UniProtKB - P33644 (PURNU_ECOLI)
Protein
Purine nucleoside phosphorylase YfiH
Gene
yfiH
Organism
Escherichia coli (strain K12)
Status
Functioni
Purine nucleoside enzyme that catalyzes the phosphorolysis of adenosine and inosine nucleosides, yielding D-ribose 1-phosphate and the respective free bases, adenine and hypoxanthine (PubMed:31978345). Also catalyzes the phosphorolysis of S-methyl-5'-thioadenosine into adenine and S-methyl-5-thio-alpha-D-ribose 1-phosphate (PubMed:31978345). Also has adenosine deaminase activity (PubMed:31978345). May also act as a polyphenol oxidase: able to oxidize syringaldazine and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) in vitro (PubMed:16740638).2 Publications
Catalytic activityi
- EC:2.4.2.11 PublicationThis reaction proceeds in the forward1 Publication direction.
- phosphate + S-methyl-5'-thioadenosine = adenine + S-methyl-5-thio-α-D-ribose 1-phosphate1 PublicationEC:2.4.2.281 PublicationThis reaction proceeds in the forward1 Publication direction.
- EC:2.4.2.11 PublicationThis reaction proceeds in the forward1 Publication direction.
- EC:3.5.4.41 PublicationThis reaction proceeds in the forward1 Publication direction.
Cofactori
Kineticsi
kcat is 1450 min(-1) with 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid as substrate. kcat is 21720 min(-1) with syringaldazine as substrate (at pH 4.5 and 40 degrees Celsius).1 Publication
- KM=23 µM for 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid (at pH 4.5 and 40 degrees Celsius)1 Publication
- KM=1.10 µM for syringaldazine (at pH 4.5 and 40 degrees Celsius)1 Publication
pH dependencei
Optimum pH is 5.5-8.4. Maintains 80% activity at pH 5.0-9.0.1 Publication
Temperature dependencei
Optimum temperature is 44 degrees Celsius. Maintains more than 80% activity at 50 degrees Celsius.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 71 | Copper or zinc; catalyticBy similarity | 1 | |
Metal bindingi | 107 | Copper or zinc; catalyticBy similarity | 1 | |
Metal bindingi | 124 | Copper or zinc; catalyticBy similarity | 1 |
GO - Molecular functioni
- adenosine deaminase activity Source: UniProtKB
- copper ion binding Source: EcoCyc
- oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
- purine-nucleoside phosphorylase activity Source: UniProtKB
- S-methyl-5-thioadenosine phosphorylase activity Source: UniProtKB
Keywordsi
Molecular function | Hydrolase, Oxidoreductase, Transferase |
Ligand | Copper, Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12097-MONOMER MetaCyc:EG12097-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Purine nucleoside phosphorylase YfiHCurated (EC:2.4.2.11 Publication)Alternative name(s): |
Gene namesi | Name:yfiH1 PublicationImported Ordered Locus Names:b2593, JW2575 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Cells lacking this gene are more sensitive to ampicillin, cephradine and cefoxitin than wild-type.1 Publication
Chemistry databases
DrugBanki | DB04272, Citric acid |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000163163 | 1 – 243 | Purine nucleoside phosphorylase YfiHAdd BLAST | 243 |
Proteomic databases
jPOSTi | P33644 |
PaxDbi | P33644 |
PRIDEi | P33644 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationGO - Molecular functioni
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 4260619, 654 interactors |
IntActi | P33644, 8 interactors |
STRINGi | 511145.b2593 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P33644 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P33644 |
Family & Domainsi
Sequence similaritiesi
Belongs to the purine nucleoside phosphorylase YfiH/LACC1 family.Curated
Phylogenomic databases
eggNOGi | COG1496, Bacteria |
HOGENOMi | CLU_065784_1_1_6 |
InParanoidi | P33644 |
PhylomeDBi | P33644 |
Family and domain databases
CDDi | cd16833, YfiH, 1 hit |
Gene3Di | 3.60.140.10, 1 hit |
InterProi | View protein in InterPro IPR003730, Cu_polyphenol_OxRdtase IPR038371, Cu_polyphenol_OxRdtase_sf IPR011324, Cytotoxic_necrot_fac-like_cat |
PANTHERi | PTHR30616, PTHR30616, 1 hit |
Pfami | View protein in Pfam PF02578, Cu-oxidase_4, 1 hit |
SUPFAMi | SSF64438, SSF64438, 1 hit |
TIGRFAMsi | TIGR00726, TIGR00726, 1 hit |
i Sequence
Sequence statusi: Complete.
P33644-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSKLIVPQWP QPKGVAACSS TRIGGVSLPP YDSLNLGAHC GDNPDHVEEN
60 70 80 90 100
RKRLFAAGNL PSKPVWLEQV HGKDVLKLTG EPYASKRADA SYSNTPGTVC
110 120 130 140 150
AVMTADCLPV LFCNRAGTEV AAAHAGWRGL CAGVLEETVS CFADNPENIL
160 170 180 190 200
AWLGPAIGPR AFEVGGEVRE AFMAVDAKAS AAFIQHGDKY LADIYQLARQ
210 220 230 240
RLANVGVEQI FGGDRCTYTE NETFFSYRRD KTTGRMASFI WLI
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 27 | S → T in AAA92958 (Ref. 1) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U50134 Genomic DNA Translation: AAA92958.1 X57620 Genomic DNA No translation available. U00096 Genomic DNA Translation: AAC75642.1 AP009048 Genomic DNA Translation: BAA16477.1 M29364 Genomic DNA Translation: AAA24421.1 Sequence problems. |
PIRi | D65037 |
RefSeqi | NP_417084.1, NC_000913.3 WP_000040169.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75642; AAC75642; b2593 BAA16477; BAA16477; BAA16477 |
GeneIDi | 57728725 947089 |
KEGGi | ecj:JW2575 eco:b2593 |
PATRICi | fig|511145.12.peg.2693 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U50134 Genomic DNA Translation: AAA92958.1 X57620 Genomic DNA No translation available. U00096 Genomic DNA Translation: AAC75642.1 AP009048 Genomic DNA Translation: BAA16477.1 M29364 Genomic DNA Translation: AAA24421.1 Sequence problems. |
PIRi | D65037 |
RefSeqi | NP_417084.1, NC_000913.3 WP_000040169.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1Z9T | X-ray | 1.54 | A | 1-243 | [»] | |
SMRi | P33644 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260619, 654 interactors |
IntActi | P33644, 8 interactors |
STRINGi | 511145.b2593 |
Chemistry databases
DrugBanki | DB04272, Citric acid |
Proteomic databases
jPOSTi | P33644 |
PaxDbi | P33644 |
PRIDEi | P33644 |
Genome annotation databases
EnsemblBacteriai | AAC75642; AAC75642; b2593 BAA16477; BAA16477; BAA16477 |
GeneIDi | 57728725 947089 |
KEGGi | ecj:JW2575 eco:b2593 |
PATRICi | fig|511145.12.peg.2693 |
Organism-specific databases
EchoBASEi | EB2021 |
Phylogenomic databases
eggNOGi | COG1496, Bacteria |
HOGENOMi | CLU_065784_1_1_6 |
InParanoidi | P33644 |
PhylomeDBi | P33644 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12097-MONOMER MetaCyc:EG12097-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P33644 |
PROi | PR:P33644 |
Family and domain databases
CDDi | cd16833, YfiH, 1 hit |
Gene3Di | 3.60.140.10, 1 hit |
InterProi | View protein in InterPro IPR003730, Cu_polyphenol_OxRdtase IPR038371, Cu_polyphenol_OxRdtase_sf IPR011324, Cytotoxic_necrot_fac-like_cat |
PANTHERi | PTHR30616, PTHR30616, 1 hit |
Pfami | View protein in Pfam PF02578, Cu-oxidase_4, 1 hit |
SUPFAMi | SSF64438, SSF64438, 1 hit |
TIGRFAMsi | TIGR00726, TIGR00726, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PURNU_ECOLI | |
Accessioni | P33644Primary (citable) accession number: P33644 Secondary accession number(s): Q46989 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | February 1, 1994 | |
Last modified: | April 7, 2021 | |
This is version 144 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families