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Protein

Trypsin-3

Gene

TRYP3

Organism
Anopheles gambiae (African malaria mosquito)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Constitutive trypsin that is expressed 2 days after emergence, coinciding with host seeking behavior of the female.1 Publication

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei89Charge relay systemBy similarity1
Active sitei134Charge relay systemBy similarity1
Sitei224Required for specificityBy similarity1
Active sitei230Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processDigestion

Protein family/group databases

MEROPSiS01.130

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin-3 (EC:3.4.21.4)
Gene namesi
Name:TRYP3
ORF Names:AGAP008294
OrganismiAnopheles gambiae (African malaria mosquito)
Taxonomic identifieri7165 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraNematoceraCulicoideaCulicidaeAnophelinaeAnopheles
Proteomesi
  • UP000007062 Componentsi: Chromosome 3R, Unassembled WGS sequence

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
PropeptideiPRO_000002824723 – 48Activation peptideAdd BLAST26
ChainiPRO_000002824849 – 275Trypsin-3Add BLAST227

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi74 ↔ 90PROSITE-ProRule annotation
Disulfide bondi199 ↔ 215PROSITE-ProRule annotation
Disulfide bondi226 ↔ 250PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiP35037

Expressioni

Tissue specificityi

Expressed in the midgut. Expression levels drop a few hours after blood feeding and pick up again 28 hours later.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi7165.AGAP008294-PA

Structurei

3D structure databases

ProteinModelPortaliP35037
SMRiP35037
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini49 – 274Peptidase S1PROSITE-ProRule annotationAdd BLAST226

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
HOGENOMiHOG000251820
InParanoidiP35037
KOiK01312
OrthoDBiEOG091G0AH5

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
InterProiView protein in InterPro
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PfamiView protein in Pfam
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35037-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISNKIAILL AVLVVAVACA QARVALKHRS VQALPRFLPR PKYDVGHRIV
60 70 80 90 100
GGFEIDVSET PYQVSLQYFN SHRCGGSVLN SKWILTAAHC TVNLQPSSLA
110 120 130 140 150
VRLGSSRHAS GGTVVRVARV LEHPNYDDST IDYDFSLMEL ESELTFSDVV
160 170 180 190 200
QPVSLPDQDE AVEDGTMTIV SGWGNTQSAA ESNAILRAAN VPTVNQKECT
210 220 230 240 250
IAYSSSGGIT DRMLCAGYKR GGKDACQGDS GGPLVVDGKL VGVVSWGFGC
260 270
AMPGYPGVYA RVAVVRDWVR ENSGA
Length:275
Mass (Da):29,374
Last modified:July 1, 2008 - v5
Checksum:i93D27A26AF4C5458
GO

Sequence cautioni

The sequence EAA12261 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti42K → Q in CAA80517 (PubMed:7498434).Curated1
Sequence conflicti142S → T in CAA80517 (PubMed:7498434).Curated1
Sequence conflicti157D → E in CAA80517 (PubMed:7498434).Curated1
Sequence conflicti169I → T in CAA80517 (PubMed:7498434).Curated1
Sequence conflicti191V → I in CAA80517 (PubMed:7498434).Curated1
Sequence conflicti267D → N in CAA80517 (PubMed:7498434).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22930 Genomic DNA Translation: CAA80517.1
AAAB01008964 Genomic DNA Translation: EAA12261.3 Sequence problems.
PIRiS40007
RefSeqiXP_317171.2, XM_317171.3

Genome annotation databases

GeneIDi3291693
KEGGiaga:AgaP_AGAP008294

Similar proteinsi

Entry informationi

Entry nameiTRY3_ANOGA
AccessioniPrimary (citable) accession number: P35037
Secondary accession number(s): Q7PNF7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: July 1, 2008
Last modified: July 5, 2017
This is version 115 of the entry and version 5 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

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