UniProtKB - P33767 (OSTB_YEAST)
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1
WBP1
Functioni
Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc3Man9GlcNAc2 in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.
2 PublicationsMiscellaneous
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.1 PublicationView all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
GO - Molecular functioni
- glycosyltransferase activity Source: SGD
GO - Biological processi
- protein N-linked glycosylation Source: ComplexPortal
- protein N-linked glycosylation via asparagine Source: GO_Central
Enzyme and pathway databases
BRENDAi | 2.4.99.18, 984 |
Reactomei | R-SCE-6798695, Neutrophil degranulation |
UniPathwayi | UPA00378 |
Protein family/group databases
TCDBi | 9.B.142.3.14, the integral membrane glycosyltransferase family 39 (gt39) family |
Names & Taxonomyi
Protein namesi | Recommended name: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1Short name: Oligosaccharyl transferase subunit WBP1 Alternative name(s): Oligosaccharyl transferase subunit beta |
Gene namesi | Name:WBP1 Ordered Locus Names:YEL002C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000000728, WBP1 |
VEuPathDBi | FungiDB:YEL002C |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 3 Publications; Single-pass type I membrane protein 1 Publication
Endoplasmic reticulum
- endoplasmic reticulum Source: SGD
- endoplasmic reticulum membrane Source: SGD
- oligosaccharyltransferase complex Source: SGD
Nucleus
- nuclear envelope Source: SGD
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 24 – 393 | Lumenal1 PublicationAdd BLAST | 370 | |
Transmembranei | 394 – 414 | Helical1 PublicationAdd BLAST | 21 | |
Topological domaini | 415 – 430 | Cytoplasmic1 PublicationAdd BLAST | 16 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 20 | 2 PublicationsAdd BLAST | 20 | |
ChainiPRO_0000021961 | 21 – 430 | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1Add BLAST | 410 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 60 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 332 | N-linked (GlcNAc...) asparagine1 Publication | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
MaxQBi | P33767 |
PaxDbi | P33767 |
PRIDEi | P33767 |
PTM databases
iPTMneti | P33767 |
Interactioni
Subunit structurei
Component of the oligosaccharyltransferase (OST) complex, which appears to exist in two assemblies comprising OST1, OST2, OST4, OST5, STT3, SWP1, WPB1, and either OST3 or OST6 (PubMed:8175708, PubMed:16297388, PubMed:16096345, PubMed:15831493, PubMed:15886282, PubMed:9405463, PubMed:29301962). OST assembly occurs through the formation of 3 subcomplexes. Subcomplex 1 contains OST1 and OST5, subcomplex 2 contains STT3, OST3, and OST4, and subcomplex 3 contains OST2, WBP1, and SWP1 (PubMed:29301962).
Interacts with SEC61, SBH1 and SSS1 (PubMed:15831493).
7 PublicationsBinary interactionsi
P33767
Protein-protein interaction databases
BioGRIDi | 36729, 474 interactors |
ComplexPortali | CPX-1638, Oligosaccharyl transferase complex variant 1 CPX-1639, Oligosaccharyl transferase complex variant 2 |
DIPi | DIP-676N |
ELMi | P33767 |
IntActi | P33767, 44 interactors |
MINTi | P33767 |
STRINGi | 4932.YEL002C |
Miscellaneous databases
RNActi | P33767, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P33767 |
SMRi | P33767 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domaini
Sequence similaritiesi
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2754, Eukaryota |
GeneTreei | ENSGT00390000017294 |
HOGENOMi | CLU_031804_1_1_1 |
InParanoidi | P33767 |
OMAi | YQFKVDY |
Family and domain databases
InterProi | View protein in InterPro IPR005013, DDOST_48_kDa_subunit |
PANTHERi | PTHR10830, PTHR10830, 1 hit |
Pfami | View protein in Pfam PF03345, DDOST_48kD, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MRTDWNFFFC ILLQAIFVVG TQTSRTLVLY DQSTEPLEEY SVYLKDLEQR
60 70 80 90 100
NYKLEYLDIN STSTTVDLYD KEQRLFDNII VFPTKGGKNL ARQIPVKQLI
110 120 130 140 150
KFFENEGNIL CMSSPGAVPN TIRLFLNELG IYPSPKGHVI RDYFSPSSEE
160 170 180 190 200
LVVSSNHLLN KYVYNARKSE DFVFGESSAA LLENREQIVP ILNAPRTSFT
210 220 230 240 250
ESKGKCNSWT SGSQGFLVVG FQNLNNARLV WIGSSDFLKN KNQDSNQEFA
260 270 280 290 300
KELLKWTFNE KSVIKSVHAV HSHADGTSYD EEPYKIKDKV IYSVGFSEWN
310 320 330 340 350
GEEWLPHIAD DIQFELRQVD PYYRLTLSPS GNDSETQYYT TGEFILPDRH
360 370 380 390 400
GVFTFLTDYR KIGLSFTTDK DVKAIRHLAN DEYPRSWEIS NSWVYISAIC
410 420 430
GVIVAWIFFV VSFVTTSSVG KKLETFKKTN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X61388 Genomic DNA Translation: CAA43660.1 U18530 Genomic DNA Translation: AAB64479.1 BK006939 Genomic DNA Translation: DAA07649.1 |
PIRi | S20187 |
RefSeqi | NP_010914.3, NM_001178817.3 |
Genome annotation databases
EnsemblFungii | YEL002C_mRNA; YEL002C; YEL002C |
GeneIDi | 856716 |
KEGGi | sce:YEL002C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X61388 Genomic DNA Translation: CAA43660.1 U18530 Genomic DNA Translation: AAB64479.1 BK006939 Genomic DNA Translation: DAA07649.1 |
PIRi | S20187 |
RefSeqi | NP_010914.3, NM_001178817.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4J86 | X-ray | 1.48 | C/D | 425-430 | [»] | |
6C26 | electron microscopy | 3.50 | B | 1-430 | [»] | |
6EZN | electron microscopy | 3.30 | G | 1-430 | [»] | |
AlphaFoldDBi | P33767 | |||||
SMRi | P33767 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 36729, 474 interactors |
ComplexPortali | CPX-1638, Oligosaccharyl transferase complex variant 1 CPX-1639, Oligosaccharyl transferase complex variant 2 |
DIPi | DIP-676N |
ELMi | P33767 |
IntActi | P33767, 44 interactors |
MINTi | P33767 |
STRINGi | 4932.YEL002C |
Protein family/group databases
TCDBi | 9.B.142.3.14, the integral membrane glycosyltransferase family 39 (gt39) family |
PTM databases
iPTMneti | P33767 |
Proteomic databases
MaxQBi | P33767 |
PaxDbi | P33767 |
PRIDEi | P33767 |
Protocols and materials databases
ABCDi | P33767, 1 sequenced antibody |
Genome annotation databases
EnsemblFungii | YEL002C_mRNA; YEL002C; YEL002C |
GeneIDi | 856716 |
KEGGi | sce:YEL002C |
Organism-specific databases
SGDi | S000000728, WBP1 |
VEuPathDBi | FungiDB:YEL002C |
Phylogenomic databases
eggNOGi | KOG2754, Eukaryota |
GeneTreei | ENSGT00390000017294 |
HOGENOMi | CLU_031804_1_1_1 |
InParanoidi | P33767 |
OMAi | YQFKVDY |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
BRENDAi | 2.4.99.18, 984 |
Reactomei | R-SCE-6798695, Neutrophil degranulation |
Miscellaneous databases
PROi | PR:P33767 |
RNActi | P33767, protein |
Family and domain databases
InterProi | View protein in InterPro IPR005013, DDOST_48_kDa_subunit |
PANTHERi | PTHR10830, PTHR10830, 1 hit |
Pfami | View protein in Pfam PF03345, DDOST_48kD, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | OSTB_YEAST | |
Accessioni | P33767Primary (citable) accession number: P33767 Secondary accession number(s): D3DLP5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | February 1, 1994 | |
Last modified: | May 25, 2022 | |
This is version 187 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome V
Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families