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Protein

DNA replication regulator SLD2

Gene

SLD2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has a role in the initiation of DNA replication. Required at S-phase checkpoint. Also required for the proper activation of RAD53 in response to DNA damage and replication blocks.3 Publications

Miscellaneous

Present with 656 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • DNA replication origin binding Source: SGD
  • single-stranded DNA binding Source: SGD

GO - Biological processi

  • DNA replication initiation Source: SGD
  • DNA replication preinitiation complex assembly Source: SGD
  • DNA strand renaturation Source: SGD
  • double-strand break repair via break-induced replication Source: SGD
  • mitotic DNA replication checkpoint Source: SGD
  • mitotic DNA replication preinitiation complex assembly Source: GO_Central
  • negative regulation of protein complex assembly Source: SGD

Keywordsi

Biological processCell cycle, DNA replication

Enzyme and pathway databases

BioCyciYEAST:G3O-31894-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication regulator SLD2
Alternative name(s):
DNA replication and checkpoint protein 1
Gene namesi
Name:SLD2
Synonyms:DRC1
Ordered Locus Names:YKL108W
ORF Names:YKL463
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL108W
SGDiS000001591 SLD2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi84T → A: No interaction with DPB11. 1 Publication1
Mutagenesisi100S → A: No interaction with DPB11. 1 Publication1
Mutagenesisi208S → A: Normal interaction with DPB11. 1 Publication1
Mutagenesisi241T → A: Reduced interaction with DPB11. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000719521 – 453DNA replication regulator SLD2Add BLAST453

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei84Phosphothreonine; by CDC281 Publication1

Post-translational modificationi

Phosphorylated by CDC28 at the onset of S-phase. This phosphorylation, specifically phosphorylation of Thr-84 promotes interaction with DPB11 leading to DNA replication. Dephosphorylated by CDC14.3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP34252
PaxDbiP34252
PRIDEiP34252

PTM databases

iPTMnetiP34252

Interactioni

Subunit structurei

Interacts with DPB11.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
DPB11P470279EBI-26824,EBI-25984

Protein-protein interaction databases

BioGridi34025, 53 interactors
ComplexPortaliCPX-1188 DPB11-SLD3-SLD2 DNA replication complex
DIPiDIP-5880N
IntActiP34252, 21 interactors
MINTiP34252
STRINGi4932.YKL108W

Structurei

3D structure databases

ProteinModelPortaliP34252
SMRiP34252
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SLD2 family.Curated

Phylogenomic databases

HOGENOMiHOG000001079
InParanoidiP34252
KOiK10729
OMAiKINDPRS
OrthoDBiEOG092C3G6C

Family and domain databases

InterProiView protein in InterPro
IPR021110 DNA_rep_checkpnt_protein
PfamiView protein in Pfam
PF11719 Drc1-Sld2, 2 hits

Sequencei

Sequence statusi: Complete.

P34252-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYSFELDKLK IELKTWEHDF IDKNKREPTR DDIKSLRTVR QMYKQYSTLK
60 70 80 90 100
KKQSLQRQKV DTQESVELPA HKKDHDEVVE IGPTPQVYGK AISIFDMNLS
110 120 130 140 150
PIKPIYMTFT NNIDVNNDNS KTISNESSPR KTILLKSSPA DRTLVAEPIS
160 170 180 190 200
SVKRQLNFQM LNASSTRTPT SSPCKNRNGK LVEIKKCSPT INPPLESGKP
210 220 230 240 250
SGYYGPNSPL KLDEENIHLN ISLNSSTKRR LQIAYPSLQK TPSKDQADIS
260 270 280 290 300
TSFSPSPLIR RPLTKSLIEL AREHTEIVKE FGVLQEEDIE EEEEGEEGEN
310 320 330 340 350
GYDEKNHEDD FGLEDELIRP KVVKDIFQED DDNDDSQARE DTFIRKRPKR
360 370 380 390 400
RKVIRRLRDN DPETETAGFE RDVHKELVKL KRRKVAEFLG STSQISDTEF
410 420 430 440 450
EHDPEASSGV VSSEQKPTAK RKGRKKYNLV SNNFRRLKLP KKNRFSNGRW

GRR
Length:453
Mass (Da):52,272
Last modified:February 1, 1994 - v1
Checksum:i52B2969C97AAEAC8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X71133 Genomic DNA Translation: CAA50449.1
Z28108 Genomic DNA Translation: CAA81948.1
BK006944 Genomic DNA Translation: DAA09049.1
PIRiS37935
RefSeqiNP_012814.1, NM_001179674.1

Genome annotation databases

EnsemblFungiiYKL108W; YKL108W; YKL108W
GeneIDi853752
KEGGisce:YKL108W

Similar proteinsi

Entry informationi

Entry nameiSLD2_YEAST
AccessioniPrimary (citable) accession number: P34252
Secondary accession number(s): D6VXH9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: June 20, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

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