UniProtKB - P33883 (LASI_PSEAE)
Protein
Acyl-homoserine-lactone synthase
Gene
lasI
Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Functioni
Required for the synthesis of PAI consisting of 3-oxo-N-(tetrahydro-2-oxo-3-furanyl)-dodecanamide also known as N-(3-oxododecanoyl)homoserine lactone, an autoinducer molecule which binds to LasR and thus acts in elastase biosynthesis regulation.
Catalytic activityi
- a fatty acyl-[ACP] + S-adenosyl-L-methionine = an N-acyl-L-homoserine lactone + H+ + holo-[ACP] + S-methyl-5'-thioadenosineEC:2.3.1.184
GO - Molecular functioni
- N-acyl homoserine lactone synthase activity Source: UniProtKB-EC
GO - Biological processi
- quorum sensing Source: UniProtKB-KW
- signal transduction Source: PseudoCAP
Keywordsi
Molecular function | Transferase |
Biological process | Autoinducer synthesis, Quorum sensing |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BioCyci | MetaCyc:MONOMER-14567 PAER208964:G1FZ6-1458-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Acyl-homoserine-lactone synthase (EC:2.3.1.184)Alternative name(s): Autoinducer synthesis protein LasI |
Gene namesi | Name:lasI Ordered Locus Names:PA1432 |
Organismi | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) |
Taxonomic identifieri | 208964 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › |
Proteomesi |
|
Organism-specific databases
PseudoCAPi | PA1432 |
Pathology & Biotechi
Miscellaneous databases
PHI-basei | PHI:6997 |
Chemistry databases
ChEMBLi | CHEMBL1795157 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000210887 | 1 – 201 | Acyl-homoserine-lactone synthaseAdd BLAST | 201 |
Proteomic databases
PaxDbi | P33883 |
PRIDEi | P33883 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P33883 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P33883 |
Family & Domainsi
Sequence similaritiesi
Belongs to the autoinducer synthase family.PROSITE-ProRule annotation
Phylogenomic databases
HOGENOMi | CLU_085711_4_0_6 |
OMAi | YMLKDTF |
PhylomeDBi | P33883 |
Family and domain databases
InterProi | View protein in InterPro IPR016181, Acyl_CoA_acyltransferase IPR018311, Autoind_synth_CS IPR001690, Autoind_synthase |
PANTHERi | PTHR39322, PTHR39322, 1 hit |
Pfami | View protein in Pfam PF00765, Autoind_synth, 1 hit |
PRINTSi | PR01549, AUTOINDCRSYN |
SUPFAMi | SSF55729, SSF55729, 1 hit |
PROSITEi | View protein in PROSITE PS00949, AUTOINDUCER_SYNTH_1, 1 hit PS51187, AUTOINDUCER_SYNTH_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P33883-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIVQIGRREE FDKKLLGEMH KLRAQVFKER KGWDVSVIDE MEIDGYDALS
60 70 80 90 100
PYYMLIQEDT PEAQVFGCWR ILDTTGPYML KNTFPELLHG KEAPCSPHIW
110 120 130 140 150
ELSRFAINSG QKGSLGFSDC TLEAMRALAR YSLQNDIQTL VTVTTVGVEK
160 170 180 190 200
MMIRAGLDVS RFGPHLKIGI ERAVALRIEL NAKTQIALYG GVLVEQRLAV
S
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L04681 Genomic DNA Translation: AAA25875.1 AE004091 Genomic DNA Translation: AAG04821.1 |
PIRi | G83467 |
RefSeqi | NP_250123.1, NC_002516.2 WP_003083017.1, NZ_QZGE01000005.1 |
Genome annotation databases
EnsemblBacteriai | AAG04821; AAG04821; PA1432 |
GeneIDi | 881777 |
KEGGi | pae:PA1432 |
PATRICi | fig|208964.12.peg.1481 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L04681 Genomic DNA Translation: AAA25875.1 AE004091 Genomic DNA Translation: AAG04821.1 |
PIRi | G83467 |
RefSeqi | NP_250123.1, NC_002516.2 WP_003083017.1, NZ_QZGE01000005.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1RO5 | X-ray | 2.30 | A | 1-201 | [»] | |
SMRi | P33883 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Chemistry databases
ChEMBLi | CHEMBL1795157 |
Proteomic databases
PaxDbi | P33883 |
PRIDEi | P33883 |
Protocols and materials databases
DNASUi | 881777 |
Genome annotation databases
EnsemblBacteriai | AAG04821; AAG04821; PA1432 |
GeneIDi | 881777 |
KEGGi | pae:PA1432 |
PATRICi | fig|208964.12.peg.1481 |
Organism-specific databases
PseudoCAPi | PA1432 |
Phylogenomic databases
HOGENOMi | CLU_085711_4_0_6 |
OMAi | YMLKDTF |
PhylomeDBi | P33883 |
Enzyme and pathway databases
BioCyci | MetaCyc:MONOMER-14567 PAER208964:G1FZ6-1458-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P33883 |
PHI-basei | PHI:6997 |
Family and domain databases
InterProi | View protein in InterPro IPR016181, Acyl_CoA_acyltransferase IPR018311, Autoind_synth_CS IPR001690, Autoind_synthase |
PANTHERi | PTHR39322, PTHR39322, 1 hit |
Pfami | View protein in Pfam PF00765, Autoind_synth, 1 hit |
PRINTSi | PR01549, AUTOINDCRSYN |
SUPFAMi | SSF55729, SSF55729, 1 hit |
PROSITEi | View protein in PROSITE PS00949, AUTOINDUCER_SYNTH_1, 1 hit PS51187, AUTOINDUCER_SYNTH_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LASI_PSEAE | |
Accessioni | P33883Primary (citable) accession number: P33883 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | February 1, 1994 | |
Last modified: | April 7, 2021 | |
This is version 117 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families