Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Deoxyribonuclease Tat-D

Gene

YBL055C

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has both endo- and exonuclease activities. Incises double-stranded DNA without obvious specificity via its endonuclease activity and excises the DNA from the 3'-to 5'-end by its exonuclease activity. May have a role in apoptosis.1 Publication

Miscellaneous

Present with 1460 molecules/cell in log phase SD medium.1 Publication

Cofactori

Mg2+1 PublicationNote: Divalent metal cations. Has optimal activity with Mg2+.1 Publication

pH dependencei

Optimum pH is 5.1 Publication

Temperature dependencei

Optimum temperature is 30 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi185Divalent metal cation 1By similarity1
Metal bindingi185Divalent metal cation 2By similarity1
Metal bindingi226Divalent metal cation 2By similarity1
Metal bindingi277Divalent metal cation 2By similarity1
Metal bindingi327Divalent metal cation 1By similarity1

GO - Molecular functioni

  • 3'-5'-exodeoxyribonuclease activity Source: SGD
  • endodeoxyribonuclease activity, producing 5'-phosphomonoesters Source: InterPro
  • endonuclease activity Source: SGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • apoptotic DNA fragmentation Source: SGD
  • cellular response to oxidative stress Source: SGD

Keywordsi

Molecular functionHydrolase, Nuclease
LigandMetal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-28953-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyribonuclease Tat-D (EC:3.1.21.-)
Gene namesi
Ordered Locus Names:YBL055C
ORF Names:YBL0511, YBL0512
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBL055C
SGDiS000000151 YBL055C

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi185E → A: Reduces enzymatic activities by 50%. 1 Publication1
Mutagenesisi325E → A: Reduces enzymatic activities by 50%. 1 Publication1
Mutagenesisi327D → A: Reduces enzymatic activities by almost 95%. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002019941 – 418Deoxyribonuclease Tat-DAdd BLAST418

Proteomic databases

MaxQBiP34220
PaxDbiP34220
PRIDEiP34220
TopDownProteomicsiP34220

Interactioni

Protein-protein interaction databases

BioGridi32644, 66 interactors
MINTiP34220
STRINGi4932.YBL055C

Structurei

3D structure databases

ProteinModelPortaliP34220
SMRiP34220
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00720000108776
HOGENOMiHOG000201523
KOiK03424
OMAiETDGPWC
OrthoDBiEOG092C3EIV

Family and domain databases

CDDicd01310 TatD_DNAse, 1 hit
InterProiView protein in InterPro
IPR018228 DNase_TatD-rel_CS
IPR032466 Metal_Hydrolase
IPR001130 TatD_family
PANTHERiPTHR10060 PTHR10060, 1 hit
PfamiView protein in Pfam
PF01026 TatD_DNase, 1 hit
SUPFAMiSSF51556 SSF51556, 4 hits
PROSITEiView protein in PROSITE
PS01090 TATD_2, 1 hit
PS01091 TATD_3, 1 hit

Sequencei

Sequence statusi: Complete.

P34220-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWGILLKSSN KSCSRLWKPI LTQYYSMTST ATDSPLKYYD IGLNLTDPMF
60 70 80 90 100
HGIYNGKQYH PADYVKLLER AAQRHVKNAL VTGSSIAESQ SAIELVSSVK
110 120 130 140 150
DLSPLKLYHT IGVHPCCVNE FADASQGDKA SASIDNPSMD EAYNESLYAK
160 170 180 190 200
VISNPSFAQG KLKELYDLMN QQAKPHDTSF RSIGEIGLDY DRFHYSSKEM
210 220 230 240 250
QKVFFEEQLK ISCLNDKLSS YPLFLHMRSA CDDFVQILER FIAGFTDERD
260 270 280 290 300
TFQLQKLGAS SSSGFYKFHP DRKLVVHSFT GSAIDLQKLL NLSPNIFIGV
310 320 330 340 350
NGCSLRTEEN LAVVKQIPTE RLLLETDAPW CEIKRTHASF QYLAKYQEVR
360 370 380 390 400
DFEYPAFKSV KKNKLADKLN AEELYMVKGR NEPCNMEQVA IVVSEVKDVD
410
LATLIDTTWK TTCKIFGE
Length:418
Mass (Da):47,390
Last modified:February 1, 1994 - v1
Checksum:iF1107C1B8E94CAD1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti278S → P in AAT92645 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z23261 Genomic DNA Translation: CAA80792.1
Z35816 Genomic DNA Translation: CAA84875.1
AY692626 Genomic DNA Translation: AAT92645.1
BK006936 Genomic DNA Translation: DAA07065.1
PIRiS39833
RefSeqiNP_009498.1, NM_001178295.1

Genome annotation databases

EnsemblFungiiYBL055C; YBL055C; YBL055C
GeneIDi852225
KEGGisce:YBL055C

Similar proteinsi

Entry informationi

Entry nameiYBF5_YEAST
AccessioniPrimary (citable) accession number: P34220
Secondary accession number(s): D6VPU5, Q6B2V4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: June 20, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health