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Entry version 180 (26 Feb 2020)
Sequence version 2 (01 Nov 1995)
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Protein

Nucleoporin NIC96

Gene

NIC96

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. NIC96, which is localized to the core of the NPC and the distal ring of the nuclear basket, is required for de novo assembly of NPCs. It is involved in nuclear GSP1 import.11 Publications

Miscellaneous

Present with 15500 molecules/cell in log phase SD medium.1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

  • structural constituent of nuclear pore Source: SGD

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processmRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
YEAST:G3O-30455-MONOMER

Protein family/group databases

Transport Classification Database

More...
TCDBi
1.I.1.1.1 the eukaryotic nuclear pore complex (e-npc) family

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Nucleoporin NIC96
Alternative name(s):
96 kDa nucleoporin-interacting component
Nuclear pore protein NIC96
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:NIC96
Ordered Locus Names:YFR002W
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri559292 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002311 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome VI

Organism-specific databases

Eukaryotic Pathogen Database Resources

More...
EuPathDBi
FungiDB:YFR002W

Saccharomyces Genome Database

More...
SGDi
S000001898 NIC96

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Nuclear pore complex, Nucleus

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001247871 – 839Nucleoporin NIC96Add BLAST839

Proteomic databases

MaxQB - The MaxQuant DataBase

More...
MaxQBi
P34077

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P34077

PRoteomics IDEntifications database

More...
PRIDEi
P34077

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
P34077

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

The nuclear pore complex (NPC) constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active, nuclear transport receptor-mediated bidirectional transport of macromolecules such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal subunits across the nuclear envelope. The 55-60 MDa NPC is composed of at least 31 different subunits: ASM4, CDC31, GLE1, GLE2, NDC1, NIC96, NSP1, NUP1, NUP2, NUP100, NUP116, NUP120, NUP133, NUP145, NUP157, NUP159, NUP170, NUP188, NUP192, NUP42, NUP49, NUP53, NUP57, NUP60, NUP82, NUP84, NUP85, POM152, POM34, SEC13 and SEH1. Due to its 8-fold rotational symmetry, all subunits are present with 8 copies or multiples thereof. NIC96 is part of three NPC subcomplexes, interacting with NSP1 of the NUP57 subcomplex (NIC96, NSP1, NUP49, NUP57), with NUP120 of the NUP84 subcomplex (SEH1,NUP85, NUP120, NUP145C, SEC13, NUP84, NUP133), and with NUP53 of the NUP53-NUP59-NUP170 subcomplex. The interaction with NUP53 is cell cycle dependent. NIC96 is also associated with the distal ring of the nuclear basket and interacts here with MLP2, which forms together with MLP1 nuclear pore-attached intranuclear filaments.

8 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Show more details

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
31150, 85 interactors

ComplexPortal: manually curated resource of macromolecular complexes

More...
ComplexPortali
CPX-824 Nuclear pore complex

Database of interacting proteins

More...
DIPi
DIP-745N

Protein interaction database and analysis system

More...
IntActi
P34077, 31 interactors

Molecular INTeraction database

More...
MINTi
P34077

STRING: functional protein association networks

More...
STRINGi
4932.YFR002W

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...
RNActi
P34077 protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1839
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

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SMRi
P34077

Database of comparative protein structure models

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ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

More...
PDBe-KBi
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...
EvolutionaryTracei
P34077

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni25 – 60Leucine zipper-like heptad repeat, required for interaction with NSP1Add BLAST36

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi187 – 236Asn-richAdd BLAST50

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_011846_0_0_1

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P34077

KEGG Orthology (KO)

More...
KOi
K14309

Identification of Orthologs from Complete Genome Data

More...
OMAi
RMPGDTN

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR007231 Nucleoporin_int_Nup93/Nic96

The PANTHER Classification System

More...
PANTHERi
PTHR11225 PTHR11225, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF04097 Nic96, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P34077-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLETLRGNKL HSGTSKGANK KLNELLESSD NLPSASSELG SIQVSINELR
60 70 80 90 100
RRVFQLRSKN KASKDYTKAH YLLANSGLSF EDVDAFIKDL QTNQFLEPNP
110 120 130 140 150
PKIIESEELE FYIRTKKEEN ILMSIEQLLN GATKDFDNFI NHNLNLDWAQ
160 170 180 190 200
HKNEVMKNFG ILIQDKKTVD HKKSISSLDP KLPSWGNKGN NILNSNESRL
210 220 230 240 250
NVNENNILRE KFENYARIVF QFNNSRQANG NFDIANEFIS ILSSANGTRN
260 270 280 290 300
AQLLESWKIL ESMKSKDINI VEVGKQYLEQ QFLQYTDNLY KKNMNEGLAT
310 320 330 340 350
NVNKIKSFID TKLKKADKSW KISNLTVING VPIWALIFYL LRAGLIKEAL
360 370 380 390 400
QVLVENKANI KKVEQSFLTY FKAYASSKDH GLPVEYSTKL HTEYNQHIKS
410 420 430 440 450
SLDGDPYRLA VYKLIGRCDL SRKNIPAVTL SIEDWLWMHL MLIKEKDAEN
460 470 480 490 500
DPVYERYSLE DFQNIIISYG PSRFSNYYLQ TLLLSGLYGL AIDYTYTFSE
510 520 530 540 550
MDAVHLAIGL ASLKLFKIDS STRLTKKPKR DIRFANILAN YTKSFRYSDP
560 570 580 590 600
RVAVEYLVLI TLNEGPTDVE LCHEALRELV LETKEFTVLL GKIGRDGARI
610 620 630 640 650
PGVIEERQPL LHVRDEKEFL HTITEQAARR ADEDGRIYDS ILLYQLAEEY
660 670 680 690 700
DIVITLVNSL LSDTLSASDL DQPLVGPDDN SETNPVLLAR RMASIYFDNA
710 720 730 740 750
GISRQIHVKN KEICMLLLNI SSIRELYFNK QWQETLSQME LLDLLPFSDE
760 770 780 790 800
LSARKKAQDF SNLDDNIVKN IPNLLIITLS CISNMIHILN ESKYQSSTKG
810 820 830
QQIDSLKNVA RQCMIYAGMI QYRMPRETYS TLINIDVSL
Length:839
Mass (Da):96,174
Last modified:November 1, 1995 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i48EC3BC71281CB44
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti59Missing in CAA51427 (PubMed:7688296).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
X72923 Genomic DNA Translation: CAA51427.1
D50617 Genomic DNA Translation: BAA09241.1
BK006940 Genomic DNA Translation: DAA12442.1

Protein sequence database of the Protein Information Resource

More...
PIRi
S35319

NCBI Reference Sequences

More...
RefSeqi
NP_116657.1, NM_001179967.1

Genome annotation databases

Ensembl fungal genome annotation project

More...
EnsemblFungii
YFR002W_mRNA; YFR002W; YFR002W

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
850552

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
sce:YFR002W

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72923 Genomic DNA Translation: CAA51427.1
D50617 Genomic DNA Translation: BAA09241.1
BK006940 Genomic DNA Translation: DAA12442.1
PIRiS35319
RefSeqiNP_116657.1, NM_001179967.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2QX5X-ray2.50A/B186-839[»]
2RFOX-ray2.60A/B189-839[»]
SMRiP34077
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGridi31150, 85 interactors
ComplexPortaliCPX-824 Nuclear pore complex
DIPiDIP-745N
IntActiP34077, 31 interactors
MINTiP34077
STRINGi4932.YFR002W

Protein family/group databases

TCDBi1.I.1.1.1 the eukaryotic nuclear pore complex (e-npc) family

PTM databases

iPTMnetiP34077

Proteomic databases

MaxQBiP34077
PaxDbiP34077
PRIDEiP34077

Genome annotation databases

EnsemblFungiiYFR002W_mRNA; YFR002W; YFR002W
GeneIDi850552
KEGGisce:YFR002W

Organism-specific databases

EuPathDBiFungiDB:YFR002W
SGDiS000001898 NIC96

Phylogenomic databases

HOGENOMiCLU_011846_0_0_1
InParanoidiP34077
KOiK14309
OMAiRMPGDTN

Enzyme and pathway databases

BioCyciYEAST:G3O-30455-MONOMER

Miscellaneous databases

EvolutionaryTraceiP34077

Protein Ontology

More...
PROi
PR:P34077
RNActiP34077 protein

Family and domain databases

InterProiView protein in InterPro
IPR007231 Nucleoporin_int_Nup93/Nic96
PANTHERiPTHR11225 PTHR11225, 1 hit
PfamiView protein in Pfam
PF04097 Nic96, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiNIC96_YEAST
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P34077
Secondary accession number(s): D6VTN2
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: November 1, 1995
Last modified: February 26, 2020
This is version 180 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
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