UniProtKB - P35129 (UBC2_CAEEL)
Protein
Ubiquitin-conjugating enzyme E2 2
Gene
let-70
Organism
Caenorhabditis elegans
Status
Functioni
Catalyzes the covalent attachment of ubiquitin to other proteins (By similarity). Mediates the selective degradation of short-lived and abnormal proteins. Plays a role in the DNA damage response (PubMed:16628214). In particular, in response to ionizing radiation, associates with the E3 ubiquitin-protein ligase brc-1-brd-1 heterodimer on chromatin to activate E3-ubiquitin ligase activity of the heterodimer, and thus its DNA damage repair mechanisms (PubMed:16628214).By similarityCurated1 Publication
Catalytic activityi
- S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation EC:2.3.2.23
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotationView all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 85 | Glycyl thioester intermediatePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ubiquitin conjugating enzyme activity Source: GO_Central
- ubiquitin protein ligase binding Source: WormBase
- ubiquitin-protein transferase activity Source: WormBase
GO - Biological processi
- protein polyubiquitination Source: GO_Central
- protein ubiquitination Source: WormBase
- ubiquitin-dependent protein catabolic process Source: WormBase
Keywordsi
Molecular function | Transferase |
Biological process | Ubl conjugation pathway |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-CEL-1234176, Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha R-CEL-201451, Signaling by BMP R-CEL-2173795, Downregulation of SMAD2/3:SMAD4 transcriptional activity R-CEL-5689896, Ovarian tumor domain proteases R-CEL-8866652, Synthesis of active ubiquitin: roles of E1 and E2 enzymes R-CEL-8866654, E3 ubiquitin ligases ubiquitinate target proteins R-CEL-8951664, Neddylation R-CEL-9033241, Peroxisomal protein import R-CEL-983168, Antigen processing: Ubiquitination & Proteasome degradation |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: Ubiquitin-conjugating enzyme E2 2 (EC:2.3.2.23)Alternative name(s): E2 ubiquitin-conjugating enzyme 2 Lethal protein 70 Ubiquitin carrier protein 2 Ubiquitin-protein ligase 2 |
Gene namesi | ORF Names:M7.1Imported |
Organismi | Caenorhabditis elegans |
Taxonomic identifieri | 6239 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditina › Rhabditomorpha › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis |
Proteomesi |
|
Organism-specific databases
WormBasei | M7.1 ; CE03482 ; WBGene00002344 ; let-70 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Other locations
- Chromosome 1 Publication
Nucleus
- nucleus Source: GO_Central
Other locations
- chromosome Source: UniProtKB-SubCell
- ubiquitin ligase complex Source: GO_Central
Keywords - Cellular componenti
Chromosome, NucleusPathology & Biotechi
Disruption phenotypei
RNAi-mediated knockdown results in reduced ubiquitination at DNA damage sites.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 75 | H → Y in s1132; hermaphrodites arrest during the early stages of larval stages. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000082515 | 1 – 147 | Ubiquitin-conjugating enzyme E2 2Add BLAST | 147 |
Proteomic databases
EPDi | P35129 |
PaxDbi | P35129 |
PeptideAtlasi | P35129 |
Expressioni
Tissue specificityi
Expressed in the nervous system.1 Publication
Developmental stagei
Expressed at all stages of development (PubMed:8441382, PubMed:8670823). Expressed in most embryonic tissues and larval tissues at the early stages (PubMed:8670823). Highly expressed in neurons, pharynx, the hyperdermis and body muscles in L1, L2, L3 and dauer larvae (PubMed:8670823). From the L4 stage of larval development and in adults, expression is mainly restricted to the nervous system, but is also expressed in the pharynx and hyperdermis (PubMed:8670823).2 Publications
Gene expression databases
Bgeei | WBGene00002344, Expressed in multi-cellular organism and 5 other tissues |
Interactioni
Subunit structurei
Interacts with the brc-1-brd-1 heterodimer following ionizing irradiation.
1 PublicationGO - Molecular functioni
- ubiquitin protein ligase binding Source: WormBase
Protein-protein interaction databases
BioGRIDi | 43106, 23 interactors |
DIPi | DIP-24306N |
IntActi | P35129, 2 interactors |
STRINGi | 6239.M7.1 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P35129 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P35129 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 147 | UBC corePROSITE-ProRule annotationAdd BLAST | 147 |
Sequence similaritiesi
Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | KOG0417, Eukaryota |
GeneTreei | ENSGT00940000155109 |
HOGENOMi | CLU_030988_13_3_1 |
InParanoidi | P35129 |
OMAi | FKPPKVH |
OrthoDBi | 1337945at2759 |
PhylomeDBi | P35129 |
Family and domain databases
CDDi | cd00195, UBCc, 1 hit |
Gene3Di | 3.10.110.10, 1 hit |
InterProi | View protein in InterPro IPR000608, UBQ-conjugat_E2 IPR023313, UBQ-conjugating_AS IPR016135, UBQ-conjugating_enzyme/RWD |
Pfami | View protein in Pfam PF00179, UQ_con, 1 hit |
SUPFAMi | SSF54495, SSF54495, 1 hit |
PROSITEi | View protein in PROSITE PS00183, UBC_1, 1 hit PS50127, UBC_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P35129-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MALKRIQKEL QDLGRDPPAQ CSAGPVGDDL FHWQATIMGP PESPYQGGVF
60 70 80 90 100
FLTIHFPTDY PFKPPKVAFT TRIYHPNINS NGSICLDILR SQWSPALTIS
110 120 130 140
KVLLSICSLL CDPNPDDPLV PEIARIYKTD RERYNQLARE WTQKYAM
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S56051 Genomic DNA Translation: AAB25489.2 Z68337 Genomic DNA Translation: CAA92745.1 |
PIRi | A48145 T23820 |
RefSeqi | NP_502065.1, NM_069664.4 |
Genome annotation databases
EnsemblMetazoai | M7.1.1; M7.1.1; WBGene00002344 |
GeneIDi | 178006 |
KEGGi | cel:CELE_M7.1 |
UCSCi | M7.1, c. elegans |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S56051 Genomic DNA Translation: AAB25489.2 Z68337 Genomic DNA Translation: CAA92745.1 |
PIRi | A48145 T23820 |
RefSeqi | NP_502065.1, NM_069664.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1Z2U | X-ray | 1.10 | A | 1-147 | [»] | |
SMRi | P35129 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 43106, 23 interactors |
DIPi | DIP-24306N |
IntActi | P35129, 2 interactors |
STRINGi | 6239.M7.1 |
Proteomic databases
EPDi | P35129 |
PaxDbi | P35129 |
PeptideAtlasi | P35129 |
Genome annotation databases
EnsemblMetazoai | M7.1.1; M7.1.1; WBGene00002344 |
GeneIDi | 178006 |
KEGGi | cel:CELE_M7.1 |
UCSCi | M7.1, c. elegans |
Organism-specific databases
CTDi | 178006 |
WormBasei | M7.1 ; CE03482 ; WBGene00002344 ; let-70 |
Phylogenomic databases
eggNOGi | KOG0417, Eukaryota |
GeneTreei | ENSGT00940000155109 |
HOGENOMi | CLU_030988_13_3_1 |
InParanoidi | P35129 |
OMAi | FKPPKVH |
OrthoDBi | 1337945at2759 |
PhylomeDBi | P35129 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Reactomei | R-CEL-1234176, Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha R-CEL-201451, Signaling by BMP R-CEL-2173795, Downregulation of SMAD2/3:SMAD4 transcriptional activity R-CEL-5689896, Ovarian tumor domain proteases R-CEL-8866652, Synthesis of active ubiquitin: roles of E1 and E2 enzymes R-CEL-8866654, E3 ubiquitin ligases ubiquitinate target proteins R-CEL-8951664, Neddylation R-CEL-9033241, Peroxisomal protein import R-CEL-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
EvolutionaryTracei | P35129 |
PROi | PR:P35129 |
Gene expression databases
Bgeei | WBGene00002344, Expressed in multi-cellular organism and 5 other tissues |
Family and domain databases
CDDi | cd00195, UBCc, 1 hit |
Gene3Di | 3.10.110.10, 1 hit |
InterProi | View protein in InterPro IPR000608, UBQ-conjugat_E2 IPR023313, UBQ-conjugating_AS IPR016135, UBQ-conjugating_enzyme/RWD |
Pfami | View protein in Pfam PF00179, UQ_con, 1 hit |
SUPFAMi | SSF54495, SSF54495, 1 hit |
PROSITEi | View protein in PROSITE PS00183, UBC_1, 1 hit PS50127, UBC_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UBC2_CAEEL | |
Accessioni | P35129Primary (citable) accession number: P35129 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | February 1, 1994 | |
Last modified: | April 7, 2021 | |
This is version 173 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Caenorhabditis annotation project |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Caenorhabditis elegans
Caenorhabditis elegans: entries, gene names and cross-references to WormBase - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families