UniProtKB - P35052 (GPC1_HUMAN)
Glypican-1
GPC1
Functioni
Cell surface proteoglycan that bears heparan sulfate. Binds, via the heparan sulfate side chains, alpha-4 (V) collagen and participates in Schwann cell myelination (By similarity).
May act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating (via the heparan sulfate side chains) with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. Required for proper skeletal muscle differentiation by sequestering FGF2 in lipid rafts preventing its binding to receptors (FGFRs) and inhibiting the FGF-mediated signaling.
By similarity2 PublicationsGO - Molecular functioni
- copper ion binding Source: UniProtKB
- fibroblast growth factor binding Source: UniProtKB
- laminin binding Source: UniProtKB
GO - Biological processi
- cell migration Source: GO_Central
- heparan sulfate proteoglycan catabolic process Source: UniProtKB
- myelin assembly Source: UniProtKB
- negative regulation of fibroblast growth factor receptor signaling pathway Source: UniProtKB
- positive regulation of skeletal muscle cell differentiation Source: UniProtKB
- regulation of protein localization to membrane Source: GO_Central
- Schwann cell differentiation Source: UniProtKB
Keywordsi
Ligand | Copper, Zinc |
Enzyme and pathway databases
PathwayCommonsi | P35052 |
Reactomei | R-HSA-1971475, A tetrasaccharide linker sequence is required for GAG synthesis R-HSA-2022928, HS-GAG biosynthesis R-HSA-2024096, HS-GAG degradation R-HSA-202733, Cell surface interactions at the vascular wall R-HSA-3560783, Defective B4GALT7 causes EDS, progeroid type R-HSA-3560801, Defective B3GAT3 causes JDSSDHD R-HSA-3656237, Defective EXT2 causes exostoses 2 R-HSA-3656253, Defective EXT1 causes exostoses 1, TRPS2 and CHDS R-HSA-376176, Signaling by ROBO receptors R-HSA-4420332, Defective B3GALT6 causes EDSP2 and SEMDJL1 R-HSA-975634, Retinoid metabolism and transport |
SignaLinki | P35052 |
SIGNORi | P35052 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:GPC1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:4449, GPC1 |
MIMi | 600395, gene |
neXtProti | NX_P35052 |
VEuPathDBi | HostDB:ENSG00000063660 |
Subcellular locationi
Endosome
Plasma membrane
Note: S-nitrosylated form recycled in endosomes. Localizes to CAV1-containing vesicles close to the cell surface. Cleavage of heparan sulfate side chains takes place mainly in late endosomes. Associates with both forms of PRNP in lipid rafts. Colocalizes with APP in perinuclear compartments and with CP in intracellular compartments. Associates with fibrillar APP amyloid-beta peptides in lipid rafts in Alzheimer disease brains.
Extracellular region or secreted
Cytosol
- cytosol Source: HPA
Endosome
- endosome Source: UniProtKB-SubCell
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
- extracellular region Source: BHF-UCL
- extracellular space Source: ProtInc
Golgi apparatus
- Golgi lumen Source: Reactome
Lysosome
- lysosomal lumen Source: Reactome
Nucleus
- nucleoplasm Source: HPA
Plasma Membrane
- anchored component of plasma membrane Source: InterPro
- intrinsic component of plasma membrane Source: UniProtKB
- plasma membrane Source: HPA
Other locations
- cell surface Source: GO_Central
- collagen-containing extracellular matrix Source: BHF-UCL
- extracellular matrix Source: UniProtKB
- membrane raft Source: UniProtKB
- synapse Source: GO_Central
Keywords - Cellular componenti
Cell membrane, Endosome, Membrane, SecretedPathology & Biotechi
Involvement in diseasei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 79 | N → Q: Protein yield reduced by half. Protein yield reduced by 90%, abolishes N-glycosylation but no effect on secondary structure; when associated with Q-116. 1 Publication | 1 | |
Mutagenesisi | 116 | N → Q: No effect on protein yield. Protein yield reduced by 90%, abolishes N-glycosylation but no effect on secondary structure; when associated with Q-79. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 2817 |
MalaCardsi | GPC1 |
NIAGADSi | ENSG00000063660 |
OpenTargetsi | ENSG00000063660 |
PharmGKBi | PA28830 |
Miscellaneous databases
Pharosi | P35052, Tbio |
Genetic variation databases
BioMutai | GPC1 |
DMDMi | 292495012 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 23 | 1 PublicationAdd BLAST | 23 | |
ChainiPRO_0000012295 | 24 – 530 | Glypican-1Add BLAST | 507 | |
ChainiPRO_0000333837 | 24 – ? | Secreted glypican-1 | ||
PropeptideiPRO_0000012296 | 531 – 558 | Removed in mature formCuratedAdd BLAST | 28 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 32 ↔ 68 | 1 Publication | ||
Disulfide bondi | 62 ↔ 256 | 1 Publication | ||
Disulfide bondi | 69 ↔ 259 | 1 Publication | ||
Glycosylationi | 79 | N-linked (GlcNAc...) asparagine2 Publications | 1 | |
Glycosylationi | 116 | N-linked (GlcNAc...) asparagine3 Publications | 1 | |
Disulfide bondi | 191 ↔ 343 | 1 Publication | ||
Disulfide bondi | 246 ↔ 279 | 1 Publication | ||
Disulfide bondi | 268 ↔ 415 | 1 Publication | ||
Disulfide bondi | 272 ↔ 401 | 1 Publication | ||
Glycosylationi | 486 | O-linked (Xyl...) (heparan sulfate) serineCurated | 1 | |
Glycosylationi | 488 | O-linked (Xyl...) (heparan sulfate) serineCurated | 1 | |
Glycosylationi | 490 | O-linked (Xyl...) (heparan sulfate) serineCurated | 1 | |
Lipidationi | 530 | GPI-anchor amidated serine1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Glycoprotein, GPI-anchor, Heparan sulfate, Lipoprotein, Proteoglycan, S-nitrosylationProteomic databases
EPDi | P35052 |
jPOSTi | P35052 |
MassIVEi | P35052 |
MaxQBi | P35052 |
PaxDbi | P35052 |
PeptideAtlasi | P35052 |
PRIDEi | P35052 |
ProteomicsDBi | 54976 [P35052-1] |
PTM databases
GlyConnecti | 1279, 2 N-Linked glycans (1 site) |
GlyGeni | P35052, 7 sites, 2 N-linked glycans (1 site), 1 O-linked glycan (1 site) |
iPTMneti | P35052 |
PhosphoSitePlusi | P35052 |
SwissPalmi | P35052 |
Expressioni
Gene expression databases
Bgeei | ENSG00000063660, Expressed in heart left ventricle and 230 other tissues |
ExpressionAtlasi | P35052, baseline and differential |
Genevisiblei | P35052, HS |
Organism-specific databases
HPAi | ENSG00000063660, Tissue enhanced (skin) |
Interactioni
Binary interactionsi
P35052
With | #Exp. | IntAct |
---|---|---|
GPR179 [Q6PRD1] | 2 | EBI-8307554,EBI-20895185 |
Gpr158 [Q8C419] from Mus musculus. | 3 | EBI-8307554,EBI-776313 |
GO - Molecular functioni
- fibroblast growth factor binding Source: UniProtKB
- laminin binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 109079, 184 interactors |
IntActi | P35052, 30 interactors |
MINTi | P35052 |
STRINGi | 9606.ENSP00000264039 |
Miscellaneous databases
RNActi | P35052, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P35052 |
SMRi | P35052 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 341 – 374 | DisorderedSequence analysisAdd BLAST | 34 | |
Regioni | 505 – 534 | DisorderedSequence analysisAdd BLAST | 30 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 353 – 367 | Basic and acidic residuesSequence analysisAdd BLAST | 15 |
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG3821, Eukaryota |
GeneTreei | ENSGT00990000203655 |
HOGENOMi | CLU_024658_2_0_1 |
InParanoidi | P35052 |
OMAi | YCAHCRG |
OrthoDBi | 611422at2759 |
PhylomeDBi | P35052 |
TreeFami | TF105317 |
Family and domain databases
InterProi | View protein in InterPro IPR001863, Glypican IPR015502, Glypican-1 IPR019803, Glypican_CS |
PANTHERi | PTHR10822, PTHR10822, 1 hit PTHR10822:SF8, PTHR10822:SF8, 1 hit |
Pfami | View protein in Pfam PF01153, Glypican, 1 hit |
PROSITEi | View protein in PROSITE PS01207, GLYPICAN, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MELRARGWWL LCAAAALVAC ARGDPASKSR SCGEVRQIYG AKGFSLSDVP
60 70 80 90 100
QAEISGEHLR ICPQGYTCCT SEMEENLANR SHAELETALR DSSRVLQAML
110 120 130 140 150
ATQLRSFDDH FQHLLNDSER TLQATFPGAF GELYTQNARA FRDLYSELRL
160 170 180 190 200
YYRGANLHLE ETLAEFWARL LERLFKQLHP QLLLPDDYLD CLGKQAEALR
210 220 230 240 250
PFGEAPRELR LRATRAFVAA RSFVQGLGVA SDVVRKVAQV PLGPECSRAV
260 270 280 290 300
MKLVYCAHCL GVPGARPCPD YCRNVLKGCL ANQADLDAEW RNLLDSMVLI
310 320 330 340 350
TDKFWGTSGV ESVIGSVHTW LAEAINALQD NRDTLTAKVI QGCGNPKVNP
360 370 380 390 400
QGPGPEEKRR RGKLAPRERP PSGTLEKLVS EAKAQLRDVQ DFWISLPGTL
410 420 430 440 450
CSEKMALSTA SDDRCWNGMA RGRYLPEVMG DGLANQINNP EVEVDITKPD
460 470 480 490 500
MTIRQQIMQL KIMTNRLRSA YNGNDVDFQD ASDDGSGSGS GDGCLDDLCS
510 520 530 540 550
RKVSRKSSSS RTPLTHALPG LSEQEGQKTS AASCPQPPTF LLPLLLFLAL
TVARPRWR
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH7C410 | H7C410_HUMAN | Glypican-1 | GPC1 | 486 | Annotation score: | ||
H7C024 | H7C024_HUMAN | Glypican-1 | GPC1 | 294 | Annotation score: | ||
H7BZL4 | H7BZL4_HUMAN | Glypican-1 | GPC1 | 281 | Annotation score: | ||
C9J4Y6 | C9J4Y6_HUMAN | Glypican-1 | GPC1 | 156 | Annotation score: | ||
H7BZE9 | H7BZE9_HUMAN | Glypican-1 | GPC1 | 162 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_036044 | 337 | A → D in a breast cancer sample; somatic mutation. 1 Publication | 1 | |
Natural variantiVAR_033977 | 500 | S → G2 PublicationsCorresponds to variant dbSNP:rs2228331Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_055225 | 1 – 72 | Missing in isoform 2. 1 PublicationAdd BLAST | 72 | |
Alternative sequenceiVSP_055226 | 295 – 359 | DSMVL…PEEKR → GEPPPARAAWNCLGECTTGG PGGRVVPSLELGPRDLIRDA LTRARSGWCCRVEGPGCLLN VLSDV in isoform 2. 1 PublicationAdd BLAST | 65 | |
Alternative sequenceiVSP_055227 | 360 – 558 | Missing in isoform 2. 1 PublicationAdd BLAST | 199 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X54232 mRNA Translation: CAA38139.1 AK095397 mRNA Translation: BAG53043.1 AK096638 mRNA Translation: BAG53345.1 AC110619 Genomic DNA Translation: AAY24160.1 CH471063 Genomic DNA Translation: EAW71180.1 CH471063 Genomic DNA Translation: EAW71183.1 BC051279 mRNA Translation: AAH51279.1 |
CCDSi | CCDS2534.1 [P35052-1] |
PIRi | A36347 |
RefSeqi | NP_002072.2, NM_002081.2 [P35052-1] |
Genome annotation databases
Ensembli | ENST00000264039.7; ENSP00000264039.2; ENSG00000063660.9 |
GeneIDi | 2817 |
KEGGi | hsa:2817 |
MANE-Selecti | ENST00000264039.7; ENSP00000264039.2; NM_002081.3; NP_002072.2 |
UCSCi | uc002vyw.5, human [P35052-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X54232 mRNA Translation: CAA38139.1 AK095397 mRNA Translation: BAG53043.1 AK096638 mRNA Translation: BAG53345.1 AC110619 Genomic DNA Translation: AAY24160.1 CH471063 Genomic DNA Translation: EAW71180.1 CH471063 Genomic DNA Translation: EAW71183.1 BC051279 mRNA Translation: AAH51279.1 |
CCDSi | CCDS2534.1 [P35052-1] |
PIRi | A36347 |
RefSeqi | NP_002072.2, NM_002081.2 [P35052-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4ACR | X-ray | 2.55 | A/B/C/D | 24-479 | [»] | |
4AD7 | X-ray | 2.94 | A/B/C/D | 24-529 | [»] | |
4BWE | X-ray | 2.46 | A/B/C/D | 24-479 | [»] | |
4YWT | X-ray | 2.38 | A/B/C/D | 24-527 | [»] | |
AlphaFoldDBi | P35052 | |||||
SMRi | P35052 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 109079, 184 interactors |
IntActi | P35052, 30 interactors |
MINTi | P35052 |
STRINGi | 9606.ENSP00000264039 |
PTM databases
GlyConnecti | 1279, 2 N-Linked glycans (1 site) |
GlyGeni | P35052, 7 sites, 2 N-linked glycans (1 site), 1 O-linked glycan (1 site) |
iPTMneti | P35052 |
PhosphoSitePlusi | P35052 |
SwissPalmi | P35052 |
Genetic variation databases
BioMutai | GPC1 |
DMDMi | 292495012 |
Proteomic databases
EPDi | P35052 |
jPOSTi | P35052 |
MassIVEi | P35052 |
MaxQBi | P35052 |
PaxDbi | P35052 |
PeptideAtlasi | P35052 |
PRIDEi | P35052 |
ProteomicsDBi | 54976 [P35052-1] |
Protocols and materials databases
Antibodypediai | 34516, 346 antibodies from 33 providers |
DNASUi | 2817 |
Genome annotation databases
Ensembli | ENST00000264039.7; ENSP00000264039.2; ENSG00000063660.9 |
GeneIDi | 2817 |
KEGGi | hsa:2817 |
MANE-Selecti | ENST00000264039.7; ENSP00000264039.2; NM_002081.3; NP_002072.2 |
UCSCi | uc002vyw.5, human [P35052-1] |
Organism-specific databases
CTDi | 2817 |
DisGeNETi | 2817 |
GeneCardsi | GPC1 |
HGNCi | HGNC:4449, GPC1 |
HPAi | ENSG00000063660, Tissue enhanced (skin) |
MalaCardsi | GPC1 |
MIMi | 600395, gene |
neXtProti | NX_P35052 |
NIAGADSi | ENSG00000063660 |
OpenTargetsi | ENSG00000063660 |
PharmGKBi | PA28830 |
VEuPathDBi | HostDB:ENSG00000063660 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3821, Eukaryota |
GeneTreei | ENSGT00990000203655 |
HOGENOMi | CLU_024658_2_0_1 |
InParanoidi | P35052 |
OMAi | YCAHCRG |
OrthoDBi | 611422at2759 |
PhylomeDBi | P35052 |
TreeFami | TF105317 |
Enzyme and pathway databases
PathwayCommonsi | P35052 |
Reactomei | R-HSA-1971475, A tetrasaccharide linker sequence is required for GAG synthesis R-HSA-2022928, HS-GAG biosynthesis R-HSA-2024096, HS-GAG degradation R-HSA-202733, Cell surface interactions at the vascular wall R-HSA-3560783, Defective B4GALT7 causes EDS, progeroid type R-HSA-3560801, Defective B3GAT3 causes JDSSDHD R-HSA-3656237, Defective EXT2 causes exostoses 2 R-HSA-3656253, Defective EXT1 causes exostoses 1, TRPS2 and CHDS R-HSA-376176, Signaling by ROBO receptors R-HSA-4420332, Defective B3GALT6 causes EDSP2 and SEMDJL1 R-HSA-975634, Retinoid metabolism and transport |
SignaLinki | P35052 |
SIGNORi | P35052 |
Miscellaneous databases
BioGRID-ORCSi | 2817, 14 hits in 1069 CRISPR screens |
ChiTaRSi | GPC1, human |
GeneWikii | Glypican_1 |
GenomeRNAii | 2817 |
Pharosi | P35052, Tbio |
PROi | PR:P35052 |
RNActi | P35052, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000063660, Expressed in heart left ventricle and 230 other tissues |
ExpressionAtlasi | P35052, baseline and differential |
Genevisiblei | P35052, HS |
Family and domain databases
InterProi | View protein in InterPro IPR001863, Glypican IPR015502, Glypican-1 IPR019803, Glypican_CS |
PANTHERi | PTHR10822, PTHR10822, 1 hit PTHR10822:SF8, PTHR10822:SF8, 1 hit |
Pfami | View protein in Pfam PF01153, Glypican, 1 hit |
PROSITEi | View protein in PROSITE PS01207, GLYPICAN, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GPC1_HUMAN | |
Accessioni | P35052Primary (citable) accession number: P35052 Secondary accession number(s): B3KTD1, Q53QM4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | March 23, 2010 | |
Last modified: | May 25, 2022 | |
This is version 183 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families