UniProtKB - P35035 (TRY1_ANOGA)
Protein
Trypsin-1
Gene
TRYP1
Organism
Anopheles gambiae (African malaria mosquito)
Status
Functioni
Major function may be to aid in digestion of the blood meal.2 Publications
Catalytic activityi
- Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa. EC:3.4.21.4
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 88 | Charge relay systemBy similarity | 1 | |
Active sitei | 133 | Charge relay systemBy similarity | 1 | |
Sitei | 223 | Required for specificityBy similarity | 1 | |
Active sitei | 229 | Charge relay systemBy similarity | 1 |
GO - Molecular functioni
- serine-type endopeptidase activity Source: GO_Central
GO - Biological processi
- digestion Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Biological process | Digestion |
Protein family/group databases
MEROPSi | S01.130 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:TRYP1 ORF Names:AGAP008296 |
Organismi | Anopheles gambiae (African malaria mosquito) |
Taxonomic identifieri | 7165 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Nematocera › Culicoidea › Culicidae › Anophelinae › Anopheles › |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | VectorBase:AGAP008296 |
Subcellular locationi
Extracellular region or secreted
- Secreted 2 Publications
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 18 | Sequence analysisAdd BLAST | 18 | |
PropeptideiPRO_0000028243 | 19 – 47 | Activation peptideAdd BLAST | 29 | |
ChainiPRO_0000028244 | 48 – 274 | Trypsin-1Add BLAST | 227 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 73 ↔ 89 | PROSITE-ProRule annotation | ||
Disulfide bondi | 198 ↔ 214 | PROSITE-ProRule annotation | ||
Disulfide bondi | 225 ↔ 249 | PROSITE-ProRule annotation |
Keywords - PTMi
Disulfide bond, ZymogenProteomic databases
PaxDbi | P35035 |
Expressioni
Tissue specificityi
Constitutively expressed at low level in the gut of adult females. Also expressed in the gut of male and female pupae.2 Publications
Inductioni
By blood meal.1 Publication
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 48 – 273 | Peptidase S1PROSITE-ProRule annotationAdd BLAST | 226 |
Sequence similaritiesi
Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG3627, Eukaryota |
HOGENOMi | CLU_006842_7_0_1 |
InParanoidi | P35035 |
OMAi | QPPRIDG |
OrthoDBi | 1314811at2759 |
PhylomeDBi | P35035 |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 1 hit |
InterProi | View protein in InterPro IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P35035-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSNKIAILLA VLVAVVACAE AQANQRHRLV RPSPSFSPRP RYAVGQRIVG
60 70 80 90 100
GFEIDVSDAP YQVSLQYNKR HNCGGSVLSS KWVLTAAHCT AGASPSSLTV
110 120 130 140 150
RLGTSRHASG GTVVRVARVV QHPKYDSSSI DFDYSLLELE DELTFSDSVQ
160 170 180 190 200
PVGLPKQDET VKDGTMTTVS GWGNTQSAAE SNAVLRAANV PTVNQKECNK
210 220 230 240 250
AYSEFGGVTD RMLCAGYQQG GKDACQGDSG GPLVADGKLV GVVSWGYGCA
260 270
QAGYPGVYSR VAVVRDWVRE NSGV
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 93 | A → R in CAA79327 (PubMed:8335004).Curated | 1 | |
Sequence conflicti | 95 | P → T in CAA79327 (PubMed:8335004).Curated | 1 | |
Sequence conflicti | 95 | P → T in CAA80512 (PubMed:7498434).Curated | 1 | |
Sequence conflicti | 101 | R → P in CAA79327 (PubMed:8335004).Curated | 1 | |
Sequence conflicti | 148 | S → A in CAA80512 (PubMed:7498434).Curated | 1 | |
Sequence conflicti | 204 | E → D in CAA79327 (PubMed:8335004).Curated | 1 | |
Sequence conflicti | 204 | E → D in CAA80512 (PubMed:7498434).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z18889 mRNA Translation: CAA79327.1 Z22930 Genomic DNA Translation: CAA80512.1 AAAB01008964 Genomic DNA Translation: EAA12590.3 |
PIRi | S35339 |
RefSeqi | XP_317170.2, XM_317170.2 |
Genome annotation databases
EnsemblMetazoai | AGAP008296-RA; AGAP008296-PA; AGAP008296 |
GeneIDi | 1277688 |
KEGGi | aga:AgaP_AGAP008296 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z18889 mRNA Translation: CAA79327.1 Z22930 Genomic DNA Translation: CAA80512.1 AAAB01008964 Genomic DNA Translation: EAA12590.3 |
PIRi | S35339 |
RefSeqi | XP_317170.2, XM_317170.2 |
3D structure databases
SMRi | P35035 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 7165.AGAP008296-PA |
Protein family/group databases
MEROPSi | S01.130 |
Proteomic databases
PaxDbi | P35035 |
Genome annotation databases
EnsemblMetazoai | AGAP008296-RA; AGAP008296-PA; AGAP008296 |
GeneIDi | 1277688 |
KEGGi | aga:AgaP_AGAP008296 |
Organism-specific databases
CTDi | 1277688 |
VEuPathDBi | VectorBase:AGAP008296 |
Phylogenomic databases
eggNOGi | KOG3627, Eukaryota |
HOGENOMi | CLU_006842_7_0_1 |
InParanoidi | P35035 |
OMAi | QPPRIDG |
OrthoDBi | 1314811at2759 |
PhylomeDBi | P35035 |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 1 hit |
InterProi | View protein in InterPro IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TRY1_ANOGA | |
Accessioni | P35035Primary (citable) accession number: P35035 Secondary accession number(s): Q7PN85 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | February 26, 2008 | |
Last modified: | February 10, 2021 | |
This is version 133 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Peptidase families
Classification of peptidase families and list of entries