UniProtKB - P34233 (ASH1_YEAST)
Transcriptional regulatory protein ASH1
ASH1
Functioni
Component of the RPD3C(L) histone deacetylase complex (HDAC). Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. ASH1 is necessary to repress HO in daughter cells to block mating-type switching through its binding to HO promoter 5'-YTGAT-3' sites. Also involved in pseudohyphal growth.
4 PublicationsMiscellaneous
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 499 – 526 | GATA-type; atypicalPROSITE-ProRule annotationAdd BLAST | 28 |
GO - Molecular functioni
- cis-regulatory region sequence-specific DNA binding Source: SGD
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: SGD
- zinc ion binding Source: InterPro
GO - Biological processi
- chromatin organization Source: UniProtKB-KW
- negative regulation of G1/S transition of mitotic cell cycle by negative regulation of transcription from RNA polymerase II promoter Source: SGD
- negative regulation of transcription by RNA polymerase II Source: SGD
- positive regulation of pseudohyphal growth by positive regulation of transcription from RNA polymerase II promoter Source: SGD
Keywordsi
Molecular function | Chromatin regulator, DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Transcriptional regulatory protein ASH1Alternative name(s): Daughter cells HO repressor protein |
Gene namesi | Name:ASH1 Ordered Locus Names:YKL185W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000001668, ASH1 |
VEuPathDBi | FungiDB:YKL185W |
Subcellular locationi
Nucleus
- Nucleus 5 Publications
Note: Preferentially accumulates in daughter cell nuclei at the end of anaphase.
Nucleus
- nucleus Source: SGD
- Rpd3L complex Source: SGD
- Rpd3L-Expanded complex Source: SGD
Other locations
- cellular bud Source: SGD
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000083492 | 1 – 588 | Transcriptional regulatory protein ASH1Add BLAST | 588 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 56 | PhosphoserineCombined sources | 1 | |
Modified residuei | 465 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P34233 |
PaxDbi | P34233 |
PRIDEi | P34233 |
PTM databases
iPTMneti | P34233 |
Interactioni
Subunit structurei
Component of the RPD3C(L) complex composed of at least ASH1, CTI6, DEP1, PHO23, RPD3, RXT2, RXT3, SAP30, SDS3, SIN3, UME1 and UME6.
1 PublicationProtein-protein interaction databases
BioGRIDi | 33937, 163 interactors |
DIPi | DIP-8053N |
IntActi | P34233, 9 interactors |
MINTi | P34233 |
STRINGi | 4932.YKL185W |
Miscellaneous databases
RNActi | P34233, protein |
Structurei
3D structure databases
AlphaFoldDBi | P34233 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 85 – 109 | DisorderedSequence analysisAdd BLAST | 25 | |
Regioni | 377 – 398 | DisorderedSequence analysisAdd BLAST | 22 | |
Regioni | 417 – 495 | DisorderedSequence analysisAdd BLAST | 79 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 417 – 448 | Polar residuesSequence analysisAdd BLAST | 32 | |
Compositional biasi | 467 – 495 | Polar residuesSequence analysisAdd BLAST | 29 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 499 – 526 | GATA-type; atypicalPROSITE-ProRule annotationAdd BLAST | 28 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | ENOG502QX5C, Eukaryota |
HOGENOMi | CLU_041192_0_0_1 |
InParanoidi | P34233 |
OMAi | SDSPCWR |
Family and domain databases
CDDi | cd00202, ZnF_GATA, 1 hit |
DisProti | DP01579 |
Gene3Di | 3.30.50.10, 1 hit |
InterProi | View protein in InterPro IPR000679, Znf_GATA IPR013088, Znf_NHR/GATA |
Pfami | View protein in Pfam PF00320, GATA, 1 hit |
SMARTi | View protein in SMART SM00401, ZnF_GATA, 1 hit |
PROSITEi | View protein in PROSITE PS00344, GATA_ZN_FINGER_1, 1 hit PS50114, GATA_ZN_FINGER_2, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSSLYIKTPL HALSAGPDSH ANSSYYDNLL LPSFSNLSSN ISRNNITTDN
60 70 80 90 100
NINSASPRKY SFHSLNVSPI LSPISLANEI LGKKSNTAPA SPHHMDYNPI
110 120 130 140 150
SSLTPGNSPE FNKASLSQIS FTNPLNYGSG LGFSSNSQPR LPLLDRLSSV
160 170 180 190 200
SLSKRPERPQ QSLPSLRHLQ LLPSPLLQEN AARFPDTSKR TSNWKTDLTH
210 220 230 240 250
WCKDTNYQDY VKIREEVAHF KPLSIPNLTN NQNNDSFNYG KELESTRSSK
260 270 280 290 300
FHSPSKESFD RTKLIPSILE AKDQFKDLSN NAWSITPPVT PPMSPPTNRT
310 320 330 340 350
MERTTLRGVE ASFFEGKSSN NDSIFNPIIS EKLVQEVKHQ RQLRGNSFPM
360 370 380 390 400
PNASHKKTNS FKALQIKKLL ANRDILSNNS KSNVRKPSKN KISKQASNVF
410 420 430 440 450
GNTARQLVMK LDNASYSSVS ASSSPSPSTP TKSGKMRSRS SSPVRPKAYT
460 470 480 490 500
PSPRSPNYHR FALDSPPQSP RRSSNSSITK KGSRRSSGSS PTRHTTRVCV
510 520 530 540 550
SCHSSDSPCW RPSWSPRKQD QLCNSCGLRY KKTHTRCLND LCRKIPTKGE
560 570 580
INIMKSNGID KEFVPERNCE IEGYRCLFCN YITETVEN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X74151 Genomic DNA Translation: CAA52253.1 Z28185 Genomic DNA Translation: CAA82028.1 M88605 Genomic DNA Translation: AAA34830.1 BK006944 Genomic DNA Translation: DAA08981.1 |
PIRi | S34685 |
RefSeqi | NP_012736.1, NM_001179751.1 |
Genome annotation databases
EnsemblFungii | YKL185W_mRNA; YKL185W; YKL185W |
GeneIDi | 853650 |
KEGGi | sce:YKL185W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X74151 Genomic DNA Translation: CAA52253.1 Z28185 Genomic DNA Translation: CAA82028.1 M88605 Genomic DNA Translation: AAA34830.1 BK006944 Genomic DNA Translation: DAA08981.1 |
PIRi | S34685 |
RefSeqi | NP_012736.1, NM_001179751.1 |
3D structure databases
AlphaFoldDBi | P34233 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 33937, 163 interactors |
DIPi | DIP-8053N |
IntActi | P34233, 9 interactors |
MINTi | P34233 |
STRINGi | 4932.YKL185W |
PTM databases
iPTMneti | P34233 |
Proteomic databases
MaxQBi | P34233 |
PaxDbi | P34233 |
PRIDEi | P34233 |
Genome annotation databases
EnsemblFungii | YKL185W_mRNA; YKL185W; YKL185W |
GeneIDi | 853650 |
KEGGi | sce:YKL185W |
Organism-specific databases
SGDi | S000001668, ASH1 |
VEuPathDBi | FungiDB:YKL185W |
Phylogenomic databases
eggNOGi | ENOG502QX5C, Eukaryota |
HOGENOMi | CLU_041192_0_0_1 |
InParanoidi | P34233 |
OMAi | SDSPCWR |
Miscellaneous databases
PROi | PR:P34233 |
RNActi | P34233, protein |
Family and domain databases
CDDi | cd00202, ZnF_GATA, 1 hit |
DisProti | DP01579 |
Gene3Di | 3.30.50.10, 1 hit |
InterProi | View protein in InterPro IPR000679, Znf_GATA IPR013088, Znf_NHR/GATA |
Pfami | View protein in Pfam PF00320, GATA, 1 hit |
SMARTi | View protein in SMART SM00401, ZnF_GATA, 1 hit |
PROSITEi | View protein in PROSITE PS00344, GATA_ZN_FINGER_1, 1 hit PS50114, GATA_ZN_FINGER_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ASH1_YEAST | |
Accessioni | P34233Primary (citable) accession number: P34233 Secondary accession number(s): D6VX15 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | February 1, 1994 | |
Last modified: | May 25, 2022 | |
This is version 176 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XI
Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names