Psmb4

Functionⁱ

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.By similarity

Catalytic activityⁱ

Cleavage of peptide bonds with very broad specificity.By similarity

GO - Molecular functionⁱ

threonine-type endopeptidase activity Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

proteolysis involved in cellular protein catabolic process Source: InterPro

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Hydrolase, Protease, Threonine protease

Molecular function

Hydrolase, Protease, Threonine protease

Protein family/group databases

MEROPS protease database More...MEROPSⁱ	T01.987

MEROPSⁱ

T01.987

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Proteasome subunit beta type-4 (EC:3.4.25.1By similarity) Alternative name(s): Macropain beta chain Multicatalytic endopeptidase complex beta chain Proteasome beta chain Proteasome chain 3 Short name: RN3
Gene namesⁱ	Name:Psmb4
Organismⁱ	Rattus norvegicus (Rat)
Taxonomic identifierⁱ	10116 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus
Proteomesⁱ	UP000002494 Componentⁱ: Unplaced

Organism-specific databases

Rat genome database More...RGDⁱ	61877 Psmb4

RGDⁱ

61877 Psmb4

Keywords - Cellular componentⁱ

Cytoplasm, Nucleus, Proteasome

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
PropeptideⁱPRO_0000026583	1 – 44	1 PublicationAdd BLAST		44
ChainⁱPRO_0000026584	45 – 263	Proteasome subunit beta type-4Add BLAST		219

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	1	N-acetylmethionineBy similarity	1
Modified residueⁱ	26	PhosphoserineBy similarity	1
Modified residueⁱ	101	PhosphotyrosineBy similarity	1

Keywords - PTMⁱ

Acetylation, Phosphoprotein, Zymogen

Proteomic databases

PaxDbⁱ	P34067
PeptideAtlas More...PeptideAtlasⁱ	P34067
PRIDEⁱ	P34067

2D gel databases

The World-2DPAGE database More...World-2DPAGEⁱ	0004:P34067

World-2DPAGEⁱ

0004:P34067

PTM databases

iPTMnetⁱ	P34067
PhosphoSitePlusⁱ	P34067

Expressionⁱ

Inductionⁱ

Up-regulated in cardiac hypertrophy and hypoxemia.1 Publication

Interactionⁱ

Subunit structureⁱ

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is a barrel-shaped complex made of 28 subunits that are arranged in four stacked rings. The two outer rings are each formed by seven alpha subunits, and the two inner rings are formed by seven beta subunits. The proteolytic activity is exerted by three beta-subunits PSMB5, PSMB6 and PSMB7. Forms a ternary complex with SMAD1 and OAZ1 before PSMB4 is incorporated into the 20S proteasome. Interacts with PRPF19.By similarity

Protein-protein interaction databases

IntActⁱ	P34067, 1 interactor
STRINGⁱ	10116.ENSRNOP00000028484

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P34067
SMRⁱ	P34067
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the peptidase T1B family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGⁱ	KOG0185 Eukaryota ENOG410YAMA LUCA
HOGENOMⁱ	HOG000181719
HOVERGENⁱ	HBG018194
InParanoidⁱ	P34067
PhylomeDBⁱ	P34067

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd03760 proteasome_beta_type_4, 1 hit
Gene3Dⁱ	3.60.20.10, 1 hit
InterProⁱ	View protein in InterPro IPR029055 Ntn_hydrolases_N IPR016295 Proteasome_beta4 IPR016050 Proteasome_bsu_CS IPR001353 Proteasome_sua/b IPR023333 Proteasome_suB-type
PANTHERⁱ	PTHR11599:SF5 PTHR11599:SF5, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00227 Proteasome, 1 hit
PIRSFⁱ	PIRSF001213 Psome_endopept_beta, 1 hit
SUPFAMⁱ	SSF56235 SSF56235, 1 hit
PROSITEⁱ	View protein in PROSITE PS00854 PROTEASOME_BETA_1, 1 hit PS51476 PROTEASOME_BETA_2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

P34067-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MEAFWESRTG HWAGGPAPGQ FYRVPSTPSC LMDPMSAPAR PITRTQNPMV 
        60         70         80         90        100
TGTSVLGVKF DCGVVIAADM LGSYGSLARF RNISRIMRVN DSTMLGASGD 
       110        120        130        140        150
YADFQYLKQV LGQMVIDEEL FGDGHSYSPR AIHSWLTRAM YSRRSKMNPL 
       160        170        180        190        200
WNTKVIGGYA GGESFLGYVD MLGVAYEAPS LATGYGAYLA QPLLREVLEK 
       210        220        230        240        250
QPVLSQTEAR ELVERCMRVL YYRDARSYNR FQVATVTEKG VEIEGPLSAQ 
       260 
TNWDIAHMIS GFE

Length:263

Mass (Da):29,197

Last modified:December 15, 1998 - v2

Checksum:ⁱC8FCB91265F8FB90

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	41	P → S in AAA42054 (PubMed:8482379).Curated	1
Sequence conflictⁱ	50	V → L in AAA42054 (PubMed:8482379).Curated	1
Sequence conflictⁱ	60	F → S in AAB47113 (PubMed:8645151).Curated	1
Sequence conflictⁱ	80	F → L in AAB47113 (PubMed:8645151).Curated	1
Sequence conflictⁱ	82	N → I in AAA42054 (PubMed:8482379).Curated	1
Sequence conflictⁱ	86	I → F in AAB47113 (PubMed:8645151).Curated	1
Sequence conflictⁱ	89	V → L in AAB47113 (PubMed:8645151).Curated	1
Sequence conflictⁱ	121	F → L AA sequence (Ref. 4) Curated	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	S82190 mRNA Translation: AAB47113.2 L17127 mRNA Translation: AAA42054.1
PIRⁱ	S09084 S32507
UniGeneⁱ	Rn.6169

Genome annotation databases

UCSC genome browser More...UCSCⁱ	RGD:61877 rat

UCSCⁱ

RGD:61877 rat

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length
P34067	Proteasome subunit beta type-4	)	HUMAN		264
Proteasome subunit beta		PANPA		264
Proteasome subunit beta		GORGO		264
UPI000719FFB4		EQUAS		309
UPI0003ACCA02		EQUPR		306
+122

Protein	Similar proteins		Species	Score	Length
P34067	Proteasome subunit beta type-4	)	HUMAN		264
Proteasome subunit beta		PANPA		264
Proteasome subunit beta		GORGO		264
UPI000719FFB4		EQUAS		309
UPI0003ACCA02		EQUPR		306
+261

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	S82190 mRNA Translation: AAB47113.2 L17127 mRNA Translation: AAA42054.1
PIRⁱ	S09084 S32507
UniGeneⁱ	Rn.6169

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	6EPC	electron microscopy	12.30	7	1-263	[»]
	6EPD	electron microscopy	15.40	7	1-263	[»]
	6EPE	electron microscopy	12.80	7	1-263	[»]
	6EPF	electron microscopy	11.80	7	1-263	[»]
ProteinModelPortalⁱ	P34067
SMRⁱ	P34067
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

IntActⁱ	P34067, 1 interactor
STRINGⁱ	10116.ENSRNOP00000028484

Protein family/group databases

MEROPS protease database More...MEROPSⁱ	T01.987

MEROPSⁱ

T01.987

PTM databases

iPTMnetⁱ	P34067
PhosphoSitePlusⁱ	P34067

2D gel databases

The World-2DPAGE database More...World-2DPAGEⁱ	0004:P34067

World-2DPAGEⁱ

0004:P34067

Proteomic databases

PaxDbⁱ	P34067
PeptideAtlas More...PeptideAtlasⁱ	P34067
PRIDEⁱ	P34067

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

UCSC genome browser More...UCSCⁱ	RGD:61877 rat

UCSCⁱ

RGD:61877 rat

Organism-specific databases

Rat genome database More...RGDⁱ	61877 Psmb4

RGDⁱ

61877 Psmb4

Phylogenomic databases

eggNOGⁱ	KOG0185 Eukaryota ENOG410YAMA LUCA
HOGENOMⁱ	HOG000181719
HOVERGENⁱ	HBG018194
InParanoidⁱ	P34067
PhylomeDBⁱ	P34067

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P34067

PROⁱ

PR:P34067

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd03760 proteasome_beta_type_4, 1 hit
Gene3Dⁱ	3.60.20.10, 1 hit
InterProⁱ	View protein in InterPro IPR029055 Ntn_hydrolases_N IPR016295 Proteasome_beta4 IPR016050 Proteasome_bsu_CS IPR001353 Proteasome_sua/b IPR023333 Proteasome_suB-type
PANTHERⁱ	PTHR11599:SF5 PTHR11599:SF5, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00227 Proteasome, 1 hit
PIRSFⁱ	PIRSF001213 Psome_endopept_beta, 1 hit
SUPFAMⁱ	SSF56235 SSF56235, 1 hit
PROSITEⁱ	View protein in PROSITE PS00854 PROTEASOME_BETA_1, 1 hit PS51476 PROTEASOME_BETA_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	PSB4_RAT
Accessionⁱ	P34067Primary (citable) accession number: P34067 Secondary accession number(s): P28071, P97719
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
	Last sequence update:	December 15, 1998
	Last modified:	July 18, 2018
	This is version 146 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

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UniProtKB - P34067 (PSB4_RAT)

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Proteasome subunit beta type-4

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Other locations

Nucleus

Other locations

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

2D gel databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

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Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ