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Protein

DNA replication licensing factor MCM7

Gene

MCM7

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.3 Publications

Miscellaneous

Early fractionation of eukaryotic MCM proteins yielded a variety of dimeric, trimeric and tetrameric complexes with unclear biological significance. Specifically a MCM467 subcomplex is shown to have in vitro helicase activity which is inhibited by the MCM2 subunit. The MCM2-7 hexamer is the proposed physiological active complex.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi381 – 388ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processCell cycle, DNA replication
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-176187 Activation of ATR in response to replication stress
R-HSA-176974 Unwinding of DNA
R-HSA-68867 Assembly of the pre-replicative complex
R-HSA-68949 Orc1 removal from chromatin
R-HSA-68962 Activation of the pre-replicative complex
R-HSA-69052 Switching of origins to a post-replicative state
SIGNORiP33993

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor MCM7 (EC:3.6.4.12)
Alternative name(s):
CDC47 homolog
P1.1-MCM3
Gene namesi
Name:MCM7
Synonyms:CDC47, MCM2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

EuPathDBiHostDB:ENSG00000166508.17
HGNCiHGNC:6950 MCM7
MIMi600592 gene
neXtProtiNX_P33993

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi4176
OpenTargetsiENSG00000166508
PharmGKBiPA30697

Polymorphism and mutation databases

DMDMi20981696

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00001941192 – 719DNA replication licensing factor MCM7Add BLAST718

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1 Publication1
Cross-linki15Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki28Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei121PhosphoserineCombined sources1
Modified residuei314PhosphoserineCombined sources1
Modified residuei365PhosphoserineCombined sources1
Modified residuei500PhosphoserineCombined sources1
Modified residuei678PhosphoserineCombined sources1

Post-translational modificationi

O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.1 Publication

Keywords - PTMi

Acetylation, Glycoprotein, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP33993
MaxQBiP33993
PaxDbiP33993
PeptideAtlasiP33993
PRIDEiP33993
ProteomicsDBi54936
54937 [P33993-2]

PTM databases

iPTMnetiP33993
PhosphoSitePlusiP33993
SwissPalmiP33993

Expressioni

Gene expression databases

BgeeiENSG00000166508 Expressed in 196 organ(s), highest expression level in embryo
CleanExiHS_MCM2
HS_MCM7
ExpressionAtlasiP33993 baseline and differential
GenevisibleiP33993 HS

Organism-specific databases

HPAiCAB002163
CAB016312
HPA003898

Interactioni

Subunit structurei

Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5 (Probable). Interacts with the ATR-ATRIP complex and with RAD17. Interacts with TIPIN. Interacts with MCMBP. Interacts with ANKRD17. Component of the replisome complex composed of at least DONSON, MCM2, MCM7, PCNA and TICRR (PubMed:28191891).Curated8 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi110344, 175 interactors
ComplexPortaliCPX-2940 MCM complex
CORUMiP33993
DIPiDIP-27580N
IntActiP33993, 184 interactors
MINTiP33993
STRINGi9606.ENSP00000307288

Structurei

3D structure databases

ProteinModelPortaliP33993
SMRiP33993
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini332 – 538MCMAdd BLAST207

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni521 – 564Interaction with RAD171 PublicationAdd BLAST44
Regioni577 – 719Interaction with ATRIPAdd BLAST143

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi513 – 516Arginine finger4

Sequence similaritiesi

Belongs to the MCM family.Curated

Phylogenomic databases

eggNOGiKOG0482 Eukaryota
COG1241 LUCA
GeneTreeiENSGT00930000151047
HOGENOMiHOG000224125
HOVERGENiHBG000741
InParanoidiP33993
KOiK02210
OMAiVKIQEMA
OrthoDBiEOG091G02LB
PhylomeDBiP33993
TreeFamiTF300400

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR031327 MCM
IPR008050 MCM7
IPR018525 MCM_CS
IPR001208 MCM_dom
IPR027925 MCM_N
IPR033762 MCM_OB
IPR012340 NA-bd_OB-fold
IPR027417 P-loop_NTPase
PANTHERiPTHR11630 PTHR11630, 1 hit
PTHR11630:SF26 PTHR11630:SF26, 1 hit
PfamiView protein in Pfam
PF00493 MCM, 1 hit
PF14551 MCM_N, 1 hit
PF17207 MCM_OB, 1 hit
PRINTSiPR01657 MCMFAMILY
PR01663 MCMPROTEIN7
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SM00350 MCM, 1 hit
SUPFAMiSSF50249 SSF50249, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00847 MCM_1, 1 hit
PS50051 MCM_2, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P33993-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MALKDYALEK EKVKKFLQEF YQDDELGKKQ FKYGNQLVRL AHREQVALYV
60 70 80 90 100
DLDDVAEDDP ELVDSICENA RRYAKLFADA VQELLPQYKE REVVNKDVLD
110 120 130 140 150
VYIEHRLMME QRSRDPGMVR SPQNQYPAEL MRRFELYFQG PSSNKPRVIR
160 170 180 190 200
EVRADSVGKL VTVRGIVTRV SEVKPKMVVA TYTCDQCGAE TYQPIQSPTF
210 220 230 240 250
MPLIMCPSQE CQTNRSGGRL YLQTRGSRFI KFQEMKMQEH SDQVPVGNIP
260 270 280 290 300
RSITVLVEGE NTRIAQPGDH VSVTGIFLPI LRTGFRQVVQ GLLSETYLEA
310 320 330 340 350
HRIVKMNKSE DDESGAGELT REELRQIAEE DFYEKLAASI APEIYGHEDV
360 370 380 390 400
KKALLLLLVG GVDQSPRGMK IRGNINICLM GDPGVAKSQL LSYIDRLAPR
410 420 430 440 450
SQYTTGRGSS GVGLTAAVLR DSVSGELTLE GGALVLADQG VCCIDEFDKM
460 470 480 490 500
AEADRTAIHE VMEQQTISIA KAGILTTLNA RCSILAAANP AYGRYNPRRS
510 520 530 540 550
LEQNIQLPAA LLSRFDLLWL IQDRPDRDND LRLAQHITYV HQHSRQPPSQ
560 570 580 590 600
FEPLDMKLMR RYIAMCREKQ PMVPESLADY ITAAYVEMRR EAWASKDATY
610 620 630 640 650
TSARTLLAIL RLSTALARLR MVDVVEKEDV NEAIRLMEMS KDSLLGDKGQ
660 670 680 690 700
TARTQRPADV IFATVRELVS GGRSVRFSEA EQRCVSRGFT PAQFQAALDE
710
YEELNVWQVN ASRTRITFV
Length:719
Mass (Da):81,308
Last modified:May 15, 2002 - v4
Checksum:i330A1DEFAEFBFB88
GO
Isoform 2 (identifier: P33993-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     329-658: Missing.

Note: No experimental confirmation available.
Show »
Length:389
Mass (Da):44,649
Checksum:iCCAA37CB2DA54063
GO
Isoform 3 (identifier: P33993-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-176: Missing.

Show »
Length:543
Mass (Da):60,643
Checksum:i4CAA41B4F392F50C
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9J8M6C9J8M6_HUMAN
DNA replication licensing factor MC...
MCM7
183Annotation score:
H0YH42H0YH42_HUMAN
DNA replication licensing factor MC...
MCM7
83Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti103I → L in CAA52803 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_029243114R → Q. Corresponds to variant dbSNP:rs2307348Ensembl.1
Natural variantiVAR_013297144N → S1 PublicationCorresponds to variant dbSNP:rs2070215Ensembl.1
Natural variantiVAR_014817473G → S. Corresponds to variant dbSNP:rs2307347Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0443101 – 176Missing in isoform 3. 1 PublicationAdd BLAST176
Alternative sequenceiVSP_003205329 – 658Missing in isoform 2. 1 PublicationAdd BLAST330

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D55716 mRNA Translation: BAA09534.1
AK055379 mRNA Translation: BAG51508.1
AC073842 Genomic DNA No translation available.
CH236956 Genomic DNA Translation: EAL23855.1
CH236956 Genomic DNA Translation: EAL23856.1
CH471091 Genomic DNA Translation: EAW76598.1
CH471091 Genomic DNA Translation: EAW76599.1
BC009398 mRNA Translation: AAH09398.1
BC013375 mRNA Translation: AAH13375.1
X74796 mRNA Translation: CAA52803.1
D28480 mRNA Translation: BAA05839.1
CCDSiCCDS5683.1 [P33993-1]
CCDS5684.1 [P33993-3]
PIRiS70583
RefSeqiNP_001265524.1, NM_001278595.1 [P33993-3]
NP_005907.3, NM_005916.4 [P33993-1]
NP_877577.1, NM_182776.2 [P33993-3]
UniGeneiHs.438720

Genome annotation databases

EnsembliENST00000303887; ENSP00000307288; ENSG00000166508 [P33993-1]
ENST00000343023; ENSP00000344006; ENSG00000166508 [P33993-2]
ENST00000354230; ENSP00000346171; ENSG00000166508 [P33993-3]
ENST00000621318; ENSP00000483795; ENSG00000166508 [P33993-3]
GeneIDi4176
KEGGihsa:4176
UCSCiuc003usv.3 human [P33993-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D55716 mRNA Translation: BAA09534.1
AK055379 mRNA Translation: BAG51508.1
AC073842 Genomic DNA No translation available.
CH236956 Genomic DNA Translation: EAL23855.1
CH236956 Genomic DNA Translation: EAL23856.1
CH471091 Genomic DNA Translation: EAW76598.1
CH471091 Genomic DNA Translation: EAW76599.1
BC009398 mRNA Translation: AAH09398.1
BC013375 mRNA Translation: AAH13375.1
X74796 mRNA Translation: CAA52803.1
D28480 mRNA Translation: BAA05839.1
CCDSiCCDS5683.1 [P33993-1]
CCDS5684.1 [P33993-3]
PIRiS70583
RefSeqiNP_001265524.1, NM_001278595.1 [P33993-3]
NP_005907.3, NM_005916.4 [P33993-1]
NP_877577.1, NM_182776.2 [P33993-3]
UniGeneiHs.438720

3D structure databases

ProteinModelPortaliP33993
SMRiP33993
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110344, 175 interactors
ComplexPortaliCPX-2940 MCM complex
CORUMiP33993
DIPiDIP-27580N
IntActiP33993, 184 interactors
MINTiP33993
STRINGi9606.ENSP00000307288

PTM databases

iPTMnetiP33993
PhosphoSitePlusiP33993
SwissPalmiP33993

Polymorphism and mutation databases

DMDMi20981696

Proteomic databases

EPDiP33993
MaxQBiP33993
PaxDbiP33993
PeptideAtlasiP33993
PRIDEiP33993
ProteomicsDBi54936
54937 [P33993-2]

Protocols and materials databases

DNASUi4176
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000303887; ENSP00000307288; ENSG00000166508 [P33993-1]
ENST00000343023; ENSP00000344006; ENSG00000166508 [P33993-2]
ENST00000354230; ENSP00000346171; ENSG00000166508 [P33993-3]
ENST00000621318; ENSP00000483795; ENSG00000166508 [P33993-3]
GeneIDi4176
KEGGihsa:4176
UCSCiuc003usv.3 human [P33993-1]

Organism-specific databases

CTDi4176
DisGeNETi4176
EuPathDBiHostDB:ENSG00000166508.17
GeneCardsiMCM7
HGNCiHGNC:6950 MCM7
HPAiCAB002163
CAB016312
HPA003898
MIMi600592 gene
neXtProtiNX_P33993
OpenTargetsiENSG00000166508
PharmGKBiPA30697
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0482 Eukaryota
COG1241 LUCA
GeneTreeiENSGT00930000151047
HOGENOMiHOG000224125
HOVERGENiHBG000741
InParanoidiP33993
KOiK02210
OMAiVKIQEMA
OrthoDBiEOG091G02LB
PhylomeDBiP33993
TreeFamiTF300400

Enzyme and pathway databases

ReactomeiR-HSA-176187 Activation of ATR in response to replication stress
R-HSA-176974 Unwinding of DNA
R-HSA-68867 Assembly of the pre-replicative complex
R-HSA-68949 Orc1 removal from chromatin
R-HSA-68962 Activation of the pre-replicative complex
R-HSA-69052 Switching of origins to a post-replicative state
SIGNORiP33993

Miscellaneous databases

ChiTaRSiMCM7 human
GeneWikiiMCM7
GenomeRNAii4176
PROiPR:P33993
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000166508 Expressed in 196 organ(s), highest expression level in embryo
CleanExiHS_MCM2
HS_MCM7
ExpressionAtlasiP33993 baseline and differential
GenevisibleiP33993 HS

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR031327 MCM
IPR008050 MCM7
IPR018525 MCM_CS
IPR001208 MCM_dom
IPR027925 MCM_N
IPR033762 MCM_OB
IPR012340 NA-bd_OB-fold
IPR027417 P-loop_NTPase
PANTHERiPTHR11630 PTHR11630, 1 hit
PTHR11630:SF26 PTHR11630:SF26, 1 hit
PfamiView protein in Pfam
PF00493 MCM, 1 hit
PF14551 MCM_N, 1 hit
PF17207 MCM_OB, 1 hit
PRINTSiPR01657 MCMFAMILY
PR01663 MCMPROTEIN7
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SM00350 MCM, 1 hit
SUPFAMiSSF50249 SSF50249, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00847 MCM_1, 1 hit
PS50051 MCM_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMCM7_HUMAN
AccessioniPrimary (citable) accession number: P33993
Secondary accession number(s): A4D2A1
, A4D2A2, E9PGN9, Q15076, Q96D34, Q96GL1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: May 15, 2002
Last modified: November 7, 2018
This is version 201 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
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