UniProtKB - P35189 (TAF14_YEAST)
Transcription initiation factor TFIID subunit 14
TAF14
Functioni
Functions as a component of the DNA-binding general transcription factor complex TFIID, the RNA polymerase II associated general transcription factor complex TFIIF, and the chromatin-remodeling complex SWI/SNF (PubMed:10788514, PubMed:12138208, PubMed:12516863, PubMed:9618449).
Binding of TFIID to a promoter (with or without TATA element) is the initial step in preinitiation complex (PIC) formation (PubMed:10788514, PubMed:12138208, PubMed:12516863, PubMed:9618449).
TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription (PubMed:10788514, PubMed:12138208, PubMed:12516863, PubMed:9618449).
TFIIF is essential for the initiation of transcription by RNA polymerase II (PubMed:10788514, PubMed:12138208, PubMed:12516863, PubMed:9618449).
TFIIF functions include the recruitment of RNA polymerase II to the promoter bound DNA-TBP-TFIIB complex, decreasing the affinity of RNA polymerase II for non-specific DNA, allowing for the subsequent recruitment of TFIIE and TFIIH, and facilitating RNA polymerase II elongation (PubMed:10788514, PubMed:12138208, PubMed:12516863, PubMed:9618449, PubMed:30385749).
TAF14 acts as a chromatin reader that specifically recognizes and binds histones that are acylated (PubMed:26341557, PubMed:27089029).
Recognizes and binds histone H3 acetylated or crotonylated at 'Lys-9' (H3K9ac and H3K9cr, respectively), with some preference for crotonylated lysine (PubMed:26341557, PubMed:27089029, PubMed:30385749).
Component of the SWI/SNF complex, an ATP-dependent chromatin-remodeling complex, is required for the positive and negative regulation of gene expression of a large number of genes (PubMed:12672490).
It changes chromatin structure by altering DNA-histone contacts within a nucleosome, leading eventually to a change in nucleosome position, thus facilitating or repressing binding of gene-specific transcription factors (PubMed:12672490).
Component of the histone acetyltransferase NuA3 complex, that acetylates Lys-14 of histone H3. Recruitment of NuA3 to nucleosomes requires methylated histone H3 (PubMed:17157260).
In conjunction with the FACT complex, NuA3 may be involved in transcriptional regulation (PubMed:17157260).
Does not bind DNA (PubMed:30385749).
9 PublicationsMiscellaneous
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 104 | Acylated histone bindingCombined sources1 Publication | 1 |
GO - Molecular functioni
- histone binding Source: GO_Central
- RNA polymerase II general transcription initiation factor activity Source: SGD
GO - Biological processi
- chromatin remodeling Source: ComplexPortal
- DNA repair Source: ComplexPortal
- histone acetylation Source: SGD
- nucleosome mobilization Source: SGD
- positive regulation of double-strand break repair via homologous recombination Source: ComplexPortal
- positive regulation of transcription by RNA polymerase II Source: ComplexPortal
- regulation of transcription, DNA-templated Source: ComplexPortal
- regulation of transcription by RNA polymerase II Source: ComplexPortal
- RNA polymerase II preinitiation complex assembly Source: ComplexPortal
- transcription, DNA-templated Source: ComplexPortal
- transcription by RNA polymerase II Source: SGD
- transcription initiation from RNA polymerase II promoter Source: SGD
Keywordsi
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-SCE-674695, RNA Polymerase II Pre-transcription Events R-SCE-75955, RNA Polymerase II Transcription Elongation |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription initiation factor TFIID subunit 14Alternative name(s): Actin non-complementing mutant 1 Chromosome stability protein 10 SWI/SNF chromatin-remodeling complex subunit TAF14 SWI/SNF complex 29 kDa subunit SWI/SNF complex subunit TAF14 TBP-associated factor 14 TBP-associated factor 30 kDa Transcription factor G 30 kDa subunit Transcription initiation factor TFIIF 30 kDa subunit |
Gene namesi | Name:TAF14 Synonyms:ANC1, CST10, SWP29, TAF30, TFG3 Ordered Locus Names:YPL129W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000006050, TAF14 |
VEuPathDBi | FungiDB:YPL129W |
Subcellular locationi
Nucleus
Nucleus
- Ino80 complex Source: SGD
- mediator complex Source: SGD
- NuA3 histone acetyltransferase complex Source: SGD
- NuA3a histone acetyltransferase complex Source: SGD
- NuA3b histone acetyltransferase complex Source: SGD
- NuA4 histone acetyltransferase complex Source: GO_Central
- nucleus Source: SGD
- SWI/SNF complex Source: SGD
- Swr1 complex Source: GO_Central
- transcription factor TFIID complex Source: SGD
- transcription factor TFIIF complex Source: SGD
Other locations
- chromatin Source: ComplexPortal
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 24 | V → A: Does not affect binding to acylated histone H3. 1 Publication | 1 | |
Mutagenesisi | 80 | G → A: Abolished binding to acylated histone H3. 1 Publication | 1 | |
Mutagenesisi | 81 | W → A: Abolished binding to acetylated histone H3. 1 Publication | 1 | |
Mutagenesisi | 82 | G → A: Does not affect ability to bind crotonylated lysines, while abolishing binding to acetylated lysines. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000211241 | 1 – 244 | Transcription initiation factor TFIID subunit 14Add BLAST | 244 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 181 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources |
Keywords - PTMi
Isopeptide bond, Ubl conjugationProteomic databases
MaxQBi | P35189 |
PaxDbi | P35189 |
PRIDEi | P35189 |
PTM databases
iPTMneti | P35189 |
Interactioni
Subunit structurei
The 1.2 MDa TFIID complex is composed of TATA binding protein (TBP) and the 14 TBP-associated factors. One copy of each TAF1, TAF2, TAF3, TAF7, TAF8, TAF11, TAF13, two copies of each TAF4, TAF5, TAF6, TAF9, TAF10, TAF12, and three copies of TAF14. TFIIF is composed of three different subunits: TFG1/RAP74, TFG2/RAP30 and TAF14.
Component of the SWI/SNF global transcription activator complex. The 1.14 MDa SWI/SNF complex is composed of 11 different subunits: one copy each of SWI1, SNF2/SWI2, SNF5, SNF12/SWP73, ARP7/SWP61, ARP9/SWP59; two copies each of SWI3, SNF6, SNF11, SWP82; and three copies of TAF14/SWP29.
Component of the chromatin-remodeling INO80 complex, at least composed of ARP4, ARP5, ARP8, RVB1, RVB2, TAF14, NHP10, IES1, IES3, IES4, IES6, ACT1, IES2, IES5 and INO80.
Component of the NuA3 complex, composed of at least NTO1, SAS3, TAF14, YNG1 and EAF6.
3 PublicationsBinary interactionsi
P35189
With | #Exp. | IntAct |
---|---|---|
SAS3 [P34218] | 3 | EBI-18920,EBI-16484 |
SNF5 [P18480] | 2 | EBI-18920,EBI-17546 |
SNF6 [P18888] | 4 | EBI-18920,EBI-17550 |
YNG1 [Q08465] | 3 | EBI-18920,EBI-31890 |
GO - Molecular functioni
- histone binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 36052, 306 interactors |
ComplexPortali | CPX-1149, Transcription factor TFIIF complex CPX-1150, SWI/SNF chromatin remodelling complex CPX-1642, General transcription factor complex TFIID CPX-1810, NuA3 histone acetyltransferase complex CPX-863, INO80 chromatin remodeling complex |
DIPi | DIP-1147N |
IntActi | P35189, 53 interactors |
MINTi | P35189 |
STRINGi | 4932.YPL129W |
Miscellaneous databases
RNActi | P35189, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P35189 |
BMRBi | P35189 |
SMRi | P35189 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P35189 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 134 | YEATSPROSITE-ProRule annotationAdd BLAST | 134 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 59 – 61 | Acylated histone bindingCombined sources1 Publication | 3 | |
Regioni | 81 – 83 | Acylated histone bindingCombined sources1 Publication | 3 | |
Regioni | 133 – 169 | DisorderedSequence analysisAdd BLAST | 37 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 133 – 162 | Polar residuesSequence analysisAdd BLAST | 30 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3149, Eukaryota |
GeneTreei | ENSGT00940000176465 |
HOGENOMi | CLU_078004_0_0_1 |
InParanoidi | P35189 |
OMAi | GEFDMTI |
Family and domain databases
Gene3Di | 2.60.40.1970, 1 hit |
IDEALi | IID50207 |
InterProi | View protein in InterPro IPR027353, NET_dom IPR016665, Sas5/TAF14 IPR038704, YEAST_sf IPR005033, YEATS |
PANTHERi | PTHR23195, PTHR23195, 1 hit PTHR23195:SF2, PTHR23195:SF2, 1 hit |
Pfami | View protein in Pfam PF17035, BET, 1 hit PF03366, YEATS, 1 hit |
PIRSFi | PIRSF016551, SAS5/TFIID_14, 1 hit |
PROSITEi | View protein in PROSITE PS51037, YEATS, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MVATVKRTIR IKTQQHILPE VPPVENFPVR QWSIEIVLLD DEGKEIPATI
60 70 80 90 100
FDKVIYHLHP TFANPNRTFT DPPFRIEEQG WGGFPLDISV FLLEKAGERK
110 120 130 140 150
IPHDLNFLQE SYEVEHVIQI PLNKPLLTEE LAKSGSTEET TANTGTIGKR
160 170 180 190 200
RTTTNTTAEP KAKRAKTGSA STVKGSVDLE KLAFGLTKLN EDDLVGVVQM
210 220 230 240
VTDNKTPEMN VTNNVEEGEF IIDLYSLPEG LLKSLWDYVK KNTE
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 116 | H → Q in CAA49192 (PubMed:8423796).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U13017 Genomic DNA Translation: AAA61644.1 Z26040 Genomic DNA Translation: CAA81125.1 U43703 Genomic DNA Translation: AAB68235.1 X69394 Genomic DNA Translation: CAA49192.1 BK006949 Genomic DNA Translation: DAA11304.1 |
PIRi | S38568 |
RefSeqi | NP_015196.1, NM_001183943.1 |
Genome annotation databases
EnsemblFungii | YPL129W_mRNA; YPL129W; YPL129W |
GeneIDi | 855974 |
KEGGi | sce:YPL129W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U13017 Genomic DNA Translation: AAA61644.1 Z26040 Genomic DNA Translation: CAA81125.1 U43703 Genomic DNA Translation: AAB68235.1 X69394 Genomic DNA Translation: CAA49192.1 BK006949 Genomic DNA Translation: DAA11304.1 |
PIRi | S38568 |
RefSeqi | NP_015196.1, NM_001183943.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2L7E | NMR | - | A | 1-123 | [»] | |
3QRL | X-ray | 1.70 | A | 1-137 | [»] | |
5D7E | X-ray | 1.90 | A | 1-137 | [»] | |
5IOK | X-ray | 2.22 | A | 1-137 | [»] | |
5SVA | electron microscopy | 15.30 | n | 1-244 | [»] | |
6LQZ | NMR | - | A | 174-244 | [»] | |
6MIN | X-ray | 1.90 | A | 1-137 | [»] | |
6MIO | X-ray | 1.85 | A | 1-137 | [»] | |
6MIP | X-ray | 2.00 | A | 1-137 | [»] | |
6MIQ | X-ray | 1.75 | A | 1-137 | [»] | |
AlphaFoldDBi | P35189 | |||||
BMRBi | P35189 | |||||
SMRi | P35189 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 36052, 306 interactors |
ComplexPortali | CPX-1149, Transcription factor TFIIF complex CPX-1150, SWI/SNF chromatin remodelling complex CPX-1642, General transcription factor complex TFIID CPX-1810, NuA3 histone acetyltransferase complex CPX-863, INO80 chromatin remodeling complex |
DIPi | DIP-1147N |
IntActi | P35189, 53 interactors |
MINTi | P35189 |
STRINGi | 4932.YPL129W |
PTM databases
iPTMneti | P35189 |
Proteomic databases
MaxQBi | P35189 |
PaxDbi | P35189 |
PRIDEi | P35189 |
Protocols and materials databases
DNASUi | 855974 |
Genome annotation databases
EnsemblFungii | YPL129W_mRNA; YPL129W; YPL129W |
GeneIDi | 855974 |
KEGGi | sce:YPL129W |
Organism-specific databases
SGDi | S000006050, TAF14 |
VEuPathDBi | FungiDB:YPL129W |
Phylogenomic databases
eggNOGi | KOG3149, Eukaryota |
GeneTreei | ENSGT00940000176465 |
HOGENOMi | CLU_078004_0_0_1 |
InParanoidi | P35189 |
OMAi | GEFDMTI |
Enzyme and pathway databases
Reactomei | R-SCE-674695, RNA Polymerase II Pre-transcription Events R-SCE-75955, RNA Polymerase II Transcription Elongation |
Miscellaneous databases
EvolutionaryTracei | P35189 |
PROi | PR:P35189 |
RNActi | P35189, protein |
Family and domain databases
Gene3Di | 2.60.40.1970, 1 hit |
IDEALi | IID50207 |
InterProi | View protein in InterPro IPR027353, NET_dom IPR016665, Sas5/TAF14 IPR038704, YEAST_sf IPR005033, YEATS |
PANTHERi | PTHR23195, PTHR23195, 1 hit PTHR23195:SF2, PTHR23195:SF2, 1 hit |
Pfami | View protein in Pfam PF17035, BET, 1 hit PF03366, YEATS, 1 hit |
PIRSFi | PIRSF016551, SAS5/TFIID_14, 1 hit |
PROSITEi | View protein in PROSITE PS51037, YEATS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TAF14_YEAST | |
Accessioni | P35189Primary (citable) accession number: P35189 Secondary accession number(s): D6W3N8, Q02460 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | February 1, 1994 | |
Last modified: | May 25, 2022 | |
This is version 189 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XVI
Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families