UniProtKB - P35192 (MAC1_YEAST)
Protein
Metal-binding activator 1
Gene
MAC1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Regulatory protein involved in Cu/Fe utilization and stress resistance. Involved in basal level transcription of FRE1 and H2O2-induced transcription of CTT1. Regulates the transcription of CTR1 and CTR3 via the copper ion responsive elements in their promoters. Required for degradation of CTR1.1 Publication
Miscellaneous
Present with 14800 molecules/cell in log phase SD medium.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 11 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 14 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 23 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 25 | ZincPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 1 – 40 | Copper-fistPROSITE-ProRule annotationAdd BLAST | 40 |
GO - Molecular functioni
- copper ion binding Source: GO_Central
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: SGD
- proximal promoter sequence-specific DNA binding Source: SGD
- RNA polymerase II proximal promoter sequence-specific DNA binding Source: GO_Central
GO - Biological processi
- cellular copper ion homeostasis Source: SGD
- cellular iron ion homeostasis Source: GO_Central
- positive regulation of protein catabolic process Source: SGD
- positive regulation of transcription by RNA polymerase II Source: SGD
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Ligand | Copper, Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | YEAST:G3O-32726-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Metal-binding activator 1 |
Gene namesi | Name:MAC1 Synonyms:CUA1 Ordered Locus Names:YMR021C ORF Names:YM9711.11C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YMR021C |
SGDi | S000004623 MAC1 |
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 279 | H → Q: Gain of function. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000194928 | 1 – 417 | Metal-binding activator 1Add BLAST | 417 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 143 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P35192 |
PaxDbi | P35192 |
PRIDEi | P35192 |
PTM databases
iPTMneti | P35192 |
Interactioni
Protein-protein interaction databases
BioGridi | 35192, 278 interactors |
DIPi | DIP-4851N |
IntActi | P35192, 6 interactors |
STRINGi | 4932.YMR021C |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 264 – 279 | 1Add BLAST | 16 | |
Repeati | 322 – 337 | 2Add BLAST | 16 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 264 – 337 | 2 X 16 AA repeat of C-X-C-X(4)-C-X-C-X-X-C-X-X-HAdd BLAST | 74 |
Keywords - Domaini
RepeatPhylogenomic databases
HOGENOMi | HOG000066040 |
InParanoidi | P35192 |
OMAi | CVNCLIH |
Family and domain databases
Gene3Di | 3.90.430.10, 1 hit |
InterProi | View protein in InterPro IPR001083 Cu_fist_DNA-bd_dom IPR036395 Cu_fist_DNA-bd_dom_sf |
Pfami | View protein in Pfam PF00649 Copper-fist, 1 hit |
PRINTSi | PR00617 COPPERFIST |
SMARTi | View protein in SMART SM01090 Copper-fist, 1 hit SM00412 Cu_FIST, 1 hit |
SUPFAMi | SSF57879 SSF57879, 1 hit |
PROSITEi | View protein in PROSITE PS01119 COPPER_FIST_1, 1 hit PS50073 COPPER_FIST_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P35192-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIIFNGNKYA CASCIRGHRS STCRHSHRML IKVRTRGRPS PMAIRDAILV
60 70 80 90 100
DSTSQSTEYE NGAQIEGDCC SAMNQQPILF VRASAVRKAR MINGKLHILM
110 120 130 140 150
EEGFTAHEPK DISTFTDDGN KYITETEFLR KHSPKAPATG TISPDSTKSS
160 170 180 190 200
SSSEKKERSR LQQEPIRHFS NCCKKDKSQN PASNGKTNKA PSDDIFTPYG
210 220 230 240 250
SLESTSAFND ILQENYNSSV PGAHDSSETL TPQSTTTIAA PHSSDVASKV
260 270 280 290 300
EVLTHKGIFL STQCSCEDES CPCVNCLIHR SEEELNSYIQ QSGVPLTNIG
310 320 330 340 350
EAQITDKMMD YLDDCKCTDK ECICPPDNCT CDGCFSHSTN IIPFEKFFFY
360 370 380 390 400
GILNARLTRK TQIKFKGKLV PSKYWWDFLK LQVPLMTDAQ LELLDIHAWF
410
QKLVSNYAPH LSDATTS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 198 | P → A in CAA52645 (PubMed:8262047).Curated | 1 | |
Sequence conflicti | 380 | K → R in AAT92953 (PubMed:17322287).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X74551 Genomic DNA Translation: CAA52645.1 Z49211 Genomic DNA Translation: CAA89124.1 AY692934 Genomic DNA Translation: AAT92953.1 BK006946 Genomic DNA Translation: DAA09919.1 |
PIRi | S54023 |
RefSeqi | NP_013734.1, NM_001182517.1 |
Genome annotation databases
EnsemblFungii | YMR021C_mRNA; YMR021C; YMR021C |
GeneIDi | 855035 |
KEGGi | sce:YMR021C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X74551 Genomic DNA Translation: CAA52645.1 Z49211 Genomic DNA Translation: CAA89124.1 AY692934 Genomic DNA Translation: AAT92953.1 BK006946 Genomic DNA Translation: DAA09919.1 |
PIRi | S54023 |
RefSeqi | NP_013734.1, NM_001182517.1 |
3D structure databases
SMRi | P35192 |
ModBasei | Search... |
Protein-protein interaction databases
BioGridi | 35192, 278 interactors |
DIPi | DIP-4851N |
IntActi | P35192, 6 interactors |
STRINGi | 4932.YMR021C |
PTM databases
iPTMneti | P35192 |
Proteomic databases
MaxQBi | P35192 |
PaxDbi | P35192 |
PRIDEi | P35192 |
Genome annotation databases
EnsemblFungii | YMR021C_mRNA; YMR021C; YMR021C |
GeneIDi | 855035 |
KEGGi | sce:YMR021C |
Organism-specific databases
EuPathDBi | FungiDB:YMR021C |
SGDi | S000004623 MAC1 |
Phylogenomic databases
HOGENOMi | HOG000066040 |
InParanoidi | P35192 |
OMAi | CVNCLIH |
Enzyme and pathway databases
BioCyci | YEAST:G3O-32726-MONOMER |
Miscellaneous databases
PROi | PR:P35192 |
Family and domain databases
Gene3Di | 3.90.430.10, 1 hit |
InterProi | View protein in InterPro IPR001083 Cu_fist_DNA-bd_dom IPR036395 Cu_fist_DNA-bd_dom_sf |
Pfami | View protein in Pfam PF00649 Copper-fist, 1 hit |
PRINTSi | PR00617 COPPERFIST |
SMARTi | View protein in SMART SM01090 Copper-fist, 1 hit SM00412 Cu_FIST, 1 hit |
SUPFAMi | SSF57879 SSF57879, 1 hit |
PROSITEi | View protein in PROSITE PS01119 COPPER_FIST_1, 1 hit PS50073 COPPER_FIST_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MAC1_YEAST | |
Accessioni | P35192Primary (citable) accession number: P35192 Secondary accession number(s): D6VZJ5, E9P8Z5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | October 1, 1996 | |
Last modified: | November 13, 2019 | |
This is version 159 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XIII
Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names