UniProtKB - P34160 (NCBP1_YEAST)
Nuclear cap-binding protein complex subunit 1
STO1
Functioni
Component of the CBC complex, which binds co-transcriptionally to the 5'-cap of pre-mRNAs and is involved in maturation, export and degradation of nuclear mRNAs. The CBC complex is required for efficient pre-mRNA splicing through efficient commitment complex and spliceosome formation. Together with NPL3, the CBC complex is required for export of mRNAs out of the nucleus. The CBC complex is also involved in nuclear mRNA degradation, probably by directing the mRNAs to the sites of degradation. Affects replication of the positive-strand RNA virus BMV.
13 PublicationsMiscellaneous
GO - Molecular functioni
- mRNA binding Source: SGD
- RNA cap binding Source: GO_Central
GO - Biological processi
- 7-methylguanosine mRNA capping Source: UniProtKB-KW
- mRNA 3'-end processing Source: ComplexPortal
- mRNA export from nucleus Source: ComplexPortal
- mRNA splicing, via spliceosome Source: ComplexPortal
- mRNA transcription by RNA polymerase II Source: ComplexPortal
- nuclear-transcribed mRNA catabolic process Source: ComplexPortal
- nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: SGD
- primary miRNA processing Source: ComplexPortal
- response to osmotic stress Source: ComplexPortal
Keywordsi
Molecular function | RNA-binding |
Biological process | mRNA capping, mRNA processing, mRNA splicing, mRNA transport, Nonsense-mediated mRNA decay, Transport |
Enzyme and pathway databases
Reactomei | R-SCE-113418, Formation of the Early Elongation Complex R-SCE-674695, RNA Polymerase II Pre-transcription Events R-SCE-72086, mRNA Capping R-SCE-77595, Processing of Intronless Pre-mRNAs R-SCE-975956, Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) R-SCE-975957, Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) |
Names & Taxonomyi
Protein namesi | Recommended name: Nuclear cap-binding protein complex subunit 1Alternative name(s): 80 kDa nuclear cap-binding protein Short name: CBP80 Short name: NCBP 80 kDa subunit Glycolysis regulation protein 3 Protein SUT1 Suppressor of TOP1 protein |
Gene namesi | Name:STO1 Synonyms:CBC1, CBP80, GCR3, SUT1 Ordered Locus Names:YMR125W ORF Names:YM8564.07, YM9553.01 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000004732, STO1 |
VEuPathDBi | FungiDB:YMR125W |
Subcellular locationi
Nucleus
- Nucleus 3 Publications
Other locations
- perinuclear region 1 Publication
Note: Predominantly nuclear, is able to exit the nucleus in an RNA-dependent manner.1 Publication
Nucleus
- commitment complex Source: SGD
- nucleus Source: ComplexPortal
Other locations
- mRNA cap binding complex Source: ComplexPortal
- nuclear cap binding complex Source: SGD
- perinuclear region of cytoplasm Source: UniProtKB-SubCell
- polysome Source: SGD
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000087447 | 1 – 861 | Nuclear cap-binding protein complex subunit 1Add BLAST | 861 |
Proteomic databases
MaxQBi | P34160 |
PaxDbi | P34160 |
PRIDEi | P34160 |
PTM databases
iPTMneti | P34160 |
Interactioni
Subunit structurei
Component of the nuclear cap-binding complex (CBC), a heterodimer composed of STO1/CBC1 and CBC2 that interacts with capped RNAs. The complex interacts strongly with the importin subunit alpha SRP1. The SRP1-CBC trimer also binds to capped RNAs, but formation of the importin alpha/beta heterodimer upon binding of KAP95 to SRP1 in the cytoplasm causes dissociation of CBC from the RNA. The CBC complex is part of the commitment complex 1 (CC1), binding to the cap of pre-mRNA and interacting with U1 snRNP subunits MUD2 and SNU56. The CBC complex is part of the NRD1 complex, composed of CBC2, NAB1, NRD1, SEN1 and STO1/CBC2. The CBC complex also interacts with NPL3 and eIF4G (TIF4631 and TIF4632).
8 PublicationsBinary interactionsi
P34160
With | #Exp. | IntAct |
---|---|---|
CBC2 [Q08920] | 5 | EBI-745,EBI-33556 |
Protein-protein interaction databases
BioGRIDi | 35302, 200 interactors |
ComplexPortali | CPX-1657, Nuclear cap-binding complex |
DIPi | DIP-4434N |
IntActi | P34160, 52 interactors |
MINTi | P34160 |
STRINGi | 4932.YMR125W |
Miscellaneous databases
RNActi | P34160, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 36 – 264 | MIF4GAdd BLAST | 229 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 22 – 30 | Nuclear localization signalSequence analysis | 9 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1104, Eukaryota |
GeneTreei | ENSGT00390000001733 |
HOGENOMi | CLU_011380_0_0_1 |
InParanoidi | P34160 |
OMAi | QPFKIPF |
Family and domain databases
IDEALi | IID50172 |
InterProi | View protein in InterPro IPR016024, ARM-type_fold IPR027159, CBP80 IPR015172, MIF4G-like_typ-1 IPR015174, MIF4G-like_typ-2 IPR003890, MIF4G-like_typ-3 |
PANTHERi | PTHR12412, PTHR12412, 1 hit |
Pfami | View protein in Pfam PF02854, MIF4G, 1 hit PF09088, MIF4G_like, 1 hit PF09090, MIF4G_like_2, 1 hit |
SMARTi | View protein in SMART SM00543, MIF4G, 1 hit |
SUPFAMi | SSF48371, SSF48371, 3 hits |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MFNRKRRGDF DEDENYRDFR PRMPKRQRIP PVVQLCKEMM PDIRTIGESV
60 70 80 90 100
KAFEDDIKFL SEAIMNEYGH EDYFNNALLS TLNAVVVEQP QKQAAIALLT
110 120 130 140 150
MVVNSKNNVA GKSIINYFFE ELQKWCKQTY NDEFKSTSNE TGPWNKIKLI
160 170 180 190 200
LRFLSILSPM FLVDELINIY KSLFELSIEL NNLDPGNRVP LSEAIYTNTL
210 220 230 240 250
LNIPYLFFFN RNNDGLRTKV EELLAYVEQN YLVKTTDINL LREYNGEPPY
260 270 280 290 300
EMVELVRVVL PNVKKALINN LEQLNELFPD WNHLLTPQTG DEGFNDALTL
310 320 330 340 350
PSVDDLKSFV RLNKNFGSVD SMWKTPRYAF HVYLPNSAGN FETVVPISTY
360 370 380 390 400
AGQLFNDIII DLVESLEFNR KEVARQVITL DLFFKAGIFT EPGESIAQLI
410 420 430 440 450
ATYEENPLAP TFKIEDLAIE TILGLIFKLP SVSQPFAYFY TLLVDICQNS
460 470 480 490 500
PKAIAPVFGR AFRFFYSHLD SLDFELKLRY LDWFSIQMSN FNFSWKWNEW
510 520 530 540 550
EDDSIKFGKY FYNPKVNFAK NLIQKELRLT SNFSEVEDSL PQEFTKYLDT
560 570 580 590 600
SYIPRDQLIN YYQSLFTGYT VEEDSVRKND LYFRQEGVPM ENTVRKILDY
610 620 630 640 650
THKANNSREV TELESILGEL KNEYGSIISD FNRFVIILLV QAVTDSGSRS
660 670 680 690 700
LSHANKYIND LKEDLKTIFA KIELDIETKE YIIIEAVLTF WNANPQTGFL
710 720 730 740 750
VADAFKYAGL LTSRTIFTFI FNETGLKNNG LIEATAIEAV FRNLSQQISE
760 770 780 790 800
ENESGNNFEF VFERLCTIAN STIDLLDVNA DEDIEIPKVN GEMDIDDIED
810 820 830 840 850
DKLDLKWKYF TVIGFIKSIL RRYSHEYREL ADKFIANIDN AIPHESTRRT
860
ISNWIQETKE V
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 164 | D → V in L27744 (PubMed:10733586).Curated | 1 | |
Sequence conflicti | 633 | R → I in L27744 (PubMed:10733586).Curated | 1 | |
Sequence conflicti | 704 | A → R in L27744 (PubMed:10733586).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D10224 Genomic DNA Translation: BAA01076.1 Sequence problems. L07650 Genomic DNA No translation available. L27744 Genomic DNA No translation available. Z49273 Genomic DNA Translation: CAA89274.1 Z48622 Genomic DNA Translation: CAA88550.1 BK006946 Genomic DNA Translation: DAA10022.1 |
PIRi | A44919 |
RefSeqi | NP_013844.2, NM_001182626.1 |
Genome annotation databases
EnsemblFungii | YMR125W_mRNA; YMR125W; YMR125W |
GeneIDi | 855155 |
KEGGi | sce:YMR125W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D10224 Genomic DNA Translation: BAA01076.1 Sequence problems. L07650 Genomic DNA No translation available. L27744 Genomic DNA No translation available. Z49273 Genomic DNA Translation: CAA89274.1 Z48622 Genomic DNA Translation: CAA88550.1 BK006946 Genomic DNA Translation: DAA10022.1 |
PIRi | A44919 |
RefSeqi | NP_013844.2, NM_001182626.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3UKY | X-ray | 2.35 | C | 1-30 | [»] | |
6N7P | electron microscopy | 3.60 | X | 1-861 | [»] | |
AlphaFoldDBi | P34160 | |||||
SMRi | P34160 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 35302, 200 interactors |
ComplexPortali | CPX-1657, Nuclear cap-binding complex |
DIPi | DIP-4434N |
IntActi | P34160, 52 interactors |
MINTi | P34160 |
STRINGi | 4932.YMR125W |
PTM databases
iPTMneti | P34160 |
Proteomic databases
MaxQBi | P34160 |
PaxDbi | P34160 |
PRIDEi | P34160 |
Genome annotation databases
EnsemblFungii | YMR125W_mRNA; YMR125W; YMR125W |
GeneIDi | 855155 |
KEGGi | sce:YMR125W |
Organism-specific databases
SGDi | S000004732, STO1 |
VEuPathDBi | FungiDB:YMR125W |
Phylogenomic databases
eggNOGi | KOG1104, Eukaryota |
GeneTreei | ENSGT00390000001733 |
HOGENOMi | CLU_011380_0_0_1 |
InParanoidi | P34160 |
OMAi | QPFKIPF |
Enzyme and pathway databases
Reactomei | R-SCE-113418, Formation of the Early Elongation Complex R-SCE-674695, RNA Polymerase II Pre-transcription Events R-SCE-72086, mRNA Capping R-SCE-77595, Processing of Intronless Pre-mRNAs R-SCE-975956, Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) R-SCE-975957, Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) |
Miscellaneous databases
PROi | PR:P34160 |
RNActi | P34160, protein |
Family and domain databases
IDEALi | IID50172 |
InterProi | View protein in InterPro IPR016024, ARM-type_fold IPR027159, CBP80 IPR015172, MIF4G-like_typ-1 IPR015174, MIF4G-like_typ-2 IPR003890, MIF4G-like_typ-3 |
PANTHERi | PTHR12412, PTHR12412, 1 hit |
Pfami | View protein in Pfam PF02854, MIF4G, 1 hit PF09088, MIF4G_like, 1 hit PF09090, MIF4G_like_2, 1 hit |
SMARTi | View protein in SMART SM00543, MIF4G, 1 hit |
SUPFAMi | SSF48371, SSF48371, 3 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | NCBP1_YEAST | |
Accessioni | P34160Primary (citable) accession number: P34160 Secondary accession number(s): D6VZU8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | October 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 196 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XIII
Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families