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Protein

Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA

Gene

MAN1A1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2.

Catalytic activityi

Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.By similarity

Cofactori

Ca2+By similarity

Activity regulationi

Inhibited by both 1-deoxymannojirimycin and kifunensine.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei522Proton donorBy similarity1

GO - Molecular functioni

  • calcium ion binding Source: ProtInc
  • mannosidase activity Source: ProtInc
  • mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
LigandCalcium

Enzyme and pathway databases

BioCyciMetaCyc:HS03480-MONOMER
ReactomeiR-HSA-6811438 Intra-Golgi traffic
R-HSA-964827 Progressive trimming of alpha-1,2-linked mannose residues from Man9/8/7GlcNAc2 to produce Man5GlcNAc2
UniPathwayi
UPA00378

Protein family/group databases

CAZyiGH47 Glycoside Hydrolase Family 47

Names & Taxonomyi

Protein namesi
Recommended name:
Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA (EC:3.2.1.113By similarity)
Alternative name(s):
Man(9)-alpha-mannosidase
Short name:
Man9-mannosidase
Mannosidase alpha class 1A member 1
Processing alpha-1,2-mannosidase IA
Short name:
Alpha-1,2-mannosidase IA
Gene namesi
Name:MAN1A1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000111885.6
HGNCiHGNC:6821 MAN1A1
MIMi604344 gene
neXtProtiNX_P33908

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 41CytoplasmicSequence analysisAdd BLAST41
Transmembranei42 – 62Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini63 – 653LumenalSequence analysisAdd BLAST591

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi4121
OpenTargetsiENSG00000111885
PharmGKBiPA30570

Chemistry databases

ChEMBLiCHEMBL5915

Polymorphism and mutation databases

BioMutaiMAN1A1
DMDMi62906886

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002103081 – 653Mannosyl-oligosaccharide 1,2-alpha-mannosidase IAAdd BLAST653

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi476 ↔ 508By similarity
Glycosylationi513N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiP33908
MaxQBiP33908
PaxDbiP33908
PeptideAtlasiP33908
PRIDEiP33908
ProteomicsDBi54929
TopDownProteomicsiP33908-1 [P33908-1]

PTM databases

iPTMnetiP33908
PhosphoSitePlusiP33908

Expressioni

Gene expression databases

BgeeiENSG00000111885 Expressed in 238 organ(s), highest expression level in liver
CleanExiHS_MAN1A1
GenevisibleiP33908 HS

Organism-specific databases

HPAiCAB037058
HPA053198
HPA063561

Interactioni

Protein-protein interaction databases

BioGridi110294, 23 interactors
IntActiP33908, 1 interactor
STRINGi9606.ENSP00000357453

Structurei

3D structure databases

ProteinModelPortaliP33908
SMRiP33908
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 47 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2431 Eukaryota
ENOG410XP04 LUCA
GeneTreeiENSGT00390000016529
HOGENOMiHOG000181988
HOVERGENiHBG052389
InParanoidiP33908
KOiK01230
OMAiDHKPGPG
OrthoDBiEOG091G04C8
PhylomeDBiP33908
TreeFamiTF313420

Family and domain databases

Gene3Di1.50.10.10, 1 hit
InterProiView protein in InterPro
IPR012341 6hp_glycosidase-like_sf
IPR001382 Glyco_hydro_47
IPR036026 Seven-hairpin_glycosidases
PfamiView protein in Pfam
PF01532 Glyco_hydro_47, 1 hit
PRINTSiPR00747 GLYHDRLASE47
SUPFAMiSSF48225 SSF48225, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: P33908-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MPVGGLLPLF SSPAGGVLGG GLGGGGGRKG SGPAALRLTE KFVLLLVFSA
60 70 80 90 100
FITLCFGAIF FLPDSSKLLS GVLFHSSPAL QPAADHKPGP GARAEDAAEG
110 120 130 140 150
RARRREEGAP GDPEAALEDN LARIRENHER ALREAKETLQ KLPEEIQRDI
160 170 180 190 200
LLEKKKVAQD QLRDKAPFRG LPPVDFVPPI GVESREPADA AIREKRAKIK
210 220 230 240 250
EMMKHAWNNY KGYAWGLNEL KPISKGGHSS SLFGNIKGAT IVDALDTLFI
260 270 280 290 300
MEMKHEFEEA KSWVEENLDF NVNAEISVFE VNIRFVGGLL SAYYLSGEEI
310 320 330 340 350
FRKKAVELGV KLLPAFHTPS GIPWALLNMK SGIGRNWPWA SGGSSILAEF
360 370 380 390 400
GTLHLEFMHL SHLSGNPIFA EKVMNIRTVL NKLEKPQGLY PNYLNPSSGQ
410 420 430 440 450
WGQHHVSVGG LGDSFYEYLL KAWLMSDKTD LEAKKMYFDA VQAIETHLIR
460 470 480 490 500
KSSSGLTYIA EWKGGLLEHK MGHLTCFAGG MFALGADAAP EGMAQHYLEL
510 520 530 540 550
GAEIARTCHE SYNRTFMKLG PEAFRFDGGV EAIATRQNEK YYILRPEVME
560 570 580 590 600
TYMYMWRLTH DPKYRKWAWE AVEALENHCR VNGGYSGLRD VYLLHESYDD
610 620 630 640 650
VQQSFFLAET LKYLYLIFSD DDLLPLEHWI FNSEAHLLPI LPKDKKEVEI

REE
Length:653
Mass (Da):72,969
Last modified:April 26, 2005 - v3
Checksum:i9B164926636D8554
GO
Isoform 2 (identifier: P33908-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     201-201: E → EEIEARKGQANCPGSSSSCEVEIQ
     331-344: SGIGRNWPWASGGS → RWMCNIPRAMEATI
     345-653: Missing.

Note: No experimental confirmation available.
Show »
Length:367
Mass (Da):40,037
Checksum:i3A5E1D30321CAFF8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti29 – 47KGSGP…VLLLV → MNSNFITFDLKMSLLPSNL in CAA52831 (PubMed:8223597).CuratedAdd BLAST19
Sequence conflicti464G → R in CAA52831 (PubMed:8223597).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_034102651R → G. Corresponds to variant dbSNP:rs35544784Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_056372201E → EEIEARKGQANCPGSSSSCE VEIQ in isoform 2. 1 Publication1
Alternative sequenceiVSP_056373331 – 344SGIGR…ASGGS → RWMCNIPRAMEATI in isoform 2. 1 PublicationAdd BLAST14
Alternative sequenceiVSP_056374345 – 653Missing in isoform 2. 1 PublicationAdd BLAST309

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL022722 Genomic DNA No translation available.
AL078600 Genomic DNA No translation available.
AL138886 Genomic DNA No translation available.
CH471051 Genomic DNA Translation: EAW48185.1
BC065827 mRNA Translation: AAH65827.1
X74837 mRNA Translation: CAA52831.1
CCDSiCCDS5122.1 [P33908-1]
PIRiS38965
RefSeqiNP_005898.2, NM_005907.3 [P33908-1]
UniGeneiHs.102788
Hs.742378

Genome annotation databases

EnsembliENST00000368468; ENSP00000357453; ENSG00000111885 [P33908-1]
GeneIDi4121
KEGGihsa:4121
UCSCiuc003pym.3 human [P33908-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL022722 Genomic DNA No translation available.
AL078600 Genomic DNA No translation available.
AL138886 Genomic DNA No translation available.
CH471051 Genomic DNA Translation: EAW48185.1
BC065827 mRNA Translation: AAH65827.1
X74837 mRNA Translation: CAA52831.1
CCDSiCCDS5122.1 [P33908-1]
PIRiS38965
RefSeqiNP_005898.2, NM_005907.3 [P33908-1]
UniGeneiHs.102788
Hs.742378

3D structure databases

ProteinModelPortaliP33908
SMRiP33908
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110294, 23 interactors
IntActiP33908, 1 interactor
STRINGi9606.ENSP00000357453

Chemistry databases

ChEMBLiCHEMBL5915

Protein family/group databases

CAZyiGH47 Glycoside Hydrolase Family 47

PTM databases

iPTMnetiP33908
PhosphoSitePlusiP33908

Polymorphism and mutation databases

BioMutaiMAN1A1
DMDMi62906886

Proteomic databases

EPDiP33908
MaxQBiP33908
PaxDbiP33908
PeptideAtlasiP33908
PRIDEiP33908
ProteomicsDBi54929
TopDownProteomicsiP33908-1 [P33908-1]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000368468; ENSP00000357453; ENSG00000111885 [P33908-1]
GeneIDi4121
KEGGihsa:4121
UCSCiuc003pym.3 human [P33908-1]

Organism-specific databases

CTDi4121
DisGeNETi4121
EuPathDBiHostDB:ENSG00000111885.6
GeneCardsiMAN1A1
HGNCiHGNC:6821 MAN1A1
HPAiCAB037058
HPA053198
HPA063561
MIMi604344 gene
neXtProtiNX_P33908
OpenTargetsiENSG00000111885
PharmGKBiPA30570
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2431 Eukaryota
ENOG410XP04 LUCA
GeneTreeiENSGT00390000016529
HOGENOMiHOG000181988
HOVERGENiHBG052389
InParanoidiP33908
KOiK01230
OMAiDHKPGPG
OrthoDBiEOG091G04C8
PhylomeDBiP33908
TreeFamiTF313420

Enzyme and pathway databases

UniPathwayi
UPA00378

BioCyciMetaCyc:HS03480-MONOMER
ReactomeiR-HSA-6811438 Intra-Golgi traffic
R-HSA-964827 Progressive trimming of alpha-1,2-linked mannose residues from Man9/8/7GlcNAc2 to produce Man5GlcNAc2

Miscellaneous databases

ChiTaRSiMAN1A1 human
GeneWikiiMAN1A1
GenomeRNAii4121
PROiPR:P33908
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000111885 Expressed in 238 organ(s), highest expression level in liver
CleanExiHS_MAN1A1
GenevisibleiP33908 HS

Family and domain databases

Gene3Di1.50.10.10, 1 hit
InterProiView protein in InterPro
IPR012341 6hp_glycosidase-like_sf
IPR001382 Glyco_hydro_47
IPR036026 Seven-hairpin_glycosidases
PfamiView protein in Pfam
PF01532 Glyco_hydro_47, 1 hit
PRINTSiPR00747 GLYHDRLASE47
SUPFAMiSSF48225 SSF48225, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMA1A1_HUMAN
AccessioniPrimary (citable) accession number: P33908
Secondary accession number(s): E7EU32
, Q6P052, Q9NU44, Q9UJI3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: April 26, 2005
Last modified: November 7, 2018
This is version 169 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  7. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
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Main funding by: National Institutes of Health

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