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Protein

ATP-dependent helicase SGS1

Gene

SGS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ATP-dependent DNA helicase able to unwind duplex DNA or DNA- RNA heteroduplex (PubMed:9545297). Displacement of the DNA strand occurs in the 3' to 5' direction with respect to the single-stranded DNA flanking the duplex (PubMed:9545297). Acts as an integral component of the S-phase checkpoint response, which arrests cells due to DNA damage or blocked fork progression during DNA replication (PubMed:10640278). Can create a deleterious topological substrate that TOP3 preferentially resolves. The TOP3-SGS1 protein complex may function as a eukaryotic reverse gyrase introducing positive supercoils into extrachromosomal ribosomal DNA rings (PubMed:7969174). Together with topoisomerase II has a role in chromosomal segregation (PubMed:7736577). Maintains rDNA structure where it has a role in re-starting stalled replication forks (PubMed:12228808).5 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi714 – 721ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent 3'-5' DNA helicase activity Source: GO_Central
  • ATP-dependent DNA helicase activity Source: SGD
  • DNA binding Source: GO_Central
  • four-way junction helicase activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionHelicase, Hydrolase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32877-MONOMER
BRENDAi3.6.4.12 984
ReactomeiR-SCE-3108214 SUMOylation of DNA damage response and repair proteins

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent helicase SGS11 Publication (EC:3.6.4.121 Publication)
Alternative name(s):
Helicase TPS1
Gene namesi
Name:SGS11 Publication
Synonyms:TPS1
Ordered Locus Names:YMR190CImported
ORF Names:YM9646.02C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

SGDiS000004802 SGS1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi31Q → P in allele sgs1-34; temperature-sensitive. 1 Publication1
Mutagenesisi980T → I in allele sgs1-35; temperature-sensitive. 1 Publication1
Mutagenesisi987E → K in allele sgs1-36; temperature-sensitive. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002050571 – 1447ATP-dependent helicase SGS1Add BLAST1447

Proteomic databases

MaxQBiP35187
PaxDbiP35187
PRIDEiP35187

PTM databases

iPTMnetiP35187

Expressioni

Inductioni

Expression is regulated through the cell cycle with an accumulation in S phase.1 Publication

Interactioni

Subunit structurei

Heterodimer with TOP3 (PubMed:7969174, PubMed:15899853). Forms a complex with TOP3 and RMI1 (PubMed:15889139). Forms a ternary complex with a MLH1-MLH3 heterodimer (MutLbeta) during meiosis (PubMed:12200140). Interacts with TOP2 (PubMed:7736577).5 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi35368, 560 interactors
ComplexPortaliCPX-1071 RecQ helicase-Topo III complex
DIPiDIP-2911N
IntActiP35187, 16 interactors
MINTiP35187
STRINGi4932.YMR190C

Structurei

Secondary structure

11447
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP35187
SMRiP35187
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP35187

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini687 – 864Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST178
Domaini886 – 1035Helicase C-terminalPROSITE-ProRule annotationAdd BLAST150
Domaini1272 – 1351HRDCPROSITE-ProRule annotationAdd BLAST80

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi808 – 811DEAH box4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi631 – 639Asp/Glu-rich (highly acidic)9

Sequence similaritiesi

Belongs to the helicase family. RecQ subfamily.Curated

Phylogenomic databases

GeneTreeiENSGT00550000074520
HOGENOMiHOG000141897
InParanoidiP35187
KOiK10901
OMAiHKWLKET
OrthoDBiEOG092C0HS8

Family and domain databases

CDDicd00079 HELICc, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR002464 DNA/RNA_helicase_DEAH_CS
IPR004589 DNA_helicase_ATP-dep_RecQ
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR022758 Helicase_Sgs1
IPR010997 HRDC-like_sf
IPR002121 HRDC_dom
IPR027417 P-loop_NTPase
IPR032284 RecQ_Zn-bd
IPR018982 RQC_domain
IPR036388 WH-like_DNA-bd_sf
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
PF11408 Helicase_Sgs1, 1 hit
PF16124 RecQ_Zn_bind, 1 hit
PF09382 RQC, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SM00341 HRDC, 1 hit
SM00956 RQC, 1 hit
SUPFAMiSSF47819 SSF47819, 1 hit
SSF52540 SSF52540, 2 hits
TIGRFAMsiTIGR00614 recQ_fam, 1 hit
PROSITEiView protein in PROSITE
PS00690 DEAH_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS50967 HRDC, 1 hit

Sequencei

Sequence statusi: Complete.

P35187-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVTKPSHNLR REHKWLKETA TLQEDKDFVF QAIQKHIANK RPKTNSPPTT
60 70 80 90 100
PSKDECGPGT TNFITSIPAS GPTNTATKQH EVMQTLSNDT EWLSYTATSN
110 120 130 140 150
QYADVPMVDI PASTSVVSNP RTPNGSKTHN FNTFRPHMAS SLVENDSSRN
160 170 180 190 200
LGSRNNNKSV IDNSSIGKQL ENDIKLEVIR LQGSLIMALK EQSKLLLQKC
210 220 230 240 250
SIIESTSLSE DAKRLQLSRD IRPQLSNMSI RIDSLEKEII KAKKDGMSKD
260 270 280 290 300
QSKGRSQVSS QDDNIISSIL PSPLEYNTSS RNSNLTSTTA TTVTKALAIT
310 320 330 340 350
GAKQNITNNT GKNSNNDSNN DDLIQVLDDE DDIDCDPPVI LKEGAPHSPA
360 370 380 390 400
FPHLHMTSEE QDELTRRRNM RSREPVNYRI PDRDDPFDYV MGKSLRDDYP
410 420 430 440 450
DVEREEDELT MEAEDDAHSS YMTTRDEEKE ENELLNQSDF DFVVNDDLDP
460 470 480 490 500
TQDTDYHDNM DVSANIQESS QEGDTRSTIT LSQNKNVQVI LSSPTAQSVP
510 520 530 540 550
SNGQNQIGVE HIDLLEDDLE KDAILDDSMS FSFGRQHMPM SHSDLELIDS
560 570 580 590 600
EKENEDFEED NNNNGIEYLS DSDLERFDEE RENRTQVADI QELDNDLKII
610 620 630 640 650
TERKLTGDNE HPPPSWSPKI KREKSSVSQK DEEDDFDDDF SLSDIVSKSN
660 670 680 690 700
LSSKTNGPTY PWSDEVLYRL HEVFKLPGFR PNQLEAVNAT LQGKDVFVLM
710 720 730 740 750
PTGGGKSLCY QLPAVVKSGK THGTTIVISP LISLMQDQVE HLLNKNIKAS
760 770 780 790 800
MFSSRGTAEQ RRQTFNLFIN GLLDLVYISP EMISASEQCK RAISRLYADG
810 820 830 840 850
KLARIVVDEA HCVSNWGHDF RPDYKELKFF KREYPDIPMI ALTATASEQV
860 870 880 890 900
RMDIIHNLEL KEPVFLKQSF NRTNLYYEVN KKTKNTIFEI CDAVKSRFKN
910 920 930 940 950
QTGIIYCHSK KSCEQTSAQM QRNGIKCAYY HAGMEPDERL SVQKAWQADE
960 970 980 990 1000
IQVICATVAF GMGIDKPDVR FVYHFTVPRT LEGYYQETGR AGRDGNYSYC
1010 1020 1030 1040 1050
ITYFSFRDIR TMQTMIQKDK NLDRENKEKH LNKLQQVMAY CDNVTDCRRK
1060 1070 1080 1090 1100
LVLSYFNEDF DSKLCHKNCD NCRNSANVIN EERDVTEPAK KIVKLVESIQ
1110 1120 1130 1140 1150
NERVTIIYCQ DVFKGSRSSK IVQANHDTLE EHGIGKSMQK SEIERIFFHL
1160 1170 1180 1190 1200
ITIRVLQEYS IMNNSGFASS YVKVGPNAKK LLTGKMEIKM QFTISAPNSR
1210 1220 1230 1240 1250
PSTSSSFQAN EDNIPVIAQK STTIGGNVAA NPPRFISAKE HLRSYTYGGS
1260 1270 1280 1290 1300
TMGSSHPITL KNTSDLRSTQ ELNNLRMTYE RLRELSLNLG NRMVPPVGNF
1310 1320 1330 1340 1350
MPDSILKKMA AILPMNDSAF ATLGTVEDKY RRRFKYFKAT IADLSKKRSS
1360 1370 1380 1390 1400
EDHEKYDTIL NDEFVNRAAA SSNGIAQSTG TKSKFFGANL NEAKENEQII
1410 1420 1430 1440
NQIRQSQLPK NTTSSKSGTR SISKSSKKSA NGRRGFRNYR GHYRGRK
Length:1,447
Mass (Da):163,837
Last modified:February 1, 1994 - v1
Checksum:i0DC320B41756A3C3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22341 Genomic DNA Translation: AAB60289.1
L07870 Genomic DNA Translation: AAA35167.1
Z47815 Genomic DNA Translation: CAA87811.1
BK006946 Genomic DNA Translation: DAA10088.1
PIRiS50918
RefSeqiNP_013915.1, NM_001182696.1

Genome annotation databases

EnsemblFungiiYMR190C; YMR190C; YMR190C
GeneIDi855228
KEGGisce:YMR190C

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22341 Genomic DNA Translation: AAB60289.1
L07870 Genomic DNA Translation: AAA35167.1
Z47815 Genomic DNA Translation: CAA87811.1
BK006946 Genomic DNA Translation: DAA10088.1
PIRiS50918
RefSeqiNP_013915.1, NM_001182696.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1D8BNMR-A1271-1351[»]
ProteinModelPortaliP35187
SMRiP35187
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35368, 560 interactors
ComplexPortaliCPX-1071 RecQ helicase-Topo III complex
DIPiDIP-2911N
IntActiP35187, 16 interactors
MINTiP35187
STRINGi4932.YMR190C

PTM databases

iPTMnetiP35187

Proteomic databases

MaxQBiP35187
PaxDbiP35187
PRIDEiP35187

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR190C; YMR190C; YMR190C
GeneIDi855228
KEGGisce:YMR190C

Organism-specific databases

SGDiS000004802 SGS1

Phylogenomic databases

GeneTreeiENSGT00550000074520
HOGENOMiHOG000141897
InParanoidiP35187
KOiK10901
OMAiHKWLKET
OrthoDBiEOG092C0HS8

Enzyme and pathway databases

BioCyciYEAST:G3O-32877-MONOMER
BRENDAi3.6.4.12 984
ReactomeiR-SCE-3108214 SUMOylation of DNA damage response and repair proteins

Miscellaneous databases

EvolutionaryTraceiP35187
PROiPR:P35187

Family and domain databases

CDDicd00079 HELICc, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR002464 DNA/RNA_helicase_DEAH_CS
IPR004589 DNA_helicase_ATP-dep_RecQ
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR022758 Helicase_Sgs1
IPR010997 HRDC-like_sf
IPR002121 HRDC_dom
IPR027417 P-loop_NTPase
IPR032284 RecQ_Zn-bd
IPR018982 RQC_domain
IPR036388 WH-like_DNA-bd_sf
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
PF11408 Helicase_Sgs1, 1 hit
PF16124 RecQ_Zn_bind, 1 hit
PF09382 RQC, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SM00341 HRDC, 1 hit
SM00956 RQC, 1 hit
SUPFAMiSSF47819 SSF47819, 1 hit
SSF52540 SSF52540, 2 hits
TIGRFAMsiTIGR00614 recQ_fam, 1 hit
PROSITEiView protein in PROSITE
PS00690 DEAH_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS50967 HRDC, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSGS1_YEAST
AccessioniPrimary (citable) accession number: P35187
Secondary accession number(s): D6W014
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 7, 2018
This is version 186 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
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