UniProtKB - P34071 (ENTC2_STAAU)
Protein
Enterotoxin type C-2
Gene
entC2
Organism
Staphylococcus aureus
Status
Functioni
Staphylococcal enterotoxin that activates the host immune system by binding as unprocessed molecules to major histocompatibility (MHC) complex class II and T-cell receptor (TCR) molecules. In turn, this ternary complex activates a large number of T-lymphocytes initiating a systemic release of proinflammatory cytokines (PubMed:2210803, PubMed:19246739, PubMed:14559915). Causes also the intoxication staphylococcal food poisoning syndrome (PubMed:19246739).3 Publications
Cofactori
Zn2+Note: Possesses two zinc-binding sites. The zinc ion is necessary for the toxin interaction with MHC class II.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 36 | Zinc 1; shared with neighboring subunitCombined sources3 Publications | 1 | |
Metal bindingi | 74 | Zinc 2; via tele nitrogenCombined sources1 Publication | 1 | |
Metal bindingi | 98 | Zinc 2Combined sources1 Publication | 1 | |
Metal bindingi | 107 | Zinc 2Combined sources1 Publication | 1 | |
Metal bindingi | 110 | Zinc 1Combined sources3 Publications | 1 | |
Metal bindingi | 145 | Zinc 1; via pros nitrogenCombined sources3 Publications | 1 | |
Metal bindingi | 146 | Zinc 2Combined sources1 Publication | 1 | |
Metal bindingi | 149 | Zinc 1; via tele nitrogenCombined sources3 Publications | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- toxin activity Source: UniProtKB-KW
GO - Biological processi
- pathogenesis Source: UniProtKB-KW
Keywordsi
Molecular function | Enterotoxin, Superantigen, Toxin |
Biological process | Virulence |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Enterotoxin type C-2Alternative name(s): SEC2 |
Gene namesi | Name:entC2 |
Organismi | Staphylococcus aureus |
Taxonomic identifieri | 1280 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus |
Subcellular locationi
GO - Cellular componenti
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 74 | H → A: About 30% loss of T-cell stimulation. 1 Publication | 1 | |
Mutagenesisi | 145 | H → A: Complete loss of emetic activity. 1 Publication | 1 | |
Mutagenesisi | 149 | H → A: Complete loss of emetic activity. 1 Publication | 1 |
Protein family/group databases
Allergomei | 2141, Sta a SEC |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 27 | 1 PublicationAdd BLAST | 27 | |
ChainiPRO_0000035608 | 28 – 266 | Enterotoxin type C-2Add BLAST | 239 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 120 ↔ 137 | Combined sources2 Publications |
Keywords - PTMi
Disulfide bondInteractioni
Subunit structurei
Interacts with host MHC class II molecules composed of alpha/HLA-DRA and beta/HLA-DRB1 chains.
1 PublicationStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P34071 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P34071 |
Family & Domainsi
Sequence similaritiesi
Belongs to the staphylococcal/streptococcal toxin family.Curated
Keywords - Domaini
SignalFamily and domain databases
InterProi | View protein in InterPro IPR008992, Enterotoxin IPR006126, Staph/Strept_toxin_CS IPR016091, SuperAg_toxin_C IPR013307, Superantigen_bac IPR006123, Toxin_b-grasp_Staph/Strep IPR006177, Toxin_bac |
Pfami | View protein in Pfam PF02876, Stap_Strp_tox_C, 1 hit |
PRINTSi | PR00279, BACTRLTOXIN PR01898, SAGSUPRFAMLY |
SUPFAMi | SSF50203, SSF50203, 1 hit SSF54334, SSF54334, 1 hit |
PROSITEi | View protein in PROSITE PS00277, STAPH_STREP_TOXIN_1, 1 hit PS00278, STAPH_STREP_TOXIN_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P34071-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNKSRFISCV ILIFALILVL FTPNVLAESQ PDPTPDELHK SSEFTGTMGN
60 70 80 90 100
MKYLYDDHYV SATKVMSVDK FLAHDLIYNI SDKKLKNYDK VKTELLNEDL
110 120 130 140 150
AKKYKDEVVD VYGSNYYVNC YFSSKDNVGK VTGGKTCMYG GITKHEGNHF
160 170 180 190 200
DNGNLQNVLI RVYENKRNTI SFEVQTDKKS VTAQELDIKA RNFLINKKNL
210 220 230 240 250
YEFNSSPYET GYIKFIENNG NTFWYDMMPA PGDKFDQSKY LMMYNDNKTV
260
DSKSVKIEVH LTTKNG
Sequence databases
PIRi | A60114 |
RefSeqi | WP_001043552.1, NZ_WKII01000007.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
PIRi | A60114 |
RefSeqi | WP_001043552.1, NZ_WKII01000007.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1CQV | X-ray | 2.06 | A | 28-266 | [»] | |
1I4P | X-ray | 2.00 | A | 28-266 | [»] | |
1I4Q | X-ray | 2.20 | A | 28-266 | [»] | |
1I4R | X-ray | 2.10 | A | 28-266 | [»] | |
1I4X | X-ray | 2.40 | A | 28-266 | [»] | |
1SE2 | X-ray | 2.70 | A | 28-266 | [»] | |
1STE | X-ray | 2.00 | A | 28-266 | [»] | |
1UNS | X-ray | 2.00 | A | 28-266 | [»] | |
SMRi | P34071 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein family/group databases
Allergomei | 2141, Sta a SEC |
Miscellaneous databases
EvolutionaryTracei | P34071 |
PROi | PR:P34071 |
Family and domain databases
InterProi | View protein in InterPro IPR008992, Enterotoxin IPR006126, Staph/Strept_toxin_CS IPR016091, SuperAg_toxin_C IPR013307, Superantigen_bac IPR006123, Toxin_b-grasp_Staph/Strep IPR006177, Toxin_bac |
Pfami | View protein in Pfam PF02876, Stap_Strp_tox_C, 1 hit |
PRINTSi | PR00279, BACTRLTOXIN PR01898, SAGSUPRFAMLY |
SUPFAMi | SSF50203, SSF50203, 1 hit SSF54334, SSF54334, 1 hit |
PROSITEi | View protein in PROSITE PS00277, STAPH_STREP_TOXIN_1, 1 hit PS00278, STAPH_STREP_TOXIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ENTC2_STAAU | |
Accessioni | P34071Primary (citable) accession number: P34071 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | February 1, 1994 | |
Last modified: | December 2, 2020 | |
This is version 118 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families