UniProtKB - P34057 (RECO_MOUSE)
Protein
Recoverin
Gene
Rcvrn
Organism
Mus musculus (Mouse)
Status
Functioni
Acts as a calcium sensor and regulates phototransduction of cone and rod photoreceptor cells (By similarity). Modulates light sensitivity of cone photoreceptor in dark and dim conditions (PubMed:25673692). In response to high Ca2+ levels induced by low light levels, prolongs RHO/rhodopsin activation in rod photoreceptor cells by binding to and inhibiting GRK1-mediated phosphorylation of RHO/rhodopsin (By similarity). Plays a role in scotopic vision/enhances vision in dim light by enhancing signal transfer between rod photoreceptors and rod bipolar cells (PubMed:15882641). Improves rod photoreceptor sensitivity in dim light and mediates response of rod photoreceptors to facilitate detection of change and motion in bright light (PubMed:29435986).By similarity3 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 74 | Calcium 1By similarity | 1 | |
Metal bindingi | 76 | Calcium 1By similarity | 1 | |
Metal bindingi | 78 | Calcium 1By similarity | 1 | |
Metal bindingi | 80 | Calcium 1; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 85 | Calcium 1By similarity | 1 | |
Metal bindingi | 110 | Calcium 2By similarity | 1 | |
Metal bindingi | 112 | Calcium 2By similarity | 1 | |
Metal bindingi | 114 | Calcium 2By similarity | 1 | |
Metal bindingi | 116 | Calcium 2; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 121 | Calcium 2By similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Calcium bindingi | 74 – 85 | 1; low affinityPROSITE-ProRule annotationAdd BLAST | 12 | |
Calcium bindingi | 110 – 121 | 2; high affinityPROSITE-ProRule annotationAdd BLAST | 12 |
GO - Molecular functioni
- calcium ion binding Source: MGI
GO - Biological processi
- phototransduction Source: MGI
- regulation of calcium ion transport Source: MGI
- visual perception Source: MGI
Keywordsi
Biological process | Sensory transduction, Vision |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
Reactomei | R-MMU-2514859, Inactivation, recovery and regulation of the phototransduction cascade |
Names & Taxonomyi
Protein namesi | Recommended name: RecoverinAlternative name(s): 23 kDa photoreceptor cell-specific protein Cancer-associated retinopathy protein Short name: Protein CAR |
Gene namesi | Name:Rcvrn Synonyms:Rcv1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:97883, Rcvrn |
Subcellular locationi
Other locations
- Photoreceptor inner segment 3 Publications
- photoreceptor outer segment 3 Publications
- Photoreceptor outer segment membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity
- Perikaryon 1 Publication
Note: Primarily expressed in the inner segments of light-adapted rod photoreceptors, approximately 10% of which translocates from photoreceptor outer segments upon light stimulation (PubMed:15961391). Targeting of myristoylated protein to rod photoreceptor outer segments is calcium dependent (By similarity).By similarity1 Publication
Other locations
- dendrite Source: MGI
- membrane Source: UniProtKB-KW
- perikaryon Source: UniProtKB-SubCell
- photoreceptor inner segment Source: UniProtKB-SubCell
- photoreceptor outer segment Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell projection, MembranePathology & Biotechi
Disruption phenotypei
Knockout mice exhibit reduced scotopic vision acuity, however do not show rod photoreceptor degeneration (PubMed:15882641). Rod photoreceptors have a higher visual response threshold resulting in a 4-fold enhancement in sensitivity to low light intensity, with no change in pupil size (PubMed:15882641). Dark adapted rod photoreceptors have a reduced recovery time following stimulus, a reduced adaption time to background light and sustained photoreceptor signaling response following stimulus (PubMed:29435986). Rod photoreceptors have an increased sensitivity to stimulus in saturating background conditions (PubMed:29435986). Mice show a decrease in response, sensitivity and photoreceptor recovery to light stimulus in dark-adapted cone photoreceptors (PubMed:25673692). Rcvrn, Guca1a, and Guca1b triple knockout mice have a sustained photoreceptor signaling response following stimulus during light response in rod photoreceptors (PubMed:29435986).3 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000073760 | 2 – 202 | RecoverinAdd BLAST | 201 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycineBy similarity | 1 | |
Modified residuei | 39 | Cysteine sulfenic acid (-SOH)By similarity | 1 | |
Disulfide bondi | 39 | Interchain, redox-activeBy similarity |
Post-translational modificationi
The N-terminal glycine is linked to one of four different types of acyl groups. The most abundant is myristoleate (14:1), but 14:0, 14:2, and 12:0 acyl residues are also present (By similarity). The Ca2+ induced exposure of the myristoyl group, known as the calcium-myristoyl switch, promotes RCVRN binding to the photoreceptor cell membranes only when intracellular Ca2+ concentration is high (By similarity).By similarity
Oxidation on Cys-39 occurs in response to prolonged intense illumination and results in the formation of disulfide homodimers, and to a lesser extent disulfide-linked heterodimers.By similarity
Keywords - PTMi
Disulfide bond, Lipoprotein, Myristate, OxidationProteomic databases
PaxDbi | P34057 |
PRIDEi | P34057 |
PTM databases
PhosphoSitePlusi | P34057 |
Expressioni
Tissue specificityi
Expressed in rod photoreceptors in the retina (at protein level).3 Publications
Developmental stagei
At postnatal day 11 abundantly expressed in the retina outer plexiform layer, outer nuclear layer, and outer limiting membrane, with weak expression in the inner nuclear layer and inner plexiform layer (PubMed:28151698). At postnatal day 22 abundantly expressed in the retina inner nuclear layer, outer plexiform layer, outer nuclear layer, outer limiting membrane, outer segment, and inner segment (PubMed:28151698).1 Publication
Gene expression databases
Bgeei | ENSMUSG00000020907, Expressed in retina and 48 other tissues |
ExpressionAtlasi | P34057, baseline and differential |
Genevisiblei | P34057, MM |
Interactioni
Subunit structurei
Homodimer; disulfide-linked (By similarity). Homodimerization is caused by prolonged intense illumination (By similarity). May form a complex composed of RHO, GRK1 and RCVRN in a Ca2+-dependent manner; RCVRN prevents the interaction between GRK1 and RHO (By similarity).
Interacts (via C-terminus) with GRK1 (via N-terminus); the interaction is Ca2+-dependent (By similarity).
By similaritySites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 192 | Interaction with GRK1By similarity | 1 |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000021290 |
Miscellaneous databases
RNActi | P34057, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 24 – 59 | EF-hand 1PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 61 – 96 | EF-hand 2PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 97 – 132 | EF-hand 3PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 147 – 182 | EF-hand 4PROSITE-ProRule annotationAdd BLAST | 36 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 189 – 192 | Interaction with GRK1By similarity | 4 |
Domaini
EF-hand 2 and EF-hand 3 domains are the low-affinity and the high-affinity calcium binding sites, respectively. EF-hand 1 and EF-hand 4 domains do not bind calcium due to substitutions that disrupt their respective Ca2+ binding loops. The cooperative binding of calcium to the EF-hand 2 domain following EF-hand 3 domain calcium binding requires myristoylation (By similarity). Calcium binding to the 2 EF-hand domains induces exposure of the myristoyl group through a protein conformation change, this process known as the calcium-myristoyl switch facilitates binding to photoreceptor cell membranes (By similarity).By similarity
Sequence similaritiesi
Belongs to the recoverin family.Curated
Keywords - Domaini
Redox-active center, RepeatPhylogenomic databases
eggNOGi | KOG0044, Eukaryota |
GeneTreei | ENSGT00940000159441 |
HOGENOMi | CLU_072366_1_0_1 |
InParanoidi | P34057 |
OMAi | LIQYDEP |
PhylomeDBi | P34057 |
TreeFami | TF300009 |
Family and domain databases
CDDi | cd00051, EFh, 2 hits |
InterProi | View protein in InterPro IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR028846, Recoverin |
PANTHERi | PTHR23055, PTHR23055, 1 hit |
Pfami | View protein in Pfam PF13499, EF-hand_7, 1 hit PF13833, EF-hand_8, 1 hit |
SMARTi | View protein in SMART SM00054, EFh, 2 hits |
SUPFAMi | SSF47473, SSF47473, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 2 hits PS50222, EF_HAND_2, 4 hits |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P34057-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGNSKSGALS KEILEELQLN TKFTEEELSA WYQSFLKECP SGRITRQEFE
60 70 80 90 100
SIYSKFFPDS DPKAYAQHVF RSFDANSDGT LDFKEYVIAL HMTTAGKPTQ
110 120 130 140 150
KLEWAFSLYD VDGNGTISKN EVLEIVMAIF KMIKPEDVKL LPDDENTPEK
160 170 180 190 200
RAEKIWAFFG KKEDDKLTEE EFIEGTLANK EILRLIQFEP QKVKERIKEK
KQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X66196 mRNA Translation: CAA46958.1 |
CCDSi | CCDS24859.1 |
PIRi | S21155 |
RefSeqi | NP_033064.1, NM_009038.2 |
Genome annotation databases
Ensembli | ENSMUST00000021290; ENSMUSP00000021290; ENSMUSG00000020907 |
GeneIDi | 19674 |
KEGGi | mmu:19674 |
UCSCi | uc007jmz.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X66196 mRNA Translation: CAA46958.1 |
CCDSi | CCDS24859.1 |
PIRi | S21155 |
RefSeqi | NP_033064.1, NM_009038.2 |
3D structure databases
SMRi | P34057 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000021290 |
PTM databases
PhosphoSitePlusi | P34057 |
Proteomic databases
PaxDbi | P34057 |
PRIDEi | P34057 |
Protocols and materials databases
Antibodypediai | 12699, 480 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000021290; ENSMUSP00000021290; ENSMUSG00000020907 |
GeneIDi | 19674 |
KEGGi | mmu:19674 |
UCSCi | uc007jmz.2, mouse |
Organism-specific databases
CTDi | 5957 |
MGIi | MGI:97883, Rcvrn |
Phylogenomic databases
eggNOGi | KOG0044, Eukaryota |
GeneTreei | ENSGT00940000159441 |
HOGENOMi | CLU_072366_1_0_1 |
InParanoidi | P34057 |
OMAi | LIQYDEP |
PhylomeDBi | P34057 |
TreeFami | TF300009 |
Enzyme and pathway databases
Reactomei | R-MMU-2514859, Inactivation, recovery and regulation of the phototransduction cascade |
Miscellaneous databases
BioGRID-ORCSi | 19674, 1 hit in 17 CRISPR screens |
PROi | PR:P34057 |
RNActi | P34057, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000020907, Expressed in retina and 48 other tissues |
ExpressionAtlasi | P34057, baseline and differential |
Genevisiblei | P34057, MM |
Family and domain databases
CDDi | cd00051, EFh, 2 hits |
InterProi | View protein in InterPro IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR028846, Recoverin |
PANTHERi | PTHR23055, PTHR23055, 1 hit |
Pfami | View protein in Pfam PF13499, EF-hand_7, 1 hit PF13833, EF-hand_8, 1 hit |
SMARTi | View protein in SMART SM00054, EFh, 2 hits |
SUPFAMi | SSF47473, SSF47473, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 2 hits PS50222, EF_HAND_2, 4 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RECO_MOUSE | |
Accessioni | P34057Primary (citable) accession number: P34057 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 154 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families