UniProtKB - P34980 (PE2R3_RAT)
Prostaglandin E2 receptor EP3 subtype
Ptger3
Functioni
Receptor for prostaglandin E2 (PGE2) (PubMed:8393672, PubMed:11006128).
Required for normal development of fever in response to pyrinogens, including IL1B, prostaglandin E2 and bacterial lipopolysaccharide (LPS). Required for normal potentiation of platelet aggregation by prostaglandin E2, and thus plays a role in the regulation of blood coagulation. Required for increased HCO3- secretion in the duodenum in response to mucosal acidification, and thereby contributes to the protection of the mucosa against acid-induced ulceration. Not required for normal kidney function, normal urine volume and osmolality (By similarity).
By similarity2 PublicationsReceptor for prostaglandin E2 (PGE2); ligand binding activates a signaling cascade via G(i) proteins that leads to the inhibition of adenylate cyclase.
1 PublicationReceptor for prostaglandin E2 (PGE2); ligand binding can activate several distinct signaling cascades, resulting in activation or inhibition of adenylate cyclase.
By similarityGO - Molecular functioni
- prostaglandin E receptor activity Source: RGD
GO - Biological processi
- adenylate cyclase-activating G protein-coupled receptor signaling pathway Source: GO_Central
- adenylate cyclase-modulating G protein-coupled receptor signaling pathway Source: RGD
- bicarbonate transport Source: RGD
- cellular response to forskolin Source: RGD
- cellular response to interleukin-1 Source: RGD
- cellular response to lipopolysaccharide Source: RGD
- desensitization of G protein-coupled receptor signaling pathway Source: RGD
- female pregnancy Source: RGD
- fever generation Source: RGD
- inflammatory response Source: GO_Central
- intestine smooth muscle contraction Source: RGD
- maternal process involved in parturition Source: RGD
- negative regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway Source: RGD
- negative regulation of calcium ion transmembrane transport Source: RGD
- negative regulation of circadian sleep/wake cycle, wakefulness Source: RGD
- negative regulation of forebrain neuron differentiation Source: RGD
- negative regulation of gastric acid secretion Source: RGD
- negative regulation of gastrin-induced gastric acid secretion Source: RGD
- negative regulation of insulin secretion Source: RGD
- negative regulation of norepinephrine secretion Source: RGD
- negative regulation of platelet aggregation Source: RGD
- negative regulation of potassium ion transmembrane transport Source: RGD
- phospholipase C-activating G protein-coupled receptor signaling pathway Source: RGD
- positive regulation of cAMP-mediated signaling Source: RGD
- positive regulation of circadian sleep/wake cycle, non-REM sleep Source: RGD
- positive regulation of colon smooth muscle contraction Source: RGD
- positive regulation of cytosolic calcium ion concentration Source: RGD
- positive regulation of fever generation Source: RGD
- positive regulation of gastric mucosal blood circulation Source: RGD
- positive regulation of gene expression Source: RGD
- positive regulation of inhibitory G protein-coupled receptor phosphorylation Source: RGD
- positive regulation of myofibroblast contraction Source: RGD
- positive regulation of neutrophil extravasation Source: RGD
- positive regulation of protein phosphorylation Source: RGD
- positive regulation of smooth muscle contraction involved in micturition Source: RGD
- positive regulation of urine volume Source: RGD
- positive regulation of vasoconstriction Source: RGD
- prostaglandin receptor internalization Source: RGD
- receptor internalization Source: RGD
- regulation of circadian sleep/wake cycle, wakefulness Source: RGD
- regulation of norepinephrine secretion Source: RGD
- response to estradiol Source: RGD
- response to lipopolysaccharide Source: RGD
- response to progesterone Source: RGD
- response to salt Source: RGD
- response to xenobiotic stimulus Source: RGD
- urinary bladder smooth muscle contraction Source: RGD
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Enzyme and pathway databases
Reactomei | R-RNO-391908, Prostanoid ligand receptors R-RNO-418594, G alpha (i) signalling events |
Names & Taxonomyi
Protein namesi | Recommended name: Prostaglandin E2 receptor EP3 subtypeShort name: PGE receptor EP3 subtype Short name: PGE2 receptor EP3 subtype Alternative name(s): Prostanoid EP3 receptor |
Gene namesi | Name:Ptger3 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 3435, Ptger3 |
Subcellular locationi
Plasma membrane
- Cell membrane 2 Publications; Multi-pass membrane protein Curated
Nucleus
- nuclear membrane Source: RGD
Plasma Membrane
- brush border membrane Source: RGD
- plasma membrane Source: RGD
Other locations
- cell surface Source: RGD
- integral component of membrane Source: UniProtKB-KW
- neuron projection Source: RGD
- neuronal cell body Source: RGD
- proximal neuron projection Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 30 | ExtracellularSequence analysisAdd BLAST | 30 | |
Transmembranei | 31 – 55 | Helical; Name=1Sequence analysisAdd BLAST | 25 | |
Topological domaini | 56 – 68 | CytoplasmicSequence analysisAdd BLAST | 13 | |
Transmembranei | 69 – 89 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 90 – 108 | ExtracellularSequence analysisAdd BLAST | 19 | |
Transmembranei | 109 – 130 | Helical; Name=3Sequence analysisAdd BLAST | 22 | |
Topological domaini | 131 – 151 | CytoplasmicSequence analysisAdd BLAST | 21 | |
Transmembranei | 152 – 173 | Helical; Name=4Sequence analysisAdd BLAST | 22 | |
Topological domaini | 174 – 203 | ExtracellularSequence analysisAdd BLAST | 30 | |
Transmembranei | 204 – 229 | Helical; Name=5Sequence analysisAdd BLAST | 26 | |
Topological domaini | 230 – 259 | CytoplasmicSequence analysisAdd BLAST | 30 | |
Transmembranei | 260 – 283 | Helical; Name=6Sequence analysisAdd BLAST | 24 | |
Topological domaini | 284 – 303 | ExtracellularSequence analysisAdd BLAST | 20 | |
Transmembranei | 304 – 325 | Helical; Name=7Sequence analysisAdd BLAST | 22 | |
Topological domaini | 326 – 365 | CytoplasmicSequence analysisAdd BLAST | 40 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Chemistry databases
ChEMBLi | CHEMBL5674 |
DrugCentrali | P34980 |
GuidetoPHARMACOLOGYi | 342 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000070062 | 1 – 365 | Prostaglandin E2 receptor EP3 subtypeAdd BLAST | 365 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 16 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 107 ↔ 184 | PROSITE-ProRule annotation | ||
Glycosylationi | 193 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, Lipoprotein, PalmitateProteomic databases
PaxDbi | P34980 |
PTM databases
GlyGeni | P34980, 2 sites |
PhosphoSitePlusi | P34980 |
Expressioni
Tissue specificityi
Interactioni
Subunit structurei
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000014034 |
Chemistry databases
BindingDBi | P34980 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3656, Eukaryota |
InParanoidi | P34980 |
PhylomeDBi | P34980 |
Family and domain databases
InterProi | View protein in InterPro IPR000154, EP3_rcpt_3 IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR008365, Prostanoid_rcpt IPR001244, Prostglndn_DP_rcpt IPR000265, Prostglndn_EP3_rcpt |
PANTHERi | PTHR11866, PTHR11866, 1 hit PTHR11866:SF10, PTHR11866:SF10, 1 hit |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN PR00428, PROSTAGLNDNR PR01788, PROSTANOIDR PR00585, PRSTNOIDE33R PR00582, PRSTNOIDEP3R |
PROSITEi | View protein in PROSITE PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAGVWAPEHS VEAHSNQSSA ADGCGSVSVA FPITMMVTGF VGNALAMLLV
60 70 80 90 100
VRSYRRRESK RKKSFLLCIG WLALTDLVGQ LLTSPVVILV YLSQRRWEQL
110 120 130 140 150
DPSGRLCTFF GLTMTVFGLS SLLVASAMAV ERALAIRAPH WYASHMKTRA
160 170 180 190 200
TPVLLGVWLS VLAFALLPVL GVGRYSVQWP GTWCFISTGP AGNETDSARE
210 220 230 240 250
PGSVAFASAF ACLGLLALVV TFACNLATIK ALVSRCRAKA AASQSSAQWG
260 270 280 290 300
RITTETAIQL MGIMCVLSVC WSPLLIMMLK MIFNQMSVEQ CKTQMGKEKE
310 320 330 340 350
CNSFLIAVRL ASLNQILDPW VYLLLRKILL RKFCQIRDHT NYASSSTSLP
360
CPGSSVLMWS DQLER
The sequence of this isoform differs from the canonical sequence as follows:
336-365: IRDHTNYASSSTSLPCPGSSVLMWSDQLER → MMNNLKRSFIAIPASLSMRISSPREG
The sequence of this isoform differs from the canonical sequence as follows:
336-365: IRDHTNYASSSTSLPCPGSSVLMWSDQLER → VANAVSSCSSDQQKGQAISLSNEVVHPGP
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG3V7D9 | G3V7D9_RAT | Prostaglandin E2 receptor EP3 subty... | Ptger3 rCG_28575 | 366 | Annotation score: | ||
A0A0G2JSK4 | A0A0G2JSK4_RAT | Prostaglandin E2 receptor EP3 subty... | Ptger3 rCG_28575 | 362 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 51 | V → S (PubMed:8076679).Curated | 1 | |
Sequence conflicti | 51 | V → S (PubMed:8919306).Curated | 1 | |
Sequence conflicti | 354 | S → F in CAA58735 (PubMed:8076679).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 152 | P → RA. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_001949 | 336 – 365 | IRDHT…DQLER → MMNNLKRSFIAIPASLSMRI SSPREG in isoform Beta. 2 PublicationsAdd BLAST | 30 | |
Alternative sequenceiVSP_001950 | 336 – 365 | IRDHT…DQLER → VANAVSSCSSDQQKGQAISL SNEVVHPGP in isoform Gamma. 1 PublicationAdd BLAST | 30 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D14869 mRNA Translation: BAA03585.1 D16443 mRNA Translation: BAA03912.1 X80133 mRNA Translation: CAA56432.1 D29969 mRNA Translation: BAA06236.1 X83855 mRNA Translation: CAA58735.1 |
PIRi | JN0693 S48689 S51280 |
RefSeqi | NP_036836.1, NM_012704.1 |
Genome annotation databases
GeneIDi | 24929 |
KEGGi | rno:24929 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D14869 mRNA Translation: BAA03585.1 D16443 mRNA Translation: BAA03912.1 X80133 mRNA Translation: CAA56432.1 D29969 mRNA Translation: BAA06236.1 X83855 mRNA Translation: CAA58735.1 |
PIRi | JN0693 S48689 S51280 |
RefSeqi | NP_036836.1, NM_012704.1 |
3D structure databases
SMRi | P34980 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000014034 |
Chemistry databases
BindingDBi | P34980 |
ChEMBLi | CHEMBL5674 |
DrugCentrali | P34980 |
GuidetoPHARMACOLOGYi | 342 |
Protein family/group databases
GPCRDBi | Search... |
PTM databases
GlyGeni | P34980, 2 sites |
PhosphoSitePlusi | P34980 |
Proteomic databases
PaxDbi | P34980 |
Protocols and materials databases
DNASUi | 24929 |
Genome annotation databases
GeneIDi | 24929 |
KEGGi | rno:24929 |
Organism-specific databases
CTDi | 5733 |
RGDi | 3435, Ptger3 |
Phylogenomic databases
eggNOGi | KOG3656, Eukaryota |
InParanoidi | P34980 |
PhylomeDBi | P34980 |
Enzyme and pathway databases
Reactomei | R-RNO-391908, Prostanoid ligand receptors R-RNO-418594, G alpha (i) signalling events |
Miscellaneous databases
PROi | PR:P34980 |
Family and domain databases
InterProi | View protein in InterPro IPR000154, EP3_rcpt_3 IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR008365, Prostanoid_rcpt IPR001244, Prostglndn_DP_rcpt IPR000265, Prostglndn_EP3_rcpt |
PANTHERi | PTHR11866, PTHR11866, 1 hit PTHR11866:SF10, PTHR11866:SF10, 1 hit |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN PR00428, PROSTAGLNDNR PR01788, PROSTANOIDR PR00585, PRSTNOIDE33R PR00582, PRSTNOIDEP3R |
PROSITEi | View protein in PROSITE PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PE2R3_RAT | |
Accessioni | P34980Primary (citable) accession number: P34980 Secondary accession number(s): Q63194, Q64376 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | February 1, 1994 | |
Last modified: | February 23, 2022 | |
This is version 159 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries - SIMILARITY comments
Index of protein domains and families