UniProtKB - P34021 (ECR_DROME)
Ecdysone receptor
EcR
Functioni
Receptor for ecdysone (PubMed:1913820, PubMed:30293839).
Binds to ecdysone response elements (ECRES) following ecdysone-binding, and recruitment of a complex containing the histone methyltransferase trr, leads to activate transcription of target genes (PubMed:1913820, PubMed:30293839).
2 PublicationsRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 264 – 336 | Nuclear receptorPROSITE-ProRule annotationAdd BLAST | 73 | |
Zinc fingeri | 264 – 284 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 21 | |
Zinc fingeri | 300 – 324 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 25 |
GO - Molecular functioni
- core promoter sequence-specific DNA binding Source: CAFA
- DNA binding Source: FlyBase
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: FlyBase
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: FlyBase
- DNA-binding transcription factor binding Source: FlyBase
- ecdysone binding Source: FlyBase
- nuclear receptor activity Source: FlyBase
- protein heterodimerization activity Source: CAFA
- protein homodimerization activity Source: CAFA
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: FlyBase
- signaling receptor binding Source: CAFA
- steroid binding Source: FlyBase
- transcription cis-regulatory region binding Source: FlyBase
- transcription coactivator binding Source: FlyBase
- transcription corepressor binding Source: FlyBase
- zinc ion binding Source: InterPro
GO - Biological processi
- autophagy Source: FlyBase
- border follicle cell migration Source: FlyBase
- cardiac muscle tissue development Source: FlyBase
- cardioblast differentiation Source: FlyBase
- cell adhesion Source: FlyBase
- cell differentiation Source: GO_Central
- chitin-based cuticle development Source: FlyBase
- chitin-based embryonic cuticle biosynthetic process Source: FlyBase
- cholesterol homeostasis Source: FlyBase
- dendrite morphogenesis Source: FlyBase
- determination of adult lifespan Source: FlyBase
- dorsal vessel heart proper cell fate commitment Source: FlyBase
- ecdysis, chitin-based cuticle Source: FlyBase
- ecdysone-mediated induction of salivary gland cell autophagic cell death Source: FlyBase
- ecdysone receptor-mediated signaling pathway Source: UniProtKB
- epidermis development Source: FlyBase
- germ-band shortening Source: FlyBase
- germ cell development Source: FlyBase
- hatching Source: FlyBase
- head involution Source: FlyBase
- histoblast morphogenesis Source: FlyBase
- imaginal disc-derived wing morphogenesis Source: FlyBase
- larval central nervous system remodeling Source: FlyBase
- larval development Source: UniProtKB
- larval wandering behavior Source: FlyBase
- long-term memory Source: FlyBase
- Malpighian tubule morphogenesis Source: FlyBase
- metamorphosis Source: FlyBase
- mushroom body development Source: FlyBase
- negative regulation of transcription, DNA-templated Source: FlyBase
- negative regulation of transcription by RNA polymerase II Source: GO_Central
- neuron remodeling Source: FlyBase
- oogenesis Source: FlyBase
- peripheral nervous system development Source: FlyBase
- phagocytosis, engulfment Source: FlyBase
- positive regulation of apoptotic process Source: FlyBase
- positive regulation of circadian sleep/wake cycle, sleep Source: FlyBase
- positive regulation of neuron remodeling Source: FlyBase
- positive regulation of transcription, DNA-templated Source: FlyBase
- positive regulation of transcription by RNA polymerase II Source: FlyBase
- pupariation Source: FlyBase
- regulation of autophagy Source: FlyBase
- regulation of cellular respiration Source: FlyBase
- regulation of development, heterochronic Source: FlyBase
- regulation of hemocyte proliferation Source: FlyBase
- regulation of Malpighian tubule diameter Source: FlyBase
- regulation of neuron remodeling Source: FlyBase
- regulation of transcription by RNA polymerase II Source: FlyBase
- response to cocaine Source: FlyBase
- response to ecdysone Source: FlyBase
- salivary gland cell autophagic cell death Source: FlyBase
- sperm individualization Source: FlyBase
Keywordsi
Molecular function | DNA-binding, Receptor |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-DME-159418, Recycling of bile acids and salts R-DME-196791, Vitamin D (calciferol) metabolism R-DME-383280, Nuclear Receptor transcription pathway R-DME-4090294, SUMOylation of intracellular receptors R-DME-9029569, NR1H3 & NR1H2 regulate gene expression linked to cholesterol transport and efflux R-DME-9623433, NR1H2 & NR1H3 regulate gene expression to control bile acid homeostasis |
SignaLinki | P34021 |
Names & Taxonomyi
Protein namesi | Recommended name: Ecdysone receptorAlternative name(s): 20-hydroxy-ecdysone receptor Short name: 20E receptor EcRH Ecdysteroid receptor Nuclear receptor subfamily 1 group H member 1 |
Gene namesi | Name:EcR Synonyms:NR1H1 ORF Names:CG1765 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0000546, EcR |
VEuPathDBi | VectorBase:FBgn0000546 |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation2 Publications
Nucleus
- activator ecdysone receptor complex Source: FlyBase
- ecdysone receptor holocomplex Source: CAFA
- nucleus Source: FlyBase
- repressor ecdysone receptor complex Source: FlyBase
Other locations
- cytoplasm Source: FlyBase
- dendrite Source: FlyBase
- polytene chromosome Source: UniProtKB
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
Chemistry databases
ChEMBLi | CHEMBL5676 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000053524 | 1 – 878 | Ecdysone receptorAdd BLAST | 878 |
Proteomic databases
PaxDbi | P34021 |
PRIDEi | P34021 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | FBgn0000546, Expressed in pupa and 35 other tissues |
ExpressionAtlasi | P34021, baseline and differential |
Genevisiblei | P34021, DM |
Interactioni
Subunit structurei
GO - Molecular functioni
- DNA-binding transcription factor binding Source: FlyBase
- protein heterodimerization activity Source: CAFA
- protein homodimerization activity Source: CAFA
- signaling receptor binding Source: CAFA
- transcription coactivator binding Source: FlyBase
- transcription corepressor binding Source: FlyBase
Protein-protein interaction databases
BioGRIDi | 61444, 120 interactors |
DIPi | DIP-158N |
IntActi | P34021, 38 interactors |
MINTi | P34021 |
STRINGi | 7227.FBpp0291631 |
Chemistry databases
BindingDBi | P34021 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P34021 |
SMRi | P34021 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P34021 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 419 – 654 | NR LBDPROSITE-ProRule annotationAdd BLAST | 236 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 263 | ModulatingAdd BLAST | 263 | |
Regioni | 1 – 27 | DisorderedSequence analysisAdd BLAST | 27 | |
Regioni | 209 – 254 | DisorderedSequence analysisAdd BLAST | 46 | |
Regioni | 344 – 374 | DisorderedSequence analysisAdd BLAST | 31 | |
Regioni | 698 – 759 | DisorderedSequence analysisAdd BLAST | 62 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 12 – 27 | Polar residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 215 – 245 | Polar residuesSequence analysisAdd BLAST | 31 | |
Compositional biasi | 344 – 358 | Basic and acidic residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 717 – 759 | Polar residuesSequence analysisAdd BLAST | 43 |
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 264 – 284 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 21 | |
Zinc fingeri | 300 – 324 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 25 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG3575, Eukaryota |
GeneTreei | ENSGT00940000173863 |
InParanoidi | P34021 |
OMAi | GMSLNTH |
PhylomeDBi | P34021 |
Family and domain databases
CDDi | cd06938, NR_LBD_EcR, 1 hit |
DisProti | DP01569 [P34021-2] |
Gene3Di | 1.10.565.10, 1 hit 3.30.50.10, 1 hit |
IDEALi | IID50251 |
InterProi | View protein in InterPro IPR003069, Ecdystd_rcpt IPR035500, NHR-like_dom_sf IPR041889, NR_LBD_EcR IPR000536, Nucl_hrmn_rcpt_lig-bd IPR001723, Nuclear_hrmn_rcpt IPR001628, Znf_hrmn_rcpt IPR013088, Znf_NHR/GATA |
Pfami | View protein in Pfam PF00104, Hormone_recep, 1 hit PF00105, zf-C4, 1 hit |
PRINTSi | PR01283, ECDYSTEROIDR PR00398, STRDHORMONER PR00047, STROIDFINGER |
SMARTi | View protein in SMART SM00430, HOLI, 1 hit SM00399, ZnF_C4, 1 hit |
SUPFAMi | SSF48508, SSF48508, 1 hit |
PROSITEi | View protein in PROSITE PS51843, NR_LBD, 1 hit PS00031, NUCLEAR_REC_DBD_1, 1 hit PS51030, NUCLEAR_REC_DBD_2, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MKRRWSNNGG FMRLPEESSS EVTSSSNGLV LPSGVNMSPS SLDSHDYCDQ
60 70 80 90 100
DLWLCGNESG SFGGSNGHGL SQQQQSVITL AMHGCSSTLP AQTTIIPING
110 120 130 140 150
NANGNGGSTN GQYVPGATNL GALANGMLNG GFNGMQQQIQ NGHGLINSTT
160 170 180 190 200
PSTPTTPLHL QQNLGGAGGG GIGGMGILHH ANGTPNGLIG VVGGGGGVGL
210 220 230 240 250
GVGGGGVGGL GMQHTPRSDS VNSISSGRDD LSPSSSLNGY SANESCDAKK
260 270 280 290 300
SKKGPAPRVQ EELCLVCGDR ASGYHYNALT CEGCKGFFRR SVTKSAVYCC
310 320 330 340 350
KFGRACEMDM YMRRKCQECR LKKCLAVGMR PECVVPENQC AMKRREKKAQ
360 370 380 390 400
KEKDKMTTSP SSQHGGNGSL ASGGGQDFVK KEILDLMTCE PPQHATIPLL
410 420 430 440 450
PDEILAKCQA RNIPSLTYNQ LAVIYKLIWY QDGYEQPSEE DLRRIMSQPD
460 470 480 490 500
ENESQTDVSF RHITEITILT VQLIVEFAKG LPAFTKIPQE DQITLLKACS
510 520 530 540 550
SEVMMLRMAR RYDHSSDSIF FANNRSYTRD SYKMAGMADN IEDLLHFCRQ
560 570 580 590 600
MFSMKVDNVE YALLTAIVIF SDRPGLEKAQ LVEAIQSYYI DTLRIYILNR
610 620 630 640 650
HCGDSMSLVF YAKLLSILTE LRTLGNQNAE MCFSLKLKNR KLPKFLEEIW
660 670 680 690 700
DVHAIPPSVQ SHLQITQEEN ERLERAERMR ASVGGAITAG IDCDSASTSA
710 720 730 740 750
AAAAAQHQPQ PQPQPQPSSL TQNDSQHQTQ PQLQPQLPPQ LQGQLQPQLQ
760 770 780 790 800
PQLQTQLQPQ IQPQPQLLPV SAPVPASVTA PGSLSAVSTS SEYMGGSAAI
810 820 830 840 850
GPITPATTSS ITAAVTASST TSAVPMGNGV GVGVGVGGNV SMYANAQTAM
860 870
ALMGVALHSH QEQLIGGVAV KSEHSTTA
The sequence of this isoform differs from the canonical sequence as follows:
1-226: MKRRWSNNGG...RSDSVNSISS → MLTTSGQQQS...YDPYSPTGKT
The sequence of this isoform differs from the canonical sequence as follows:
1-226: MKRRWSNNGG...RSDSVNSISS → MDTCGLVAELAHYIDAY
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE1JGY2 | E1JGY2_DROME | Ecdysone receptor | EcR anon-WO0229075.1, CG8347, DEcR, dECR, Dhr23 | 878 | Annotation score: | ||
A4UZ51 | A4UZ51_DROME | Ecdysone receptor | EcR anon-WO0229075.1, CG8347, DEcR, dECR, Dhr23 | 849 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 867 – 878 | GVAVK…HSTTA → EWRLSRSTRRLHSRRRVSST NITTTTSTSCWSRKRS in AAL68274 (PubMed:12537569).CuratedAdd BLAST | 12 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_003661 | 1 – 226 | MKRRW…NSISS → MLTTSGQQQSKQKLSTLPSH ILLQQQLAASAGPSSSVSLS PSSSAALTLHVASANGGARE TTSAAAVKDKLRPTPTAIKI EPMPDVISVGTVAGGSSVAT VVAPAATTTSNKPNSTAAPS TSAAAANGHLVLVPNKRPRL DVTEDWMSTPSPGSVPSSAP PLSPSPGSQNHSYNMSNGYA SPMSAGSYDPYSPTGKT in isoform ECR-A. 1 PublicationAdd BLAST | 226 | |
Alternative sequenceiVSP_003662 | 1 – 226 | MKRRW…NSISS → MDTCGLVAELAHYIDAY in isoform ECR-B2. CuratedAdd BLAST | 226 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M74078 mRNA Translation: AAA28498.1 S63761 mRNA Translation: AAB27496.2 AE013599 Genomic DNA Translation: AAF57278.3 AE013599 Genomic DNA Translation: AAF57280.2 AE013599 Genomic DNA Translation: AAM68347.1 AY075461 mRNA Translation: AAL68274.1 AY058575 mRNA Translation: AAL13804.1 Different initiation. BT012469 mRNA Translation: AAS93740.1 BT015234 mRNA Translation: AAT94463.1 |
PIRi | A40709 A41055 |
RefSeqi | NP_001163061.1, NM_001169590.2 [P34021-1] NP_724456.1, NM_165461.3 [P34021-2] NP_724457.1, NM_165462.2 [P34021-2] NP_724458.1, NM_165463.2 [P34021-2] NP_724459.1, NM_165464.3 [P34021-3] NP_724460.1, NM_165465.3 [P34021-1] |
Genome annotation databases
EnsemblMetazoai | FBtr0086008; FBpp0085349; FBgn0000546 [P34021-2] FBtr0086009; FBpp0085350; FBgn0000546 [P34021-2] FBtr0086010; FBpp0085351; FBgn0000546 [P34021-2] FBtr0086011; FBpp0085352; FBgn0000546 [P34021-1] FBtr0086012; FBpp0085353; FBgn0000546 [P34021-3] FBtr0302439; FBpp0291631; FBgn0000546 [P34021-1] |
GeneIDi | 35540 |
KEGGi | dme:Dmel_CG1765 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M74078 mRNA Translation: AAA28498.1 S63761 mRNA Translation: AAB27496.2 AE013599 Genomic DNA Translation: AAF57278.3 AE013599 Genomic DNA Translation: AAF57280.2 AE013599 Genomic DNA Translation: AAM68347.1 AY075461 mRNA Translation: AAL68274.1 AY058575 mRNA Translation: AAL13804.1 Different initiation. BT012469 mRNA Translation: AAS93740.1 BT015234 mRNA Translation: AAT94463.1 |
PIRi | A40709 A41055 |
RefSeqi | NP_001163061.1, NM_001169590.2 [P34021-1] NP_724456.1, NM_165461.3 [P34021-2] NP_724457.1, NM_165462.2 [P34021-2] NP_724458.1, NM_165463.2 [P34021-2] NP_724459.1, NM_165464.3 [P34021-3] NP_724460.1, NM_165465.3 [P34021-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1R0N | X-ray | 2.60 | B | 256-364 | [»] | |
1R0O | X-ray | 2.24 | B | 256-364 | [»] | |
2HAN | X-ray | 1.95 | B | 256-365 | [»] | |
AlphaFoldDBi | P34021 | |||||
SMRi | P34021 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 61444, 120 interactors |
DIPi | DIP-158N |
IntActi | P34021, 38 interactors |
MINTi | P34021 |
STRINGi | 7227.FBpp0291631 |
Chemistry databases
BindingDBi | P34021 |
ChEMBLi | CHEMBL5676 |
Proteomic databases
PaxDbi | P34021 |
PRIDEi | P34021 |
Genome annotation databases
EnsemblMetazoai | FBtr0086008; FBpp0085349; FBgn0000546 [P34021-2] FBtr0086009; FBpp0085350; FBgn0000546 [P34021-2] FBtr0086010; FBpp0085351; FBgn0000546 [P34021-2] FBtr0086011; FBpp0085352; FBgn0000546 [P34021-1] FBtr0086012; FBpp0085353; FBgn0000546 [P34021-3] FBtr0302439; FBpp0291631; FBgn0000546 [P34021-1] |
GeneIDi | 35540 |
KEGGi | dme:Dmel_CG1765 |
Organism-specific databases
CTDi | 35540 |
FlyBasei | FBgn0000546, EcR |
VEuPathDBi | VectorBase:FBgn0000546 |
Phylogenomic databases
eggNOGi | KOG3575, Eukaryota |
GeneTreei | ENSGT00940000173863 |
InParanoidi | P34021 |
OMAi | GMSLNTH |
PhylomeDBi | P34021 |
Enzyme and pathway databases
Reactomei | R-DME-159418, Recycling of bile acids and salts R-DME-196791, Vitamin D (calciferol) metabolism R-DME-383280, Nuclear Receptor transcription pathway R-DME-4090294, SUMOylation of intracellular receptors R-DME-9029569, NR1H3 & NR1H2 regulate gene expression linked to cholesterol transport and efflux R-DME-9623433, NR1H2 & NR1H3 regulate gene expression to control bile acid homeostasis |
SignaLinki | P34021 |
Miscellaneous databases
BioGRID-ORCSi | 35540, 1 hit in 3 CRISPR screens |
EvolutionaryTracei | P34021 |
GenomeRNAii | 35540 |
PROi | PR:P34021 |
Gene expression databases
Bgeei | FBgn0000546, Expressed in pupa and 35 other tissues |
ExpressionAtlasi | P34021, baseline and differential |
Genevisiblei | P34021, DM |
Family and domain databases
CDDi | cd06938, NR_LBD_EcR, 1 hit |
DisProti | DP01569 [P34021-2] |
Gene3Di | 1.10.565.10, 1 hit 3.30.50.10, 1 hit |
IDEALi | IID50251 |
InterProi | View protein in InterPro IPR003069, Ecdystd_rcpt IPR035500, NHR-like_dom_sf IPR041889, NR_LBD_EcR IPR000536, Nucl_hrmn_rcpt_lig-bd IPR001723, Nuclear_hrmn_rcpt IPR001628, Znf_hrmn_rcpt IPR013088, Znf_NHR/GATA |
Pfami | View protein in Pfam PF00104, Hormone_recep, 1 hit PF00105, zf-C4, 1 hit |
PRINTSi | PR01283, ECDYSTEROIDR PR00398, STRDHORMONER PR00047, STROIDFINGER |
SMARTi | View protein in SMART SM00430, HOLI, 1 hit SM00399, ZnF_C4, 1 hit |
SUPFAMi | SSF48508, SSF48508, 1 hit |
PROSITEi | View protein in PROSITE PS51843, NR_LBD, 1 hit PS00031, NUCLEAR_REC_DBD_1, 1 hit PS51030, NUCLEAR_REC_DBD_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ECR_DROME | |
Accessioni | P34021Primary (citable) accession number: P34021 Secondary accession number(s): Q0E9N8 Q9V9K8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | February 1, 1994 | |
Last modified: | May 25, 2022 | |
This is version 232 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families