UniProtKB - P34056 (AP2A_MOUSE)
Transcription factor AP-2-alpha
Tfap2a
Functioni
Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation. Associates with chromatin to the PITX2 P1 promoter region.
1 PublicationGO - Molecular functioni
- chromatin binding Source: UniProtKB
- cis-regulatory region sequence-specific DNA binding Source: MGI
- DNA binding Source: MGI
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: UniProtKB
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: UniProtKB
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: MGI
- identical protein binding Source: MGI
- nuclear receptor corepressor activity Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: MGI
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: NTNU_SB
- sequence-specific DNA binding Source: MGI
- sequence-specific double-stranded DNA binding Source: MGI
- transcription cis-regulatory region binding Source: UniProtKB
- transcription coactivator activity Source: UniProtKB
- transcription corepressor activity Source: UniProtKB
GO - Biological processi
- anatomical structure development Source: GO_Central
- anterior neuropore closure Source: MGI
- basement membrane organization Source: MGI
- bone morphogenesis Source: UniProtKB
- cellular response to iron ion Source: UniProtKB
- cornea development in camera-type eye Source: MGI
- embryonic body morphogenesis Source: MGI
- embryonic camera-type eye morphogenesis Source: MGI
- embryonic cranial skeleton morphogenesis Source: UniProtKB
- embryonic forelimb morphogenesis Source: UniProtKB
- embryonic pattern specification Source: MGI
- epidermis morphogenesis Source: MGI
- eyelid development in camera-type eye Source: UniProtKB
- face morphogenesis Source: MGI
- forebrain neuron development Source: MGI
- forelimb morphogenesis Source: MGI
- inner ear morphogenesis Source: UniProtKB
- keratinocyte development Source: MGI
- kidney development Source: UniProtKB
- lens induction in camera-type eye Source: MGI
- lens morphogenesis in camera-type eye Source: MGI
- metanephric nephron development Source: UniProtKB
- negative regulation of apoptotic process Source: UniProtKB
- negative regulation of cell population proliferation Source: MGI
- negative regulation of epidermal growth factor receptor signaling pathway Source: MGI
- negative regulation of neuron apoptotic process Source: MGI
- negative regulation of reactive oxygen species metabolic process Source: UniProtKB
- negative regulation of transcription, DNA-templated Source: MGI
- negative regulation of transcription by competitive promoter binding Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: UniProtKB
- nervous system development Source: UniProtKB
- neural crest cell development Source: MGI
- neural tube closure Source: MGI
- oculomotor nerve formation Source: UniProtKB
- optic cup structural organization Source: UniProtKB
- optic vesicle morphogenesis Source: UniProtKB
- outflow tract morphogenesis Source: MGI
- positive regulation of bone mineralization Source: UniProtKB
- positive regulation of cell migration Source: MGI
- positive regulation of gene expression Source: UniProtKB
- positive regulation of neuron apoptotic process Source: MGI
- positive regulation of tooth mineralization Source: UniProtKB
- positive regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: UniProtKB
- regulation of cell differentiation Source: MGI
- regulation of cell population proliferation Source: GO_Central
- regulation of neuron differentiation Source: MGI
- regulation of transcription, DNA-templated Source: MGI
- regulation of transcription by RNA polymerase II Source: MGI
- response to lipopolysaccharide Source: MGI
- roof of mouth development Source: UniProtKB
- sensory organ development Source: UniProtKB
- sensory perception of sound Source: UniProtKB
- skeletal system morphogenesis Source: MGI
- skin development Source: MGI
- sympathetic nervous system development Source: MGI
- trigeminal nerve development Source: UniProtKB
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-8864260, Transcriptional regulation by the AP-2 (TFAP2) family of transcription factors R-MMU-8866904, Negative regulation of activity of TFAP2 (AP-2) family transcription factors R-MMU-8866907, Activation of the TFAP2 (AP-2) family of transcription factors R-MMU-8869496, TFAP2A acts as a transcriptional repressor during retinoic acid induced cell differentiation |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription factor AP-2-alphaShort name: AP2-alpha Alternative name(s): AP-2 transcription factor Activating enhancer-binding protein 2-alpha Activator protein 2 Short name: AP-2 |
Gene namesi | Name:Tfap2a Synonyms:Ap2tf, Tcfap2a |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:104671, Tfap2a |
Subcellular locationi
Nucleus
Cytosol
- cytosol Source: MGI
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: MGI
Plasma Membrane
- clathrin-coated pit Source: MGI
Other locations
- transcription regulator complex Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000184797 | 1 – 437 | Transcription factor AP-2-alphaAdd BLAST | 437 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 10 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternateBy similarity | ||
Cross-linki | 10 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Cross-linki | 177 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 184 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 239 | Phosphoserine; by PKABy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
MaxQBi | P34056 |
PaxDbi | P34056 |
PeptideAtlasi | P34056 |
PRIDEi | P34056 |
ProteomicsDBi | 296054 [P34056-1] 296055 [P34056-2] 296056 [P34056-3] 296057 [P34056-4] |
PTM databases
iPTMneti | P34056 |
PhosphoSitePlusi | P34056 |
Expressioni
Developmental stagei
Inductioni
Interactioni
Subunit structurei
Binds DNA as a dimer. Can form homodimers or heterodimers with other AP-2 family members.
Interacts with WWOX.
Interacts with UBE2I.
Interacts with RALBP1 in a complex also containing EPN1 and NUMB during interphase and mitosis (By similarity).
Interacts with CITED4.
Interacts with KCTD1; this interaction represses transcription activation.
Interacts (via C-terminus) with CITED2 (via C-terminus); the interaction stimulates TFAP2A-transcriptional activation.
Interacts (via N-terminus) with EP300 (via N-terminus); the interaction requires CITED2 (By similarity).
Interacts with KCTD15; this interaction inhibits TFAP2A transcriptional activation (By similarity).
By similarityGO - Molecular functioni
- identical protein binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 204011, 7 interactors |
CORUMi | P34056 |
IntActi | P34056, 1 interactor |
STRINGi | 10090.ENSMUSP00000105822 |
Miscellaneous databases
RNActi | P34056, protein |
Structurei
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 14 – 107 | DisorderedSequence analysisAdd BLAST | 94 | |
Regioni | 280 – 410 | H-S-H (helix-span-helix), dimerizationBy similarityAdd BLAST | 131 | |
Regioni | 414 – 437 | DisorderedSequence analysisAdd BLAST | 24 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 57 – 62 | PPxY motif | 6 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 23 – 48 | Polar residuesSequence analysisAdd BLAST | 26 | |
Compositional biasi | 49 – 67 | Pro residuesSequence analysisAdd BLAST | 19 | |
Compositional biasi | 68 – 107 | Polar residuesSequence analysisAdd BLAST | 40 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3811, Eukaryota |
InParanoidi | P34056 |
PhylomeDBi | P34056 |
TreeFami | TF313718 |
Family and domain databases
InterProi | View protein in InterPro IPR004979, TF_AP2 IPR008121, TF_AP2_alpha_N IPR013854, TF_AP2_C |
PANTHERi | PTHR10812, PTHR10812, 1 hit |
Pfami | View protein in Pfam PF03299, TF_AP-2, 1 hit |
PRINTSi | PR01749, AP2ATNSCPFCT PR01748, AP2TNSCPFCT |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MLWKLTDNIK YEDCEDRHDG TSNGTARLPQ LGTVGQSPYT SAPPLSHTPN
60 70 80 90 100
ADFQPPYFPP PYQPIYPQSQ DPYSHVNDPY SLNPLHAQPQ PQHPGWPGQR
110 120 130 140 150
QSQESGLLHT HRGLPHQLSG LDPRRDYRRH EDLLHGPHAL GSGLGDLPIH
160 170 180 190 200
SLPHAIEDVP HVEDPGINIP DQTVIKKGPV SLSKSNSNAV SAIPINKDNL
210 220 230 240 250
FGGVVNPNEV FCSVPGRLSL LSSTSKYKVT VAEVQRRLSP PECLNASLLG
260 270 280 290 300
GVLRRAKSKN GGRSLREKLD KIGLNLPAGR RKAANVTLLT SLVEGEAVHL
310 320 330 340 350
ARDFGYVCET EFPAKAVAEF LNRQHSDPNE QVARKNMLLA TKQICKEFTD
360 370 380 390 400
LLAQDRSPLG NSRPNPILEP GIQSCLTHFN LISHGFGSPA VCAAVTALQN
410 420 430
YLTEALKAMD KMYLSNNPNS HTDNSAKSSD KEEKHRK
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ8BPN4 | Q8BPN4_MOUSE | AP-2 transcription factor | Tfap2a Tcfap2a | 431 | Annotation score: | ||
A0A0R4J1E6 | A0A0R4J1E6_MOUSE | AP-2 transcription factor | Tfap2a | 437 | Annotation score: | ||
A0A286YD43 | A0A286YD43_MOUSE | AP-2 transcription factor | Tfap2a | 466 | Annotation score: | ||
A0A286YCU0 | A0A286YCU0_MOUSE | AP-2 transcription factor | Tfap2a | 433 | Annotation score: | ||
A0A286YDR3 | A0A286YDR3_MOUSE | AP-2 transcription factor | Tfap2a | 439 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 38 | P → S in AAH07471 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 139 | A → G (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 139 | A → G (PubMed:7750631).Curated | 1 | |
Sequence conflicti | 158 – 159 | DV → RL (PubMed:16141072).Curated | 2 | |
Sequence conflicti | 390 | Missing (PubMed:1989904).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_006402 | 1 – 15 | MLWKL…YEDCE → MLVHSFSAM in isoform 3. CuratedAdd BLAST | 15 | |
Alternative sequenceiVSP_006403 | 1 – 15 | MLWKL…YEDCE → MNSVVVDTPYFGGLPTLGLW NGFSRVVEALRLISSSPSRL AFFQ in isoform 4. CuratedAdd BLAST | 15 | |
Alternative sequenceiVSP_006404 | 16 – 160 | Missing in isoform 2. CuratedAdd BLAST | 145 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X74216 mRNA Translation: CAA52292.1 BC018226 mRNA Translation: AAH18226.1 BC007471 mRNA Translation: AAH07471.1 U17285 mRNA Translation: AAA85681.1 U17291, U17289 Genomic DNA Translation: AAA85678.1 U17286 mRNA Translation: AAA85682.1 U17287 mRNA Translation: AAA85683.1 U17291, U17290 Genomic DNA Translation: AAA85679.1 U17288 mRNA Translation: AAA85684.1 U17291 Genomic DNA Translation: AAA85680.1 AK013900 mRNA Translation: BAB29047.1 X57012 mRNA Translation: CAA40331.1 |
CCDSi | CCDS49243.1 [P34056-3] |
PIRi | S42111 |
RefSeqi | NP_001288603.1, NM_001301674.1 NP_035677.2, NM_011547.4 |
Genome annotation databases
GeneIDi | 21418 |
KEGGi | mmu:21418 |
UCSCi | uc007qei.1, mouse [P34056-4] uc011yyq.1, mouse [P34056-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X74216 mRNA Translation: CAA52292.1 BC018226 mRNA Translation: AAH18226.1 BC007471 mRNA Translation: AAH07471.1 U17285 mRNA Translation: AAA85681.1 U17291, U17289 Genomic DNA Translation: AAA85678.1 U17286 mRNA Translation: AAA85682.1 U17287 mRNA Translation: AAA85683.1 U17291, U17290 Genomic DNA Translation: AAA85679.1 U17288 mRNA Translation: AAA85684.1 U17291 Genomic DNA Translation: AAA85680.1 AK013900 mRNA Translation: BAB29047.1 X57012 mRNA Translation: CAA40331.1 |
CCDSi | CCDS49243.1 [P34056-3] |
PIRi | S42111 |
RefSeqi | NP_001288603.1, NM_001301674.1 NP_035677.2, NM_011547.4 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 204011, 7 interactors |
CORUMi | P34056 |
IntActi | P34056, 1 interactor |
STRINGi | 10090.ENSMUSP00000105822 |
PTM databases
iPTMneti | P34056 |
PhosphoSitePlusi | P34056 |
Proteomic databases
MaxQBi | P34056 |
PaxDbi | P34056 |
PeptideAtlasi | P34056 |
PRIDEi | P34056 |
ProteomicsDBi | 296054 [P34056-1] 296055 [P34056-2] 296056 [P34056-3] 296057 [P34056-4] |
Protocols and materials databases
DNASUi | 21418 |
Genome annotation databases
GeneIDi | 21418 |
KEGGi | mmu:21418 |
UCSCi | uc007qei.1, mouse [P34056-4] uc011yyq.1, mouse [P34056-2] |
Organism-specific databases
CTDi | 7020 |
MGIi | MGI:104671, Tfap2a |
Phylogenomic databases
eggNOGi | KOG3811, Eukaryota |
InParanoidi | P34056 |
PhylomeDBi | P34056 |
TreeFami | TF313718 |
Enzyme and pathway databases
Reactomei | R-MMU-8864260, Transcriptional regulation by the AP-2 (TFAP2) family of transcription factors R-MMU-8866904, Negative regulation of activity of TFAP2 (AP-2) family transcription factors R-MMU-8866907, Activation of the TFAP2 (AP-2) family of transcription factors R-MMU-8869496, TFAP2A acts as a transcriptional repressor during retinoic acid induced cell differentiation |
Miscellaneous databases
BioGRID-ORCSi | 21418, 4 hits in 62 CRISPR screens |
ChiTaRSi | Tfap2a, mouse |
PROi | PR:P34056 |
RNActi | P34056, protein |
SOURCEi | Search... |
Family and domain databases
InterProi | View protein in InterPro IPR004979, TF_AP2 IPR008121, TF_AP2_alpha_N IPR013854, TF_AP2_C |
PANTHERi | PTHR10812, PTHR10812, 1 hit |
Pfami | View protein in Pfam PF03299, TF_AP-2, 1 hit |
PRINTSi | PR01749, AP2ATNSCPFCT PR01748, AP2TNSCPFCT |
MobiDBi | Search... |
Entry informationi
Entry namei | AP2A_MOUSE | |
Accessioni | P34056Primary (citable) accession number: P34056 Secondary accession number(s): Q60740 Q9CRY4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | November 1, 1995 | |
Last modified: | February 23, 2022 | |
This is version 183 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families