UniProtKB - P34979 (PE2R3_BOVIN)
Prostaglandin E2 receptor EP3 subtype
PTGER3
Functioni
Receptor for prostaglandin E2 (PGE2) (PubMed:8396726).
The various isoforms have identical ligand binding properties but interact with different second messenger systems: isoform EP3A couples to G(i)/G(o) proteins; isoform EP3B and isoform EP3C couple to G(s), and isoform EP3D couples to G(i), G(s) and G(p) (PubMed:8396726).
Required for normal development of fever in response to pyrinogens, including IL1B, prostaglandin E2 and bacterial lipopolysaccharide (LPS). Required for normal potentiation of platelet aggregation by prostaglandin E2, and thus plays a role in the regulation of blood coagulation. Required for increased HCO3- secretion in the duodenum in response to mucosal acidification, and thereby contributes to the protection of the mucosa against acid-induced ulceration. Not required for normal kidney function, normal urine volume and osmolality (By similarity).
By similarity1 PublicationGO - Molecular functioni
- prostaglandin E receptor activity Source: GO_Central
GO - Biological processi
- adenylate cyclase-activating G protein-coupled receptor signaling pathway Source: GO_Central
- inflammatory response Source: GO_Central
- intestine smooth muscle contraction Source: GO_Central
- negative regulation of gastric acid secretion Source: GO_Central
- phospholipase C-activating G protein-coupled receptor signaling pathway Source: GO_Central
- positive regulation of cytosolic calcium ion concentration Source: GO_Central
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Names & Taxonomyi
Protein namesi | Recommended name: Prostaglandin E2 receptor EP3 subtypeShort name: PGE receptor EP3 subtype Short name: PGE2 receptor EP3 subtype Alternative name(s): Prostanoid EP3 receptor |
Gene namesi | Name:PTGER3 |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | HostDB:ENSBTAG00000019230 |
VGNCi | VGNC:33503, PTGER3 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein Curated
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 52 | ExtracellularSequence analysisAdd BLAST | 52 | |
Transmembranei | 53 – 77 | Helical; Name=1Sequence analysisAdd BLAST | 25 | |
Topological domaini | 78 – 90 | CytoplasmicSequence analysisAdd BLAST | 13 | |
Transmembranei | 91 – 111 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 112 – 130 | ExtracellularSequence analysisAdd BLAST | 19 | |
Transmembranei | 131 – 152 | Helical; Name=3Sequence analysisAdd BLAST | 22 | |
Topological domaini | 153 – 174 | CytoplasmicSequence analysisAdd BLAST | 22 | |
Transmembranei | 175 – 196 | Helical; Name=4Sequence analysisAdd BLAST | 22 | |
Topological domaini | 197 – 226 | ExtracellularSequence analysisAdd BLAST | 30 | |
Transmembranei | 227 – 252 | Helical; Name=5Sequence analysisAdd BLAST | 26 | |
Topological domaini | 253 – 282 | CytoplasmicSequence analysisAdd BLAST | 30 | |
Transmembranei | 283 – 306 | Helical; Name=6Sequence analysisAdd BLAST | 24 | |
Topological domaini | 307 – 326 | ExtracellularSequence analysisAdd BLAST | 20 | |
Transmembranei | 327 – 348 | Helical; Name=7Sequence analysisAdd BLAST | 22 | |
Topological domaini | 349 – 417 | CytoplasmicSequence analysisAdd BLAST | 69 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000070057 | 1 – 417 | Prostaglandin E2 receptor EP3 subtypeAdd BLAST | 417 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 17 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 35 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 216 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 307 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Glycoprotein, Lipoprotein, PalmitateProteomic databases
PaxDbi | P34979 |
Expressioni
Gene expression databases
Bgeei | ENSBTAG00000019230, Expressed in heart and 80 other tissues |
ExpressionAtlasi | P34979, baseline and differential |
Interactioni
Subunit structurei
Interacts (via C-terminus) with MKLN1.
By similarityProtein-protein interaction databases
STRINGi | 9913.ENSBTAP00000025607 |
Chemistry databases
BindingDBi | P34979 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3656, Eukaryota |
GeneTreei | ENSGT01030000234559 |
HOGENOMi | CLU_045991_3_0_1 |
InParanoidi | P34979 |
OMAi | PITMMIT |
OrthoDBi | 972015at2759 |
TreeFami | TF324982 |
Family and domain databases
InterProi | View protein in InterPro IPR003304, EP3_rcpt_1 IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR008365, Prostanoid_rcpt IPR001244, Prostglndn_DP_rcpt IPR000265, Prostglndn_EP3_rcpt |
PANTHERi | PTHR11866, PTHR11866, 1 hit PTHR11866:SF10, PTHR11866:SF10, 1 hit |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN PR00428, PROSTAGLNDNR PR01788, PROSTANOIDR PR00583, PRSTNOIDE31R PR00582, PRSTNOIDEP3R |
PROSITEi | View protein in PROSITE PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MKATRDHASA PFCTRFNHSD PGIWAAERAV EAPNNLTLPP EPSEDCGSVS
60 70 80 90 100
VAFSMTMMIT GFVGNALAIT LVSKSYRRRE GKRKKSFLLC IGWLALTDMV
110 120 130 140 150
GQLLTSPVVI VLYLSHQRWE QLDPSGRLCT FFGLTMTVFG LSSLFIASAM
160 170 180 190 200
AVERALATRA PHWYSSHMKT SVTRAVLLGV WLAVLAFALL PVLGVGQYTI
210 220 230 240 250
QWPGTWCFIS TGPGGNGTNS RQNWGNVFFA SAFAILGLSA LVVTFACNLA
260 270 280 290 300
TIKALVSRCR AKATASQSSA QWGRITTETA IQLMGIMCVL SVCWSPLLIM
310 320 330 340 350
MLKMIFNHTS VEHCKTYTEN QDECNFFLIA VRLASLNQIL DPWVYLLLRK
360 370 380 390 400
ILLQKFCQLL KGHSYGLDTE GGTENKDKEM KENLYISNLS RFFILLGHFT
410
EARRGRGHIY LHTLEHQ
The sequence of this isoform differs from the canonical sequence as follows:
359-387: LLKGHSYGLDTEGGTENKDKEMKENLYIS → VANAVSSYFNDGPKVPTISLSNEITQTGA
388-417: Missing.
The sequence of this isoform differs from the canonical sequence as follows:
359-362: LLKG → HVGS
363-417: Missing.
The sequence of this isoform differs from the canonical sequence as follows:
359-385: LLKGHSYGLDTEGGTENKDKEMKENLY → ASPRSMWDPSSPTRDRTRVPCIGSTES
386-417: Missing.
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG3MXS8 | G3MXS8_BOVIN | Prostaglandin E2 receptor EP3 subty... | PTGER3 | 378 | Annotation score: | ||
A0A3Q1LRX6 | A0A3Q1LRX6_BOVIN | Prostaglandin E2 receptor EP3 subty... | PTGER3 | 418 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_001929 | 359 – 387 | LLKGH…NLYIS → VANAVSSYFNDGPKVPTISL SNEITQTGA in isoform EP3B. 1 PublicationAdd BLAST | 29 | |
Alternative sequenceiVSP_001933 | 359 – 385 | LLKGH…KENLY → ASPRSMWDPSSPTRDRTRVP CIGSTES in isoform EP3D. 1 PublicationAdd BLAST | 27 | |
Alternative sequenceiVSP_001931 | 359 – 362 | LLKG → HVGS in isoform EP3C. 1 Publication | 4 | |
Alternative sequenceiVSP_001932 | 363 – 417 | Missing in isoform EP3C. 1 PublicationAdd BLAST | 55 | |
Alternative sequenceiVSP_001934 | 386 – 417 | Missing in isoform EP3D. 1 PublicationAdd BLAST | 32 | |
Alternative sequenceiVSP_001930 | 388 – 417 | Missing in isoform EP3B. 1 PublicationAdd BLAST | 30 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D21345 mRNA Translation: BAA04811.1 D21346 mRNA Translation: BAA04812.1 D21347 mRNA Translation: BAA04813.1 D21348 mRNA Translation: BAA04814.1 |
PIRi | S36764 S36765 S36766 S36767 |
RefSeqi | NP_851375.1, NM_181032.1 [P34979-1] XP_005204501.1, XM_005204444.3 [P34979-3] XP_010801729.1, XM_010803427.2 |
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D21345 mRNA Translation: BAA04811.1 D21346 mRNA Translation: BAA04812.1 D21347 mRNA Translation: BAA04813.1 D21348 mRNA Translation: BAA04814.1 |
PIRi | S36764 S36765 S36766 S36767 |
RefSeqi | NP_851375.1, NM_181032.1 [P34979-1] XP_005204501.1, XM_005204444.3 [P34979-3] XP_010801729.1, XM_010803427.2 |
3D structure databases
SMRi | P34979 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000025607 |
Chemistry databases
BindingDBi | P34979 |
Protein family/group databases
GPCRDBi | Search... |
Proteomic databases
PaxDbi | P34979 |
Genome annotation databases
Organism-specific databases
CTDi | 5733 |
VEuPathDBi | HostDB:ENSBTAG00000019230 |
VGNCi | VGNC:33503, PTGER3 |
Phylogenomic databases
eggNOGi | KOG3656, Eukaryota |
GeneTreei | ENSGT01030000234559 |
HOGENOMi | CLU_045991_3_0_1 |
InParanoidi | P34979 |
OMAi | PITMMIT |
OrthoDBi | 972015at2759 |
TreeFami | TF324982 |
Gene expression databases
Bgeei | ENSBTAG00000019230, Expressed in heart and 80 other tissues |
ExpressionAtlasi | P34979, baseline and differential |
Family and domain databases
InterProi | View protein in InterPro IPR003304, EP3_rcpt_1 IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR008365, Prostanoid_rcpt IPR001244, Prostglndn_DP_rcpt IPR000265, Prostglndn_EP3_rcpt |
PANTHERi | PTHR11866, PTHR11866, 1 hit PTHR11866:SF10, PTHR11866:SF10, 1 hit |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN PR00428, PROSTAGLNDNR PR01788, PROSTANOIDR PR00583, PRSTNOIDE31R PR00582, PRSTNOIDEP3R |
PROSITEi | View protein in PROSITE PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PE2R3_BOVIN | |
Accessioni | P34979Primary (citable) accession number: P34979 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | February 1, 1994 | |
Last modified: | February 23, 2022 | |
This is version 154 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries - SIMILARITY comments
Index of protein domains and families