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UniProtKB - P34217 (PIN4_YEAST)
Protein
RNA-binding protein PIN4
Gene
PIN4
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Involved in normal G2/M phase transition of the mitotic cell cycle. In association with RAD53, also involved in checkpoint control in response to DNA damage.
1 PublicationMiscellaneous
Present with 4630 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- mRNA binding Source: SGD
GO - Biological processi
- DNA damage checkpoint signaling Source: SGD
- G2/M transition of mitotic cell cycle Source: SGD
- nuclear mRNA surveillance Source: GO_Central
- poly(A)+ mRNA export from nucleus Source: GO_Central
- regulation of mRNA stability involved in response to oxidative stress Source: GO_Central
Keywordsi
Molecular function | RNA-binding |
Names & Taxonomyi
Protein namesi | Recommended name: RNA-binding protein PIN4Alternative name(s): Psi inducibility protein 4 |
Gene namesi | Name:PIN4 Synonyms:MDT1 Ordered Locus Names:YBL051C ORF Names:YBL0506, YBL0516 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000000147, PIN4 |
VEuPathDBi | FungiDB:YBL051C |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Nucleus
- nucleus Source: GO_Central
- post-mRNA release spliceosomal complex Source: GO_Central
Other locations
- cytoplasm Source: SGD
- ribonucleoprotein complex Source: GO_Central
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 305 | T → A: No interaction with RAD53. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000082028 | 1 – 668 | RNA-binding protein PIN4Add BLAST | 668 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 56 | PhosphoserineCombined sources | 1 | |
Modified residuei | 189 | PhosphoserineCombined sources | 1 | |
Modified residuei | 191 | PhosphoserineCombined sources | 1 | |
Modified residuei | 194 | PhosphoserineCombined sources | 1 | |
Modified residuei | 197 | PhosphoserineCombined sources | 1 | |
Modified residuei | 305 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 393 | PhosphoserineCombined sources | 1 | |
Modified residuei | 466 | PhosphoserineCombined sources | 1 | |
Modified residuei | 541 | PhosphoserineCombined sources | 1 | |
Modified residuei | 636 | PhosphoserineCombined sources | 1 | |
Modified residuei | 638 | PhosphoserineCombined sources | 1 | |
Modified residuei | 640 | PhosphoserineCombined sources | 1 | |
Modified residuei | 653 | PhosphoserineCombined sources | 1 | |
Modified residuei | 655 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Hyperphosphorylated in response to DNA damage by MEC1.1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P34217 |
PaxDbi | P34217 |
PRIDEi | P34217 |
PTM databases
iPTMneti | P34217 |
Interactioni
Subunit structurei
Interacts with RAD53.
1 PublicationBinary interactionsi
P34217
With | #Exp. | IntAct |
---|---|---|
RAD53 [P22216] | 3 | EBI-21256,EBI-17843 |
Protein-protein interaction databases
BioGRIDi | 32647, 323 interactors |
DIPi | DIP-1905N |
ELMi | P34217 |
IntActi | P34217, 44 interactors |
MINTi | P34217 |
STRINGi | 4932.YBL051C |
Miscellaneous databases
RNActi | P34217, protein |
Structurei
3D structure databases
SMRi | P34217 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P34217 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 85 – 163 | RRMPROSITE-ProRule annotationAdd BLAST | 79 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 77 | DisorderedSequence analysisAdd BLAST | 77 | |
Regioni | 168 – 214 | DisorderedSequence analysisAdd BLAST | 47 | |
Regioni | 374 – 398 | DisorderedSequence analysisAdd BLAST | 25 | |
Regioni | 420 – 570 | DisorderedSequence analysisAdd BLAST | 151 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 36 | Polar residuesSequence analysisAdd BLAST | 36 | |
Compositional biasi | 48 – 75 | Polar residuesSequence analysisAdd BLAST | 28 | |
Compositional biasi | 168 – 186 | Basic and acidic residuesSequence analysisAdd BLAST | 19 | |
Compositional biasi | 187 – 214 | Polar residuesSequence analysisAdd BLAST | 28 |
Phylogenomic databases
eggNOGi | KOG0108, Eukaryota |
HOGENOMi | CLU_029387_0_0_1 |
OMAi | KMLPQAE |
Family and domain databases
CDDi | cd02639, R3H_RRM, 1 hit cd12253, RRM_PIN4_like, 1 hit |
Gene3Di | 3.30.70.330, 1 hit |
IDEALi | IID50186 |
InterProi | View protein in InterPro IPR012677, Nucleotide-bd_a/b_plait_sf IPR034186, PIN4-like_RRM IPR034069, R3H_Cip2 IPR035979, RBD_domain_sf IPR000504, RRM_dom |
Pfami | View protein in Pfam PF00076, RRM_1, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 1 hit |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 1 hit |
i Sequence
Sequence statusi: Complete.
P34217-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
METSSFENAP PAAINDAQDN NINTETNDQE TNQQSIETRD AIDKENGVQT
60 70 80 90 100
ETGENSAKNA EQNVSSTNLN NAPTNGALDD DVIPNAIVIK NIPFAIKKEQ
110 120 130 140 150
LLDIIEEMDL PLPYAFNYHF DNGIFRGLAF ANFTTPEETT QVITSLNGKE
160 170 180 190 200
ISGRKLKVEY KKMLPQAERE RIEREKREKR GQLEEQHRSS SNLSLDSLSK
210 220 230 240 250
MSGSGNNNTS NNQLFSTLMN GINANSMMNS PMNNTINNNS SNNNNSGNII
260 270 280 290 300
LNQPSLSAQH TSSSLYQTNV NNQAQMSTER FYAPLPSTST LPLPPQQLDF
310 320 330 340 350
NDPDTLEIYS QLLLFKDREK YYYELAYPMG ISASHKRIIN VLCSYLGLVE
360 370 380 390 400
VYDPRFIIIR RKILDHANLQ SHLQQQGQMT SAHPLQPNST GGSMNRSQSY
410 420 430 440 450
TSLLQAHAAA AANSISNQAV NNSSNSNTIN SNNGNGNNVI INNNSASSTP
460 470 480 490 500
KISSQGQFSM QPTLTSPKMN IHHSSQYNSA DQPQQPQPQT QQNVQSAAQQ
510 520 530 540 550
QQSFLRQQAT LTPSSRIPSG YSANHYQINS VNPLLRNSQI SPPNSQIPIN
560 570 580 590 600
SQTLSQAQPP AQSQTQQRVP VAYQNASLSS QQLYNLNGPS SANSQSQLLP
610 620 630 640 650
QHTNGSVHSN FSYQSYHDES MLSAHNLNSA DLIYKSLSHS GLDDGLEQGL
660
NRSLSGLDLQ NQNKKNLW
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z23261 Genomic DNA Translation: CAA80795.1 Z35812 Genomic DNA Translation: CAA84871.1 BK006936 Genomic DNA Translation: DAA07068.1 |
PIRi | S39836 |
RefSeqi | NP_009502.1, NM_001178291.1 |
Genome annotation databases
EnsemblFungii | YBL051C_mRNA; YBL051C; YBL051C |
GeneIDi | 852229 |
KEGGi | sce:YBL051C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z23261 Genomic DNA Translation: CAA80795.1 Z35812 Genomic DNA Translation: CAA84871.1 BK006936 Genomic DNA Translation: DAA07068.1 |
PIRi | S39836 |
RefSeqi | NP_009502.1, NM_001178291.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2A0T | NMR | - | B | 301-310 | [»] | |
SMRi | P34217 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 32647, 323 interactors |
DIPi | DIP-1905N |
ELMi | P34217 |
IntActi | P34217, 44 interactors |
MINTi | P34217 |
STRINGi | 4932.YBL051C |
PTM databases
iPTMneti | P34217 |
Proteomic databases
MaxQBi | P34217 |
PaxDbi | P34217 |
PRIDEi | P34217 |
Genome annotation databases
EnsemblFungii | YBL051C_mRNA; YBL051C; YBL051C |
GeneIDi | 852229 |
KEGGi | sce:YBL051C |
Organism-specific databases
SGDi | S000000147, PIN4 |
VEuPathDBi | FungiDB:YBL051C |
Phylogenomic databases
eggNOGi | KOG0108, Eukaryota |
HOGENOMi | CLU_029387_0_0_1 |
OMAi | KMLPQAE |
Miscellaneous databases
EvolutionaryTracei | P34217 |
PROi | PR:P34217 |
RNActi | P34217, protein |
Family and domain databases
CDDi | cd02639, R3H_RRM, 1 hit cd12253, RRM_PIN4_like, 1 hit |
Gene3Di | 3.30.70.330, 1 hit |
IDEALi | IID50186 |
InterProi | View protein in InterPro IPR012677, Nucleotide-bd_a/b_plait_sf IPR034186, PIN4-like_RRM IPR034069, R3H_Cip2 IPR035979, RBD_domain_sf IPR000504, RRM_dom |
Pfami | View protein in Pfam PF00076, RRM_1, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 1 hit |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PIN4_YEAST | |
Accessioni | P34217Primary (citable) accession number: P34217 Secondary accession number(s): D6VPU8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | February 1, 1994 | |
Last modified: | September 29, 2021 | |
This is version 171 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome II
Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references