UniProtKB - P34067 (PSB4_RAT)
Proteasome subunit beta type-4
Psmb4
Functioni
GO - Molecular functioni
- endopeptidase activity Source: GO_Central
- lipopolysaccharide binding Source: RGD
- threonine-type endopeptidase activity Source: UniProtKB-KW
GO - Biological processi
- negative regulation of inflammatory response to antigenic stimulus Source: RGD
- proteasomal protein catabolic process Source: GO_Central
- proteasomal ubiquitin-independent protein catabolic process Source: GO_Central
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
Enzyme and pathway databases
Reactomei | R-RNO-1169091, Activation of NF-kappaB in B cells R-RNO-1234176, Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha R-RNO-1236978, Cross-presentation of soluble exogenous antigens (endosomes) R-RNO-174084, Autodegradation of Cdh1 by Cdh1:APC/C R-RNO-174154, APC/C:Cdc20 mediated degradation of Securin R-RNO-174178, APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 R-RNO-174184, Cdc20:Phospho-APC/C mediated degradation of Cyclin A R-RNO-187577, SCF(Skp2)-mediated degradation of p27/p21 R-RNO-195253, Degradation of beta-catenin by the destruction complex R-RNO-202424, Downstream TCR signaling R-RNO-2467813, Separation of Sister Chromatids R-RNO-2871837, FCERI mediated NF-kB activation R-RNO-349425, Autodegradation of the E3 ubiquitin ligase COP1 R-RNO-350562, Regulation of ornithine decarboxylase (ODC) R-RNO-382556, ABC-family proteins mediated transport R-RNO-450408, AUF1 (hnRNP D0) binds and destabilizes mRNA R-RNO-4608870, Asymmetric localization of PCP proteins R-RNO-4641257, Degradation of AXIN R-RNO-4641258, Degradation of DVL R-RNO-5358346, Hedgehog ligand biogenesis R-RNO-5607761, Dectin-1 mediated noncanonical NF-kB signaling R-RNO-5607764, CLEC7A (Dectin-1) signaling R-RNO-5610780, Degradation of GLI1 by the proteasome R-RNO-5610785, GLI3 is processed to GLI3R by the proteasome R-RNO-5632684, Hedgehog 'on' state R-RNO-5658442, Regulation of RAS by GAPs R-RNO-5668541, TNFR2 non-canonical NF-kB pathway R-RNO-5676590, NIK-->noncanonical NF-kB signaling R-RNO-5687128, MAPK6/MAPK4 signaling R-RNO-5689603, UCH proteinases R-RNO-5689880, Ub-specific processing proteases R-RNO-68827, CDT1 association with the CDC6:ORC:origin complex R-RNO-68949, Orc1 removal from chromatin R-RNO-69017, CDK-mediated phosphorylation and removal of Cdc6 R-RNO-69481, G2/M Checkpoints R-RNO-69601, Ubiquitin Mediated Degradation of Phosphorylated Cdc25A R-RNO-75815, Ubiquitin-dependent degradation of Cyclin D R-RNO-8852276, The role of GTSE1 in G2/M progression after G2 checkpoint R-RNO-8854050, FBXL7 down-regulates AURKA during mitotic entry and in early mitosis R-RNO-8939236, RUNX1 regulates transcription of genes involved in differentiation of HSCs R-RNO-8939902, Regulation of RUNX2 expression and activity R-RNO-8941858, Regulation of RUNX3 expression and activity R-RNO-8948751, Regulation of PTEN stability and activity R-RNO-8951664, Neddylation R-RNO-9020702, Interleukin-1 signaling R-RNO-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Protein family/group databases
MEROPSi | T01.987 |
Names & Taxonomyi
Protein namesi | Recommended name: Proteasome subunit beta type-4Alternative name(s): Macropain beta chain Multicatalytic endopeptidase complex beta chain Proteasome beta chain Proteasome chain 3 Short name: RN3 |
Gene namesi | Name:Psmb4 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 61877, Psmb4 |
Subcellular locationi
Cytoskeleton
- ciliary basal body Source: RGD
Nucleus
- nucleus Source: GO_Central
Other locations
- cytoplasm Source: GO_Central
- proteasome complex Source: RGD
- proteasome core complex Source: UniProtKB
- proteasome core complex, beta-subunit complex Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Nucleus, ProteasomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
PropeptideiPRO_0000026583 | 1 – 44 | 1 PublicationAdd BLAST | 44 | |
ChainiPRO_0000026584 | 45 – 263 | Proteasome subunit beta type-4Add BLAST | 219 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 26 | PhosphoserineBy similarity | 1 | |
Modified residuei | 101 | PhosphotyrosineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
jPOSTi | P34067 |
PaxDbi | P34067 |
PeptideAtlasi | P34067 |
PRIDEi | P34067 |
2D gel databases
World-2DPAGEi | 0004:P34067 |
PTM databases
iPTMneti | P34067 |
PhosphoSitePlusi | P34067 |
Expressioni
Inductioni
Interactioni
Subunit structurei
The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is a barrel-shaped complex made of 28 subunits that are arranged in four stacked rings. The two outer rings are each formed by seven alpha subunits, and the two inner rings are formed by seven beta subunits. The proteolytic activity is exerted by three beta-subunits PSMB5, PSMB6 and PSMB7.
Forms a ternary complex with SMAD1 and OAZ1 before PSMB4 is incorporated into the 20S proteasome.
Interacts with PRPF19.
By similarityProtein-protein interaction databases
BioGRIDi | 248653, 1 interactor |
IntActi | P34067, 1 interactor |
STRINGi | 10116.ENSRNOP00000028484 |
Structurei
Secondary structure
3D structure databases
SMRi | P34067 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0185, Eukaryota |
InParanoidi | P34067 |
PhylomeDBi | P34067 |
Family and domain databases
CDDi | cd03760, proteasome_beta_type_4, 1 hit |
Gene3Di | 3.60.20.10, 1 hit |
InterProi | View protein in InterPro IPR029055, Ntn_hydrolases_N IPR016295, Proteasome_beta4 IPR016050, Proteasome_bsu_CS IPR001353, Proteasome_sua/b IPR023333, Proteasome_suB-type |
PANTHERi | PTHR11599:SF5, PTHR11599:SF5, 1 hit |
Pfami | View protein in Pfam PF00227, Proteasome, 1 hit |
PIRSFi | PIRSF001213, Psome_endopept_beta, 1 hit |
SUPFAMi | SSF56235, SSF56235, 1 hit |
PROSITEi | View protein in PROSITE PS00854, PROTEASOME_BETA_1, 1 hit PS51476, PROTEASOME_BETA_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MEAFWESRTG HWAGGPAPGQ FYRVPSTPSC LMDPMSAPAR PITRTQNPMV
60 70 80 90 100
TGTSVLGVKF DCGVVIAADM LGSYGSLARF RNISRIMRVN DSTMLGASGD
110 120 130 140 150
YADFQYLKQV LGQMVIDEEL FGDGHSYSPR AIHSWLTRAM YSRRSKMNPL
160 170 180 190 200
WNTKVIGGYA GGESFLGYVD MLGVAYEAPS LATGYGAYLA QPLLREVLEK
210 220 230 240 250
QPVLSQTEAR ELVERCMRVL YYRDARSYNR FQVATVTEKG VEIEGPLSAQ
260
TNWDIAHMIS GFE
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketG3V8U9 | G3V8U9_RAT | Proteasome subunit beta | Psmb4 | 232 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 41 | P → S in AAA42054 (PubMed:8482379).Curated | 1 | |
Sequence conflicti | 50 | V → L in AAA42054 (PubMed:8482379).Curated | 1 | |
Sequence conflicti | 60 | F → S in AAB47113 (PubMed:8645151).Curated | 1 | |
Sequence conflicti | 80 | F → L in AAB47113 (PubMed:8645151).Curated | 1 | |
Sequence conflicti | 82 | N → I in AAA42054 (PubMed:8482379).Curated | 1 | |
Sequence conflicti | 86 | I → F in AAB47113 (PubMed:8645151).Curated | 1 | |
Sequence conflicti | 89 | V → L in AAB47113 (PubMed:8645151).Curated | 1 | |
Sequence conflicti | 121 | F → L AA sequence (Ref. 4) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S82190 mRNA Translation: AAB47113.2 L17127 mRNA Translation: AAA42054.1 |
PIRi | S09084 S32507 |
Genome annotation databases
UCSCi | RGD:61877, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S82190 mRNA Translation: AAB47113.2 L17127 mRNA Translation: AAA42054.1 |
PIRi | S09084 S32507 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6EPC | electron microscopy | 12.30 | 7 | 1-263 | [»] | |
6EPD | electron microscopy | 15.40 | 7 | 1-263 | [»] | |
6EPE | electron microscopy | 12.80 | 7 | 1-263 | [»] | |
6EPF | electron microscopy | 11.80 | 7 | 1-263 | [»] | |
6TU3 | electron microscopy | 2.70 | N/b | 1-263 | [»] | |
SMRi | P34067 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 248653, 1 interactor |
IntActi | P34067, 1 interactor |
STRINGi | 10116.ENSRNOP00000028484 |
Protein family/group databases
MEROPSi | T01.987 |
PTM databases
iPTMneti | P34067 |
PhosphoSitePlusi | P34067 |
2D gel databases
World-2DPAGEi | 0004:P34067 |
Proteomic databases
jPOSTi | P34067 |
PaxDbi | P34067 |
PeptideAtlasi | P34067 |
PRIDEi | P34067 |
Genome annotation databases
UCSCi | RGD:61877, rat |
Organism-specific databases
RGDi | 61877, Psmb4 |
Phylogenomic databases
eggNOGi | KOG0185, Eukaryota |
InParanoidi | P34067 |
PhylomeDBi | P34067 |
Enzyme and pathway databases
Reactomei | R-RNO-1169091, Activation of NF-kappaB in B cells R-RNO-1234176, Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha R-RNO-1236978, Cross-presentation of soluble exogenous antigens (endosomes) R-RNO-174084, Autodegradation of Cdh1 by Cdh1:APC/C R-RNO-174154, APC/C:Cdc20 mediated degradation of Securin R-RNO-174178, APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 R-RNO-174184, Cdc20:Phospho-APC/C mediated degradation of Cyclin A R-RNO-187577, SCF(Skp2)-mediated degradation of p27/p21 R-RNO-195253, Degradation of beta-catenin by the destruction complex R-RNO-202424, Downstream TCR signaling R-RNO-2467813, Separation of Sister Chromatids R-RNO-2871837, FCERI mediated NF-kB activation R-RNO-349425, Autodegradation of the E3 ubiquitin ligase COP1 R-RNO-350562, Regulation of ornithine decarboxylase (ODC) R-RNO-382556, ABC-family proteins mediated transport R-RNO-450408, AUF1 (hnRNP D0) binds and destabilizes mRNA R-RNO-4608870, Asymmetric localization of PCP proteins R-RNO-4641257, Degradation of AXIN R-RNO-4641258, Degradation of DVL R-RNO-5358346, Hedgehog ligand biogenesis R-RNO-5607761, Dectin-1 mediated noncanonical NF-kB signaling R-RNO-5607764, CLEC7A (Dectin-1) signaling R-RNO-5610780, Degradation of GLI1 by the proteasome R-RNO-5610785, GLI3 is processed to GLI3R by the proteasome R-RNO-5632684, Hedgehog 'on' state R-RNO-5658442, Regulation of RAS by GAPs R-RNO-5668541, TNFR2 non-canonical NF-kB pathway R-RNO-5676590, NIK-->noncanonical NF-kB signaling R-RNO-5687128, MAPK6/MAPK4 signaling R-RNO-5689603, UCH proteinases R-RNO-5689880, Ub-specific processing proteases R-RNO-68827, CDT1 association with the CDC6:ORC:origin complex R-RNO-68949, Orc1 removal from chromatin R-RNO-69017, CDK-mediated phosphorylation and removal of Cdc6 R-RNO-69481, G2/M Checkpoints R-RNO-69601, Ubiquitin Mediated Degradation of Phosphorylated Cdc25A R-RNO-75815, Ubiquitin-dependent degradation of Cyclin D R-RNO-8852276, The role of GTSE1 in G2/M progression after G2 checkpoint R-RNO-8854050, FBXL7 down-regulates AURKA during mitotic entry and in early mitosis R-RNO-8939236, RUNX1 regulates transcription of genes involved in differentiation of HSCs R-RNO-8939902, Regulation of RUNX2 expression and activity R-RNO-8941858, Regulation of RUNX3 expression and activity R-RNO-8948751, Regulation of PTEN stability and activity R-RNO-8951664, Neddylation R-RNO-9020702, Interleukin-1 signaling R-RNO-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
PROi | PR:P34067 |
Family and domain databases
CDDi | cd03760, proteasome_beta_type_4, 1 hit |
Gene3Di | 3.60.20.10, 1 hit |
InterProi | View protein in InterPro IPR029055, Ntn_hydrolases_N IPR016295, Proteasome_beta4 IPR016050, Proteasome_bsu_CS IPR001353, Proteasome_sua/b IPR023333, Proteasome_suB-type |
PANTHERi | PTHR11599:SF5, PTHR11599:SF5, 1 hit |
Pfami | View protein in Pfam PF00227, Proteasome, 1 hit |
PIRSFi | PIRSF001213, Psome_endopept_beta, 1 hit |
SUPFAMi | SSF56235, SSF56235, 1 hit |
PROSITEi | View protein in PROSITE PS00854, PROTEASOME_BETA_1, 1 hit PS51476, PROTEASOME_BETA_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PSB4_RAT | |
Accessioni | P34067Primary (citable) accession number: P34067 Secondary accession number(s): P28071, P97719 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | December 15, 1998 | |
Last modified: | February 10, 2021 | |
This is version 159 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families