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UniProtKB - P33908 (MA1A1_HUMAN)
Protein
Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA
Gene
MAN1A1
Organism
Homo sapiens (Human)
Status
Functioni
Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2.
Catalytic activityi
- 4 H2O + N4-(α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 9A1,2,3B1,2,3) = 4 β-D-mannose + N4-(α-D-Man-(1→3)-[α-D-Man-(1→3)-[α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-β-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2)By similarityEC:3.2.1.113By similarity
- 3 H2O + N4-(α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 8A1,2,3B1,3) = 3 β-D-mannose + N4-(α-D-Man-(1→3)-[α-D-Man-(1→3)-[α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-β-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2)By similarityEC:3.2.1.113By similarity
Cofactori
Ca2+By similarity
Activity regulationi
Inhibited by both 1-deoxymannojirimycin and kifunensine.
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarityView all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 522 | Proton donorBy similarity | 1 |
GO - Molecular functioni
- calcium ion binding Source: ProtInc
- mannosidase activity Source: ProtInc
- mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: UniProtKB
GO - Biological processi
- carbohydrate metabolic process Source: InterPro
- Golgi apparatus mannose trimming Source: Reactome
- mannose trimming involved in glycoprotein ERAD pathway Source: UniProtKB
- N-glycan processing Source: GO_Central
- protein glycosylation Source: UniProtKB-UniPathway
- protein targeting to ER Source: UniProtKB
- ubiquitin-dependent ERAD pathway Source: UniProtKB
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Ligand | Calcium |
Enzyme and pathway databases
BRENDAi | 3.2.1.113, 2681 |
PathwayCommonsi | P33908 |
Reactomei | R-HSA-6811438, Intra-Golgi traffic R-HSA-964827, Progressive trimming of alpha-1,2-linked mannose residues from Man9/8/7GlcNAc2 to produce Man5GlcNAc2 |
SignaLinki | P33908 |
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | GH47, Glycoside Hydrolase Family 47 |
Names & Taxonomyi
Protein namesi | Recommended name: Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA (EC:3.2.1.113By similarity)Alternative name(s): Man(9)-alpha-mannosidase Short name: Man9-mannosidase Mannosidase alpha class 1A member 1 Processing alpha-1,2-mannosidase IA Short name: Alpha-1,2-mannosidase IA |
Gene namesi | Name:MAN1A1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:6821, MAN1A1 |
MIMi | 604344, gene |
neXtProti | NX_P33908 |
VEuPathDBi | HostDB:ENSG00000111885 |
Subcellular locationi
Golgi apparatus
Cytosol
- cytosol Source: HPA
Endoplasmic reticulum
- endoplasmic reticulum Source: GO_Central
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Golgi apparatus
- Golgi apparatus Source: HPA
- Golgi membrane Source: GO_Central
Other locations
- cytoplasmic vesicle Source: UniProtKB
- endoplasmic reticulum-Golgi intermediate compartment Source: UniProtKB
- integral component of membrane Source: UniProtKB-KW
- membrane Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 41 | CytoplasmicSequence analysisAdd BLAST | 41 | |
Transmembranei | 42 – 62 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 21 | |
Topological domaini | 63 – 653 | LumenalSequence analysisAdd BLAST | 591 |
Keywords - Cellular componenti
Golgi apparatus, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 4121 |
OpenTargetsi | ENSG00000111885 |
PharmGKBi | PA30570 |
Miscellaneous databases
Pharosi | P33908, Tbio |
Chemistry databases
ChEMBLi | CHEMBL5915 |
Genetic variation databases
BioMutai | MAN1A1 |
DMDMi | 62906886 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000210308 | 1 – 653 | Mannosyl-oligosaccharide 1,2-alpha-mannosidase IAAdd BLAST | 653 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 476 ↔ 508 | By similarity | ||
Glycosylationi | 513 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
EPDi | P33908 |
jPOSTi | P33908 |
MassIVEi | P33908 |
MaxQBi | P33908 |
PaxDbi | P33908 |
PeptideAtlasi | P33908 |
PRIDEi | P33908 |
ProteomicsDBi | 54929 [P33908-1] 66803 |
TopDownProteomicsi | P33908-1 [P33908-1] |
PTM databases
GlyGeni | P33908, 1 site |
iPTMneti | P33908 |
PhosphoSitePlusi | P33908 |
Expressioni
Gene expression databases
Bgeei | ENSG00000111885, Expressed in synovial joint and 250 other tissues |
Genevisiblei | P33908, HS |
Organism-specific databases
HPAi | ENSG00000111885, Tissue enhanced (liver) |
Interactioni
Protein-protein interaction databases
BioGRIDi | 110294, 81 interactors |
IntActi | P33908, 14 interactors |
MINTi | P33908 |
STRINGi | 9606.ENSP00000357453 |
Miscellaneous databases
RNActi | P33908, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 81 – 116 | DisorderedSequence analysisAdd BLAST | 36 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 89 – 116 | Basic and acidic residuesSequence analysisAdd BLAST | 28 |
Sequence similaritiesi
Belongs to the glycosyl hydrolase 47 family.Curated
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2204, Eukaryota |
GeneTreei | ENSGT00940000158188 |
HOGENOMi | CLU_003818_3_2_1 |
InParanoidi | P33908 |
OMAi | QKVMNIR |
OrthoDBi | 693882at2759 |
PhylomeDBi | P33908 |
TreeFami | TF313420 |
Family and domain databases
Gene3Di | 1.50.10.10, 1 hit |
InterProi | View protein in InterPro IPR012341, 6hp_glycosidase-like_sf IPR001382, Glyco_hydro_47 IPR036026, Seven-hairpin_glycosidases |
Pfami | View protein in Pfam PF01532, Glyco_hydro_47, 1 hit |
PRINTSi | PR00747, GLYHDRLASE47 |
SUPFAMi | SSF48225, SSF48225, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: P33908-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MPVGGLLPLF SSPAGGVLGG GLGGGGGRKG SGPAALRLTE KFVLLLVFSA
60 70 80 90 100
FITLCFGAIF FLPDSSKLLS GVLFHSSPAL QPAADHKPGP GARAEDAAEG
110 120 130 140 150
RARRREEGAP GDPEAALEDN LARIRENHER ALREAKETLQ KLPEEIQRDI
160 170 180 190 200
LLEKKKVAQD QLRDKAPFRG LPPVDFVPPI GVESREPADA AIREKRAKIK
210 220 230 240 250
EMMKHAWNNY KGYAWGLNEL KPISKGGHSS SLFGNIKGAT IVDALDTLFI
260 270 280 290 300
MEMKHEFEEA KSWVEENLDF NVNAEISVFE VNIRFVGGLL SAYYLSGEEI
310 320 330 340 350
FRKKAVELGV KLLPAFHTPS GIPWALLNMK SGIGRNWPWA SGGSSILAEF
360 370 380 390 400
GTLHLEFMHL SHLSGNPIFA EKVMNIRTVL NKLEKPQGLY PNYLNPSSGQ
410 420 430 440 450
WGQHHVSVGG LGDSFYEYLL KAWLMSDKTD LEAKKMYFDA VQAIETHLIR
460 470 480 490 500
KSSSGLTYIA EWKGGLLEHK MGHLTCFAGG MFALGADAAP EGMAQHYLEL
510 520 530 540 550
GAEIARTCHE SYNRTFMKLG PEAFRFDGGV EAIATRQNEK YYILRPEVME
560 570 580 590 600
TYMYMWRLTH DPKYRKWAWE AVEALENHCR VNGGYSGLRD VYLLHESYDD
610 620 630 640 650
VQQSFFLAET LKYLYLIFSD DDLLPLEHWI FNSEAHLLPI LPKDKKEVEI
REE
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 29 – 47 | KGSGP…VLLLV → MNSNFITFDLKMSLLPSNL in CAA52831 (PubMed:8223597).CuratedAdd BLAST | 19 | |
Sequence conflicti | 464 | G → R in CAA52831 (PubMed:8223597).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_034102 | 651 | R → G. Corresponds to variant dbSNP:rs35544784EnsemblClinVar. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_056372 | 201 | E → EEIEARKGQANCPGSSSSCE VEIQ in isoform 2. 1 Publication | 1 | |
Alternative sequenceiVSP_056373 | 331 – 344 | SGIGR…ASGGS → RWMCNIPRAMEATI in isoform 2. 1 PublicationAdd BLAST | 14 | |
Alternative sequenceiVSP_056374 | 345 – 653 | Missing in isoform 2. 1 PublicationAdd BLAST | 309 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL022722 Genomic DNA No translation available. AL078600 Genomic DNA No translation available. AL138886 Genomic DNA No translation available. CH471051 Genomic DNA Translation: EAW48185.1 BC065827 mRNA Translation: AAH65827.1 X74837 mRNA Translation: CAA52831.1 |
CCDSi | CCDS5122.1 [P33908-1] |
PIRi | S38965 |
RefSeqi | NP_005898.2, NM_005907.3 [P33908-1] |
Genome annotation databases
Ensembli | ENST00000368468; ENSP00000357453; ENSG00000111885 |
GeneIDi | 4121 |
KEGGi | hsa:4121 |
MANE-Selecti | ENST00000368468.4; ENSP00000357453.3; NM_005907.4; NP_005898.2 |
UCSCi | uc003pym.3, human [P33908-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL022722 Genomic DNA No translation available. AL078600 Genomic DNA No translation available. AL138886 Genomic DNA No translation available. CH471051 Genomic DNA Translation: EAW48185.1 BC065827 mRNA Translation: AAH65827.1 X74837 mRNA Translation: CAA52831.1 |
CCDSi | CCDS5122.1 [P33908-1] |
PIRi | S38965 |
RefSeqi | NP_005898.2, NM_005907.3 [P33908-1] |
3D structure databases
SMRi | P33908 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 110294, 81 interactors |
IntActi | P33908, 14 interactors |
MINTi | P33908 |
STRINGi | 9606.ENSP00000357453 |
Chemistry databases
ChEMBLi | CHEMBL5915 |
Protein family/group databases
CAZyi | GH47, Glycoside Hydrolase Family 47 |
PTM databases
GlyGeni | P33908, 1 site |
iPTMneti | P33908 |
PhosphoSitePlusi | P33908 |
Genetic variation databases
BioMutai | MAN1A1 |
DMDMi | 62906886 |
Proteomic databases
EPDi | P33908 |
jPOSTi | P33908 |
MassIVEi | P33908 |
MaxQBi | P33908 |
PaxDbi | P33908 |
PeptideAtlasi | P33908 |
PRIDEi | P33908 |
ProteomicsDBi | 54929 [P33908-1] 66803 |
TopDownProteomicsi | P33908-1 [P33908-1] |
Protocols and materials databases
Antibodypediai | 32605, 81 antibodies from 19 providers |
DNASUi | 4121 |
Genome annotation databases
Ensembli | ENST00000368468; ENSP00000357453; ENSG00000111885 |
GeneIDi | 4121 |
KEGGi | hsa:4121 |
MANE-Selecti | ENST00000368468.4; ENSP00000357453.3; NM_005907.4; NP_005898.2 |
UCSCi | uc003pym.3, human [P33908-1] |
Organism-specific databases
CTDi | 4121 |
DisGeNETi | 4121 |
GeneCardsi | MAN1A1 |
HGNCi | HGNC:6821, MAN1A1 |
HPAi | ENSG00000111885, Tissue enhanced (liver) |
MIMi | 604344, gene |
neXtProti | NX_P33908 |
OpenTargetsi | ENSG00000111885 |
PharmGKBi | PA30570 |
VEuPathDBi | HostDB:ENSG00000111885 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2204, Eukaryota |
GeneTreei | ENSGT00940000158188 |
HOGENOMi | CLU_003818_3_2_1 |
InParanoidi | P33908 |
OMAi | QKVMNIR |
OrthoDBi | 693882at2759 |
PhylomeDBi | P33908 |
TreeFami | TF313420 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
BRENDAi | 3.2.1.113, 2681 |
PathwayCommonsi | P33908 |
Reactomei | R-HSA-6811438, Intra-Golgi traffic R-HSA-964827, Progressive trimming of alpha-1,2-linked mannose residues from Man9/8/7GlcNAc2 to produce Man5GlcNAc2 |
SignaLinki | P33908 |
Miscellaneous databases
BioGRID-ORCSi | 4121, 6 hits in 1045 CRISPR screens |
ChiTaRSi | MAN1A1, human |
GeneWikii | MAN1A1 |
GenomeRNAii | 4121 |
Pharosi | P33908, Tbio |
PROi | PR:P33908 |
RNActi | P33908, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000111885, Expressed in synovial joint and 250 other tissues |
Genevisiblei | P33908, HS |
Family and domain databases
Gene3Di | 1.50.10.10, 1 hit |
InterProi | View protein in InterPro IPR012341, 6hp_glycosidase-like_sf IPR001382, Glyco_hydro_47 IPR036026, Seven-hairpin_glycosidases |
Pfami | View protein in Pfam PF01532, Glyco_hydro_47, 1 hit |
PRINTSi | PR00747, GLYHDRLASE47 |
SUPFAMi | SSF48225, SSF48225, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MA1A1_HUMAN | |
Accessioni | P33908Primary (citable) accession number: P33908 Secondary accession number(s): E7EU32 Q9UJI3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | April 26, 2005 | |
Last modified: | February 23, 2022 | |
This is version 189 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - Human chromosome 6
Human chromosome 6: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families