UniProtKB - P33941 (YOJI_ECOLI)
Protein
ABC transporter ATP-binding/permease protein YojI
Gene
yojI
Organism
Escherichia coli (strain K12)
Status
Functioni
Mediates resistance to the antibacterial peptide microcin J25, when expressed from a multicopy vector. Functions as an efflux pump for microcin J25, with the help of the outer membrane channel TolC.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 356 – 363 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATPase activity Source: InterPro
- ATPase-coupled transmembrane transporter activity Source: GO_Central
- ATP binding Source: EcoCyc
- peptide transmembrane transporter activity Source: EcoCyc
GO - Biological processi
- microcin transport Source: EcoliWiki
- response to antibiotic Source: EcoCyc
Keywordsi
Biological process | Antibiotic resistance, Transport |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:YOJI-MONOMER |
Protein family/group databases
TCDBi | 3.A.1.113.3, the atp-binding cassette (abc) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: ABC transporter ATP-binding/permease protein YojICurated |
Gene namesi | Name:yojI Synonyms:yojJ Ordered Locus Names:b2211, JW2199 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane Curated; Multi-pass membrane protein Sequence analysis
Plasma Membrane
- ATP-binding cassette (ABC) transporter complex Source: GO_Central
Other locations
- integral component of membrane Source: EcoCyc
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 16 – 36 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 47 – 67 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 125 – 145 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 149 – 169 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 240 – 260 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 270 – 290 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Disruption increases sensitivity of cells to microcin J25.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000093196 | 1 – 547 | ABC transporter ATP-binding/permease protein YojIAdd BLAST | 547 |
Proteomic databases
jPOSTi | P33941 |
PaxDbi | P33941 |
PRIDEi | P33941 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4261919, 159 interactors |
IntActi | P33941, 5 interactors |
STRINGi | 511145.b2211 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 15 – 291 | ABC transmembrane type-1PROSITE-ProRule annotationAdd BLAST | 277 | |
Domaini | 323 – 547 | ABC transporterPROSITE-ProRule annotationAdd BLAST | 225 |
Sequence similaritiesi
Belongs to the ABC transporter superfamily.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG4615, Bacteria |
HOGENOMi | CLU_023671_1_0_6 |
InParanoidi | P33941 |
PhylomeDBi | P33941 |
Family and domain databases
Gene3Di | 1.20.1560.10, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR011527, ABC1_TM_dom IPR036640, ABC1_TM_sf IPR003439, ABC_transporter-like IPR017871, ABC_transporter_CS IPR005898, Cyc_pep_transpt IPR027417, P-loop_NTPase |
PANTHERi | PTHR43553:SF11, PTHR43553:SF11, 1 hit |
Pfami | View protein in Pfam PF00005, ABC_tran, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF90123, SSF90123, 1 hit |
TIGRFAMsi | TIGR01194, cyc_pep_trnsptr, 1 hit |
PROSITEi | View protein in PROSITE PS50929, ABC_TM1F, 1 hit PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P33941-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MELLVLVWRQ YRWPFISVMA LSLASAALGI GLIAFINQRL IETADTSLLV
60 70 80 90 100
LPEFLGLLLL LMAVTLGSQL ALTTLGHHFV YRLRSEFIKR ILDTHVERIE
110 120 130 140 150
QLGSASLLAG LTSDVRNITI AFVRLPELVQ GIILTIGSAA YLWMLSGKML
160 170 180 190 200
LVTAIWMAIT IWGGFVLVAR VYKHMATLRE TEDKLYTDFQ TVLEGRKELT
210 220 230 240 250
LNRERAEYVF NNLYIPDAQE YRHHIIRADT FHLSAVNWSN IMMLGAIGLV
260 270 280 290 300
FWMANSLGWA DTNVAATYSL TLLFLRTPLL SAVGALPTLL TAQVAFNKLN
310 320 330 340 350
KFALAPFKAE FPRPQAFPNW QTLELRNVTF AYQDNAFSVG PINLTIKRGE
360 370 380 390 400
LLFLIGGNGS GKSTLAMLLT GLYQPQSGEI LLDGKPVSGE QPEDYRKLFS
410 420 430 440 450
AVFTDVWLFD QLLGPEGKPA NPQLVEKWLA QLKMAHKLEL SNGRIVNLKL
460 470 480 490 500
SKGQKKRVAL LLALAEERDI ILLDEWAADQ DPHFRREFYQ VLLPLMQEMG
510 520 530 540
KTIFAISHDD HYFIHADRLL EMRNGQLSEL TGEERDAASR DAVARTA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00008 Genomic DNA Translation: AAA16403.1 U00096 Genomic DNA Translation: AAC75271.1 AP009048 Genomic DNA Translation: BAA15994.1 |
PIRi | A64991 |
RefSeqi | NP_416715.1, NC_000913.3 WP_000422182.1, NZ_SSZK01000030.1 |
Genome annotation databases
EnsemblBacteriai | AAC75271; AAC75271; b2211 BAA15994; BAA15994; BAA15994 |
GeneIDi | 946705 |
KEGGi | ecj:JW2199 eco:b2211 |
PATRICi | fig|1411691.4.peg.24 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00008 Genomic DNA Translation: AAA16403.1 U00096 Genomic DNA Translation: AAC75271.1 AP009048 Genomic DNA Translation: BAA15994.1 |
PIRi | A64991 |
RefSeqi | NP_416715.1, NC_000913.3 WP_000422182.1, NZ_SSZK01000030.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4261919, 159 interactors |
IntActi | P33941, 5 interactors |
STRINGi | 511145.b2211 |
Protein family/group databases
TCDBi | 3.A.1.113.3, the atp-binding cassette (abc) superfamily |
Proteomic databases
jPOSTi | P33941 |
PaxDbi | P33941 |
PRIDEi | P33941 |
Genome annotation databases
EnsemblBacteriai | AAC75271; AAC75271; b2211 BAA15994; BAA15994; BAA15994 |
GeneIDi | 946705 |
KEGGi | ecj:JW2199 eco:b2211 |
PATRICi | fig|1411691.4.peg.24 |
Organism-specific databases
EchoBASEi | EB1997 |
Phylogenomic databases
eggNOGi | COG4615, Bacteria |
HOGENOMi | CLU_023671_1_0_6 |
InParanoidi | P33941 |
PhylomeDBi | P33941 |
Enzyme and pathway databases
BioCyci | EcoCyc:YOJI-MONOMER |
Miscellaneous databases
PROi | PR:P33941 |
Family and domain databases
Gene3Di | 1.20.1560.10, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR011527, ABC1_TM_dom IPR036640, ABC1_TM_sf IPR003439, ABC_transporter-like IPR017871, ABC_transporter_CS IPR005898, Cyc_pep_transpt IPR027417, P-loop_NTPase |
PANTHERi | PTHR43553:SF11, PTHR43553:SF11, 1 hit |
Pfami | View protein in Pfam PF00005, ABC_tran, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF90123, SSF90123, 1 hit |
TIGRFAMsi | TIGR01194, cyc_pep_trnsptr, 1 hit |
PROSITEi | View protein in PROSITE PS50929, ABC_TM1F, 1 hit PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | YOJI_ECOLI | |
Accessioni | P33941Primary (citable) accession number: P33941 Secondary accession number(s): P33942 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | June 1, 1994 | |
Last modified: | December 2, 2020 | |
This is version 166 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families