UniProtKB - P35160 (RESA_BACSU)
Protein
Thiol-disulfide oxidoreductase ResA
Gene
resA
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Thiol-disulfide oxidoreductase which is required in disulfide reduction during c-type cytochrome synthesis. May accept reducing equivalents from CcdA, leading to breakage of disulfide bonds in apocytochrome c; following this reduction heme can be covalently attached. Does not play a role in sporulation.1 Publication
: cytochrome c assembly Pathwayi
This protein is involved in the pathway cytochrome c assembly, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway cytochrome c assembly and in Protein modification.
GO - Molecular functioni
- antioxidant activity Source: InterPro
- disulfide oxidoreductase activity Source: UniProtKB-UniRule
GO - Biological processi
- cytochrome complex assembly Source: UniProtKB-UniRule
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Cytochrome c-type biogenesis |
Enzyme and pathway databases
BioCyci | BSUB:BSU23150-MONOMER MetaCyc:BSU23150-MONOMER |
UniPathwayi | UPA00555 |
Names & Taxonomyi
Protein namesi | Recommended name: Thiol-disulfide oxidoreductase ResA |
Gene namesi | Name:resA Synonyms:ypxA Ordered Locus Names:BSU23150 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
- Cell membrane 1 Publication; Single-pass type II membrane protein 1 Publication Note: The thioredoxin-like motif is exposed on the outside of the membrane.
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 11 – 30 | Helical; Signal-anchor for type II membrane proteinCuratedAdd BLAST | 20 |
GO - Cellular componenti
- integral component of membrane Source: UniProtKB-KW
- plasma membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000120149 | 1 – 179 | Thiol-disulfide oxidoreductase ResAAdd BLAST | 179 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 74 ↔ 77 | Redox-active |
Keywords - PTMi
Disulfide bondProteomic databases
PRIDEi | P35160 |
Interactioni
Binary interactionsi
P35160
With | #Exp. | IntAct |
---|---|---|
cccA [P24469] | 2 | EBI-15573707,EBI-15573689 |
Protein-protein interaction databases
DIPi | DIP-61132N |
IntActi | P35160, 1 interactor |
STRINGi | 224308.BSU23150 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P35160 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P35160 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 36 – 174 | ThioredoxinAdd BLAST | 139 |
Sequence similaritiesi
Keywords - Domaini
Redox-active center, Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0526, Bacteria |
InParanoidi | P35160 |
OMAi | MIGKPFP |
PhylomeDBi | P35160 |
Family and domain databases
Gene3Di | 3.40.30.10, 1 hit |
HAMAPi | MF_01319, ResA, 1 hit |
InterProi | View protein in InterPro IPR000866, AhpC/TSA IPR023555, Thiol-dS_OxRdtase_ResA IPR036249, Thioredoxin-like_sf IPR017937, Thioredoxin_CS IPR013766, Thioredoxin_domain |
Pfami | View protein in Pfam PF00578, AhpC-TSA, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS00194, THIOREDOXIN_1, 1 hit PS51352, THIOREDOXIN_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P35160-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKKKRRLFIR TGILLVLICA LGYTIYNAVF AGKESISEGS DAPNFVLEDT
60 70 80 90 100
NGKRIELSDL KGKGVFLNFW GTWCEPCKKE FPYMANQYKH FKSQGVEIVA
110 120 130 140 150
VNVGESKIAV HNFMKSYGVN FPVVLDTDRQ VLDAYDVSPL PTTFLINPEG
160 170
KVVKVVTGTM TESMIHDYMN LIKPGETSG
Sequence cautioni
The sequence AAA67494 differs from that shown. Reason: Frameshift.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L09228 Genomic DNA Translation: AAA67494.1 Frameshift. AL009126 Genomic DNA Translation: CAB14247.2 |
PIRi | S45556 |
RefSeqi | NP_390196.2, NC_000964.3 WP_003230513.1, NZ_JNCM01000036.1 WP_009967646.1, NZ_CM000487.1 |
Genome annotation databases
EnsemblBacteriai | CAB14247; CAB14247; BSU23150 |
GeneIDi | 938958 |
KEGGi | bsu:BSU23150 |
PATRICi | fig|224308.179.peg.2522 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L09228 Genomic DNA Translation: AAA67494.1 Frameshift. AL009126 Genomic DNA Translation: CAB14247.2 |
PIRi | S45556 |
RefSeqi | NP_390196.2, NC_000964.3 WP_003230513.1, NZ_JNCM01000036.1 WP_009967646.1, NZ_CM000487.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1ST9 | X-ray | 1.50 | A/B | 37-179 | [»] | |
1SU9 | X-ray | 1.95 | A/B | 37-179 | [»] | |
2F9S | X-ray | 1.40 | A/B | 37-179 | [»] | |
2H19 | X-ray | 2.00 | A/B | 37-179 | [»] | |
2H1A | X-ray | 2.40 | A/B | 37-179 | [»] | |
2H1B | X-ray | 1.95 | A/B/C/D | 37-179 | [»] | |
2H1D | X-ray | 2.60 | A/B | 37-179 | [»] | |
2H1G | X-ray | 3.10 | A/B | 37-179 | [»] | |
3C71 | X-ray | 1.90 | A | 37-179 | [»] | |
3C73 | X-ray | 2.50 | A/B | 40-179 | [»] | |
SMRi | P35160 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-61132N |
IntActi | P35160, 1 interactor |
STRINGi | 224308.BSU23150 |
Proteomic databases
PRIDEi | P35160 |
Genome annotation databases
EnsemblBacteriai | CAB14247; CAB14247; BSU23150 |
GeneIDi | 938958 |
KEGGi | bsu:BSU23150 |
PATRICi | fig|224308.179.peg.2522 |
Phylogenomic databases
eggNOGi | COG0526, Bacteria |
InParanoidi | P35160 |
OMAi | MIGKPFP |
PhylomeDBi | P35160 |
Enzyme and pathway databases
UniPathwayi | UPA00555 |
BioCyci | BSUB:BSU23150-MONOMER MetaCyc:BSU23150-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P35160 |
Family and domain databases
Gene3Di | 3.40.30.10, 1 hit |
HAMAPi | MF_01319, ResA, 1 hit |
InterProi | View protein in InterPro IPR000866, AhpC/TSA IPR023555, Thiol-dS_OxRdtase_ResA IPR036249, Thioredoxin-like_sf IPR017937, Thioredoxin_CS IPR013766, Thioredoxin_domain |
Pfami | View protein in Pfam PF00578, AhpC-TSA, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS00194, THIOREDOXIN_1, 1 hit PS51352, THIOREDOXIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RESA_BACSU | |
Accessioni | P35160Primary (citable) accession number: P35160 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | August 29, 2003 | |
Last modified: | December 2, 2020 | |
This is version 149 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families