UniProtKB - P33643 (RLUD_ECOLI)
Protein
Ribosomal large subunit pseudouridine synthase D
Gene
rluD
Organism
Escherichia coli (strain K12)
Status
Functioni
Responsible for synthesis of pseudouridine from uracil at positions 1911, 1915 and 1917 in 23S ribosomal RNA. Isomerization occurs as a late step during the assembly of the large ribosomal subunit.2 Publications
Catalytic activityi
- EC:5.4.99.232 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 139 | 1 |
GO - Molecular functioni
- pseudouridine synthase activity Source: GO_Central
- RNA binding Source: UniProtKB-KW
- rRNA pseudouridine synthase activity Source: EcoCyc
GO - Biological processi
- enzyme-directed rRNA pseudouridine synthesis Source: EcoCyc
- ribosomal large subunit assembly Source: EcoCyc
Keywordsi
Molecular function | Isomerase, RNA-binding |
Biological process | rRNA processing |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12098-MONOMER MetaCyc:EG12098-MONOMER |
BRENDAi | 5.4.99.23, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: Ribosomal large subunit pseudouridine synthase D (EC:5.4.99.23)Alternative name(s): 23S rRNA pseudouridine(1911/1915/1917) synthase rRNA pseudouridylate synthase D rRNA-uridine isomerase D |
Gene namesi | Name:rluD Synonyms:sfhB, yfiI Ordered Locus Names:b2594, JW2576 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 139 | D → N or T: Loss of activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed | |||
ChainiPRO_0000162688 | 2 – 326 | Ribosomal large subunit pseudouridine synthase DAdd BLAST | 325 |
Proteomic databases
jPOSTi | P33643 |
PaxDbi | P33643 |
PRIDEi | P33643 |
Interactioni
Binary interactionsi
Hide detailsProtein-protein interaction databases
BioGRIDi | 4263197, 106 interactors |
DIPi | DIP-10721N |
IntActi | P33643, 18 interactors |
STRINGi | 511145.b2594 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P33643 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P33643 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 18 – 91 | S4 RNA-bindingPROSITE-ProRule annotationAdd BLAST | 74 |
Sequence similaritiesi
Belongs to the pseudouridine synthase RluA family.Curated
Phylogenomic databases
eggNOGi | COG0564, Bacteria |
HOGENOMi | CLU_016902_4_0_6 |
InParanoidi | P33643 |
PhylomeDBi | P33643 |
Family and domain databases
CDDi | cd02869, PseudoU_synth_RluCD_like, 1 hit cd00165, S4, 1 hit |
Gene3Di | 3.10.290.10, 1 hit |
InterProi | View protein in InterPro IPR020103, PsdUridine_synth_cat_dom_sf IPR006225, PsdUridine_synth_RluC/D IPR006224, PsdUridine_synth_RluC/D_CS IPR006145, PsdUridine_synth_RsuA/RluA IPR002942, S4_RNA-bd IPR036986, S4_RNA-bd_sf |
Pfami | View protein in Pfam PF00849, PseudoU_synth_2, 1 hit PF01479, S4, 1 hit |
SMARTi | View protein in SMART SM00363, S4, 1 hit |
SUPFAMi | SSF55120, SSF55120, 1 hit |
TIGRFAMsi | TIGR00005, rluA_subfam, 1 hit |
PROSITEi | View protein in PROSITE PS01129, PSI_RLU, 1 hit PS50889, S4, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P33643-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAQRVQLTAT VSENQLGQRL DQALAEMFPD YSRSRIKEWI LDQRVLVNGK
60 70 80 90 100
VCDKPKEKVL GGEQVAINAE IEEEARFEPQ DIPLDIVYED EDIIIINKPR
110 120 130 140 150
DLVVHPGAGN PDGTVLNALL HYYPPIADVP RAGIVHRLDK DTTGLMVVAK
160 170 180 190 200
TVPAQTRLVE SLQRREITRE YEAVAIGHMT AGGTVDEPIS RHPTKRTHMA
210 220 230 240 250
VHPMGKPAVT HYRIMEHFRV HTRLRLRLET GRTHQIRVHM AHITHPLVGD
260 270 280 290 300
PVYGGRPRPP KGASEAFIST LRKFDRQALH ATMLRLYHPI SGIEMEWHAP
310 320
IPQDMVELIE VMRADFEEHK DEVDWL
Sequence cautioni
The sequence X57620 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 271 – 326 | LRKFD…EVDWL → AGVSLTARRYMQPCCVFITR SPASKWNGMRLFHKIWWS (PubMed:9205837).CuratedAdd BLAST | 56 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U50134 Genomic DNA Translation: AAA92957.1 U00096 Genomic DNA Translation: AAC75643.1 AP009048 Genomic DNA Translation: BAA16479.2 X57620 Genomic DNA No translation available. |
PIRi | E65037 |
RefSeqi | NP_417085.1, NC_000913.3 WP_000079100.1, NZ_STEB01000040.1 |
Genome annotation databases
EnsemblBacteriai | AAC75643; AAC75643; b2594 BAA16479; BAA16479; BAA16479 |
GeneIDi | 57728724 947087 |
KEGGi | ecj:JW2576 eco:b2594 |
PATRICi | fig|1411691.4.peg.4143 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U50134 Genomic DNA Translation: AAA92957.1 U00096 Genomic DNA Translation: AAC75643.1 AP009048 Genomic DNA Translation: BAA16479.2 X57620 Genomic DNA No translation available. |
PIRi | E65037 |
RefSeqi | NP_417085.1, NC_000913.3 WP_000079100.1, NZ_STEB01000040.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1PRZ | X-ray | 1.80 | A | 75-326 | [»] | |
1QYU | X-ray | 2.00 | A | 2-326 | [»] | |
1V9F | X-ray | 1.70 | A | 2-326 | [»] | |
2IST | X-ray | 1.86 | A | 2-326 | [»] | |
SMRi | P33643 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4263197, 106 interactors |
DIPi | DIP-10721N |
IntActi | P33643, 18 interactors |
STRINGi | 511145.b2594 |
Proteomic databases
jPOSTi | P33643 |
PaxDbi | P33643 |
PRIDEi | P33643 |
Genome annotation databases
EnsemblBacteriai | AAC75643; AAC75643; b2594 BAA16479; BAA16479; BAA16479 |
GeneIDi | 57728724 947087 |
KEGGi | ecj:JW2576 eco:b2594 |
PATRICi | fig|1411691.4.peg.4143 |
Organism-specific databases
EchoBASEi | EB2022 |
Phylogenomic databases
eggNOGi | COG0564, Bacteria |
HOGENOMi | CLU_016902_4_0_6 |
InParanoidi | P33643 |
PhylomeDBi | P33643 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12098-MONOMER MetaCyc:EG12098-MONOMER |
BRENDAi | 5.4.99.23, 2026 |
Miscellaneous databases
EvolutionaryTracei | P33643 |
PROi | PR:P33643 |
Family and domain databases
CDDi | cd02869, PseudoU_synth_RluCD_like, 1 hit cd00165, S4, 1 hit |
Gene3Di | 3.10.290.10, 1 hit |
InterProi | View protein in InterPro IPR020103, PsdUridine_synth_cat_dom_sf IPR006225, PsdUridine_synth_RluC/D IPR006224, PsdUridine_synth_RluC/D_CS IPR006145, PsdUridine_synth_RsuA/RluA IPR002942, S4_RNA-bd IPR036986, S4_RNA-bd_sf |
Pfami | View protein in Pfam PF00849, PseudoU_synth_2, 1 hit PF01479, S4, 1 hit |
SMARTi | View protein in SMART SM00363, S4, 1 hit |
SUPFAMi | SSF55120, SSF55120, 1 hit |
TIGRFAMsi | TIGR00005, rluA_subfam, 1 hit |
PROSITEi | View protein in PROSITE PS01129, PSI_RLU, 1 hit PS50889, S4, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RLUD_ECOLI | |
Accessioni | P33643Primary (citable) accession number: P33643 Secondary accession number(s): P77003 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 10, 2021 | |
This is version 173 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families