UniProtKB - P33775 (PMT1_YEAST)
Dolichyl-phosphate-mannose--protein mannosyltransferase 1
PMT1
Functioni
Protein O-mannosyltransferase involved in O-glycosylation which is essential for cell wall rigidity. Forms a heterodimeric complex with PMT2 and more rarely with PMT3 to transfer mannose from Dol-P-mannose to Ser or Thr residues on proteins. The PMT1-PMT2 complex participates in oxidative protein folding, ER-associated protein degradation (ERAD), as well as ER export. Required for incorporation of proteins in the cell wall.
5 PublicationsMiscellaneous
Catalytic activityi
- dolichyl β-D-mannosyl phosphate + L-seryl-[protein] = 3-O-(α-D-mannosyl)-L-seryl-[protein] + dolichyl phosphate + H+2 PublicationsEC:2.4.1.1092 Publications
- dolichyl β-D-mannosyl phosphate + L-threonyl-[protein] = 3-O-(α-D-mannosyl)-L-threonyl-[protein] + dolichyl phosphate + H+2 PublicationsEC:2.4.1.1092 Publications
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.CuratedView all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
GO - Molecular functioni
GO - Biological processi
- ER-associated misfolded protein catabolic process Source: SGD
- fungal-type cell wall biogenesis Source: ComplexPortal
- protein exit from endoplasmic reticulum Source: SGD
- protein O-linked mannosylation Source: SGD
- regulation of endoplasmic reticulum unfolded protein response Source: SGD
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Enzyme and pathway databases
BRENDAi | 2.4.1.109, 984 |
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | GT39, Glycosyltransferase Family 39 |
TCDBi | 9.B.142.5.6, the integral membrane glycosyltransferase family 39 (gt39) family |
Names & Taxonomyi
Protein namesi | Recommended name: Dolichyl-phosphate-mannose--protein mannosyltransferase 1Curated (EC:2.4.1.1092 Publications) |
Gene namesi | Name:PMT11 Publication Ordered Locus Names:YDL095WImported ORF Names:D2390Imported |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000002253, PMT1 |
VEuPathDBi | FungiDB:YDL095W |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication
Endoplasmic reticulum
- dolichyl-phosphate-mannose-protein mannosyltransferase Pmt1p-Pmt2p dimer complex Source: SGD
- dolichyl-phosphate-mannose-protein mannosyltransferase Pmt1p-Pmt3p dimer complex Source: SGD
- endoplasmic reticulum Source: SGD
- endoplasmic reticulum membrane Source: ComplexPortal
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 2 – 50 | Cytoplasmic1 PublicationAdd BLAST | 49 | |
Transmembranei | 51 – 70 | Helical1 PublicationAdd BLAST | 20 | |
Topological domaini | 71 – 135 | Lumenal1 PublicationAdd BLAST | 65 | |
Transmembranei | 136 – 154 | Helical1 PublicationAdd BLAST | 19 | |
Topological domaini | 155 – 179 | Cytoplasmic1 PublicationAdd BLAST | 25 | |
Transmembranei | 180 – 200 | Helical1 PublicationAdd BLAST | 21 | |
Topological domaini | 201 – 234 | Lumenal1 PublicationAdd BLAST | 34 | |
Transmembranei | 235 – 259 | Helical1 PublicationAdd BLAST | 25 | |
Topological domaini | 260 – 273 | Cytoplasmic1 PublicationAdd BLAST | 14 | |
Transmembranei | 274 – 291 | Helical1 PublicationAdd BLAST | 18 | |
Topological domaini | 292 – 584 | Lumenal1 PublicationAdd BLAST | 293 | |
Transmembranei | 585 – 605 | Helical1 PublicationAdd BLAST | 21 | |
Topological domaini | 606 – 685 | Cytoplasmic1 PublicationAdd BLAST | 80 | |
Transmembranei | 686 – 710 | Helical1 PublicationAdd BLAST | 25 | |
Topological domaini | 711 – 817 | Lumenal1 PublicationAdd BLAST | 107 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 64 | R → A: Reduces mannosyltransferase activity. 1 Publication | 1 | |
Mutagenesisi | 77 – 78 | DE → AA: Impairs mannosyltransferase activity. 1 Publication | 2 | |
Mutagenesisi | 78 | E → A: Decreases substrate-binding and reduces mannosyltransferase activity. 2 Publications | 1 | |
Mutagenesisi | 88 | Y → A: Moderately decreases complex formation with PMT2. 1 Publication | 1 | |
Mutagenesisi | 100 | P → A: Moderately decreases complex formation with PMT2. 1 Publication | 1 | |
Mutagenesisi | 138 | R → A: Impairs complex formation with PMT2. 1 Publication | 1 | |
Mutagenesisi | 408 | L → A: Reduces mannosyltransferase activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000121491 | 2 – 817 | Dolichyl-phosphate-mannose--protein mannosyltransferase 1Add BLAST | 816 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources | 1 | |
Glycosylationi | 390 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 513 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 743 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Acetylation, GlycoproteinProteomic databases
MaxQBi | P33775 |
PaxDbi | P33775 |
PRIDEi | P33775 |
PTM databases
iPTMneti | P33775 |
Interactioni
Subunit structurei
PMT1 and PMT2 form a functional heterodimer. The complex interacts with endoplasmic reticulum proteins EMP24, ERV25, ERP1, ERP2, CDC48, HRD1, USA1, YOS9, ERO1, PDI1, UBR1, Cue4, DFM1 and TED1.
Forms also a minor complex with PMT3.
3 PublicationsBinary interactionsi
Protein-protein interaction databases
BioGRIDi | 31966, 216 interactors |
ComplexPortali | CPX-3036, PMT1-PMT2 dolichyl-phosphate-mannose-protein mannosyltransferase complex CPX-3037, PMT1-PMT3 dolichyl-phosphate-mannose-protein mannosyltransferase complex |
DIPi | DIP-7911N |
IntActi | P33775, 2 interactors |
MINTi | P33775 |
STRINGi | 4932.YDL095W |
Miscellaneous databases
RNActi | P33775, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P33775 |
SMRi | P33775 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 324 – 378 | MIR 1PROSITE-ProRule annotationAdd BLAST | 55 | |
Domaini | 388 – 448 | MIR 2PROSITE-ProRule annotationAdd BLAST | 61 | |
Domaini | 459 – 514 | MIR 3PROSITE-ProRule annotationAdd BLAST | 56 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3359, Eukaryota |
GeneTreei | ENSGT00940000176667 |
HOGENOMi | CLU_008438_2_0_1 |
InParanoidi | P33775 |
OMAi | TLSVKWV |
Family and domain databases
InterProi | View protein in InterPro IPR027005, GlyclTrfase_39-like IPR003342, Glyco_trans_39/83 IPR036300, MIR_dom_sf IPR016093, MIR_motif IPR027004, PMT1/PTM5 IPR032421, PMT_4TMC |
PANTHERi | PTHR10050, PTHR10050, 1 hit PTHR10050:SF50, PTHR10050:SF50, 1 hit |
Pfami | View protein in Pfam PF02815, MIR, 1 hit PF02366, PMT, 1 hit PF16192, PMT_4TMC, 1 hit |
SMARTi | View protein in SMART SM00472, MIR, 3 hits |
SUPFAMi | SSF82109, SSF82109, 1 hit |
PROSITEi | View protein in PROSITE PS50919, MIR, 3 hits |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MSEEKTYKRV EQDDPVPELD IKQGPVRPFI VTDPSAELAS LRTMVTLKEK
60 70 80 90 100
LLVACLAVFT AVIRLHGLAW PDSVVFDEVH FGGFASQYIR GTYFMDVHPP
110 120 130 140 150
LAKMLYAGVA SLGGFQGDFD FENIGDSFPS TTPYVLMRFF SASLGALTVI
160 170 180 190 200
LMYMTLRYSG VRMWVALMSA ICFAVENSYV TISRYILLDA PLMFFIAAAV
210 220 230 240 250
YSFKKYEMYP ANSLNAYKSL LATGIALGMA SSSKWVGLFT VTWVGLLCIW
260 270 280 290 300
RLWFMIGDLT KSSKSIFKVA FAKLAFLLGV PFALYLVFFY IHFQSLTLDG
310 320 330 340 350
DGASFFSPEF RSTLKNNKIP QNVVADVGIG SIISLRHLST MGGYLHSHSH
360 370 380 390 400
NYPAGSEQQQ STLYPHMDAN NDWLLELYNA PGESLTTFQN LTDGTKVRLF
410 420 430 440 450
HTVTRCRLHS HDHKPPVSES SDWQKEVSCY GYSGFDGDAN DDWVVEIDKK
460 470 480 490 500
NSAPGVAQER VIALDTKFRL RHAMTGCYLF SHEVKLPAWG FEQQEVTCAS
510 520 530 540 550
SGRHDLTLWY VENNSNPLLP EDTKRISYKP ASFISKFIES HKKMWHINKN
560 570 580 590 600
LVEPHVYESQ PTSWPFLLRG ISYWGENNRN VYLLGNAIVW WAVTAFIGIF
610 620 630 640 650
GLIVITELFS WQLGKPILKD SKVVNFHVQV IHYLLGFAVH YAPSFLMQRQ
660 670 680 690 700
MFLHHYLPAY YFGILALGHA LDIIVSYVFR SKRQMGYAVV ITFLAASVYF
710 720 730 740 750
FKSFSPIIYG TPWTQELCQK SQWLSGWDYN CNTYFSSLEE YKNQTLTKRE
760 770 780 790 800
SQPAATSTVE EITIEGDGPS YEDLMNEDGK KIFKDTEGNE LDPEVVKKML
810
EEEGANILKV EKRAVLE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L19169 Unassigned DNA Translation: AAA02928.1 X95644 Genomic DNA Translation: CAA64917.1 Z74144 Genomic DNA Translation: CAA98663.1 BK006938 Genomic DNA Translation: DAA11764.1 |
PIRi | A47716 |
RefSeqi | NP_010188.1, NM_001180154.1 |
Genome annotation databases
EnsemblFungii | YDL095W_mRNA; YDL095W; YDL095W |
GeneIDi | 851462 |
KEGGi | sce:YDL095W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L19169 Unassigned DNA Translation: AAA02928.1 X95644 Genomic DNA Translation: CAA64917.1 Z74144 Genomic DNA Translation: CAA98663.1 BK006938 Genomic DNA Translation: DAA11764.1 |
PIRi | A47716 |
RefSeqi | NP_010188.1, NM_001180154.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6P25 | electron microscopy | 3.20 | A | 1-817 | [»] | |
6P2R | electron microscopy | 3.20 | A | 1-817 | [»] | |
AlphaFoldDBi | P33775 | |||||
SMRi | P33775 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 31966, 216 interactors |
ComplexPortali | CPX-3036, PMT1-PMT2 dolichyl-phosphate-mannose-protein mannosyltransferase complex CPX-3037, PMT1-PMT3 dolichyl-phosphate-mannose-protein mannosyltransferase complex |
DIPi | DIP-7911N |
IntActi | P33775, 2 interactors |
MINTi | P33775 |
STRINGi | 4932.YDL095W |
Protein family/group databases
CAZyi | GT39, Glycosyltransferase Family 39 |
TCDBi | 9.B.142.5.6, the integral membrane glycosyltransferase family 39 (gt39) family |
PTM databases
iPTMneti | P33775 |
Proteomic databases
MaxQBi | P33775 |
PaxDbi | P33775 |
PRIDEi | P33775 |
Genome annotation databases
EnsemblFungii | YDL095W_mRNA; YDL095W; YDL095W |
GeneIDi | 851462 |
KEGGi | sce:YDL095W |
Organism-specific databases
SGDi | S000002253, PMT1 |
VEuPathDBi | FungiDB:YDL095W |
Phylogenomic databases
eggNOGi | KOG3359, Eukaryota |
GeneTreei | ENSGT00940000176667 |
HOGENOMi | CLU_008438_2_0_1 |
InParanoidi | P33775 |
OMAi | TLSVKWV |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
BRENDAi | 2.4.1.109, 984 |
Miscellaneous databases
PROi | PR:P33775 |
RNActi | P33775, protein |
Family and domain databases
InterProi | View protein in InterPro IPR027005, GlyclTrfase_39-like IPR003342, Glyco_trans_39/83 IPR036300, MIR_dom_sf IPR016093, MIR_motif IPR027004, PMT1/PTM5 IPR032421, PMT_4TMC |
PANTHERi | PTHR10050, PTHR10050, 1 hit PTHR10050:SF50, PTHR10050:SF50, 1 hit |
Pfami | View protein in Pfam PF02815, MIR, 1 hit PF02366, PMT, 1 hit PF16192, PMT_4TMC, 1 hit |
SMARTi | View protein in SMART SM00472, MIR, 3 hits |
SUPFAMi | SSF82109, SSF82109, 1 hit |
PROSITEi | View protein in PROSITE PS50919, MIR, 3 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | PMT1_YEAST | |
Accessioni | P33775Primary (citable) accession number: P33775 Secondary accession number(s): D6VRQ4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | February 1, 1994 | |
Last modified: | May 25, 2022 | |
This is version 192 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families