PRSS3

Functionⁱ

Digestive protease that cleaves proteins preferentially after an Arg residue and has proteolytic activity toward Kunitz-type trypsin inhibitors.

6 Publications

Catalytic activityⁱ

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
7 Publications
Manual assertion based on experiment inⁱ
- Ref.3
  "Identification and expression of the cDNA-encoding human mesotrypsin(ogen), an isoform of trypsin with inhibitor resistance."
  Nyaruhucha C.N., Kito M., Fukuoka S.I.
  J. Biol. Chem. 272:10573-10578(1997) [PubMed] [Europe PMC] [Abstract]
  Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), FUNCTION, CATALYTIC ACTIVITY, ACTIVITY REGULATION, VARIANT ALA-188.
- Ref.10
  "Mesotrypsin: a new inhibitor-resistant protease from a zymogen in human pancreatic tissue and fluid."
  Rinderknecht H., Renner I.G., Abramson S.B., Carmack C.
  Gastroenterology 86:681-692(1984) [PubMed] [Europe PMC] [Abstract]
  Cited for: CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
- Ref.11
  "Human mesotrypsin is a unique digestive protease specialized for the degradation of trypsin inhibitors."
  Szmola R., Kukor Z., Sahin-Toth M.
  J. Biol. Chem. 278:48580-48589(2003) [PubMed] [Europe PMC] [Abstract]
  Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR.
- Ref.12
  "Crystal structure reveals basis for the inhibitor resistance of human brain trypsin."
  Katona G., Berglund G.I., Hajdu J., Graf L., Szilagyi L.
  J. Mol. Biol. 315:1209-1218(2002) [PubMed] [Europe PMC] [Abstract]
  Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 81-304 (ISOFORM A) IN COMPLEX WITH CALCIUM IONS, COFACTOR, CATALYTIC ACTIVITY, DISULFIDE BONDS.
- Ref.13
  "Structural basis for accelerated cleavage of bovine pancreatic trypsin inhibitor (BPTI) by human mesotrypsin."
  Salameh M.A., Soares A.S., Hockla A., Radisky E.S.
  J. Biol. Chem. 283:4115-4123(2008) [PubMed] [Europe PMC] [Abstract]
  Cited for: X-RAY CRYSTALLOGRAPHY (1.40 ANGSTROMS) OF 81-304 OF MUTANT ALA-257, FUNCTION, CATALYTIC ACTIVITY, ACTIVITY REGULATION, DISULFIDE BONDS.
- Ref.14
  "Sequence and conformational specificity in substrate recognition: several human Kunitz protease inhibitor domains are specific substrates of mesotrypsin."
  Pendlebury D., Wang R., Henin R.D., Hockla A., Soares A.S., Madden B.J., Kazanov M.D., Radisky E.S.
  J. Biol. Chem. 289:32783-32797(2014) [PubMed] [Europe PMC] [Abstract]
  Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF 81-304 IN COMPLEX WITH CALCIUM, CATALYTIC ACTIVITY, FUNCTION, COFACTOR, DISULFIDE BONDS.
- Ref.15
  "An Acrobatic Substrate Metamorphosis Reveals a Requirement for Substrate Conformational Dynamics in Trypsin Proteolysis."
  Kayode O., Wang R., Pendlebury D.F., Cohen I., Henin R.D., Hockla A., Soares A.S., Papo N., Caulfield T.R., Radisky E.S.
  J. Biol. Chem. 291:26304-26319(2016) [PubMed] [Europe PMC] [Abstract]
  Cited for: X-RAY CRYSTALLOGRAPHY (1.40 ANGSTROMS) OF 81-304 OF MUTANT ALA-257 IN COMPLEX WITH CALCIUM, FUNCTION, CATALYTIC ACTIVITY, COFACTOR, DISULFIDE BONDS, ACTIVE SITE, MUTAGENESIS OF SER-257.
EC:3.4.21.4

Cofactorⁱ

Ca²⁺2 PublicationsNote: Binds 1 Ca²⁺ ion per subunit.3 Publications

Activity regulationⁱ

Not inhibited by Kunitz-type trypsin inhibitors.2 Publications

pH dependenceⁱ

Optimum pH is 8.5.1 Publication

Sites

Feature key	Position(s)	DescriptionActions	Length
Active siteⁱ	120	Charge relay systemPROSITE-ProRule annotation1 Publication	1
Metal bindingⁱ	132	CalciumCombined sources3 Publications	1
Metal bindingⁱ	134	Calcium; via carbonyl oxygenCombined sources3 Publications	1
Metal bindingⁱ	137	Calcium; via carbonyl oxygenCombined sources3 Publications	1
Metal bindingⁱ	139	CalciumCombined sources3 Publications	1
Metal bindingⁱ	142	CalciumCombined sources3 Publications	1
Active siteⁱ	164	Charge relay systemPROSITE-ProRule annotation1 Publication	1
Siteⁱ	251	Required for specificityBy similarity	1
Active siteⁱ	257	Charge relay systemPROSITE-ProRule annotation1 Publication	1

GO - Molecular functionⁱ

Complete GO annotation on QuickGO ...

GO - Biological processⁱ

Complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Hydrolase, Protease, Serine protease
Biological process	Digestion
Ligand	Calcium, Metal-binding

Enzyme and pathway databases

PathwayCommonsⁱ	P35030
Reactomeⁱ	R-HSA-1462054, Alpha-defensins R-HSA-196741, Cobalamin (Cbl, vitamin B12) transport and metabolism R-HSA-6798695, Neutrophil degranulation R-HSA-6803157, Antimicrobial peptides
SIGNORⁱ	P35030

Protein family/group databases

MEROPS protease database More...MEROPSⁱ	S01.174

MEROPSⁱ

S01.174

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Trypsin-3 (EC:3.4.21.47 Publications) Alternative name(s): Brain trypsinogen1 Publication Mesotrypsin1 Publication Mesotrypsinogen1 Publication Serine protease 3 Serine protease 4 Trypsin III Trypsin IV1 Publication
Gene namesⁱ	Name:PRSS3 Synonyms:PRSS4, TRY3, TRY4
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 9

Organism-specific databases

Human Gene Nomenclature Database More...HGNCⁱ	HGNC:9486, PRSS3
MIMⁱ	613578, gene
neXtProtⁱ	NX_P35030
VEuPathDBⁱ	HostDB:ENSG00000010438

Keywords - Cellular componentⁱ

Secreted

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	257	S → A: Loss of catalytic activity. 1 Publication		1

Organism-specific databases

DisGeNET More...DisGeNETⁱ	5646
Open Targets More...OpenTargetsⁱ	ENSG00000010438
PharmGKBⁱ	PA33838

Miscellaneous databases

Pharosⁱ	P35030, Tchem

Pharosⁱ

P35030, Tchem

Chemistry databases

ChEMBLⁱ	CHEMBL4551
Drug and drug target database More...DrugBankⁱ	DB04369, 1,3,2-Dioxaborolan-2-Ol DB02308, 4-(1,3,2-Dioxaborolan-2-Yloxy)Butan-1-Aminium DB02585, 4-(Hydroxymethyl)Benzamidine DB02541, 4-Hydroxybutan-1-Aminium DB04109, [4-(1,3,2-Dioxaborolan-2-Yloxy)Methyl]Benzamidine DB03127, Benzamidine DB03129, Diamino-N-[3-(1,3,2-dioxaborolan-2-yloxy)propyl]methaniminium DB03637, Guanidine-3-propanol
DrugCentral More...DrugCentralⁱ	P35030
IUPHAR/BPS Guide to PHARMACOLOGY More...GuidetoPHARMACOLOGYⁱ	2399

Genetic variation databases

BioMutaⁱ	PRSS3
DMDMⁱ	209572698

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Length
Signal peptideⁱ	1 – ?	Sequence analysis
PropeptideⁱPRO_0000028201	? – 80	Activation peptide
ChainⁱPRO_0000028202	81 – 304	Trypsin-3Add BLAST	224

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Disulfide bondⁱ	87 ↔ 217	Combined sources4 Publications
Disulfide bondⁱ	105 ↔ 121	Combined sources4 Publications
Disulfide bondⁱ	196 ↔ 263	Combined sources4 Publications
Modified residueⁱ	211	SulfotyrosineBy similarity	1
Disulfide bondⁱ	228 ↔ 242	Combined sources4 Publications
Disulfide bondⁱ	253 ↔ 277	Combined sources4 Publications

Keywords - PTMⁱ

Disulfide bond, Sulfation, Zymogen

Proteomic databases

EPDⁱ	P35030
jPOSTⁱ	P35030
MassIVEⁱ	P35030
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P35030
PaxDbⁱ	P35030
PeptideAtlas More...PeptideAtlasⁱ	P35030
PRIDEⁱ	P35030
ProteomicsDBⁱ	29982 54972 [P35030-1] 54973 [P35030-2] 54974 [P35030-3] 54975 [P35030-4]

PTM databases

iPTMnetⁱ	P35030
PhosphoSitePlusⁱ	P35030

Expressionⁱ

Tissue specificityⁱ

Detected in pancreas and pancreatic fluid (at protein level) (PubMed:6698368). Expressed in pancreas and brain (PubMed:8294000). Detected in ileum (PubMed:12021776).3 Publications

Gene expression databases

Bgeeⁱ	ENSG00000010438, Expressed in pancreas and 184 other tissues
ExpressionAtlasⁱ	P35030, baseline and differential
Genevisibleⁱ	P35030, HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	ENSG00000010438, Tissue enriched (pancreas)

HPAⁱ

ENSG00000010438, Tissue enriched (pancreas)

Interactionⁱ

Protein-protein interaction databases

BioGRIDⁱ	111628, 43 interactors
IntActⁱ	P35030, 23 interactors
Molecular INTeraction database More...MINTⁱ	P35030
STRINGⁱ	9606.ENSP00000354280

Chemistry databases

BindingDBⁱ

P35030

Miscellaneous databases

RNActⁱ	P35030, protein

RNActⁱ

P35030, protein

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	89 – 91	Combined sources	3
Beta strandⁱ	95 – 111	Combined sources	17
Beta strandⁱ	114 – 117	Combined sources	4
Helixⁱ	119 – 121	Combined sources	3
Beta strandⁱ	127 – 131	Combined sources	5
Beta strandⁱ	133 – 137	Combined sources	5
Beta strandⁱ	143 – 152	Combined sources	10
Turnⁱ	158 – 160	Combined sources	3
Beta strandⁱ	166 – 172	Combined sources	7
Beta strandⁱ	177 – 180	Combined sources	4
Beta strandⁱ	195 – 201	Combined sources	7
Beta strandⁱ	206 – 208	Combined sources	3
Beta strandⁱ	216 – 222	Combined sources	7
Helixⁱ	225 – 231	Combined sources	7
Turnⁱ	233 – 235	Combined sources	3
Beta strandⁱ	240 – 244	Combined sources	5
Beta strandⁱ	246 – 249	Combined sources	4
Turnⁱ	254 – 258	Combined sources	5
Beta strandⁱ	260 – 263	Combined sources	4
Beta strandⁱ	266 – 273	Combined sources	8
Beta strandⁱ	275 – 278	Combined sources	4
Beta strandⁱ	284 – 288	Combined sources	5
Helixⁱ	289 – 292	Combined sources	4
Helixⁱ	293 – 302	Combined sources	10

Show more details

3D structure databases

SMRⁱ	P35030
ModBaseⁱ	Search...
PDBe-KBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	P35030

EvolutionaryTraceⁱ

P35030

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	81 – 301	Peptidase S1PROSITE-ProRule annotationAdd BLAST		221

Sequence similaritiesⁱ

Belongs to the peptidase S1 family.PROSITE-ProRule annotation

Keywords - Domainⁱ

Signal

Phylogenomic databases

eggNOGⁱ	KOG3627, Eukaryota
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00990000203555
HOGENOMⁱ	CLU_006842_7_1_1
InParanoidⁱ	P35030
OMAⁱ	HAAKIIC
Database of Orthologous Groups More...OrthoDBⁱ	1314811at2759
PhylomeDBⁱ	P35030
TreeFamⁱ	TF331065

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00190, Tryp_SPc, 1 hit
Gene3Dⁱ	2.40.10.10, 2 hits
InterProⁱ	View protein in InterPro IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00089, Trypsin, 1 hit
PRINTSⁱ	PR00722, CHYMOTRYPSIN
SMARTⁱ	View protein in SMART SM00020, Tryp_SPc, 1 hit
SUPFAMⁱ	SSF50494, SSF50494, 1 hit
PROSITEⁱ	View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit

Sequences (5+)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry describes 5 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 5 described isoforms and 2 potential isoforms that are computationally mapped.Show all Align All

Isoform 1 (identifier: P35030-1) [UniParc]FASTA Add to basket

Also known as: A

This isoform has been chosen as the canonicalⁱ sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MCGPDDRCPA RWPGPGRAVK CGKGLAAARP GRVERGGAQR GGAGLELHPL 
        60         70         80         90        100
LGGRTWRAAR DADGCEALGT VAVPFDDDDK IVGGYTCEEN SLPYQVSLNS 
       110        120        130        140        150
GSHFCGGSLI SEQWVVSAAH CYKTRIQVRL GEHNIKVLEG NEQFINAAKI 
       160        170        180        190        200
IRHPKYNRDT LDNDIMLIKL SSPAVINARV STISLPTTPP AAGTECLISG 
       210        220        230        240        250
WGNTLSFGAD YPDELKCLDA PVLTQAECKA SYPGKITNSM FCVGFLEGGK 
       260        270        280        290        300
DSCQRDSGGP VVCNGQLQGV VSWGHGCAWK NRPGVYTKVY NYVDWIKDTI 

AANS

Length:304

Mass (Da):32,529

Last modified:October 14, 2008 - v2

Checksum:ⁱ4C4303C310B7BFFC

GO

Isoform 2 (identifier: P35030-2) [UniParc]FASTA Add to basket

Also known as: B

The sequence of this isoform differs from the canonical sequence as follows:
1-45: MCGPDDRCPARWPGPGRAVKCGKGLAAARPGRVERGGAQRGGAGL → M

« Hide

        10         20         30         40         50
MELHPLLGGR TWRAARDADG CEALGTVAVP FDDDDKIVGG YTCEENSLPY 
        60         70         80         90        100
QVSLNSGSHF CGGSLISEQW VVSAAHCYKT RIQVRLGEHN IKVLEGNEQF 
       110        120        130        140        150
INAAKIIRHP KYNRDTLDND IMLIKLSSPA VINARVSTIS LPTTPPAAGT 
       160        170        180        190        200
ECLISGWGNT LSFGADYPDE LKCLDAPVLT QAECKASYPG KITNSMFCVG 
       210        220        230        240        250
FLEGGKDSCQ RDSGGPVVCN GQLQGVVSWG HGCAWKNRPG VYTKVYNYVD 
       260
WIKDTIAANS

Show »

Length:260

Mass (Da):28,161

Checksum:ⁱCD8AA6E8072BCE56

GO

Isoform 3 (identifier: P35030-3) [UniParc]FASTA Add to basket

Also known as: C

The sequence of this isoform differs from the canonical sequence as follows:
1-70: MCGPDDRCPA...DADGCEALGT → MNPFLILAFVGAA

« Hide

        10         20         30         40         50
MNPFLILAFV GAAVAVPFDD DDKIVGGYTC EENSLPYQVS LNSGSHFCGG 
        60         70         80         90        100
SLISEQWVVS AAHCYKTRIQ VRLGEHNIKV LEGNEQFINA AKIIRHPKYN 
       110        120        130        140        150
RDTLDNDIML IKLSSPAVIN ARVSTISLPT TPPAAGTECL ISGWGNTLSF 
       160        170        180        190        200
GADYPDELKC LDAPVLTQAE CKASYPGKIT NSMFCVGFLE GGKDSCQRDS 
       210        220        230        240 
GGPVVCNGQL QGVVSWGHGC AWKNRPGVYT KVYNYVDWIK DTIAANS

Show »

Length:247

Mass (Da):26,727

Checksum:ⁱ01563656780A6607

GO

Isoform 4 (identifier: P35030-4) [UniParc]FASTA Add to basket

Also known as: D

The sequence of this isoform differs from the canonical sequence as follows:
1-71: MCGPDDRCPA...ADGCEALGTV → MHMRETSGFTLKKGRSAPLVFHPPDALI

« Hide

        10         20         30         40         50
MHMRETSGFT LKKGRSAPLV FHPPDALIAV PFDDDDKIVG GYTCEENSLP 
        60         70         80         90        100
YQVSLNSGSH FCGGSLISEQ WVVSAAHCYK TRIQVRLGEH NIKVLEGNEQ 
       110        120        130        140        150
FINAAKIIRH PKYNRDTLDN DIMLIKLSSP AVINARVSTI SLPTTPPAAG 
       160        170        180        190        200
TECLISGWGN TLSFGADYPD ELKCLDAPVL TQAECKASYP GKITNSMFCV 
       210        220        230        240        250
GFLEGGKDSC QRDSGGPVVC NGQLQGVVSW GHGCAWKNRP GVYTKVYNYV 
       260 
DWIKDTIAAN S

Show »

Length:261

Mass (Da):28,402

Checksum:ⁱ5908225118767A4A

GO

Isoform 5 (identifier: P35030-5) [UniParc]FASTA Add to basket

Also known as: E

The sequence of this isoform differs from the canonical sequence as follows:
1-70: MCGPDDRCPA...DADGCEALGT → MGPAGE

« Hide

        10         20         30         40         50
MGPAGEVAVP FDDDDKIVGG YTCEENSLPY QVSLNSGSHF CGGSLISEQW 
        60         70         80         90        100
VVSAAHCYKT RIQVRLGEHN IKVLEGNEQF INAAKIIRHP KYNRDTLDND 
       110        120        130        140        150
IMLIKLSSPA VINARVSTIS LPTTPPAAGT ECLISGWGNT LSFGADYPDE 
       160        170        180        190        200
LKCLDAPVLT QAECKASYPG KITNSMFCVG FLEGGKDSCQ RDSGGPVVCN 
       210        220        230        240
GQLQGVVSWG HGCAWKNRPG VYTKVYNYVD WIKDTIAANS

Show »

Length:240

Mass (Da):25,924

Checksum:ⁱE8DF46CFF536DB74

GO

Computationally mapped potential isoform sequencesⁱ

There are 2 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

A0A7P0MNE9	A0A7P0MNE9_HUMAN	Trypsin-3 Trypsin-3	PRSS3	259	Annotation score:
A0A7P0MP65	A0A7P0MP65_HUMAN	Trypsin-3 Trypsin-3	PRSS3	139	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	89	Missing in CAA50484 (PubMed:8294000).Curated	1
Sequence conflictⁱ	224 – 225	TQ → RE in CAA33527 (PubMed:2326201).Curated	2
Sequence conflictⁱ	253 – 254	CQ → WK in CAA33527 (PubMed:2326201).Curated	2

Natural variant

Feature key	Position(s)	DescriptionActions	Length
Natural variantⁱVAR_067459	174	A → V. Corresponds to variant dbSNP:rs11547028Ensembl.	1
Natural variantⁱVAR_046794	188	T → A7 PublicationsCorresponds to variant dbSNP:rs855581Ensembl.	1
Natural variantⁱVAR_059788	224	T → S. Corresponds to variant dbSNP:rs1063273Ensembl.	1
Natural variantⁱVAR_046795	232	Y → C1 PublicationCorresponds to variant dbSNP:rs1048379Ensembl.	1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Length
Alternative sequenceⁱVSP_005409	1 – 71	MCGPD…ALGTV → MHMRETSGFTLKKGRSAPLV FHPPDALI in isoform 4. CuratedAdd BLAST	71
Alternative sequenceⁱVSP_005410	1 – 70	MCGPD…EALGT → MNPFLILAFVGAA in isoform 3. 3 PublicationsAdd BLAST	70
Alternative sequenceⁱVSP_053779	1 – 70	MCGPD…EALGT → MGPAGE in isoform 5. 1 PublicationAdd BLAST	70
Alternative sequenceⁱVSP_042074	1 – 45	MCGPD…GGAGL → M in isoform 2. 1 PublicationAdd BLAST	45

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X72781 mRNA Translation: CAB58178.1 X71345 mRNA Translation: CAA50484.1 X15505 mRNA Translation: CAA33527.1 D45417 mRNA Translation: BAA08257.1 AF029308 Genomic DNA Translation: AAC13322.1 AB298285 mRNA Translation: BAF80324.1 AB298286 mRNA Translation: BAF80325.1 AL139113 Genomic DNA No translation available. AL356489 Genomic DNA No translation available. AL358573 Genomic DNA No translation available. CH471071 Genomic DNA Translation: EAW58482.1 CH471071 Genomic DNA Translation: EAW58484.1 BC069476 mRNA Translation: AAH69476.1 BC069494 mRNA Translation: AAH69494.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS56570.1 [P35030-5] CCDS56571.1 [P35030-4] CCDS6545.1 [P35030-3]
PIRⁱ	S12764 S33496
NCBI Reference Sequences More...RefSeqⁱ	NP_001184026.2, NM_001197097.2 [P35030-4] NP_001184027.1, NM_001197098.1 NP_002762.2, NM_002771.3 [P35030-3] NP_031369.2, NM_007343.3

Genome annotation databases

Ensemblⁱ	ENST00000379405; ENSP00000368715; ENSG00000010438 [P35030-3] ENST00000429677; ENSP00000401828; ENSG00000010438 [P35030-5]
GeneIDⁱ	5646
KEGGⁱ	hsa:5646
UCSC genome browser More...UCSCⁱ	uc003zti.5, human [P35030-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P35030-4	Trypsin-3		HUMAN		259	UniRef100_A0A7P0MNE9

Protein	Similar proteins		Species	Score	Length	Source
P35030	Serine protease 3		PONAB		303	UniRef90_P35030
Serine protease 3		GORGO		302
Serine protease 3		PANTR		291
Isoform 2 of Trypsin-3		HUMAN		260
PRSS3 protein (Fragment)	)	HUMAN		251
+5
P35030-4	Isoform 5 of Trypsin-3		HUMAN		240	UniRef90_P35030-5
Serine protease 3		GORGO		261
Serine protease 3		PANPA		261
Serine protease 3		PANTR		260
Protease serine 4 isoform B		HUMAN		261
+14

Protein	Similar proteins		Species	Score	Length	Source
P35030	Serine protease 3		PONAB		303	UniRef50_P35030
Serine protease 3		GORGO		302
Serine protease 3		PANTR		291
Isoform 2 of Trypsin-3		HUMAN		260
Peptidase S1 domain-containing protein		MACFA		252
+82
P35030-4	Isoform 5 of Trypsin-3		HUMAN		240	UniRef50_P35030-5
Trypsin	)	PIG		231
Trypsin		XENLA		243
Anionic trypsin-2		MOUSE		246
Trypsin I-P38		CHICK		248
+930

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X72781 mRNA Translation: CAB58178.1 X71345 mRNA Translation: CAA50484.1 X15505 mRNA Translation: CAA33527.1 D45417 mRNA Translation: BAA08257.1 AF029308 Genomic DNA Translation: AAC13322.1 AB298285 mRNA Translation: BAF80324.1 AB298286 mRNA Translation: BAF80325.1 AL139113 Genomic DNA No translation available. AL356489 Genomic DNA No translation available. AL358573 Genomic DNA No translation available. CH471071 Genomic DNA Translation: EAW58482.1 CH471071 Genomic DNA Translation: EAW58484.1 BC069476 mRNA Translation: AAH69476.1 BC069494 mRNA Translation: AAH69494.1
CCDSⁱ	CCDS56570.1 [P35030-5] CCDS56571.1 [P35030-4] CCDS6545.1 [P35030-3]
PIRⁱ	S12764 S33496
RefSeqⁱ	NP_001184026.2, NM_001197097.2 [P35030-4] NP_001184027.1, NM_001197098.1 NP_002762.2, NM_002771.3 [P35030-3] NP_031369.2, NM_007343.3

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1H4W	X-ray	1.70	A	81-304	[»]
	2R9P	X-ray	1.40	A/B/C/D	81-304	[»]
	3L33	X-ray	2.48	A/B/C/D	81-304	[»]
	3L3T	X-ray	2.38	A/B/C/D	81-304	[»]
	3P92	X-ray	1.60	A	81-304	[»]
	3P95	X-ray	1.30	A	81-304	[»]
	4DG4	X-ray	1.40	A/B/D/G	81-304	[»]
	4U30	X-ray	2.50	A/B/C/D	81-304	[»]
	4U32	X-ray	1.65	A	81-304	[»]
	5C67	X-ray	1.83	A/B	81-304	[»]
	5JBT	X-ray	1.40	A	81-304	[»]
	5TP0	X-ray	1.25	A	81-304	[»]
	6BX8	X-ray	1.98	A/C/E/G	81-304	[»]
	6GFI	X-ray	2.30	A/B	81-304	[»]
SMRⁱ	P35030
ModBaseⁱ	Search...
PDBe-KBⁱ	Search...

Protein-protein interaction databases

BioGRIDⁱ	111628, 43 interactors
IntActⁱ	P35030, 23 interactors
MINTⁱ	P35030
STRINGⁱ	9606.ENSP00000354280

Chemistry databases

BindingDBⁱ	P35030
ChEMBLⁱ	CHEMBL4551
DrugBankⁱ	DB04369, 1,3,2-Dioxaborolan-2-Ol DB02308, 4-(1,3,2-Dioxaborolan-2-Yloxy)Butan-1-Aminium DB02585, 4-(Hydroxymethyl)Benzamidine DB02541, 4-Hydroxybutan-1-Aminium DB04109, [4-(1,3,2-Dioxaborolan-2-Yloxy)Methyl]Benzamidine DB03127, Benzamidine DB03129, Diamino-N-[3-(1,3,2-dioxaborolan-2-yloxy)propyl]methaniminium DB03637, Guanidine-3-propanol
DrugCentralⁱ	P35030
GuidetoPHARMACOLOGYⁱ	2399

Protein family/group databases

MEROPSⁱ	S01.174

MEROPSⁱ

S01.174

PTM databases

iPTMnetⁱ	P35030
PhosphoSitePlusⁱ	P35030

Genetic variation databases

BioMutaⁱ	PRSS3
DMDMⁱ	209572698

Proteomic databases

EPDⁱ	P35030
jPOSTⁱ	P35030
MassIVEⁱ	P35030
MaxQBⁱ	P35030
PaxDbⁱ	P35030
PeptideAtlasⁱ	P35030
PRIDEⁱ	P35030
ProteomicsDBⁱ	29982 54972 [P35030-1] 54973 [P35030-2] 54974 [P35030-3] 54975 [P35030-4]

Protocols and materials databases

Antibodypediaⁱ	11047, 354 antibodies
The DNASU plasmid repository More...DNASUⁱ	5646

Genome annotation databases

Ensemblⁱ	ENST00000379405; ENSP00000368715; ENSG00000010438 [P35030-3] ENST00000429677; ENSP00000401828; ENSG00000010438 [P35030-5]
GeneIDⁱ	5646
KEGGⁱ	hsa:5646
UCSCⁱ	uc003zti.5, human [P35030-1]

Organism-specific databases

CTDⁱ	5646
DisGeNETⁱ	5646
GeneCardsⁱ	PRSS3
HGNCⁱ	HGNC:9486, PRSS3
HPAⁱ	ENSG00000010438, Tissue enriched (pancreas)
MIMⁱ	613578, gene
neXtProtⁱ	NX_P35030
OpenTargetsⁱ	ENSG00000010438
PharmGKBⁱ	PA33838
VEuPathDBⁱ	HostDB:ENSG00000010438
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG3627, Eukaryota
GeneTreeⁱ	ENSGT00990000203555
HOGENOMⁱ	CLU_006842_7_1_1
InParanoidⁱ	P35030
OMAⁱ	HAAKIIC
OrthoDBⁱ	1314811at2759
PhylomeDBⁱ	P35030
TreeFamⁱ	TF331065

Enzyme and pathway databases

PathwayCommonsⁱ	P35030
Reactomeⁱ	R-HSA-1462054, Alpha-defensins R-HSA-196741, Cobalamin (Cbl, vitamin B12) transport and metabolism R-HSA-6798695, Neutrophil degranulation R-HSA-6803157, Antimicrobial peptides
SIGNORⁱ	P35030

Miscellaneous databases

BioGRID-ORCSⁱ	5646, 7 hits in 978 CRISPR screens
ChiTaRSⁱ	PRSS3, human
EvolutionaryTraceⁱ	P35030
GenomeRNAiⁱ	5646
Pharosⁱ	P35030, Tchem
Protein Ontology More...PROⁱ	PR:P35030
RNActⁱ	P35030, protein
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000010438, Expressed in pancreas and 184 other tissues
ExpressionAtlasⁱ	P35030, baseline and differential
Genevisibleⁱ	P35030, HS

Family and domain databases

CDDⁱ	cd00190, Tryp_SPc, 1 hit
Gene3Dⁱ	2.40.10.10, 2 hits
InterProⁱ	View protein in InterPro IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS
Pfamⁱ	View protein in Pfam PF00089, Trypsin, 1 hit
PRINTSⁱ	PR00722, CHYMOTRYPSIN
SMARTⁱ	View protein in SMART SM00020, Tryp_SPc, 1 hit
SUPFAMⁱ	SSF50494, SSF50494, 1 hit
PROSITEⁱ	View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit
MobiDBⁱ	Search...

Entry informationⁱ

Entry nameⁱ	TRY3_HUMAN
Accessionⁱ	P35030Primary (citable) accession number: P35030 Secondary accession number(s): A8CED1 Q9UQV3
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
	Last sequence update:	October 14, 2008
	Last modified:	September 29, 2021
	This is version 206 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Reference proteome

Documents

Peptidase families
Classification of peptidase families and list of entries
Human chromosome 9
Human chromosome 9: entries, gene names and cross-references to MIM
Human entries with genetic variants
List of human entries with genetic variants
Human variants curated from literature reports
Index of human variants curated from literature reports
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
SIMILARITY comments
Index of protein domains and families

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UniProtKB - P35030 (TRY3_HUMAN)

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Trypsin-3

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>pH dependencei

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GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted

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Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

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<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

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<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

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<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

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Family and domain databases

Experimental Info

Natural variant

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

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Functionⁱ

Catalytic activityⁱ

pH dependenceⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ