UniProtKB - P35030 (TRY3_HUMAN)
Protein
Trypsin-3
Gene
PRSS3
Organism
Homo sapiens (Human)
Status
Functioni
Digestive protease that cleaves proteins preferentially after an Arg residue and has proteolytic activity toward Kunitz-type trypsin inhibitors.6 Publications
Catalytic activityi
- Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.7 Publications EC:3.4.21.4
Cofactori
Ca2+2 PublicationsNote: Binds 1 Ca2+ ion per subunit.3 Publications
Activity regulationi
Not inhibited by Kunitz-type trypsin inhibitors.2 Publications
pH dependencei
Optimum pH is 8.5.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 120 | Charge relay systemPROSITE-ProRule annotation1 Publication | 1 | |
Metal bindingi | 132 | CalciumCombined sources3 Publications | 1 | |
Metal bindingi | 134 | Calcium; via carbonyl oxygenCombined sources3 Publications | 1 | |
Metal bindingi | 137 | Calcium; via carbonyl oxygenCombined sources3 Publications | 1 | |
Metal bindingi | 139 | CalciumCombined sources3 Publications | 1 | |
Metal bindingi | 142 | CalciumCombined sources3 Publications | 1 | |
Active sitei | 164 | Charge relay systemPROSITE-ProRule annotation1 Publication | 1 | |
Sitei | 251 | Required for specificityBy similarity | 1 | |
Active sitei | 257 | Charge relay systemPROSITE-ProRule annotation1 Publication | 1 |
GO - Molecular functioni
- calcium ion binding Source: UniProtKB
- serine-type endopeptidase activity Source: UniProtKB
- serine-type peptidase activity Source: UniProtKB
GO - Biological processi
- antimicrobial humoral response Source: Reactome
- cobalamin metabolic process Source: Reactome
- digestion Source: UniProtKB
- endothelial cell migration Source: UniProtKB
- neutrophil degranulation Source: Reactome
- proteolysis Source: UniProtKB
- zymogen activation Source: UniProtKB
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Biological process | Digestion |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
PathwayCommonsi | P35030 |
Reactomei | R-HSA-1462054, Alpha-defensins R-HSA-196741, Cobalamin (Cbl, vitamin B12) transport and metabolism R-HSA-6798695, Neutrophil degranulation R-HSA-6803157, Antimicrobial peptides |
Protein family/group databases
MEROPSi | S01.174 |
Names & Taxonomyi
Protein namesi | Recommended name: Trypsin-3 (EC:3.4.21.47 Publications)Alternative name(s): Brain trypsinogen1 Publication Mesotrypsin1 Publication Mesotrypsinogen1 Publication Serine protease 3 Serine protease 4 Trypsin III Trypsin IV1 Publication |
Gene namesi | Name:PRSS3 Synonyms:PRSS4, TRY3, TRY4 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000010438.16 |
HGNCi | HGNC:9486, PRSS3 |
MIMi | 613578, gene |
neXtProti | NX_P35030 |
Subcellular locationi
Extracellular region or secreted
- Secreted 1 Publication
Extracellular region or secreted
- extracellular region Source: Reactome
- extracellular space Source: UniProtKB
Other locations
- tertiary granule lumen Source: Reactome
Keywords - Cellular componenti
SecretedPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 257 | S → A: Loss of catalytic activity. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 5646 |
OpenTargetsi | ENSG00000010438 |
PharmGKBi | PA33838 |
Miscellaneous databases
Pharosi | P35030, Tchem |
Chemistry databases
ChEMBLi | CHEMBL4551 |
DrugBanki | DB04369, 1,3,2-Dioxaborolan-2-Ol DB02308, 4-(1,3,2-Dioxaborolan-2-Yloxy)Butan-1-Aminium DB02585, 4-(Hydroxymethyl)Benzamidine DB02541, 4-Hydroxybutan-1-Aminium DB04109, [4-(1,3,2-Dioxaborolan-2-Yloxy)Methyl]Benzamidine DB03127, Benzamidine DB03129, Diamino-N-[3-(1,3,2-dioxaborolan-2-yloxy)propyl]methaniminium DB03637, Guanidine-3-propanol |
DrugCentrali | P35030 |
GuidetoPHARMACOLOGYi | 2399 |
Polymorphism and mutation databases
BioMutai | PRSS3 |
DMDMi | 209572698 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – ? | Sequence analysis | ||
PropeptideiPRO_0000028201 | ? – 80 | Activation peptide | ||
ChainiPRO_0000028202 | 81 – 304 | Trypsin-3Add BLAST | 224 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 87 ↔ 217 | Combined sources4 Publications | ||
Disulfide bondi | 105 ↔ 121 | Combined sources4 Publications | ||
Disulfide bondi | 196 ↔ 263 | Combined sources4 Publications | ||
Modified residuei | 211 | SulfotyrosineBy similarity | 1 | |
Disulfide bondi | 228 ↔ 242 | Combined sources4 Publications | ||
Disulfide bondi | 253 ↔ 277 | Combined sources4 Publications |
Keywords - PTMi
Disulfide bond, Sulfation, ZymogenProteomic databases
EPDi | P35030 |
jPOSTi | P35030 |
MassIVEi | P35030 |
MaxQBi | P35030 |
PaxDbi | P35030 |
PeptideAtlasi | P35030 |
PRIDEi | P35030 |
ProteomicsDBi | 29982 54972 [P35030-1] 54973 [P35030-2] 54974 [P35030-3] 54975 [P35030-4] |
PTM databases
iPTMneti | P35030 |
PhosphoSitePlusi | P35030 |
Expressioni
Tissue specificityi
Detected in pancreas and pancreatic fluid (at protein level) (PubMed:6698368). Expressed in pancreas and brain (PubMed:8294000). Detected in ileum (PubMed:12021776).3 Publications
Gene expression databases
Bgeei | ENSG00000010438, Expressed in pancreas and 183 other tissues |
ExpressionAtlasi | P35030, baseline and differential |
Genevisiblei | P35030, HS |
Organism-specific databases
HPAi | ENSG00000010438, Tissue enriched (pancreas) |
Interactioni
Protein-protein interaction databases
BioGRIDi | 111628, 20 interactors |
IntActi | P35030, 12 interactors |
MINTi | P35030 |
STRINGi | 9606.ENSP00000354280 |
Chemistry databases
BindingDBi | P35030 |
Miscellaneous databases
RNActi | P35030, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P35030 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P35030 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 81 – 301 | Peptidase S1PROSITE-ProRule annotationAdd BLAST | 221 |
Sequence similaritiesi
Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG3627, Eukaryota |
GeneTreei | ENSGT00990000203555 |
HOGENOMi | CLU_006842_7_1_1 |
InParanoidi | P35030 |
OMAi | HAAKIIC |
OrthoDBi | 1314811at2759 |
PhylomeDBi | P35030 |
TreeFami | TF331065 |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS |
Pfami | View protein in Pfam PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit |
s (5+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 5 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 5 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: P35030-1) [UniParc]FASTAAdd to basket
Also known as: A
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MCGPDDRCPA RWPGPGRAVK CGKGLAAARP GRVERGGAQR GGAGLELHPL
60 70 80 90 100
LGGRTWRAAR DADGCEALGT VAVPFDDDDK IVGGYTCEEN SLPYQVSLNS
110 120 130 140 150
GSHFCGGSLI SEQWVVSAAH CYKTRIQVRL GEHNIKVLEG NEQFINAAKI
160 170 180 190 200
IRHPKYNRDT LDNDIMLIKL SSPAVINARV STISLPTTPP AAGTECLISG
210 220 230 240 250
WGNTLSFGAD YPDELKCLDA PVLTQAECKA SYPGKITNSM FCVGFLEGGK
260 270 280 290 300
DSCQRDSGGP VVCNGQLQGV VSWGHGCAWK NRPGVYTKVY NYVDWIKDTI
AANS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketB1AN99 | B1AN99_HUMAN | Trypsin-3 | PRSS3 | 177 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 89 | Missing in CAA50484 (PubMed:8294000).Curated | 1 | |
Sequence conflicti | 224 – 225 | TQ → RE in CAA33527 (PubMed:2326201).Curated | 2 | |
Sequence conflicti | 253 – 254 | CQ → WK in CAA33527 (PubMed:2326201).Curated | 2 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_067459 | 174 | A → V. Corresponds to variant dbSNP:rs11547028Ensembl. | 1 | |
Natural variantiVAR_046794 | 188 | T → A7 PublicationsCorresponds to variant dbSNP:rs855581Ensembl. | 1 | |
Natural variantiVAR_059788 | 224 | T → S. Corresponds to variant dbSNP:rs1063273Ensembl. | 1 | |
Natural variantiVAR_046795 | 232 | Y → C1 PublicationCorresponds to variant dbSNP:rs1048379Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_005409 | 1 – 71 | MCGPD…ALGTV → MHMRETSGFTLKKGRSAPLV FHPPDALI in isoform 4. CuratedAdd BLAST | 71 | |
Alternative sequenceiVSP_005410 | 1 – 70 | MCGPD…EALGT → MNPFLILAFVGAA in isoform 3. 3 PublicationsAdd BLAST | 70 | |
Alternative sequenceiVSP_053779 | 1 – 70 | MCGPD…EALGT → MGPAGE in isoform 5. 1 PublicationAdd BLAST | 70 | |
Alternative sequenceiVSP_042074 | 1 – 45 | MCGPD…GGAGL → M in isoform 2. 1 PublicationAdd BLAST | 45 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X72781 mRNA Translation: CAB58178.1 X71345 mRNA Translation: CAA50484.1 X15505 mRNA Translation: CAA33527.1 D45417 mRNA Translation: BAA08257.1 AF029308 Genomic DNA Translation: AAC13322.1 AB298285 mRNA Translation: BAF80324.1 AB298286 mRNA Translation: BAF80325.1 AL139113 Genomic DNA No translation available. AL356489 Genomic DNA No translation available. AL358573 Genomic DNA No translation available. CH471071 Genomic DNA Translation: EAW58482.1 CH471071 Genomic DNA Translation: EAW58484.1 BC069476 mRNA Translation: AAH69476.1 BC069494 mRNA Translation: AAH69494.1 |
CCDSi | CCDS56570.1 [P35030-5] |
PIRi | S12764 S33496 |
RefSeqi | NP_001184026.2, NM_001197097.2 NP_001184027.1, NM_001197098.1 NP_002762.2, NM_002771.3 NP_031369.2, NM_007343.3 |
Genome annotation databases
Ensembli | ENST00000342836; ENSP00000340889; ENSG00000010438 [P35030-4] ENST00000361005; ENSP00000354280; ENSG00000010438 [P35030-1] ENST00000379405; ENSP00000368715; ENSG00000010438 [P35030-3] ENST00000429677; ENSP00000401828; ENSG00000010438 [P35030-5] |
GeneIDi | 5646 |
KEGGi | hsa:5646 |
UCSCi | uc003zti.5, human [P35030-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X72781 mRNA Translation: CAB58178.1 X71345 mRNA Translation: CAA50484.1 X15505 mRNA Translation: CAA33527.1 D45417 mRNA Translation: BAA08257.1 AF029308 Genomic DNA Translation: AAC13322.1 AB298285 mRNA Translation: BAF80324.1 AB298286 mRNA Translation: BAF80325.1 AL139113 Genomic DNA No translation available. AL356489 Genomic DNA No translation available. AL358573 Genomic DNA No translation available. CH471071 Genomic DNA Translation: EAW58482.1 CH471071 Genomic DNA Translation: EAW58484.1 BC069476 mRNA Translation: AAH69476.1 BC069494 mRNA Translation: AAH69494.1 |
CCDSi | CCDS56570.1 [P35030-5] |
PIRi | S12764 S33496 |
RefSeqi | NP_001184026.2, NM_001197097.2 NP_001184027.1, NM_001197098.1 NP_002762.2, NM_002771.3 NP_031369.2, NM_007343.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1H4W | X-ray | 1.70 | A | 81-304 | [»] | |
2R9P | X-ray | 1.40 | A/B/C/D | 81-304 | [»] | |
3L33 | X-ray | 2.48 | A/B/C/D | 81-304 | [»] | |
3L3T | X-ray | 2.38 | A/B/C/D | 81-304 | [»] | |
3P92 | X-ray | 1.60 | A | 81-304 | [»] | |
3P95 | X-ray | 1.30 | A | 81-304 | [»] | |
4DG4 | X-ray | 1.40 | A/B/D/G | 81-304 | [»] | |
4U30 | X-ray | 2.50 | A/B/C/D | 81-304 | [»] | |
4U32 | X-ray | 1.65 | A | 81-304 | [»] | |
5C67 | X-ray | 1.83 | A/B | 81-304 | [»] | |
5JBT | X-ray | 1.40 | A | 81-304 | [»] | |
5TP0 | X-ray | 1.25 | A | 81-304 | [»] | |
6BX8 | X-ray | 1.98 | A/C/E/G | 81-304 | [»] | |
6GFI | X-ray | 2.30 | A/B | 81-304 | [»] | |
SMRi | P35030 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 111628, 20 interactors |
IntActi | P35030, 12 interactors |
MINTi | P35030 |
STRINGi | 9606.ENSP00000354280 |
Chemistry databases
BindingDBi | P35030 |
ChEMBLi | CHEMBL4551 |
DrugBanki | DB04369, 1,3,2-Dioxaborolan-2-Ol DB02308, 4-(1,3,2-Dioxaborolan-2-Yloxy)Butan-1-Aminium DB02585, 4-(Hydroxymethyl)Benzamidine DB02541, 4-Hydroxybutan-1-Aminium DB04109, [4-(1,3,2-Dioxaborolan-2-Yloxy)Methyl]Benzamidine DB03127, Benzamidine DB03129, Diamino-N-[3-(1,3,2-dioxaborolan-2-yloxy)propyl]methaniminium DB03637, Guanidine-3-propanol |
DrugCentrali | P35030 |
GuidetoPHARMACOLOGYi | 2399 |
Protein family/group databases
MEROPSi | S01.174 |
PTM databases
iPTMneti | P35030 |
PhosphoSitePlusi | P35030 |
Polymorphism and mutation databases
BioMutai | PRSS3 |
DMDMi | 209572698 |
Proteomic databases
EPDi | P35030 |
jPOSTi | P35030 |
MassIVEi | P35030 |
MaxQBi | P35030 |
PaxDbi | P35030 |
PeptideAtlasi | P35030 |
PRIDEi | P35030 |
ProteomicsDBi | 29982 54972 [P35030-1] 54973 [P35030-2] 54974 [P35030-3] 54975 [P35030-4] |
Protocols and materials databases
Antibodypediai | 11047, 345 antibodies |
DNASUi | 5646 |
Genome annotation databases
Ensembli | ENST00000342836; ENSP00000340889; ENSG00000010438 [P35030-4] ENST00000361005; ENSP00000354280; ENSG00000010438 [P35030-1] ENST00000379405; ENSP00000368715; ENSG00000010438 [P35030-3] ENST00000429677; ENSP00000401828; ENSG00000010438 [P35030-5] |
GeneIDi | 5646 |
KEGGi | hsa:5646 |
UCSCi | uc003zti.5, human [P35030-1] |
Organism-specific databases
CTDi | 5646 |
DisGeNETi | 5646 |
EuPathDBi | HostDB:ENSG00000010438.16 |
GeneCardsi | PRSS3 |
HGNCi | HGNC:9486, PRSS3 |
HPAi | ENSG00000010438, Tissue enriched (pancreas) |
MIMi | 613578, gene |
neXtProti | NX_P35030 |
OpenTargetsi | ENSG00000010438 |
PharmGKBi | PA33838 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3627, Eukaryota |
GeneTreei | ENSGT00990000203555 |
HOGENOMi | CLU_006842_7_1_1 |
InParanoidi | P35030 |
OMAi | HAAKIIC |
OrthoDBi | 1314811at2759 |
PhylomeDBi | P35030 |
TreeFami | TF331065 |
Enzyme and pathway databases
PathwayCommonsi | P35030 |
Reactomei | R-HSA-1462054, Alpha-defensins R-HSA-196741, Cobalamin (Cbl, vitamin B12) transport and metabolism R-HSA-6798695, Neutrophil degranulation R-HSA-6803157, Antimicrobial peptides |
Miscellaneous databases
BioGRID-ORCSi | 5646, 4 hits in 819 CRISPR screens |
ChiTaRSi | PRSS3, human |
EvolutionaryTracei | P35030 |
GenomeRNAii | 5646 |
Pharosi | P35030, Tchem |
PROi | PR:P35030 |
RNActi | P35030, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000010438, Expressed in pancreas and 183 other tissues |
ExpressionAtlasi | P35030, baseline and differential |
Genevisiblei | P35030, HS |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS |
Pfami | View protein in Pfam PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TRY3_HUMAN | |
Accessioni | P35030Primary (citable) accession number: P35030 Secondary accession number(s): A8CED1 Q9UQV3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | October 14, 2008 | |
Last modified: | December 2, 2020 | |
This is version 202 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 9
Human chromosome 9: entries, gene names and cross-references to MIM - Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations - Peptidase families
Classification of peptidase families and list of entries