UniProtKB - P35037 (TRY3_ANOGA)
Protein
Trypsin-3
Gene
TRYP3
Organism
Anopheles gambiae (African malaria mosquito)
Status
Functioni
Constitutive trypsin that is expressed 2 days after emergence, coinciding with host seeking behavior of the female.1 Publication
Catalytic activityi
- Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa. EC:3.4.21.4
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 89 | Charge relay systemBy similarity | 1 | |
Active sitei | 134 | Charge relay systemBy similarity | 1 | |
Sitei | 224 | Required for specificityBy similarity | 1 | |
Active sitei | 230 | Charge relay systemBy similarity | 1 |
GO - Molecular functioni
- serine-type endopeptidase activity Source: GO_Central
GO - Biological processi
- digestion Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Biological process | Digestion |
Protein family/group databases
MEROPSi | S01.130 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:TRYP3 ORF Names:AGAP008294 |
Organismi | Anopheles gambiae (African malaria mosquito) |
Taxonomic identifieri | 7165 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Nematocera › Culicoidea › Culicidae › Anophelinae › Anopheles › |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | VectorBase:AGAP008294 |
Subcellular locationi
Extracellular region or secreted
- Secreted 1 Publication
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 22 | Sequence analysisAdd BLAST | 22 | |
PropeptideiPRO_0000028247 | 23 – 48 | Activation peptideAdd BLAST | 26 | |
ChainiPRO_0000028248 | 49 – 275 | Trypsin-3Add BLAST | 227 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 74 ↔ 90 | PROSITE-ProRule annotation | ||
Disulfide bondi | 199 ↔ 215 | PROSITE-ProRule annotation | ||
Disulfide bondi | 226 ↔ 250 | PROSITE-ProRule annotation |
Keywords - PTMi
Disulfide bond, ZymogenProteomic databases
PaxDbi | P35037 |
Expressioni
Tissue specificityi
Expressed in the midgut. Expression levels drop a few hours after blood feeding and pick up again 28 hours later.1 Publication
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 49 – 274 | Peptidase S1PROSITE-ProRule annotationAdd BLAST | 226 |
Sequence similaritiesi
Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG3627, Eukaryota |
HOGENOMi | CLU_006842_7_0_1 |
InParanoidi | P35037 |
OrthoDBi | 1314811at2759 |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 1 hit |
InterProi | View protein in InterPro IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P35037-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MISNKIAILL AVLVVAVACA QARVALKHRS VQALPRFLPR PKYDVGHRIV
60 70 80 90 100
GGFEIDVSET PYQVSLQYFN SHRCGGSVLN SKWILTAAHC TVNLQPSSLA
110 120 130 140 150
VRLGSSRHAS GGTVVRVARV LEHPNYDDST IDYDFSLMEL ESELTFSDVV
160 170 180 190 200
QPVSLPDQDE AVEDGTMTIV SGWGNTQSAA ESNAILRAAN VPTVNQKECT
210 220 230 240 250
IAYSSSGGIT DRMLCAGYKR GGKDACQGDS GGPLVVDGKL VGVVSWGFGC
260 270
AMPGYPGVYA RVAVVRDWVR ENSGA
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A1S4GXU9 | A0A1S4GXU9_ANOGA | Trypsin 3 | 275 | Annotation score: |
Sequence cautioni
The sequence EAA12261 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 42 | K → Q in CAA80517 (PubMed:7498434).Curated | 1 | |
Sequence conflicti | 142 | S → T in CAA80517 (PubMed:7498434).Curated | 1 | |
Sequence conflicti | 157 | D → E in CAA80517 (PubMed:7498434).Curated | 1 | |
Sequence conflicti | 169 | I → T in CAA80517 (PubMed:7498434).Curated | 1 | |
Sequence conflicti | 191 | V → I in CAA80517 (PubMed:7498434).Curated | 1 | |
Sequence conflicti | 267 | D → N in CAA80517 (PubMed:7498434).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z22930 Genomic DNA Translation: CAA80517.1 AAAB01008964 Genomic DNA Translation: EAA12261.3 Sequence problems. |
PIRi | S40007 |
RefSeqi | XP_317171.2, XM_317171.3 |
Genome annotation databases
GeneIDi | 3291693 |
KEGGi | aga:AgaP_AGAP008294 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z22930 Genomic DNA Translation: CAA80517.1 AAAB01008964 Genomic DNA Translation: EAA12261.3 Sequence problems. |
PIRi | S40007 |
RefSeqi | XP_317171.2, XM_317171.3 |
3D structure databases
SMRi | P35037 |
ModBasei | Search... |
Protein family/group databases
MEROPSi | S01.130 |
Proteomic databases
PaxDbi | P35037 |
Genome annotation databases
GeneIDi | 3291693 |
KEGGi | aga:AgaP_AGAP008294 |
Organism-specific databases
CTDi | 3291693 |
VEuPathDBi | VectorBase:AGAP008294 |
Phylogenomic databases
eggNOGi | KOG3627, Eukaryota |
HOGENOMi | CLU_006842_7_0_1 |
InParanoidi | P35037 |
OrthoDBi | 1314811at2759 |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 1 hit |
InterProi | View protein in InterPro IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TRY3_ANOGA | |
Accessioni | P35037Primary (citable) accession number: P35037 Secondary accession number(s): Q7PNF7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | July 1, 2008 | |
Last modified: | February 10, 2021 | |
This is version 127 of the entry and version 5 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Peptidase families
Classification of peptidase families and list of entries