Family and domain databases
Gene3Di | 2.60.120.10, 1 hit |
InterProi | View protein in InterPro IPR028386, CENP-C/Mif2/cnp3 IPR025974, Mif2/CENP-C_cupin IPR028929, Mif2/cnp3_N IPR014710, RmlC-like_jellyroll |
PANTHERi | PTHR16684, PTHR16684, 1 hit |
Pfami | View protein in Pfam PF11699, CENP-C_C, 1 hit PF15624, Mif2_N, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CENPC_YEAST | |
Accessioni | P35201Primary (citable) accession number: P35201 Secondary accession number(s): D6VXJ9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | February 1, 1994 | |
Last modified: | December 2, 2020 | |
This is version 170 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast chromosome XI
Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD