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Protein

Glutathione transferase omega-1

Gene

gsto-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Exhibits glutathione-dependent thiol transferase activity (By similarity). Has dehydroascorbate reductase activity and may contribute to the recycling of ascorbic acid (By similarity). Participates in the biotransformation of inorganic arsenic and reduces monomethylarsonic acid (MMA) (By similarity). Protects against environmental stress and oxidative stress (PubMed:17901115).By similarity1 Publication

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.By similarity
2 glutathione + dehydroascorbate = glutathione disulfide + ascorbate.1 Publication
Methylarsonate + 2 glutathione = methylarsonite + glutathione disulfide + H2O.1 Publication

Kineticsi

  1. KM=51.52 µM for 1-chloro-2,4-dintrobenzene1 Publication
  2. KM=184.1 µM for dehydroascorbate1 Publication
  3. KM=70.77 µM for 2-hydroxyethyl disulfide1 Publication
  1. Vmax=106 nmol/min/mg enzyme towards 1-chloro-2,4-dintrobenzene1 Publication
  2. Vmax=32 nmol/min/mg enzyme towards cumene hydroperoxide1 Publication
  3. Vmax=186 nmol/min/mg enzyme towards dehydroascorbate1 Publication
  4. Vmax=192 nmol/min/mg enzyme towards 2-hydroxyethyl disulfide1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei33NucleophileBy similarity1
Binding sitei60GlutathioneBy similarity1

GO - Molecular functioni

  • glutathione dehydrogenase (ascorbate) activity Source: UniProtKB
  • glutathione transferase activity Source: UniProtKB
  • methylarsonate reductase activity Source: UniProtKB-EC

GO - Biological processi

  • cellular response to extracellular stimulus Source: UniProtKB
  • glutathione metabolic process Source: GO_Central
  • oxidation-reduction process Source: WormBase
  • protein glutathionylation Source: UniProtKB
  • response to oxidative stress Source: UniProtKB
  • response to superoxide Source: WormBase
  • toxin catabolic process Source: GO_Central

Keywordsi

Molecular functionOxidoreductase, Transferase

Enzyme and pathway databases

ReactomeiR-CEL-156581 Methylation
R-CEL-156590 Glutathione conjugation
R-CEL-196836 Vitamin C (ascorbate) metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione transferase omega-1 (EC:2.5.1.181 Publication)
Alternative name(s):
Glutathione-dependent dehydroascorbate reductase (EC:1.8.5.11 Publication)
Monomethylarsonic acid reductase (EC:1.20.4.21 Publication)
Short name:
MMA(V) reductase
Gene namesi
Name:gsto-1
ORF Names:C29E4.7
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiC29E4.7 ; CE00089 ; WBGene00016204 ; gsto-1

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown results in increased sensitivity and reduced survival in response to oxidative stress inducers cumene hydroperoxide, jugalone, paraquat and arsenite, and to heat shock treatment.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi33C → A: Increased sensitivity to oxidative stress when exposed to cumene hydroperoxide. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001858891 – 250Glutathione transferase omega-1Add BLAST250

Proteomic databases

EPDiP34345
PaxDbiP34345
PeptideAtlasiP34345
PRIDEiP34345

Expressioni

Tissue specificityi

Expressed in the intestinal cells.1 Publication

Developmental stagei

Expressed in late embryos, during the L1 to L4 stages of larval development and in adult hermaphrodites.1 Publication

Gene expression databases

BgeeiWBGene00016204

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi47837, 1 interactor
DIPiDIP-24588N
IntActiP34345, 1 interactor
STRINGi6239.C29E4.7

Structurei

3D structure databases

ProteinModelPortaliP34345
SMRiP34345
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 101GST N-terminalAdd BLAST81
Domaini106 – 234GST C-terminalAdd BLAST129

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni85 – 86Glutathione bindingBy similarity2

Sequence similaritiesi

Belongs to the GST superfamily. Omega family.Curated

Phylogenomic databases

eggNOGiKOG0406 Eukaryota
ENOG410XSIX LUCA
GeneTreeiENSGT00390000005479
HOGENOMiHOG000006560
InParanoidiP34345
KOiK00799
OMAiKTRHENK
OrthoDBiEOG091G0IEA
PhylomeDBiP34345

Family and domain databases

InterProiView protein in InterPro
IPR010987 Glutathione-S-Trfase_C-like
IPR036282 Glutathione-S-Trfase_C_sf
IPR004045 Glutathione_S-Trfase_N
IPR004046 GST_C
IPR005442 GST_omega
IPR036249 Thioredoxin-like_sf
PfamiView protein in Pfam
PF00043 GST_C, 1 hit
PF02798 GST_N, 1 hit
PRINTSiPR01625 GSTRNSFRASEO
SUPFAMiSSF47616 SSF47616, 1 hit
SSF52833 SSF52833, 1 hit
PROSITEiView protein in PROSITE
PS50405 GST_CTER, 1 hit
PS50404 GST_NTER, 1 hit

Sequencei

Sequence statusi: Complete.

P34345-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLTGVTSKA IRKGDAEPPL SKGSFRVYNM RFCPWAERAM LYVAAKGIEA
60 70 80 90 100
EVVNLNVTDK LEWYWTKHYQ GKAPAVEHNG KVVIESGFIP EYLDDAFPET
110 120 130 140 150
RILPTDPYEK VQQKLLADRL TAVAHAVPLL FAVMRDRTLK DEKQRKVFEV
160 170 180 190 200
LKQAENLLAN DFYAGSQPGY PDYLSFPFFE KIWWSASLDG VVDLPTIEFP
210 220 230 240 250
GEEEYPKLTK WFQKMISSDV VQSVTQSLEH GAAFMNAYAT HQELNYDLGL
Length:250
Mass (Da):28,478
Last modified:February 1, 1994 - v1
Checksum:i4EB0E85351217C55
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080706 Genomic DNA Translation: CCD66006.1
PIRiS44768
RefSeqiNP_498728.1, NM_066327.1
UniGeneiCel.10781

Genome annotation databases

EnsemblMetazoaiC29E4.7; C29E4.7; WBGene00016204
GeneIDi183000
KEGGicel:CELE_C29E4.7

Similar proteinsi

Entry informationi

Entry nameiGSTO1_CAEEL
AccessioniPrimary (citable) accession number: P34345
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: July 18, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

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