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UniProtKB - P35197 (GCS1_YEAST)
Protein
ADP-ribosylation factor GTPase-activating protein GCS1
Gene
GCS1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
GTPase-activating protein (GAP) for ARF1 and ARF2. Involved in intracellular vesicular transport. Required for transport from the trans-Golgi network. Implicated in the regulation of retrograde transport from the Golgi to the ER and in actin cytoskeletal organization. May be involved in the maintenance of mitochondrial morphology, possibly through organizing the actin cytoskeleton in Saccharomyces.
3 PublicationsMiscellaneous
Present with 9560 molecules/cell in log phase SD medium.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 26 – 49 | C4-typePROSITE-ProRule annotationAdd BLAST | 24 |
GO - Molecular functioni
- actin binding Source: SGD
- GTPase activator activity Source: SGD
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- actin filament reorganization involved in cell cycle Source: SGD
- endoplasmic reticulum to Golgi vesicle-mediated transport Source: SGD
- Golgi to plasma membrane protein transport Source: SGD
- retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum Source: SGD
Keywordsi
Molecular function | GTPase activation |
Biological process | Protein transport, Transport |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-SCE-6807878, COPI-mediated anterograde transport R-SCE-6811434, COPI-dependent Golgi-to-ER retrograde traffic |
Names & Taxonomyi
Protein namesi | Recommended name: ADP-ribosylation factor GTPase-activating protein GCS1Short name: ARF GAP GCS1 |
Gene namesi | Name:GCS1 Ordered Locus Names:YDL226C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000002385, GCS1 |
VEuPathDBi | FungiDB:YDL226C |
Subcellular locationi
Golgi apparatus
- Golgi apparatus 1 Publication
Mitochondrion
- Mitochondrion 1 Publication
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Other locations
- perinuclear region 1 Publication
Note: Found also in the mitochondria and in the perinuclear region.
Cytoskeleton
- cytoskeleton Source: SGD
Cytosol
- cytosol Source: SGD
Endosome
- endosome Source: SGD
Golgi apparatus
- trans-Golgi network Source: SGD
Mitochondrion
- mitochondrion Source: UniProtKB-SubCell
Other locations
- perinuclear region of cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cytoplasm, Golgi apparatus, MitochondrionPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 29 | C → Y in GCS1-1; severely affect the function. | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000074224 | 1 – 352 | ADP-ribosylation factor GTPase-activating protein GCS1Add BLAST | 352 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 151 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 157 | PhosphoserineCombined sources | 1 | |
Modified residuei | 161 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 168 | PhosphoserineCombined sources | 1 | |
Modified residuei | 170 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 260 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P35197 |
PaxDbi | P35197 |
PRIDEi | P35197 |
PTM databases
iPTMneti | P35197 |
Interactioni
GO - Molecular functioni
- actin binding Source: SGD
Protein-protein interaction databases
BioGRIDi | 31884, 260 interactors |
DIPi | DIP-809N |
IntActi | P35197, 17 interactors |
MINTi | P35197 |
STRINGi | 4932.YDL226C |
Miscellaneous databases
RNActi | P35197, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 11 – 127 | Arf-GAPPROSITE-ProRule annotationAdd BLAST | 117 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 138 – 181 | DisorderedSequence analysisAdd BLAST | 44 | |
Regioni | 196 – 231 | DisorderedSequence analysisAdd BLAST | 36 | |
Regioni | 315 – 352 | DisorderedSequence analysisAdd BLAST | 38 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 315 – 332 | Polar residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 333 – 352 | Basic and acidic residuesSequence analysisAdd BLAST | 20 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 26 – 49 | C4-typePROSITE-ProRule annotationAdd BLAST | 24 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG0704, Eukaryota |
HOGENOMi | CLU_044516_2_0_1 |
InParanoidi | P35197 |
OMAi | NVCCDCN |
Family and domain databases
Gene3Di | 1.10.220.150, 1 hit |
InterProi | View protein in InterPro IPR037278, ARFGAP/RecO IPR001164, ArfGAP_dom IPR038508, ArfGAP_dom_sf |
Pfami | View protein in Pfam PF01412, ArfGap, 1 hit |
PRINTSi | PR00405, REVINTRACTNG |
SMARTi | View protein in SMART SM00105, ArfGap, 1 hit |
SUPFAMi | SSF57863, SSF57863, 1 hit |
PROSITEi | View protein in PROSITE PS50115, ARFGAP, 1 hit |
i Sequence
Sequence statusi: Complete.
P35197-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSDWKVDPDT RRRLLQLQKI GANKKCMDCG APNPQWATPK FGAFICLECA
60 70 80 90 100
GIHRGLGVHI SFVRSITMDQ FKPEELLRME KGGNEPLTEW FKSHNIDLSL
110 120 130 140 150
PQKVKYDNPV AEDYKEKLTC LCEDRVFEER EHLDFDASKL SATSQTAASA
160 170 180 190 200
TPGVAQSREG TPLENRRSAT PANSSNGANF QKEKNEAYFA ELGKKNQSRP
210 220 230 240 250
DHLPPSQGGK YQGFGSTPAK PPQERSAGSS NTLSLENFQA DPLGTLSRGW
260 270 280 290 300
GLFSSAVTKS FEDVNETVIK PHVQQWQSGE LSEETKRAAA QFGQKFQETS
310 320 330 340 350
SYGFQAFSNF TKNFNGNAED SSTAGNTTHT EYQKIDNNDK KNEQDEDKWD
DF
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 27 | M → I. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L24125 Genomic DNA Translation: AAA50389.1 Z74274 Genomic DNA Translation: CAA98805.1 BK006938 Genomic DNA Translation: DAA11639.1 |
PIRi | S47006 |
RefSeqi | NP_010055.1, NM_001180286.1 |
Genome annotation databases
EnsemblFungii | YDL226C_mRNA; YDL226C; YDL226C |
GeneIDi | 851372 |
KEGGi | sce:YDL226C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L24125 Genomic DNA Translation: AAA50389.1 Z74274 Genomic DNA Translation: CAA98805.1 BK006938 Genomic DNA Translation: DAA11639.1 |
PIRi | S47006 |
RefSeqi | NP_010055.1, NM_001180286.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5FJX | X-ray | 2.45 | D/E | 345-352 | [»] | |
SMRi | P35197 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 31884, 260 interactors |
DIPi | DIP-809N |
IntActi | P35197, 17 interactors |
MINTi | P35197 |
STRINGi | 4932.YDL226C |
PTM databases
iPTMneti | P35197 |
Proteomic databases
MaxQBi | P35197 |
PaxDbi | P35197 |
PRIDEi | P35197 |
Genome annotation databases
EnsemblFungii | YDL226C_mRNA; YDL226C; YDL226C |
GeneIDi | 851372 |
KEGGi | sce:YDL226C |
Organism-specific databases
SGDi | S000002385, GCS1 |
VEuPathDBi | FungiDB:YDL226C |
Phylogenomic databases
eggNOGi | KOG0704, Eukaryota |
HOGENOMi | CLU_044516_2_0_1 |
InParanoidi | P35197 |
OMAi | NVCCDCN |
Enzyme and pathway databases
Reactomei | R-SCE-6807878, COPI-mediated anterograde transport R-SCE-6811434, COPI-dependent Golgi-to-ER retrograde traffic |
Miscellaneous databases
PROi | PR:P35197 |
RNActi | P35197, protein |
Family and domain databases
Gene3Di | 1.10.220.150, 1 hit |
InterProi | View protein in InterPro IPR037278, ARFGAP/RecO IPR001164, ArfGAP_dom IPR038508, ArfGAP_dom_sf |
Pfami | View protein in Pfam PF01412, ArfGap, 1 hit |
PRINTSi | PR00405, REVINTRACTNG |
SMARTi | View protein in SMART SM00105, ArfGap, 1 hit |
SUPFAMi | SSF57863, SSF57863, 1 hit |
PROSITEi | View protein in PROSITE PS50115, ARFGAP, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GCS1_YEAST | |
Accessioni | P35197Primary (citable) accession number: P35197 Secondary accession number(s): D6VRC9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | February 1, 1994 | |
Last modified: | February 23, 2022 | |
This is version 192 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references