UniProtKB - P34047 (HIS5A_ARATH)
Protein
Imidazoleglycerol-phosphate dehydratase 1, chloroplastic
Gene
HISN5A
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Catalytic activityi
- D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O1 PublicationEC:4.2.1.191 Publication
Cofactori
Mn2+1 PublicationNote: Binds 2 manganese ions per subunit.1 Publication
: L-histidine biosynthesis Pathwayi
This protein is involved in step 6 of the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate.1 PublicationProteins known to be involved in the 9 steps of the subpathway in this organism are:
- ATP phosphoribosyltransferase (AXX17_At1g09770), ATP phosphoribosyltransferase (AN1_LOCUS1062), ATP phosphoribosyltransferase (AN1_LOCUS4869), ATP phosphoribosyltransferase (C24_LOCUS4752), ATP phosphoribosyltransferase 2, chloroplastic (HISN1B), ATP phosphoribosyltransferase (C24_LOCUS976), ATP phosphoribosyltransferase 1, chloroplastic (HISN1A), ATP phosphoribosyltransferase (AXX17_At1g52040)
- Phosphoribosyl-AMP cyclohydrolase (AN1_LOCUS3233), Phosphoribosyl-AMP cyclohydrolase (C24_LOCUS3145), Phosphoribosyl-ATP diphosphatase (At1g31860), Histidine biosynthesis bifunctional protein hisIE, chloroplastic (HISN2), Phosphoribosyl-AMP cyclohydrolase (At1g31860), Phosphoribosyl-AMP cyclohydrolase (AT-IE)
- Phosphoribosyl-AMP cyclohydrolase (AT-IE), Phosphoribosyl-AMP cyclohydrolase (AN1_LOCUS3233), Phosphoribosyl-AMP cyclohydrolase (C24_LOCUS3145), Histidine biosynthesis bifunctional protein hisIE, chloroplastic (HISN2), Phosphoribosyl-AMP cyclohydrolase (At1g31860), Phosphoribosyl-AMP cyclohydrolase (AT-IE)
- 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase, chloroplastic (At2g36230), 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase, chloroplastic, 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase, chloroplastic (AN1_LOCUS10075), 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase, chloroplastic (AXX17_At2g32880), 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase, chloroplastic (HISN3)
- Imidazole glycerol phosphate synthase hisHF (AXX17_At4g31050), Imidazole glycerol phosphate synthase hisHF, chloroplastic (HISN4), AT4G26900 protein (At4g26900), Imidazole glycerol phosphate synthase hisHF (AN1_LOCUS19446)
- Imidazoleglycerol-phosphate dehydratase 2, chloroplastic (HISN5B), Imidazoleglycerol-phosphate dehydratase (AXX17_At4g17260), Imidazoleglycerol-phosphate dehydratase (HISN5B), Imidazoleglycerol-phosphate dehydratase (AXX17_At3g24180), Imidazoleglycerol-phosphate dehydratase (At3g22425), Imidazoleglycerol-phosphate dehydratase 1, chloroplastic (HISN5A), Imidazoleglycerol-phosphate dehydratase (HISN5B), Imidazoleglycerol-phosphate dehydratase (At4g14910)
- Histidinol-phosphate aminotransferase 2, chloroplastic (HISN6B), Histidinol-phosphate aminotransferase 1, chloroplastic (HISN6A)
- Bifunctional phosphatase IMPL2, chloroplastic (HISN7), Histidinol-phosphatase (AXX17_At4g44500)
- Histidinol dehydrogenase, chloroplastic (AXX17_At5g63470), Histidinol dehydrogenase, chloroplastic (HISN8)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway L-histidine biosynthesis and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 84 | SubstrateBy similarity | 1 | |
Metal bindingi | 110 | Manganese 1; via tele nitrogenCombined sources1 Publication | 1 | |
Metal bindingi | 136 | Manganese 2; via tele nitrogenCombined sources1 Publication | 1 | |
Metal bindingi | 137 | Manganese 1; via tele nitrogenCombined sources1 Publication | 1 | |
Metal bindingi | 140 | Manganese 2Combined sources1 Publication | 1 | |
Binding sitei | 162 | SubstrateBy similarity | 1 | |
Binding sitei | 184 | SubstrateBy similarity | 1 | |
Metal bindingi | 208 | Manganese 2; via tele nitrogenCombined sources1 Publication | 1 | |
Metal bindingi | 232 | Manganese 1; via tele nitrogenCombined sources1 Publication | 1 | |
Metal bindingi | 233 | Manganese 2; via tele nitrogenCombined sources1 Publication | 1 | |
Metal bindingi | 236 | Manganese 1Combined sources1 Publication | 1 |
GO - Molecular functioni
- imidazoleglycerol-phosphate dehydratase activity Source: TAIR
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- histidine biosynthetic process Source: TAIR
Keywordsi
Molecular function | Lyase |
Biological process | Amino-acid biosynthesis, Histidine biosynthesis |
Ligand | Manganese, Metal-binding |
Enzyme and pathway databases
BioCyci | MetaCyc:AT3G22425-MONOMER |
UniPathwayi | UPA00031;UER00011 |
Names & Taxonomyi
Protein namesi | Recommended name: Imidazoleglycerol-phosphate dehydratase 1, chloroplastic1 Publication (EC:4.2.1.191 Publication)Short name: IGPD 11 Publication Alternative name(s): Protein HISTIDINE BIOSYNTHESIS 5A1 Publication |
Gene namesi | Name:HISN5A1 Publication Ordered Locus Names:At3g22425Imported ORF Names:MCB17.17Imported |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT3G22425 |
TAIRi | locus:1005716545, AT3G22425 |
Subcellular locationi
Chloroplast
- chloroplast Curated
Chloroplast
- chloroplast Source: UniProtKB-SubCell
Keywords - Cellular componenti
Chloroplast, PlastidPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 62 | ChloroplastCombined sourcesAdd BLAST | 62 | |
ChainiPRO_0000158253 | 63 – 270 | Imidazoleglycerol-phosphate dehydratase 1, chloroplasticAdd BLAST | 208 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 63 | N-acetylserineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
PaxDbi | P34047 |
PRIDEi | P34047 |
ProteomicsDBi | 230245 [P34047-1] |
PTM databases
iPTMneti | P34047 |
Expressioni
Gene expression databases
ExpressionAtlasi | P34047, baseline and differential |
Genevisiblei | P34047, AT |
Interactioni
Protein-protein interaction databases
BioGRIDi | 7144, 1 interactor |
DIPi | DIP-48462N |
STRINGi | 3702.AT3G22425.2 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P34047 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P34047 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 110 – 118 | Substrate bindingBy similarity | 9 | |
Regioni | 136 – 140 | Substrate bindingBy similarity | 5 | |
Regioni | 232 – 240 | Substrate bindingBy similarity | 9 | |
Regioni | 262 – 264 | Substrate bindingBy similarity | 3 |
Sequence similaritiesi
Belongs to the imidazoleglycerol-phosphate dehydratase family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG3143, Eukaryota |
HOGENOMi | CLU_044308_1_1_1 |
OMAi | ARHGLFD |
PhylomeDBi | P34047 |
Family and domain databases
CDDi | cd07914, IGPD, 1 hit |
Gene3Di | 3.30.230.40, 2 hits |
HAMAPi | MF_00076, HisB, 1 hit |
InterProi | View protein in InterPro IPR038494, IGPD_sf IPR000807, ImidazoleglycerolP_deHydtase IPR020565, ImidazoleglycerP_deHydtase_CS IPR020568, Ribosomal_S5_D2-typ_fold |
PANTHERi | PTHR23133, PTHR23133, 1 hit |
Pfami | View protein in Pfam PF00475, IGPD, 1 hit |
SUPFAMi | SSF54211, SSF54211, 2 hits |
PROSITEi | View protein in PROSITE PS00954, IGP_DEHYDRATASE_1, 1 hit PS00955, IGP_DEHYDRATASE_2, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketNote: Experimental confirmation may be lacking for some isoforms.
Isoform 1 (identifier: P34047-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MELSSASAIL SHSSSAAQLL RPKLGFIDLL PRRAMIVSSP SSSLPRFLRM
60 70 80 90 100
ESQSQLRQSI SCSASSSSSM ALGRIGEVKR VTKETNVSVK INLDGTGVAD
110 120 130 140 150
SSSGIPFLDH MLDQLASHGL FDVHVRATGD VHIDDHHTNE DIALAIGTAL
160 170 180 190 200
LKALGERKGI NRFGDFTAPL DEALIHVSLD LSGRPYLGYN LEIPTQRVGT
210 220 230 240 250
YDTQLVEHFF QSLVNTSGMT LHIRQLAGEN SHHIIEATFK AFARALRQAT
260 270
ETDPRRGGTI PSSKGVLSRS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 201 | Y → C in BAD44082 (Ref. 6) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_008895 | 205 – 270 | LVEHF…VLSRS → VLSLLLELSSFGFICVIRCL VIIESVAKNCLTFRFVVGGA LFPVVGEYFWYDSSHPAARW in isoform 2. 1 PublicationAdd BLAST | 66 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U02689 mRNA Translation: AAA93196.1 AB022215 Genomic DNA Translation: BAB01781.1 CP002686 Genomic DNA Translation: AEE76636.1 CP002686 Genomic DNA Translation: AEE76637.1 AK118815 mRNA Translation: BAC43405.1 AY070442 mRNA Translation: AAL49845.1 AK176319 mRNA Translation: BAD44082.1 AK176429 mRNA Translation: BAD44192.1 AY087948 mRNA Translation: AAM65496.1 |
RefSeqi | NP_850624.1, NM_180293.1 [P34047-2] NP_850625.1, NM_180294.2 [P34047-1] |
Genome annotation databases
EnsemblPlantsi | AT3G22425.1; AT3G22425.1; AT3G22425 [P34047-2] AT3G22425.2; AT3G22425.2; AT3G22425 [P34047-1] |
GeneIDi | 821812 |
Gramenei | AT3G22425.1; AT3G22425.1; AT3G22425 [P34047-2] AT3G22425.2; AT3G22425.2; AT3G22425 [P34047-1] |
KEGGi | ath:AT3G22425 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U02689 mRNA Translation: AAA93196.1 AB022215 Genomic DNA Translation: BAB01781.1 CP002686 Genomic DNA Translation: AEE76636.1 CP002686 Genomic DNA Translation: AEE76637.1 AK118815 mRNA Translation: BAC43405.1 AY070442 mRNA Translation: AAL49845.1 AK176319 mRNA Translation: BAD44082.1 AK176429 mRNA Translation: BAD44192.1 AY087948 mRNA Translation: AAM65496.1 |
RefSeqi | NP_850624.1, NM_180293.1 [P34047-2] NP_850625.1, NM_180294.2 [P34047-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2F1D | X-ray | 3.00 | A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P | 64-270 | [»] | |
SMRi | P34047 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 7144, 1 interactor |
DIPi | DIP-48462N |
STRINGi | 3702.AT3G22425.2 |
PTM databases
iPTMneti | P34047 |
Proteomic databases
PaxDbi | P34047 |
PRIDEi | P34047 |
ProteomicsDBi | 230245 [P34047-1] |
Genome annotation databases
EnsemblPlantsi | AT3G22425.1; AT3G22425.1; AT3G22425 [P34047-2] AT3G22425.2; AT3G22425.2; AT3G22425 [P34047-1] |
GeneIDi | 821812 |
Gramenei | AT3G22425.1; AT3G22425.1; AT3G22425 [P34047-2] AT3G22425.2; AT3G22425.2; AT3G22425 [P34047-1] |
KEGGi | ath:AT3G22425 |
Organism-specific databases
Araporti | AT3G22425 |
TAIRi | locus:1005716545, AT3G22425 |
Phylogenomic databases
eggNOGi | KOG3143, Eukaryota |
HOGENOMi | CLU_044308_1_1_1 |
OMAi | ARHGLFD |
PhylomeDBi | P34047 |
Enzyme and pathway databases
UniPathwayi | UPA00031;UER00011 |
BioCyci | MetaCyc:AT3G22425-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P34047 |
PROi | PR:P34047 |
Gene expression databases
ExpressionAtlasi | P34047, baseline and differential |
Genevisiblei | P34047, AT |
Family and domain databases
CDDi | cd07914, IGPD, 1 hit |
Gene3Di | 3.30.230.40, 2 hits |
HAMAPi | MF_00076, HisB, 1 hit |
InterProi | View protein in InterPro IPR038494, IGPD_sf IPR000807, ImidazoleglycerolP_deHydtase IPR020565, ImidazoleglycerP_deHydtase_CS IPR020568, Ribosomal_S5_D2-typ_fold |
PANTHERi | PTHR23133, PTHR23133, 1 hit |
Pfami | View protein in Pfam PF00475, IGPD, 1 hit |
SUPFAMi | SSF54211, SSF54211, 2 hits |
PROSITEi | View protein in PROSITE PS00954, IGP_DEHYDRATASE_1, 1 hit PS00955, IGP_DEHYDRATASE_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HIS5A_ARATH | |
Accessioni | P34047Primary (citable) accession number: P34047 Secondary accession number(s): Q67YN9, Q67YZ9, Q8VYM1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | February 1, 1994 | |
Last modified: | December 2, 2020 | |
This is version 155 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families