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Protein

Heat shock 70 kDa protein 1-like

Gene

HSPA1L

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The affinity for polypeptides is regulated by its nucleotide bound state. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release (PubMed:26865365). Positive regulator of PRKN translocation to damaged mitochondria (PubMed:24270810).1 Publication1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei73ATP1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 17ATP4
Nucleotide bindingi204 – 206ATP3
Nucleotide bindingi270 – 277ATP8
Nucleotide bindingi341 – 344ATP4

GO - Molecular functioni

  • ATPase activity Source: GO_Central
  • ATPase activity, coupled Source: GO_Central
  • ATP binding Source: GO_Central
  • heat shock protein binding Source: UniProtKB
  • misfolded protein binding Source: GO_Central
  • protein binding involved in protein folding Source: GO_Central
  • ubiquitin protein ligase binding Source: ParkinsonsUK-UCL
  • unfolded protein binding Source: UniProtKB

GO - Biological processi

Keywordsi

LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-3371453 Regulation of HSF1-mediated heat shock response
R-HSA-3371497 HSP90 chaperone cycle for steroid hormone receptors (SHR)
R-HSA-3371568 Attenuation phase
R-HSA-3371571 HSF1-dependent transactivation

Names & Taxonomyi

Protein namesi
Recommended name:
Heat shock 70 kDa protein 1-like
Short name:
Heat shock 70 kDa protein 1L
Alternative name(s):
Heat shock 70 kDa protein 1-Hom
Short name:
HSP70-Hom
Gene namesi
Name:HSPA1L
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000204390.9
HGNCiHGNC:5234 HSPA1L
MIMi140559 gene
neXtProtiNX_P34931

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi73K → E: No rescue of PRKN translocation deficit in knockout cells. 1 Publication1
Mutagenesisi396L → D: No rescue of PRKN translocation deficit in knockout cells. 1 Publication1
Mutagenesisi638 – 641Missing : No rescue of PRKN translocation deficit in knockout cells. 1 Publication4

Organism-specific databases

DisGeNETi3305
OpenTargetsiENSG00000204390
PharmGKBiPA29500

Polymorphism and mutation databases

BioMutaiHSPA1L
DMDMi23831140

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000782551 – 641Heat shock 70 kDa protein 1-likeAdd BLAST641

Proteomic databases

EPDiP34931
MaxQBiP34931
PaxDbiP34931
PeptideAtlasiP34931
PRIDEiP34931
ProteomicsDBi54956
TopDownProteomicsiP34931

2D gel databases

UCD-2DPAGEiP34931

PTM databases

GlyConnecti1297
iPTMnetiP34931
PhosphoSitePlusiP34931
SwissPalmiP34931

Expressioni

Tissue specificityi

Expressed in spermatids.1 Publication

Inductioni

Not induced by heat shock.1 Publication

Gene expression databases

BgeeiENSG00000204390 Expressed in 163 organ(s), highest expression level in sperm
CleanExiHS_HSPA1L
ExpressionAtlasiP34931 baseline and differential
GenevisibleiP34931 HS

Organism-specific databases

HPAiHPA043285
HPA052504

Interactioni

Subunit structurei

Interacts with PRKN.2 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi109537, 172 interactors
ELMiP34931
IntActiP34931, 57 interactors
MINTiP34931
STRINGi9606.ENSP00000364805

Structurei

Secondary structure

1641
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP34931
SMRiP34931
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP34931

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni3 – 388Nucleotide-binding domain (NBD)By similarityAdd BLAST386
Regioni396 – 511Substrate-binding domain (SBD)By similarityAdd BLAST116

Domaini

The N-terminal nucleotide binding domain (NBD) (also known as the ATPase domain) is responsible for binding and hydrolyzing ATP. The C-terminal substrate-binding domain (SBD) (also known as peptide-binding domain) binds to the client/substrate proteins. The two domains are allosterically coupled so that, when ATP is bound to the NBD, the SBD binds relatively weakly to clients. When ADP is bound in the NBD, a conformational change enhances the affinity of the SBD for client proteins.1 Publication

Sequence similaritiesi

Belongs to the heat shock protein 70 family.Curated

Phylogenomic databases

eggNOGiKOG0101 Eukaryota
COG0443 LUCA
GeneTreeiENSGT00930000150862
HOVERGENiHBG051845
InParanoidiP34931
KOiK03283
OMAiGEKKAFY
OrthoDBiEOG091G03SF
PhylomeDBiP34931
TreeFamiTF105042

Family and domain databases

Gene3Di1.20.1270.10, 1 hit
2.60.34.10, 1 hit
InterProiView protein in InterPro
IPR018181 Heat_shock_70_CS
IPR029048 HSP70_C_sf
IPR029047 HSP70_peptide-bd_sf
IPR013126 Hsp_70_fam
PANTHERiPTHR19375 PTHR19375, 1 hit
PfamiView protein in Pfam
PF00012 HSP70, 1 hit
PRINTSiPR00301 HEATSHOCK70
SUPFAMiSSF100920 SSF100920, 1 hit
SSF100934 SSF100934, 1 hit
PROSITEiView protein in PROSITE
PS00297 HSP70_1, 1 hit
PS00329 HSP70_2, 1 hit
PS01036 HSP70_3, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P34931-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MATAKGIAIG IDLGTTYSCV GVFQHGKVEI IANDQGNRTT PSYVAFTDTE
60 70 80 90 100
RLIGDAAKNQ VAMNPQNTVF DAKRLIGRKF NDPVVQADMK LWPFQVINEG
110 120 130 140 150
GKPKVLVSYK GENKAFYPEE ISSMVLTKLK ETAEAFLGHP VTNAVITVPA
160 170 180 190 200
YFNDSQRQAT KDAGVIAGLN VLRIINEPTA AAIAYGLDKG GQGERHVLIF
210 220 230 240 250
DLGGGTFDVS ILTIDDGIFE VKATAGDTHL GGEDFDNRLV SHFVEEFKRK
260 270 280 290 300
HKKDISQNKR AVRRLRTACE RAKRTLSSST QANLEIDSLY EGIDFYTSIT
310 320 330 340 350
RARFEELCAD LFRGTLEPVE KALRDAKMDK AKIHDIVLVG GSTRIPKVQR
360 370 380 390 400
LLQDYFNGRD LNKSINPDEA VAYGAAVQAA ILMGDKSEKV QDLLLLDVAP
410 420 430 440 450
LSLGLETAGG VMTALIKRNS TIPTKQTQIF TTYSDNQPGV LIQVYEGERA
460 470 480 490 500
MTKDNNLLGR FDLTGIPPAP RGVPQIEVTF DIDANGILNV TATDKSTGKV
510 520 530 540 550
NKITITNDKG RLSKEEIERM VLDAEKYKAE DEVQREKIAA KNALESYAFN
560 570 580 590 600
MKSVVSDEGL KGKISESDKN KILDKCNELL SWLEVNQLAE KDEFDHKRKE
610 620 630 640
LEQMCNPIIT KLYQGGCTGP ACGTGYVPGR PATGPTIEEV D
Length:641
Mass (Da):70,375
Last modified:October 10, 2002 - v2
Checksum:iA9339D7657166FF7
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q53FA3Q53FA3_HUMAN
HSPA1L
HSPA1L
641Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti408A → V in AAA63228 (PubMed:1700760).Curated1
Sequence conflicti416I → M in AAH34483 (PubMed:15489334).Curated1
Sequence conflicti424T → P in AAA63228 (PubMed:1700760).Curated1
Sequence conflicti506T → A in AAH34483 (PubMed:15489334).Curated1
Sequence conflicti627V → M in BAA32521 (PubMed:9685725).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0258418A → P1 PublicationCorresponds to variant dbSNP:rs9469057Ensembl.1
Natural variantiVAR_025842268A → T1 PublicationCorresponds to variant dbSNP:rs34620296EnsemblClinVar.1
Natural variantiVAR_025843294D → G1 PublicationCorresponds to variant dbSNP:rs34360259Ensembl.1
Natural variantiVAR_025844479T → M1 PublicationCorresponds to variant dbSNP:rs482145Ensembl.1
Natural variantiVAR_003820493T → M4 PublicationsCorresponds to variant dbSNP:rs2227956Ensembl.1
Natural variantiVAR_025845558E → A1 PublicationCorresponds to variant dbSNP:rs2227955EnsemblClinVar.1
Natural variantiVAR_025846602E → K1 PublicationCorresponds to variant dbSNP:rs2075800Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59829 Genomic DNA Translation: AAA63228.1
D85730 mRNA Translation: BAA32521.1
AF134726 Genomic DNA Translation: AAD21817.1
BA000025 Genomic DNA Translation: BAB63301.1
DQ383515 Genomic DNA Translation: ABC88476.1
AL662834 Genomic DNA No translation available.
AL671762 Genomic DNA No translation available.
AL929592 Genomic DNA No translation available.
CR388202 Genomic DNA No translation available.
BC034483 mRNA Translation: AAH34483.1
CCDSiCCDS34413.1
PIRiB45871
RefSeqiNP_005518.3, NM_005527.3
UniGeneiHs.690634

Genome annotation databases

EnsembliENST00000375654; ENSP00000364805; ENSG00000204390
ENST00000383390; ENSP00000372881; ENSG00000206383
ENST00000417601; ENSP00000396486; ENSG00000234258
ENST00000456772; ENSP00000408347; ENSG00000236251
GeneIDi3305
KEGGihsa:3305
UCSCiuc003nxh.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59829 Genomic DNA Translation: AAA63228.1
D85730 mRNA Translation: BAA32521.1
AF134726 Genomic DNA Translation: AAD21817.1
BA000025 Genomic DNA Translation: BAB63301.1
DQ383515 Genomic DNA Translation: ABC88476.1
AL662834 Genomic DNA No translation available.
AL671762 Genomic DNA No translation available.
AL929592 Genomic DNA No translation available.
CR388202 Genomic DNA No translation available.
BC034483 mRNA Translation: AAH34483.1
CCDSiCCDS34413.1
PIRiB45871
RefSeqiNP_005518.3, NM_005527.3
UniGeneiHs.690634

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3GDQX-ray1.80A1-386[»]
ProteinModelPortaliP34931
SMRiP34931
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109537, 172 interactors
ELMiP34931
IntActiP34931, 57 interactors
MINTiP34931
STRINGi9606.ENSP00000364805

PTM databases

GlyConnecti1297
iPTMnetiP34931
PhosphoSitePlusiP34931
SwissPalmiP34931

Polymorphism and mutation databases

BioMutaiHSPA1L
DMDMi23831140

2D gel databases

UCD-2DPAGEiP34931

Proteomic databases

EPDiP34931
MaxQBiP34931
PaxDbiP34931
PeptideAtlasiP34931
PRIDEiP34931
ProteomicsDBi54956
TopDownProteomicsiP34931

Protocols and materials databases

DNASUi3305
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000375654; ENSP00000364805; ENSG00000204390
ENST00000383390; ENSP00000372881; ENSG00000206383
ENST00000417601; ENSP00000396486; ENSG00000234258
ENST00000456772; ENSP00000408347; ENSG00000236251
GeneIDi3305
KEGGihsa:3305
UCSCiuc003nxh.4 human

Organism-specific databases

CTDi3305
DisGeNETi3305
EuPathDBiHostDB:ENSG00000204390.9
GeneCardsiHSPA1L
H-InvDBiHIX0165969
HIX0166127
HIX0166446
HIX0167182
HIX0184230
HGNCiHGNC:5234 HSPA1L
HPAiHPA043285
HPA052504
MIMi140559 gene
neXtProtiNX_P34931
OpenTargetsiENSG00000204390
PharmGKBiPA29500
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0101 Eukaryota
COG0443 LUCA
GeneTreeiENSGT00930000150862
HOVERGENiHBG051845
InParanoidiP34931
KOiK03283
OMAiGEKKAFY
OrthoDBiEOG091G03SF
PhylomeDBiP34931
TreeFamiTF105042

Enzyme and pathway databases

ReactomeiR-HSA-3371453 Regulation of HSF1-mediated heat shock response
R-HSA-3371497 HSP90 chaperone cycle for steroid hormone receptors (SHR)
R-HSA-3371568 Attenuation phase
R-HSA-3371571 HSF1-dependent transactivation

Miscellaneous databases

EvolutionaryTraceiP34931
GeneWikiiHSPA1L
GenomeRNAii3305
PROiPR:P34931
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000204390 Expressed in 163 organ(s), highest expression level in sperm
CleanExiHS_HSPA1L
ExpressionAtlasiP34931 baseline and differential
GenevisibleiP34931 HS

Family and domain databases

Gene3Di1.20.1270.10, 1 hit
2.60.34.10, 1 hit
InterProiView protein in InterPro
IPR018181 Heat_shock_70_CS
IPR029048 HSP70_C_sf
IPR029047 HSP70_peptide-bd_sf
IPR013126 Hsp_70_fam
PANTHERiPTHR19375 PTHR19375, 1 hit
PfamiView protein in Pfam
PF00012 HSP70, 1 hit
PRINTSiPR00301 HEATSHOCK70
SUPFAMiSSF100920 SSF100920, 1 hit
SSF100934 SSF100934, 1 hit
PROSITEiView protein in PROSITE
PS00297 HSP70_1, 1 hit
PS00329 HSP70_2, 1 hit
PS01036 HSP70_3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiHS71L_HUMAN
AccessioniPrimary (citable) accession number: P34931
Secondary accession number(s): A6NNB0
, B0UXW8, O75634, Q2HXR3, Q8NE72, Q96QC9, Q9UQM1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: October 10, 2002
Last modified: November 7, 2018
This is version 179 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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