UniProtKB - P33755 (NPL4_YEAST)
Nuclear protein localization protein 4
NPL4
Functioni
Miscellaneous
GO - Molecular functioni
- ubiquitin binding Source: GO_Central
- ubiquitin protein ligase binding Source: GO_Central
GO - Biological processi
- cytoplasm protein quality control by the ubiquitin-proteasome system Source: SGD
- DNA replication termination Source: SGD
- ER-associated misfolded protein catabolic process Source: SGD
- mitochondria-associated ubiquitin-dependent protein catabolic process Source: UniProtKB
- mitotic spindle disassembly Source: SGD
- mRNA transport Source: UniProtKB-KW
- negative regulation of telomerase activity Source: SGD
- nonfunctional rRNA decay Source: SGD
- positive regulation of protein localization to nucleus Source: SGD
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: SGD
- protein localization to vacuole Source: SGD
- retrograde protein transport, ER to cytosol Source: SGD
- ribosome-associated ubiquitin-dependent protein catabolic process Source: SGD
- ubiquitin-dependent ERAD pathway Source: SGD
- ubiquitin-dependent protein catabolic process Source: GO_Central
Keywordsi
Biological process | mRNA transport, Protein transport, Translocation, Transport |
Protein family/group databases
TCDBi | 3.A.16.1.2, the endoplasmic reticular retrotranslocon (er-rt) family |
Names & Taxonomyi
Protein namesi | Recommended name: Nuclear protein localization protein 4Alternative name(s): HMG-CoA reductase degradation protein 4 |
Gene namesi | Name:NPL4 Synonyms:HRD4 Ordered Locus Names:YBR170C ORF Names:YBR1231 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000000374, NPL4 |
VEuPathDBi | FungiDB:YBR170C |
Subcellular locationi
Nucleus
Endoplasmic reticulum
Other locations
Note: Localizes mainly at the nuclear periphery and the endoplasmic reticulum membrane.
Endoplasmic reticulum
- Doa10p ubiquitin ligase complex Source: SGD
- Hrd1p ubiquitin ligase ERAD-L complex Source: SGD
- VCP-NPL4-UFD1 AAA ATPase complex Source: SGD
Mitochondrion
- Cdc48p-Npl4p-Vms1p AAA ATPase complex Source: SGD
Nucleus
- nuclear membrane Source: UniProtKB-SubCell
- nucleus Source: SGD
Other locations
- cytoplasm Source: SGD
- nuclear outer membrane-endoplasmic reticulum membrane network Source: SGD
- perinuclear region of cytoplasm Source: UniProtKB-SubCell
- replisome Source: SGD
- RQC complex Source: SGD
Keywords - Cellular componenti
Cytoplasm, Endoplasmic reticulum, Membrane, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 323 | G → S in npl4-1; nuclear-targeted proteins accumulate in the cytoplasm. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000057944 | 1 – 580 | Nuclear protein localization protein 4Add BLAST | 580 |
Proteomic databases
MaxQBi | P33755 |
PaxDbi | P33755 |
PRIDEi | P33755 |
PTM databases
iPTMneti | P33755 |
Interactioni
Subunit structurei
Component of the HRD1 complex which contains HRD1, HRD3, USA1, DER1, YOS9, CDC48, NPL4, UFD1 AND UBX2/SEL1. The complex is composed of the core membrane complex, consisting of the E3 ligase HRD1 and its cofactors HRD3, DER1 and USA1, the substrate recruiting factor YOS9, and the heterotrimeric UFD1-NPL4-CDC48/p97 (UNC) ATPase complex recruited by UBX2/SEL1 (PubMed:16873066).
Forms a complex composed of CDC48, NPL4, UFD1, DOA1, SHP1 and deubiquitinase OTU1 (PubMed:16427015).
Interacts with CDC48, UFD1 and VMS1 (PubMed:11598205, PubMed:11733065, PubMed:11739805, PubMed:21070972, PubMed:31249135).
7 PublicationsBinary interactionsi
Hide detailsP33755
With | #Exp. | IntAct |
---|---|---|
CDC48 [P25694] | 11 | EBI-12193,EBI-4308 |
UFD1 [P53044] | 11 | EBI-12193,EBI-19997 |
GO - Molecular functioni
- ubiquitin binding Source: GO_Central
- ubiquitin protein ligase binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 32870, 259 interactors |
ComplexPortali | CPX-2946, CDC48-NPL4-UFD1 AAA ATPase complex CPX-3069, CDC48-NPL4-VMS1 AAA ATPase complex CPX-3265, Ribosome quality control complex |
DIPi | DIP-1475N |
IntActi | P33755, 18 interactors |
MINTi | P33755 |
STRINGi | 4932.YBR170C |
Miscellaneous databases
RNActi | P33755, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P33755 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 237 – 377 | MPNPROSITE-ProRule annotationAdd BLAST | 141 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2834, Eukaryota |
GeneTreei | ENSGT00390000018731 |
HOGENOMi | CLU_017172_0_0_1 |
InParanoidi | P33755 |
OMAi | KMCRHGP |
Family and domain databases
CDDi | cd08061, MPN_NPL4, 1 hit |
InterProi | View protein in InterPro IPR037518, MPN IPR016563, Npl4 IPR007717, NPL4_C IPR007716, NPL4_Zn-bd_put IPR029071, Ubiquitin-like_domsf |
Pfami | View protein in Pfam PF05021, NPL4, 1 hit PF05020, zf-NPL4, 1 hit |
PIRSFi | PIRSF010052, Polyub_prc_Npl4, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS50249, MPN, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MLIRFRSKNG THRVSCQEND LFGTVIEKLV GNLDPNADVD TFTVCEKPGQ
60 70 80 90 100
GIHAVSELAD RTVMDLGLKH GDMLILNYSD KPANEKDGVN VEIGSVGIDS
110 120 130 140 150
KGIRQHRYGP LRIKELAVDE ELEKEDGLIP RQKSKLCKHG DRGMCEYCSP
160 170 180 190 200
LPPWDKEYHE KNKIKHISFH SYLKKLNENA NKKENGSSYI SPLSEPDFRI
210 220 230 240 250
NKRCHNGHEP WPRGICSKCQ PSAITLQQQE FRMVDHVEFQ KSEIINEFIQ
260 270 280 290 300
AWRYTGMQRF GYMYGSYSKY DNTPLGIKAV VEAIYEPPQH DEQDGLTMDV
310 320 330 340 350
EQVKNEMLQI DRQAQEMGLS RIGLIFTDLS DAGAGDGSVF CKRHKDSFFL
360 370 380 390 400
SSLEVIMAAR HQTRHPNVSK YSEQGFFSSK FVTCVISGNL EGEIDISSYQ
410 420 430 440 450
VSTEAEALVT ADMISGSTFP SMAYINDTTD ERYVPEIFYM KSNEYGITVK
460 470 480 490 500
ENAKPAFPVD YLLVTLTHGF PNTDTETNSK FVSSTGFPWS NRQAMGQSQD
510 520 530 540 550
YQELKKYLFN VASSGDFNLL HEKISNFHLL LYINSLQILS PDEWKLLIES
560 570 580
AVKNEWEESL LKLVSSAGWQ TLVMILQESG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X72224 Genomic DNA Translation: CAA51026.1 X74437 Genomic DNA Translation: CAA52450.1 Z36039 Genomic DNA Translation: CAA85131.1 BK006936 Genomic DNA Translation: DAA07286.1 |
PIRi | S34340 |
RefSeqi | NP_009729.1, NM_001178518.1 |
Genome annotation databases
EnsemblFungii | YBR170C_mRNA; YBR170C; YBR170C |
GeneIDi | 852468 |
KEGGi | sce:YBR170C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X72224 Genomic DNA Translation: CAA51026.1 X74437 Genomic DNA Translation: CAA52450.1 Z36039 Genomic DNA Translation: CAA85131.1 BK006936 Genomic DNA Translation: DAA07286.1 |
PIRi | S34340 |
RefSeqi | NP_009729.1, NM_001178518.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6JWH | X-ray | 1.72 | A | 113-580 | [»] | |
6JWI | X-ray | 2.55 | A/E | 113-580 | [»] | |
6JWJ | X-ray | 1.58 | A | 113-580 | [»] | |
6OA9 | electron microscopy | 3.90 | G | 1-580 | [»] | |
6OAA | electron microscopy | 4.10 | G | 1-580 | [»] | |
SMRi | P33755 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 32870, 259 interactors |
ComplexPortali | CPX-2946, CDC48-NPL4-UFD1 AAA ATPase complex CPX-3069, CDC48-NPL4-VMS1 AAA ATPase complex CPX-3265, Ribosome quality control complex |
DIPi | DIP-1475N |
IntActi | P33755, 18 interactors |
MINTi | P33755 |
STRINGi | 4932.YBR170C |
Protein family/group databases
TCDBi | 3.A.16.1.2, the endoplasmic reticular retrotranslocon (er-rt) family |
PTM databases
iPTMneti | P33755 |
Proteomic databases
MaxQBi | P33755 |
PaxDbi | P33755 |
PRIDEi | P33755 |
Genome annotation databases
EnsemblFungii | YBR170C_mRNA; YBR170C; YBR170C |
GeneIDi | 852468 |
KEGGi | sce:YBR170C |
Organism-specific databases
SGDi | S000000374, NPL4 |
VEuPathDBi | FungiDB:YBR170C |
Phylogenomic databases
eggNOGi | KOG2834, Eukaryota |
GeneTreei | ENSGT00390000018731 |
HOGENOMi | CLU_017172_0_0_1 |
InParanoidi | P33755 |
OMAi | KMCRHGP |
Miscellaneous databases
PROi | PR:P33755 |
RNActi | P33755, protein |
Family and domain databases
CDDi | cd08061, MPN_NPL4, 1 hit |
InterProi | View protein in InterPro IPR037518, MPN IPR016563, Npl4 IPR007717, NPL4_C IPR007716, NPL4_Zn-bd_put IPR029071, Ubiquitin-like_domsf |
Pfami | View protein in Pfam PF05021, NPL4, 1 hit PF05020, zf-NPL4, 1 hit |
PIRSFi | PIRSF010052, Polyub_prc_Npl4, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS50249, MPN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NPL4_YEAST | |
Accessioni | P33755Primary (citable) accession number: P33755 Secondary accession number(s): D6VQG6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | February 1, 1994 | |
Last modified: | February 10, 2021 | |
This is version 161 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome II
Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families